Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATPase 7, plasma membrane-type

Gene

AHA7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses (By similarity).By similarity

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 33214-aspartylphosphate intermediateBy similarity
Metal bindingi588 – 5881MagnesiumBy similarity
Metal bindingi592 – 5921MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G60330-MONOMER.
ARA:GQT-2805-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 7, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 7
Gene namesi
Name:AHA7
Ordered Locus Names:At3g60330
ORF Names:F27H5_120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G60330.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6464CytoplasmicSequence analysisAdd
BLAST
Transmembranei65 – 8420Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini85 – 9612ExtracellularSequence analysisAdd
BLAST
Transmembranei97 – 11721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini118 – 246129CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26721Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini268 – 2769ExtracellularSequence analysis
Transmembranei277 – 29418Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini295 – 643349CytoplasmicSequence analysisAdd
BLAST
Transmembranei644 – 66522Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini666 – 6705ExtracellularSequence analysis
Transmembranei671 – 69323Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini694 – 70916CytoplasmicSequence analysisAdd
BLAST
Transmembranei710 – 73021Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini731 – 76434ExtracellularSequence analysisAdd
BLAST
Transmembranei765 – 78521Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini786 – 79712CytoplasmicSequence analysisAdd
BLAST
Transmembranei798 – 81821Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini819 – 8268ExtracellularSequence analysis
Transmembranei827 – 84721Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini848 – 961114CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961ATPase 7, plasma membrane-typePRO_0000046280Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei894 – 8941PhosphothreonineBy similarity
Modified residuei910 – 9101PhosphoserineBy similarity
Modified residuei942 – 9421PhosphoserineBy similarity
Modified residuei960 – 9601PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LY32.
PRIDEiQ9LY32.

Expressioni

Gene expression databases

GenevisibleiQ9LY32. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-960. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).By similarity

Protein-protein interaction databases

BioGridi10518. 4 interactions.
STRINGi3702.AT3G60330.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LY32.
SMRiQ9LY32. Positions 15-856, 913-952.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni959 – 9613Interaction with 14-3-3 proteinsBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9LY32.
KOiK01535.
OMAiWIFNIVT.
PhylomeDBiQ9LY32.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LY32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIEALKAI TTESIDLENV PVEEVFQHLK CTKEGLTSNE VQERLTLFGY
60 70 80 90 100
NKLEEKKESK ILKFLGFMWN PLSWVMEAAA LMAIGLAHGG GKPADYHDFV
110 120 130 140 150
GIVVLLLINS TISFVEENNA GNAAAALMAQ LAPKAKAVRD GKWNEIDAAE
160 170 180 190 200
LVPGDIVSIK LGDIIPADAR LLEGDPLKID QATLTGESLP VTKNPGASVY
210 220 230 240 250
SGSTCKQGEI EAVVIATGVH TFFGKAAHLV DSTTHVGHFQ KVLTAIGNFC
260 270 280 290 300
ICSIAVGMAI EIVVIYGLQK RGYRVGIDNL LVLLIGGIPI AMPTVLSVTM
310 320 330 340 350
AIGAHRLAQQ GAITKRMTAI EEMAGMDVLC SDKTGTLTLN KLSVDKNLIE
360 370 380 390 400
VFKRGIDRDM AVLMAARAAR LENQDAIDTA IVSMLSDPKE ARAGIKELHF
410 420 430 440 450
LPFSPANRRT ALTYLDGEGK MHRVSKGAPE EILDMAHNKL EIKEKVHATI
460 470 480 490 500
DKFAERGLRS LGLAYQEVPD GDVKGEGGPW DFVALLPLFD PPRHDSAQTI
510 520 530 540 550
ERALHLGVSV KMITGDQLAI AKETGRRLGM GTNMYPSSSL LSDNNTEGVS
560 570 580 590 600
VDELIENADG FAGVFPEHKY EIVKRLQSRK HICGMTGDGV NDAPALKKAD
610 620 630 640 650
IGIAVDDATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV
660 670 680 690 700
SITIRIVMGF MLLCVFWEFD FPPFMVLVIA ILNDGTIMTI SKDRVKPSPT
710 720 730 740 750
PDCWKLKEIF ATGVVLGAYL AIMTVVFFWA AYETNFFHNI FHVRNFNQHH
760 770 780 790 800
FKMKDKKVAA HLNEQMASAV YLQVSTISQA LIFVTRSRSW SFVERPGFLL
810 820 830 840 850
VIAFLIAQLV ASVISAMANW PFAGIRSIGW GWTGVIWIFN IVTYMLLDPI
860 870 880 890 900
KFLVRYALSG KSWDRMVEGR TALTGKKNFG QEERMAAWAT EKRTQHGLET
910 920 930 940 950
GQKPVYERNS ATELNNMAEE AKRRAEIARM RELQTLKGKV ESAAKLKGYD
960
LEDPNSNNYT I
Length:961
Mass (Da):105,520
Last modified:October 1, 2000 - v1
Checksum:iBF8F9C5BC234A4F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163852 Genomic DNA. Translation: CAB87870.1.
CP002686 Genomic DNA. Translation: AEE80046.1.
CP002686 Genomic DNA. Translation: AEE80047.1.
PIRiT49228.
RefSeqiNP_001190141.1. NM_001203212.1.
NP_191592.5. NM_115897.5.
UniGeneiAt.54016.

Genome annotation databases

EnsemblPlantsiAT3G60330.1; AT3G60330.1; AT3G60330.
AT3G60330.2; AT3G60330.2; AT3G60330.
GeneIDi825204.
GrameneiAT3G60330.1; AT3G60330.1; AT3G60330.
AT3G60330.2; AT3G60330.2; AT3G60330.
KEGGiath:AT3G60330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163852 Genomic DNA. Translation: CAB87870.1.
CP002686 Genomic DNA. Translation: AEE80046.1.
CP002686 Genomic DNA. Translation: AEE80047.1.
PIRiT49228.
RefSeqiNP_001190141.1. NM_001203212.1.
NP_191592.5. NM_115897.5.
UniGeneiAt.54016.

3D structure databases

ProteinModelPortaliQ9LY32.
SMRiQ9LY32. Positions 15-856, 913-952.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10518. 4 interactions.
STRINGi3702.AT3G60330.1.

Proteomic databases

PaxDbiQ9LY32.
PRIDEiQ9LY32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G60330.1; AT3G60330.1; AT3G60330.
AT3G60330.2; AT3G60330.2; AT3G60330.
GeneIDi825204.
GrameneiAT3G60330.1; AT3G60330.1; AT3G60330.
AT3G60330.2; AT3G60330.2; AT3G60330.
KEGGiath:AT3G60330.

Organism-specific databases

TAIRiAT3G60330.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ9LY32.
KOiK01535.
OMAiWIFNIVT.
PhylomeDBiQ9LY32.

Enzyme and pathway databases

BioCyciARA:AT3G60330-MONOMER.
ARA:GQT-2805-MONOMER.

Miscellaneous databases

PROiQ9LY32.

Gene expression databases

GenevisibleiQ9LY32. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPMA7_ARATH
AccessioniPrimary (citable) accession number: Q9LY32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: April 13, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.