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Protein

Dicarboxylate transporter 1, chloroplastic

Gene

DIT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

2-oxoglutarate/malate translocator involved with DIT2-1 in primary ammonia assimilation and in the re-assimilation of ammonia generated by the photorespiratory pathway. Imports 2-oxoglutarate into plastids as precursor for ammonia assimilation. 2-oxoglutarate is converted to glutamate, the end product of ammonia assimilation, which is exported to the cytosol by DIT2-1.2 Publications

Kineticsi

  1. KM=0.7 mM for malate1 Publication
  2. KM=0.042 mM for oxaloacetate1 Publication

    GO - Molecular functioni

    • alpha-ketoglutarate transmembrane transporter activity Source: UniProtKB
    • malate transmembrane transporter activity Source: UniProtKB
    • oxaloacetate transmembrane transporter activity Source: UniProtKB

    GO - Biological processi

    • alpha-ketoglutarate transport Source: UniProtKB
    • ammonia assimilation cycle Source: UniProtKB
    • malate transmembrane transport Source: UniProtKB
    • oxaloacetate transport Source: UniProtKB
    • response to nematode Source: TAIR
    • sodium ion transport Source: InterPro

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    SABIO-RKiQ9LXV3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dicarboxylate transporter 1, chloroplastic
    Alternative name(s):
    2-oxoglutarate/malate translocator 1
    Short name:
    AtpOMT1
    Gene namesi
    Name:DIT1
    Ordered Locus Names:At5g12860
    ORF Names:T24H18.30
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G12860.
    TAIRilocus:2182270. AT5G12860.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei90 – 110HelicalSequence analysisAdd BLAST21
    Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
    Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
    Transmembranei229 – 249HelicalSequence analysisAdd BLAST21
    Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
    Transmembranei305 – 325HelicalSequence analysisAdd BLAST21
    Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
    Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
    Transmembranei411 – 431HelicalSequence analysisAdd BLAST21
    Transmembranei438 – 458HelicalSequence analysisAdd BLAST21
    Transmembranei477 – 497HelicalSequence analysisAdd BLAST21
    Transmembranei531 – 551HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Plastid inner membrane

    Pathology & Biotechi

    Disruption phenotypei

    Reduced growth and amino acid levels, but growth inhibition is prevented when mutant plants are grown under non-photorespiratory high CO2 conditions.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 69ChloroplastSequence analysisAdd BLAST69
    ChainiPRO_000041918370 – 557Dicarboxylate transporter 1, chloroplasticAdd BLAST488

    Proteomic databases

    PaxDbiQ9LXV3.
    PRIDEiQ9LXV3.

    PTM databases

    SwissPalmiQ9LXV3.

    Expressioni

    Tissue specificityi

    Expressed in roots, rosette and cauline leaves, stems, flowers and siliques.2 Publications

    Inductioni

    By nitrate after two days of nitrogen deprivation and re-illumination after three days of dark adaptation.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9LXV3. baseline and differential.
    GenevisibleiQ9LXV3. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi16404. 35 interactors.
    STRINGi3702.AT5G12860.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXV3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IFDX. Eukaryota.
    COG0471. LUCA.
    HOGENOMiHOG000117912.
    InParanoidiQ9LXV3.
    OMAiNPNAWLY.
    OrthoDBiEOG093607UA.
    PhylomeDBiQ9LXV3.

    Family and domain databases

    InterProiView protein in InterPro
    IPR030676. CitT-rel.
    IPR001898. SLC13A/DASS.
    PANTHERiPTHR42826. PTHR42826. 1 hit.
    PfamiView protein in Pfam
    PF00939. Na_sulph_symp. 1 hit.
    TIGRFAMsiTIGR00785. dass. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

    Note: A number of isoforms are produced. According to EST sequences.
    Isoform 1 (identifier: Q9LXV3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MASLALSGSC SLAFPLKSRS LSLPRPPSSS LNLTKPLRSL DSRFSLLKSP
    60 70 80 90 100
    LPVSLRRRSS TLVKASSTVA SASSSPTPPL VPAPVPWQGA AIKPLLASIA
    110 120 130 140 150
    TGLILWFVPV PEGVTRNAWQ LLAIFLATIV GIITQPLPLG AVALMGLGAS
    160 170 180 190 200
    VLTKTLTFAA AFSAFGDPIP WLIALAFFFA RGFIKTGLGN RVAYQFVRLF
    210 220 230 240 250
    GSSSLGLGYS LVFSEALLAP AIPSVSARAG GIFLPLVKSL CVACGSNVGD
    260 270 280 290 300
    GTEHRLGSWL MLTCFQTSVI SSSMFLTAMA ANPLSANLAF NTIKQTIGWT
    310 320 330 340 350
    DWAKAAIVPG LVSLIVVPFL LYLIYPPTVK SSPDAPKLAQ EKLDKMGPMS
    360 370 380 390 400
    KNELIMAATL FLTVGLWIFG AKLGVDAVTA AILGLSVLLV TGVVTWKECL
    410 420 430 440 450
    AESVAWDTLT WFAALIAMAG YLNKYGLIEW FSQTVVKFVG GLGLSWQLSF
    460 470 480 490 500
    GILVLLYFYT HYFFASGAAH IGAMFTAFLS VSTALGTPPY FAALVLAFLS
    510 520 530 540 550
    NLMGGLTHYG IGSAPIFYGA NYVPLAKWWG YGFLISIVNI LIWLGVGGAW

    WKFIGLW
    Length:557
    Mass (Da):59,213
    Last modified:October 1, 2000 - v1
    Checksum:i13AE5458E9C5337C
    GO

    Sequence cautioni

    The sequence AAK43871 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti496L → H in AAK43871 (PubMed:14593172).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL353013 Genomic DNA. Translation: CAB88250.1.
    CP002688 Genomic DNA. Translation: AED91822.1.
    AF370494 mRNA. Translation: AAK43871.1. Different initiation.
    PIRiT49900.
    RefSeqiNP_568283.2. NM_121289.4. [Q9LXV3-1]
    UniGeneiAt.5282.

    Genome annotation databases

    EnsemblPlantsiAT5G12860.1; AT5G12860.1; AT5G12860. [Q9LXV3-1]
    GeneIDi831126.
    GrameneiAT5G12860.1; AT5G12860.1; AT5G12860.
    KEGGiath:AT5G12860.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiDIT1_ARATH
    AccessioniPrimary (citable) accession number: Q9LXV3
    Secondary accession number(s): Q93Y83
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: October 1, 2000
    Last modified: August 30, 2017
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families