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Q9LY51

- PLCD7_ARATH

UniProt

Q9LY51 - PLCD7_ARATH

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Protein

Phosphoinositide phospholipase C 7

Gene

PLC7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.By similarity

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei118 – 1181PROSITE-ProRule annotation
Active sitei164 – 1641PROSITE-ProRule annotation

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G55940-MONOMER.
ReactomeiREACT_185773. Role of phospholipids in phagocytosis.
REACT_187614. Synthesis of IP3 and IP4 in the cytosol.
REACT_187625. Signaling by FGFR1 fusion mutants.
REACT_230384. Role of second messengers in netrin-1 signaling.
REACT_231497. GPVI-mediated activation cascade.
REACT_234578. Acetylcholine regulates insulin secretion.
REACT_247278. ISG15 antiviral mechanism.
REACT_248872. DAG and IP3 signaling.
REACT_258042. G alpha (q) signalling events.
REACT_260006. Generation of second messenger molecules.
REACT_260557. VEGFR2 mediated cell proliferation.
REACT_261531. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoinositide phospholipase C 7 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase PLC7
Short name:
AtPLC10
Short name:
AtPLC7
Short name:
PI-PLC7
Gene namesi
Name:PLC7
Synonyms:PLC10
Ordered Locus Names:At3g55940
ORF Names:F27K19.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G55940.

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Phosphoinositide phospholipase C 7PRO_0000324132Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei287 – 2871Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LY51.
PRIDEiQ9LY51.

Expressioni

Tissue specificityi

Expressed in leaves, roots, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ9LY51.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G55940.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LY51.
SMRiQ9LY51. Positions 25-581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 10277EF-hand-likeAdd
BLAST
Domaini103 – 248146PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini323 – 439117PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini442 – 549108C2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi258 – 31255Asp-richAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 EF-hand-like domain.Curated
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000244119.
InParanoidiQ9LY51.
KOiK05857.
OMAiTIWHESH.
PhylomeDBiQ9LY51.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9LY51-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSKQTYKVCF CFRRRYRHTV SVAPAEIKTL FDNYSDKGLM TTDLLLRFLI
60 70 80 90 100
DVQKQDKATK EEAQDIVNAS SSLLHRNGLH LDAFFKYLFA VTNSPLSSLE
110 120 130 140 150
VHQDMDAPLS HYFIYTGHNS YLTGNQLSSD CSELPIIEAL KKGVRVIELD
160 170 180 190 200
IWPNSDEDGI DVLHGRTLTS PVELIKCLRA IREHAFDVSD YPVVVTLEDH
210 220 230 240 250
LTPKLQAKVA EMVTDIFGEM LFTPPSGECL KEFPSPAFLK KRIMISTKPP
260 270 280 290 300
KEYKAATDDD LVKKGRDLGD KEVWGREVPS FIRRDRSVDK NDSNGDDDDD
310 320 330 340 350
DDDDDDDDDG DDKIKKNAPP EYKHLIAIEA GKPKGGITEC LKVDPDKVRR
360 370 380 390 400
LSLSEEQLEK ASEKYAKQIV RFTQRNLLRV YPKGTRITSS NYNPLIAWSH
410 420 430 440 450
GAQMVAFNMQ GLGRSLWVMQ GMFRGNGGCG YIKKPDLLLK SNAVFDPEAT
460 470 480 490 500
LPVKTTLRVT IYMGEGWYYD FPHTHFDRYS PPDFYTRVGI AGVPADTVMK
510 520 530 540 550
KTKTLEDNWI PAWDEVFEFP LTVPELALLR IEVHEYDMSE KDDFGGQICL
560 570 580
PVWELRQGIR AVPLRNQDGV KCRSVKLLVR LEFV

Note: No experimental confirmation available.

Length:584
Mass (Da):66,462
Last modified:October 1, 2000 - v1
Checksum:i16A8C5A402A8BA05
GO
Isoform 2 (identifier: Q9LY51-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-214: MVT → GNV
     215-584: Missing.

Note: No experimental confirmation available. Potentially inactive.

Show »
Length:214
Mass (Da):24,243
Checksum:i64CD6BEAD25205DB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei212 – 2143MVT → GNV in isoform 2. 1 PublicationVSP_032149
Alternative sequencei215 – 584370Missing in isoform 2. 1 PublicationVSP_032150Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163832 Genomic DNA. Translation: CAB87848.1.
CP002686 Genomic DNA. Translation: AEE79459.1.
PIRiT49206.
RefSeqiNP_191153.1. NM_115452.1. [Q9LY51-1]
UniGeneiAt.53946.

Genome annotation databases

EnsemblPlantsiAT3G55940.1; AT3G55940.1; AT3G55940. [Q9LY51-1]
GeneIDi824760.
KEGGiath:AT3G55940.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163832 Genomic DNA. Translation: CAB87848.1 .
CP002686 Genomic DNA. Translation: AEE79459.1 .
PIRi T49206.
RefSeqi NP_191153.1. NM_115452.1. [Q9LY51-1 ]
UniGenei At.53946.

3D structure databases

ProteinModelPortali Q9LY51.
SMRi Q9LY51. Positions 25-581.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G55940.1-P.

Proteomic databases

PaxDbi Q9LY51.
PRIDEi Q9LY51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G55940.1 ; AT3G55940.1 ; AT3G55940 . [Q9LY51-1 ]
GeneIDi 824760.
KEGGi ath:AT3G55940.

Organism-specific databases

TAIRi AT3G55940.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000244119.
InParanoidi Q9LY51.
KOi K05857.
OMAi TIWHESH.
PhylomeDBi Q9LY51.

Enzyme and pathway databases

BioCyci ARA:AT3G55940-MONOMER.
Reactomei REACT_185773. Role of phospholipids in phagocytosis.
REACT_187614. Synthesis of IP3 and IP4 in the cytosol.
REACT_187625. Signaling by FGFR1 fusion mutants.
REACT_230384. Role of second messengers in netrin-1 signaling.
REACT_231497. GPVI-mediated activation cascade.
REACT_234578. Acetylcholine regulates insulin secretion.
REACT_247278. ISG15 antiviral mechanism.
REACT_248872. DAG and IP3 signaling.
REACT_258042. G alpha (q) signalling events.
REACT_260006. Generation of second messenger molecules.
REACT_260557. VEGFR2 mediated cell proliferation.
REACT_261531. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.

Gene expression databases

Genevestigatori Q9LY51.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
    Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
    New Phytol. 162:643-654(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  4. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-287, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. La-0.
  6. "Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database."
    Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.
    Plant Cell 16:2394-2405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPLCD7_ARATH
AccessioniPrimary (citable) accession number: Q9LY51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3