Reviewed, UniProtKB/Swiss-Prot Q9LYS6 (CALS6_ARATH)

Names and origin

Protein names

Recommended name:
    Putative callose synthase 6
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 11

Gene names

Name:	CALS6
Synonyms:	GSL11
Ordered Locus Names:	At3g59100
ORF Names:	F17J16.150

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1921 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Probably involved in callose synthesis, but not required for callose formation after wounding or pathogen attack. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.3
Catalytic activity	UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 48 family.
Sequence caution	The sequence CAB86938.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	1,3-beta-glucan synthase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,3-beta-glucan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1921	1921	Putative callose synthase 6		PRO_0000334578
Regions
	Topological domain	1 – 492	492	Cytoplasmic Potential
	Transmembrane	493 – 513	21	Potential
	Topological domain	514 – 526	13	Extracellular Potential
	Transmembrane	527 – 547	21	Potential
	Topological domain	548 – 560	13	Cytoplasmic Potential
	Transmembrane	561 – 581	21	Potential
	Topological domain	582 – 611	30	Extracellular Potential
	Transmembrane	612 – 632	21	Potential
	Topological domain	633 – 674	42	Cytoplasmic Potential
	Transmembrane	675 – 695	21	Potential
	Topological domain	696 – 721	26	Extracellular Potential
	Transmembrane	722 – 742	21	Potential
	Topological domain	743 – 1484	742	Cytoplasmic Potential
	Transmembrane	1485 – 1505	21	Potential
	Topological domain	1506 – 1540	35	Extracellular Potential
	Transmembrane	1541 – 1561	21	Potential
	Topological domain	1562 – 1564	3	Cytoplasmic Potential
	Transmembrane	1565 – 1585	21	Potential
	Topological domain	1586 – 1628	43	Extracellular Potential
	Transmembrane	1629 – 1649	21	Potential
	Topological domain	1650 – 1655	6	Cytoplasmic Potential
	Transmembrane	1656 – 1676	21	Potential
	Topological domain	1677 – 1730	54	Extracellular Potential
	Transmembrane	1731 – 1751	21	Potential
	Topological domain	1752 – 1759	8	Cytoplasmic Potential
	Transmembrane	1760 – 1780	21	Potential
	Topological domain	1781 – 1796	16	Extracellular Potential
	Transmembrane	1797 – 1817	21	Potential
	Topological domain	1818 – 1823	6	Cytoplasmic Potential
	Transmembrane	1824 – 1844	21	Potential
	Topological domain	1845 – 1867	23	Extracellular Potential
	Transmembrane	1868 – 1888	21	Potential
	Topological domain	1889 – 1921	33	Cytoplasmic Potential
	Compositional bias	103 – 108	6	Poly-Glu
	Compositional bias	1633 – 1639	7	Poly-Leu

Sequences

	Sequence		Length	Mass (Da)
	Q9LYS6-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: F2CC970B984F9710	FASTA	1,921	223,514
	10 20 30 40 50 60 MEASSSGTAE LPRSLSRRAP SRATTMMIDR PNEDASAMDS ELVPSSLASI APILRVANEI 70 80 90 100 110 120 EKDNPRVAYL CRFHAFEKAH RMDATSSGRG VRQFKTYLLH RLEKEEEETK PQLAKNDPRE 130 140 150 160 170 180 IQAYYQNFYE KYIKEGETSR KPEEMARLYQ IASVLYDVLK TVVPSPKVDY ETRRYAEEVE 190 200 210 220 230 240 RKRDRYEHYN ILPLYAVGTK PAIVELPEVK AAFSAVRNVR NLPRRRIHLP SNTPNEMRKA 250 260 270 280 290 300 RTKLNDILEW LASEFGFQRG NVANQREHII LLLANADIRK RNDEEYDELK PSTVTELMDK 310 320 330 340 350 360 TFKSYYSWCK YLHSTSNLKF PDDCDKQQLQ LIYISLYLLI WGEASNVRFM PECICYIFHN 370 380 390 400 410 420 MANDVYGILF SNVEAVSGET YETEEVIDEE SFLRTVITPI YQVIRNEAKR NKGGTASHSQ 430 440 450 460 470 480 WRNYDDLNEY FWSKKCFKIG WPLDLKADFF LNSDEITPQD ERLNQVTYGK SKPKTNFVEV 490 500 510 520 530 540 RTFWNLFRDF DRMWIFLVMA FQAMVIVGWH GSGSLGDIFD KDVFKTVLTI FITSAYLTLL 550 560 570 580 590 600 QAALDIILNF NAWKNFKFSQ ILRYLLKFAV AFMWAVLLPI AYSKSVQRPT GVVKFFSTWT 610 620 630 640 650 660 GDWKDQSFYT YAVSFYVLPN ILAALLFLVP PFRRAMECSD MRPIKVIMWW AQPKLYVGRG 670 680 690 700 710 720 MHEDMFSLFK YTTFWIMLLI SKLAFNYYVE ILPLITPTKM IMNLHIGHYQ WHEFFPHATN 730 740 750 760 770 780 NIGVVIAIWA PIVLVYLMDT QIWYAIFSTL FGGIHGAFSH LGEIRTLGML RSRFESIPIA 790 800 810 820 830 840 FSRTLMPSED AKRKHADDYV DQKNITNFSQ VWNEFIYSMR SEDKISDRDR DLLLVPSSSG 850 860 870 880 890 900 DVSVIQWPPF LLASKIPIAV DMAKDFKGKE DAELFRKIKS DSYMYYAVIE SYETLKKIIY 910 920 930 940 950 960 ALLEDEADRR VMNQVFLEVD MSMQQQRFIY EFRMSGLPLL SDKLEKFLSI LLSDYEDQGT 970 980 990 1000 1010 1020 YKSQLINVFQ DVIEIITQDL LVNGHEILER ARVHSPDIKN EKKEQRFEKI NIHLVRDRCW 1030 1040 1050 1060 1070 1080 REKVIRLHLL LSVKESAINV PQNLEARRRI TFFANSLFMN MPSAPRIRDM LSFSVLTPYY 1090 1100 1110 1120 1130 1140 KEDVLYSEED LNKENEDGIS ILFYLQKIYP DEWTNYLDRL KDPKLPEKDK SEFLREWVSY 1150 1160 1170 1180 1190 1200 RGQTLARTVR GMMYYRQALE LQCYQEVAGE QAEFSVFRAM ASNDENQKAF LERARALADL 1210 1220 1230 1240 1250 1260 KFTYVVSCQV YGNQKKSGDI HNRSCYTNIL QLMLKYPSLR VAYVDEREET ADAKSPKVFY 1270 1280 1290 1300 1310 1320 SVLLKGGDKF DEEIYRIKLP GPPAEIGEGK PENQNHAIIF TRGEALQTID MNQDNYFEEA 1330 1340 1350 1360 1370 1380 FKLRNVLEEF NKERVGRRKP TILGLREHIF TGSVSSLAWF MSNQESSFVT IGQRILANPL 1390 1400 1410 1420 1430 1440 RVRFHYGHPD IFDRIFHITR GGVSKASKVI NLSEDIFGGF NSTLRGGYVT HHEYIQVGKG 1450 1460 1470 1480 1490 1500 RDVGLNPISI FEAKVANGNG EQTLSRDVYR LGHRFDFYRM LSFYFTTIGF YFSSMLTVLT 1510 1520 1530 1540 1550 1560 VYAFLYGRMY MVMSGLEKEI LRLASPNQLE ALEQALATQS IFQLGFLMVL PMVMEIGLEH 1570 1580 1590 1600 1610 1620 GFRSAIVDFF IMQLQLASVF FTFQLGTKSH YYGRTILHGG SKYRPTGRGF VVFHAKFAEN 1630 1640 1650 1660 1670 1680 YRLYSRSHFV KGLELLLLLV VYQIYGHSYR SSNLYLYITV SMWFMVGSWL FAPFIFNPSG 1690 1700 1710 1720 1730 1740 FEWQKTVDDW TDWKRWLGDR GGIGIPVEKS WESWWNVEQE HLKHTSIRGR ILEITLALRF 1750 1760 1770 1780 1790 1800 FIYQYGIVYQ LNISQRSKSF LVYGLSWVVL LTSLLVLKMV SMGRRRFGTD FQLMFRILKA 1810 1820 1830 1840 1850 1860 LLFLGFLSVM TILFVVFKLT LTDLSASVLA FLPTGWAILL IGQVLRSPIK ALGVWDSVKE 1870 1880 1890 1900 1910 1920 LGRAYENIMG LVIFAPIAVL SWFPIVSEFQ ARLLFNQAFS RGLQISMILA GRKDKATSSH K

References

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[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"A cell plate-specific callose synthase and its interaction with phragmoplastin." Hong Z., Delauney A.J., Verma D.P.S. Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract] Cited for: GENE FAMILY AND NOMENCLATURE.
[3]	"An Arabidopsis callose synthase, GSL5, is required for wound and papillary callose formation." Jacobs A.K., Lipka V., Burton R.A., Panstruga R., Strizhov N., Schulze-Lefert P., Fincher G.B. Plant Cell 15:2503-2513(2003) [PubMed: 14555698] [Abstract] Cited for: FUNCTION.
[4]	"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility." Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E. Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL163527 Genomic DNA. Translation: CAB86938.1. Sequence problems.
IPI	IPI00530564.
PIR	T47792.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT48. Glycosyltransferase Family 48.
Genome annotation databases
GenomeReviews	Gene locus AT3G59100 in contig BA000014_GR.
Organism-specific databases
TAIR	At3g59100.
Family and domain databases
InterPro	IPR003440. Glyco_trans_48. [Graphical view]
Pfam	PF02364. Glucan_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALS6_ARATH

Accession

Primary (citable) accession number: Q9LYS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)