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Q9LY51

- PLCD7_ARATH

UniProt

Q9LY51 - PLCD7_ARATH

Protein

Phosphoinositide phospholipase C 7

Gene

PLC7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei118 – 1181PROSITE-ProRule annotation
    Active sitei164 – 1641PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT3G55940-MONOMER.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 7 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC7
    Short name:
    AtPLC10
    Short name:
    AtPLC7
    Short name:
    PI-PLC7
    Gene namesi
    Name:PLC7
    Synonyms:PLC10
    Ordered Locus Names:At3g55940
    ORF Names:F27K19.120
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G55940.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Phosphoinositide phospholipase C 7PRO_0000324132Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei287 – 2871Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9LY51.
    PRIDEiQ9LY51.

    Expressioni

    Tissue specificityi

    Expressed in leaves, roots, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ9LY51.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G55940.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LY51.
    SMRiQ9LY51. Positions 25-581.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 10277EF-hand-likeAdd
    BLAST
    Domaini103 – 248146PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini323 – 439117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini442 – 549108C2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi258 – 31255Asp-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand-like domain.Curated
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ9LY51.
    KOiK05857.
    OMAiTIWHESH.
    PhylomeDBiQ9LY51.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9LY51-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSKQTYKVCF CFRRRYRHTV SVAPAEIKTL FDNYSDKGLM TTDLLLRFLI    50
    DVQKQDKATK EEAQDIVNAS SSLLHRNGLH LDAFFKYLFA VTNSPLSSLE 100
    VHQDMDAPLS HYFIYTGHNS YLTGNQLSSD CSELPIIEAL KKGVRVIELD 150
    IWPNSDEDGI DVLHGRTLTS PVELIKCLRA IREHAFDVSD YPVVVTLEDH 200
    LTPKLQAKVA EMVTDIFGEM LFTPPSGECL KEFPSPAFLK KRIMISTKPP 250
    KEYKAATDDD LVKKGRDLGD KEVWGREVPS FIRRDRSVDK NDSNGDDDDD 300
    DDDDDDDDDG DDKIKKNAPP EYKHLIAIEA GKPKGGITEC LKVDPDKVRR 350
    LSLSEEQLEK ASEKYAKQIV RFTQRNLLRV YPKGTRITSS NYNPLIAWSH 400
    GAQMVAFNMQ GLGRSLWVMQ GMFRGNGGCG YIKKPDLLLK SNAVFDPEAT 450
    LPVKTTLRVT IYMGEGWYYD FPHTHFDRYS PPDFYTRVGI AGVPADTVMK 500
    KTKTLEDNWI PAWDEVFEFP LTVPELALLR IEVHEYDMSE KDDFGGQICL 550
    PVWELRQGIR AVPLRNQDGV KCRSVKLLVR LEFV 584

    Note: No experimental confirmation available.

    Length:584
    Mass (Da):66,462
    Last modified:October 1, 2000 - v1
    Checksum:i16A8C5A402A8BA05
    GO
    Isoform 2 (identifier: Q9LY51-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         212-214: MVT → GNV
         215-584: Missing.

    Note: No experimental confirmation available. Potentially inactive.

    Show »
    Length:214
    Mass (Da):24,243
    Checksum:i64CD6BEAD25205DB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei212 – 2143MVT → GNV in isoform 2. 1 PublicationVSP_032149
    Alternative sequencei215 – 584370Missing in isoform 2. 1 PublicationVSP_032150Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL163832 Genomic DNA. Translation: CAB87848.1.
    CP002686 Genomic DNA. Translation: AEE79459.1.
    PIRiT49206.
    RefSeqiNP_191153.1. NM_115452.1. [Q9LY51-1]
    UniGeneiAt.53946.

    Genome annotation databases

    EnsemblPlantsiAT3G55940.1; AT3G55940.1; AT3G55940. [Q9LY51-1]
    GeneIDi824760.
    KEGGiath:AT3G55940.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL163832 Genomic DNA. Translation: CAB87848.1 .
    CP002686 Genomic DNA. Translation: AEE79459.1 .
    PIRi T49206.
    RefSeqi NP_191153.1. NM_115452.1. [Q9LY51-1 ]
    UniGenei At.53946.

    3D structure databases

    ProteinModelPortali Q9LY51.
    SMRi Q9LY51. Positions 25-581.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G55940.1-P.

    Proteomic databases

    PaxDbi Q9LY51.
    PRIDEi Q9LY51.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G55940.1 ; AT3G55940.1 ; AT3G55940 . [Q9LY51-1 ]
    GeneIDi 824760.
    KEGGi ath:AT3G55940.

    Organism-specific databases

    TAIRi AT3G55940.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q9LY51.
    KOi K05857.
    OMAi TIWHESH.
    PhylomeDBi Q9LY51.

    Enzyme and pathway databases

    BioCyci ARA:AT3G55940-MONOMER.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    Genevestigatori Q9LY51.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    4. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. La-0.
    6. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
      Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
      Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPLCD7_ARATH
    AccessioniPrimary (citable) accession number: Q9LY51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3