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Protein

Polyamine oxidase 3

Gene

PAO3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Flavoenzyme that catalyzes the sequential oxidation of spermine to spermidine, and of spermidine to putrescine. Substrate preference is spermidine > spermine > N(1)-acetylspermidine > N(1)-acetylspermine. No activity detected when diamines (agmatine, cadaverine or putrescine) or N(8)-acetylspermidine are used as substrates.1 Publication

Catalytic activityi

Spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2.1 Publication
Spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2.1 Publication
N(1)-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2.1 Publication
N(1)-acetylspermidine + O2 + H2O = putrescine + 3-acetamidopropanal + H2O2.1 Publication

Cofactori

FADNote: Binds 1 FAD per subunit.

Enzyme regulationi

Inhibited by guazatine, aminoguanidine and putrescine.1 Publication

Kineticsi

  1. KM=0.204 mM for spermidine1 Publication
  2. KM=0.588 mM for spermine1 Publication
  3. KM=1 mM for N(1)-acetylspermidine1 Publication
  4. KM=2 mM for N(1)-acetylspermine1 Publication

    pH dependencei

    Optimum pH is 7.5. No activity at pH above 9.5 or below 6.5.1 Publication

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.1 Publication

    Pathwayi: spermine degradation

    This protein is involved in the pathway spermine degradation, which is part of Amine and polyamine degradation.
    View all proteins of this organism that are known to be involved in the pathway spermine degradation and in Amine and polyamine degradation.

    GO - Molecular functioni

    GO - Biological processi

    • polyamine catabolic process Source: TAIR
    • spermine catabolic process Source: UniProtKB-UniPathway
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciARA:AT3G59050-MONOMER.
    MetaCyc:AT3G59050-MONOMER.
    BRENDAi1.5.3.17. 399.
    UniPathwayiUPA00211.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyamine oxidase 3 (EC:1.5.3.17)
    Short name:
    AtPAO3
    Gene namesi
    Name:PAO3
    Ordered Locus Names:At3g59050
    ORF Names:F17J16.100
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 3

    Organism-specific databases

    TAIRiAT3G59050.

    Subcellular locationi

    GO - Cellular componenti

    • peroxisome Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Polyamine oxidase 3PRO_0000352509Add
    BLAST

    Proteomic databases

    PaxDbiQ9LYT1.
    PRIDEiQ9LYT1.

    Expressioni

    Tissue specificityi

    Expressed at similar levels in all tissues tested. Highest expression is seen in siliques.1 Publication

    Inductioni

    By abscisic acid, jasmonate, salicylic acid, wounding and flagellin 22, a pathogen elicitor.1 Publication

    Gene expression databases

    GenevisibleiQ9LYT1. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi10389. 5 interactions.
    IntActiQ9LYT1. 1 interaction.
    STRINGi3702.AT3G59050.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LYT1.
    SMRiQ9LYT1. Positions 24-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family.Curated

    Phylogenomic databases

    eggNOGiENOG410IUNJ. Eukaryota.
    ENOG410XQHS. LUCA.
    HOGENOMiHOG000037651.
    InParanoidiQ9LYT1.
    KOiK17839.
    OMAiASTGRNW.
    OrthoDBiEOG093607H7.
    PhylomeDBiQ9LYT1.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    InterProiIPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.
    SUPFAMiSSF51905. SSF51905. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9LYT1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MESGGKTNRQ LRKAICVSTD EKMKKKRSPS VIVIGGGMAG ISAARTLQDA
    60 70 80 90 100
    SFQVVVLESR DRIGGRVHTD YSFGFPVDLG ASWLHGVCKE NPLAAVIGRL
    110 120 130 140 150
    GLPLYRTSGD NSVLYDHDLE SYALFDKAGN QVSQELVTKV GENFEHILEE
    160 170 180 190 200
    ICKVRDEQDE DMSIAQAFSI VFKRNPELRL EGLAHNVLQW YLCRMEGWFA
    210 220 230 240 250
    ADAETISAKC WDQEELLPGG HGLMVRGYRP VINTLSKGLD IRLSHRITKI
    260 270 280 290 300
    SRRYSGVKVT TEKGDTFVAD AAVIALPLGV LKSGMITFEP KLPQWKQEAI
    310 320 330 340 350
    NDLGVGIENK IILNFDNVFW PNVEFLGVVA ETSYGCSYFL NLHKATSHPV
    360 370 380 390 400
    LVYMPAGQLA RDIEKKSDEA AANFAFSQLQ KILPDASSPI NYLVSRWGSD
    410 420 430 440 450
    INSLGSYSYD IVNKPHDLYE RLRVPLDNLF FAGEATSSSY PGSVHGAYST
    460 470 480
    GVLAAEDCRM RVLERYGELE HEMEEEAPAS VPLLISRM
    Length:488
    Mass (Da):54,132
    Last modified:October 1, 2000 - v1
    Checksum:i1EC37D18794A2BD4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti247 – 2471I → V in AAM62855 (Ref. 4) Curated
    Sequence conflicti366 – 3661K → N in AAM62855 (Ref. 4) Curated
    Sequence conflicti404 – 4041L → M in AAM62855 (Ref. 4) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL163527 Genomic DNA. Translation: CAB86933.1.
    CP002686 Genomic DNA. Translation: AEE79866.1.
    AY065025 mRNA. Translation: AAL57665.1.
    AY143905 mRNA. Translation: AAN28844.1.
    AY085634 mRNA. Translation: AAM62855.1.
    PIRiT47787.
    RefSeqiNP_191464.1. NM_115767.3.
    UniGeneiAt.27590.

    Genome annotation databases

    EnsemblPlantsiAT3G59050.1; AT3G59050.1; AT3G59050.
    GeneIDi825074.
    GrameneiAT3G59050.1; AT3G59050.1; AT3G59050.
    KEGGiath:AT3G59050.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL163527 Genomic DNA. Translation: CAB86933.1.
    CP002686 Genomic DNA. Translation: AEE79866.1.
    AY065025 mRNA. Translation: AAL57665.1.
    AY143905 mRNA. Translation: AAN28844.1.
    AY085634 mRNA. Translation: AAM62855.1.
    PIRiT47787.
    RefSeqiNP_191464.1. NM_115767.3.
    UniGeneiAt.27590.

    3D structure databases

    ProteinModelPortaliQ9LYT1.
    SMRiQ9LYT1. Positions 24-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi10389. 5 interactions.
    IntActiQ9LYT1. 1 interaction.
    STRINGi3702.AT3G59050.1.

    Proteomic databases

    PaxDbiQ9LYT1.
    PRIDEiQ9LYT1.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT3G59050.1; AT3G59050.1; AT3G59050.
    GeneIDi825074.
    GrameneiAT3G59050.1; AT3G59050.1; AT3G59050.
    KEGGiath:AT3G59050.

    Organism-specific databases

    TAIRiAT3G59050.

    Phylogenomic databases

    eggNOGiENOG410IUNJ. Eukaryota.
    ENOG410XQHS. LUCA.
    HOGENOMiHOG000037651.
    InParanoidiQ9LYT1.
    KOiK17839.
    OMAiASTGRNW.
    OrthoDBiEOG093607H7.
    PhylomeDBiQ9LYT1.

    Enzyme and pathway databases

    UniPathwayiUPA00211.
    BioCyciARA:AT3G59050-MONOMER.
    MetaCyc:AT3G59050-MONOMER.
    BRENDAi1.5.3.17. 399.

    Miscellaneous databases

    PROiQ9LYT1.

    Gene expression databases

    GenevisibleiQ9LYT1. AT.

    Family and domain databases

    Gene3Di3.50.50.60. 3 hits.
    InterProiIPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.
    SUPFAMiSSF51905. SSF51905. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPAO3_ARATH
    AccessioniPrimary (citable) accession number: Q9LYT1
    Secondary accession number(s): Q8LE44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: October 1, 2000
    Last modified: September 7, 2016
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.