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Protein

Calcium-transporting ATPase 12, plasma membrane-type

Gene

ACA12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei453 – 45314-aspartylphosphate intermediateBy similarity
Metal bindingi751 – 7511MagnesiumBy similarity
Metal bindingi755 – 7551MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G63380-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.2.42. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 12, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 12
Gene namesi
Name:ACA12
Ordered Locus Names:At3g63380
ORF Names:MAA21_10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G63380.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 152152CytoplasmicSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Topological domaini174 – 19118LumenalSequence analysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence analysisAdd
BLAST
Topological domaini213 – 341129CytoplasmicSequence analysisAdd
BLAST
Transmembranei342 – 36120HelicalSequence analysisAdd
BLAST
Topological domaini362 – 39736LumenalSequence analysisAdd
BLAST
Transmembranei398 – 41518HelicalSequence analysisAdd
BLAST
Topological domaini416 – 806391CytoplasmicSequence analysisAdd
BLAST
Transmembranei807 – 82519HelicalSequence analysisAdd
BLAST
Topological domaini826 – 83611LumenalSequence analysisAdd
BLAST
Transmembranei837 – 85721HelicalSequence analysisAdd
BLAST
Topological domaini858 – 87720CytoplasmicSequence analysisAdd
BLAST
Transmembranei878 – 90023HelicalSequence analysisAdd
BLAST
Topological domaini901 – 9099LumenalSequence analysis
Transmembranei910 – 93021HelicalSequence analysisAdd
BLAST
Topological domaini931 – 94818CytoplasmicSequence analysisAdd
BLAST
Transmembranei949 – 97022HelicalSequence analysisAdd
BLAST
Topological domaini971 – 98010LumenalSequence analysis
Transmembranei981 – 100222HelicalSequence analysisAdd
BLAST
Topological domaini1003 – 10064CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Calcium-transporting ATPase 12, plasma membrane-typePRO_0000046418Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9LY77.
PRIDEiQ9LY77.

Expressioni

Gene expression databases

GenevisibleiQ9LY77. AT.

Interactioni

Protein-protein interaction databases

BioGridi10827. 19 interactions.
STRINGi3702.AT3G63380.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LY77.
SMRiQ9LY77. Positions 108-1004.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 3612Interaction with calmodulinCuratedAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LY77.
KOiK01537.
OMAiTHIDSSI.
OrthoDBiEOG093601Z7.
PhylomeDBiQ9LY77.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LY77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDLKEYDYS ALLLNLTTSS LNKAQRRWRF AYAAIYSMRA MLSLVKEIVP
60 70 80 90 100
ARIDPKTSDA SLSLSYTALE SGEGAKINSM PLSYVPAIDQ EQLVEIMKGK
110 120 130 140 150
DLPGIQALGG VEGVAASLRT NPTKGIHGNE QEVSRRRDLF GSNTYHKPPP
160 170 180 190 200
KGLLFFVYEA FKDLTILILL VCAIFSLGFG IKEHGIKEGW YEGGSIFVAV
210 220 230 240 250
FLVIVVSALS NFRQERQFDK LSKISNNIKV EVLRDSRRQH ISIFDVVVGD
260 270 280 290 300
VVFLKIGDQI PADGLFLEGH SLQVDESSMT GESDHLEVDH KDNPFLFSGT
310 320 330 340 350
KIVDGFAQML VVSVGMSTTW GQTMSSINQD SSERTPLQVR LDTLTSTIGK
360 370 380 390 400
IGLTVAALVL VVLLVRYFTG NTEKEGKREY NGSKTPVDTV VNSVVRIVAA
410 420 430 440 450
AVTIVVVAIP EGLPLAVTLT LAYSMKRMMS DQAMVRKLSA CETMGSATVI
460 470 480 490 500
CTDKTGTLTL NEMKVTKFWL GQESIHEDST KMISPDVLDL LYQGTGLNTT
510 520 530 540 550
GSVCVSDSGS TPEFSGSPTE KALLSWTVLN LGMDMESVKQ KHEVLRVETF
560 570 580 590 600
SSAKKRSGVL VRRKSDNTVH VHWKGAAEMV LAMCSHYYTS TGSVDLMDST
610 620 630 640 650
AKSRIQAIIQ GMAASSLRCI AFAHKIASND SVLEEDGLTL MGIVGLKDPC
660 670 680 690 700
RPGVSKAVET CKLAGVTIKM ITGDNVFTAK AIAFECGILD HNDKDEEDAV
710 720 730 740 750
VEGVQFRNYT DEERMQKVDK IRVMARSSPS DKLLMVKCLR LKGHVVAVTG
760 770 780 790 800
DGTNDAPALK EADIGLSMGI QGTEVAKESS DIVILDDNFA SVATVLKWGR
810 820 830 840 850
CVYNNIQKFI QFQLTVNVAA LVINFIAAIS AGEVPLTAVQ LLWVNLIMDT
860 870 880 890 900
LGALALATER PTNELLKRKP VGRTEALITN VMWRNLLVQS LYQIAVLLIL
910 920 930 940 950
QFKGMSIFSV RKEVKDTLIF NTFVLCQVFN EFNAREMEKK NVFKGLHRNR
960 970 980 990 1000
LFIGIIAITI VLQVIMVEFL KKFADTVRLN GWQWGTCIAL ASLSWPIGFF
1010 1020 1030
TKFIPVSETP FLSYFKNPRS LFKGSRSPSL KKP
Length:1,033
Mass (Da):113,707
Last modified:October 1, 2000 - v1
Checksum:iD0898F370970D451
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti239 – 2391Q → R in BAC41935 (PubMed:11910074).Curated
Sequence conflicti239 – 2391Q → R in AAO64912 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163818 Genomic DNA. Translation: CAB87791.1.
CP002686 Genomic DNA. Translation: AEE80473.1.
AK117260 mRNA. Translation: BAC41935.1.
BT005977 mRNA. Translation: AAO64912.1.
PIRiT49179.
RefSeqiNP_191897.1. NM_116203.2.
UniGeneiAt.26192.
At.33936.

Genome annotation databases

EnsemblPlantsiAT3G63380.1; AT3G63380.1; AT3G63380.
GeneIDi825513.
GrameneiAT3G63380.1; AT3G63380.1; AT3G63380.
KEGGiath:AT3G63380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163818 Genomic DNA. Translation: CAB87791.1.
CP002686 Genomic DNA. Translation: AEE80473.1.
AK117260 mRNA. Translation: BAC41935.1.
BT005977 mRNA. Translation: AAO64912.1.
PIRiT49179.
RefSeqiNP_191897.1. NM_116203.2.
UniGeneiAt.26192.
At.33936.

3D structure databases

ProteinModelPortaliQ9LY77.
SMRiQ9LY77. Positions 108-1004.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10827. 19 interactions.
STRINGi3702.AT3G63380.1.

Protein family/group databases

TCDBi3.A.3.2.42. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbiQ9LY77.
PRIDEiQ9LY77.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G63380.1; AT3G63380.1; AT3G63380.
GeneIDi825513.
GrameneiAT3G63380.1; AT3G63380.1; AT3G63380.
KEGGiath:AT3G63380.

Organism-specific databases

TAIRiAT3G63380.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiQ9LY77.
KOiK01537.
OMAiTHIDSSI.
OrthoDBiEOG093601Z7.
PhylomeDBiQ9LY77.

Enzyme and pathway databases

BioCyciARA:AT3G63380-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiQ9LY77.

Gene expression databases

GenevisibleiQ9LY77. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACA12_ARATH
AccessioniPrimary (citable) accession number: Q9LY77
Secondary accession number(s): Q8GZ18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.