Reviewed, UniProtKB/Swiss-Prot Q9LY19 (PME48_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectinesterase 48
      Short name=PE 48
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 48
      Short name=AtPME48

Gene names

Name:	PME48
Synonyms:	ARATH48
Ordered Locus Names:	At5g07410
ORF Names:	T2I1.120

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in mature pollen grains in the anthers and on the stigma. Found in pollen tubes within the style. Ref.5
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 361	338	Probable pectinesterase 48		PRO_0000371696
Sites
	Active site	197	1	Proton donor By similarity
	Active site	218	1	Nucleophile By similarity
	Binding site	174	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity
	Site	196	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	48	1	A → V in AAM65347. Ref.2
	Sequence conflict	100	1	H → P in AAM65347. Ref.2
	Sequence conflict	254	1	K → N in AAM65347. Ref.2
	Sequence conflict	270	1	G → V in AAM65347. Ref.2
	Sequence conflict	342	1	C → R in AAM65347. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9LY19-1 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: C0663F0A5FAB2D3E	FASTA	361	39,337
	10 20 30 40 50 60 MRYTNVSILL GMLVIFVSPM VFADDLTPIP EGKPQVVQWF NTHVGPLAQR KGLDPALVAA 70 80 90 100 110 120 EAAPRIINVN PKGGEFKTLT DAIKSVPAGN TKRVIIKMAH GEYREKVTID RNKPFITLMG 130 140 150 160 170 180 QPNAMPVITY DGTAAKYGTV DSASLIILSD YFMAVNIVVK NTAPAPDGKT KGAQALSMRI 190 200 210 220 230 240 SGNFAAFYNC KFYGFQDTIC DDTGNHFFKD CYVEGTFDFI FGSGTSMYLG TQLHVVGDGI 250 260 270 280 290 300 RVIAAHAGKS AEEKSGYSFV HCKVTGTGGG IYLGRAWMSH PKVVYAYTEM TSVVNPTGWQ 310 320 330 340 350 360 ENKTPAHDKT VFYGEYKCSG PGSHKAKRVP FTQDIDDKEA NCFLSLGYIQ GSKWLLPPPA L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 257-361. Strain: cv. Columbia.
[4]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL163912 Genomic DNA. Translation: CAB87930.1. Different initiation. AY087811 mRNA. Translation: AAM65347.1. Different initiation. AK221986 mRNA. Translation: BAD94545.1. Different initiation.
IPI	IPI00545321.
PIR	T49880.
RefSeq	NP_568181.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9LY19.
Genome annotation databases
GeneID	830632.
GenomeReviews	Gene locus AT5G07410 in contig BA000015_GR.
NMPDR	fig|3702.1.peg.22881.
Organism-specific databases
GeneFarm	502. 8.
TAIR	At5g07410.
Gene expression databases
Genevestigator	Q9LY19.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME48_ARATH

Accession

Primary (citable) accession number: Q9LY19
Secondary accession number(s): Q56WP9, Q8LAH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	October 13, 2009
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)