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Reviewed, UniProtKB/Swiss-Prot Q9LXT9 (CALS3_ARATH)

Last modified June 16, 2009. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Callose synthase 3
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 12
Gene names
Name: CALS3
Synonyms: GSL12
Ordered Locus Names: At5g13000
ORF Names: T24H18.170
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length1964 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence CAB88264.1 differs from that shown. Reason: Erroneous gene model prediction.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19641964Callose synthase 3
PRO_0000334575

Regions

Topological domain1 – 488488Cytoplasmic Potential
Transmembrane489 – 50921 Potential
Topological domain510 – 52112Extracellular Potential
Transmembrane522 – 54221 Potential
Topological domain543 – 55816Cytoplasmic Potential
Transmembrane559 – 57921 Potential
Topological domain580 – 60425Extracellular Potential
Transmembrane605 – 62521 Potential
Topological domain626 – 66035Cytoplasmic Potential
Transmembrane661 – 68121 Potential
Topological domain682 – 71736Extracellular Potential
Transmembrane718 – 73821 Potential
Topological domain739 – 1526788Cytoplasmic Potential
Transmembrane1527 – 154721 Potential
Topological domain1548 – 157528Extracellular Potential
Transmembrane1576 – 159621 Potential
Topological domain1597 – 160610Cytoplasmic Potential
Transmembrane1607 – 162721 Potential
Topological domain1628 – 167043Extracellular Potential
Transmembrane1671 – 169121 Potential
Topological domain1692 – 16976Cytoplasmic Potential
Transmembrane1698 – 171821 Potential
Topological domain1719 – 177052Extracellular Potential
Transmembrane1771 – 179121 Potential
Topological domain1792 – 180110Cytoplasmic Potential
Transmembrane1802 – 182221 Potential
Topological domain1823 – 184220Extracellular Potential
Transmembrane1843 – 186321 Potential
Topological domain1864 – 18652Cytoplasmic Potential
Transmembrane1866 – 188621 Potential
Topological domain1887 – 190822Extracellular Potential
Transmembrane1909 – 192921 Potential
Topological domain1930 – 196435Cytoplasmic Potential
Compositional bias230 – 2334Poly-Lys

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Sequences

Sequence LengthMass (Da)Tools
Q9LXT9-1 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: C60CCE802F276045

FASTA1,964227,292
        10         20         30         40         50         60 
MSATRGGPDQ GPSQPQQRRI IRTQTAGNLG ESFDSEVVPS SLVEIAPILR VANEVESSNP 

        70         80         90        100        110        120 
RVAYLCRFYA FEKAHRLDPT SSGRGVRQFK TALLQRLERE HDPTLMGRVK KSDAREMQSF 

       130        140        150        160        170        180 
YQHYYKKYIQ ALHNAADKAD RAQLTKAYQT ANVLFEVLKA VNLTQSIEVD REILEAQDKV 

       190        200        210        220        230        240 
AEKTQLYVPY NILPLDPDSA NQAIMRYPEI QAAVLALRNT RGLPWPEGHK KKKDEDMLDW 

       250        260        270        280        290        300 
LQEMFGFQKD NVANQREHLI LLLANVHIRQ FPKPDQQPKL DDQALTEVMK KLFKNYKKWC 

       310        320        330        340        350        360 
KYLGRKSSLW LPTIQQEMQQ RKLLYMALYL LIWGEAANLR FMPECLCYIY HHMAFELYGM 

       370        380        390        400        410        420 
LAGNVSPMTG ENVKPAYGGE EDAFLRKVVT PIYEVIQMEA QRSKKGKSKH SQWRNYDDLN 

       430        440        450        460        470        480 
EYFWSVDCFR LGWPMRADAD FFCLPVAVPN TEKDGDNSKP IVARDRWVGK VNFVEIRSFW 

       490        500        510        520        530        540 
HVFRSFDRMW SFYILCLQAM IIMAWDGGQP SSVFGADVFK KVLSVFITAA IMKLGQAVLD 

       550        560        570        580        590        600 
VILNFKAHQS MTLHVKLRYI LKVFSAAAWV IILPVTYAYS WKDPPAFART IKSWFGSAMH 

       610        620        630        640        650        660 
SPSLFIIAVV SYLSPNMLAG VMFLFPLLRR FLERSNYRIV MLMMWWSQPR LYVGRGMHES 

       670        680        690        700        710        720 
AFSLFKYTMF WVLLIATKLA FSYYIEIRPL VAPTQAIMKA RVTNFQWHEF FPRAKNNIGV 

       730        740        750        760        770        780 
VIALWAPIIL VYFMDSQIWY AIFSTLFGGI YGAFRRLGEI RTLGMLRSRF ESLPGAFNDR 

       790        800        810        820        830        840 
LIPDGKNQQK KKGIRATLSH NFTEDKVPVN KEKEAARFAQ LWNTIISSFR EEDLISDREM 

       850        860        870        880        890        900 
DLLLVPYWAD RDLDLIQWPP FLLASKIPIA LDMAKDSNGK DRELKKRIES DTYMKCAVRE 

       910        920        930        940        950        960 
CYASFKNIIK FVVQGNREKE VIEIIFAEVD KHIDTGDLIQ EYKMSALPSL YDHFVKLIKY 

       970        980        990       1000       1010       1020 
LLDNKEEDRD HVVILFQDML EVVTRDIMME DYNISRLATF YRTAMACHSS HGGTWHGGMI 

      1030       1040       1050       1060       1070       1080 
PLEQQYQLFA SSGAIRFPIE PVTEAWKEKI KRIYLLLTTK ESAMDVPSNL EARRRISFFS 

      1090       1100       1110       1120       1130       1140 
NSLFMDMPMA PKVRNMLSFS VLTPYYTEEV LFSLRDLETP NEDGVSILFY LQKIFPDEWN 

      1150       1160       1170       1180       1190       1200 
NFLERVKCLS EEELKESDEL EEELRLWASY RGQTLTRTVR GMMYYRKALE LQAFLDMAMH 

      1210       1220       1230       1240       1250       1260 
EDLMEGYKAV ELNSENNSRG ERSLWAQCQA VADMKFTYVV SCQQYGIHKR SGDPRAQDIL 

      1270       1280       1290       1300       1310       1320 
RLMTRYPSLR VAYIDEVEEP VKDKSKKGNQ KVYYSVLVKV PKSTDHSTLA QNLDQVIYRI 

      1330       1340       1350       1360       1370       1380 
RLPGPAILGE GKPENQNHAI IFSRGEGLQT IDMNQDNYME EALKMRNLLQ EFLTKHDGVR 

      1390       1400       1410       1420       1430       1440 
HPSILGLREH IFTGSVSSLA WFMSNQETSF VTIGQRLLAN PLRVRFHYGH PDVFDRLFHL 

      1450       1460       1470       1480       1490       1500 
TRGGVSKASK VINLSEDIFA GFNSTLREGN VTHHEYIQVG KGRDVGLNQI SMFEAKIANG 

      1510       1520       1530       1540       1550       1560 
NGEQTLSRDI YRLGHRFDFF RMMSCYFTTV GFYFSTLITV LTVYIFLYGR LYLVLSGLEQ 

      1570       1580       1590       1600       1610       1620 
GLSTQKGIRD NTPLQIALAS QSFVQIGFLM ALPMLMEIGL ERGFRTALSE FVLMQLQLAP 

      1630       1640       1650       1660       1670       1680 
VFFTFSLGTK THYYGRTLLH GGAKYRSTGR GFVVFHAKFA DNYRLYSRSH FVKGLEMMLL 

      1690       1700       1710       1720       1730       1740 
LVVYQIFGSA YRGVLAYLLI TISMWFMVGT WLFAPFLFNP SGFEWQKIVD DWTDWNKWIN 

      1750       1760       1770       1780       1790       1800 
NIGGIGVPAE KSWESWWEEE QEHLRYSGKR GIVVEILLAL RFFIYQYGLV YHLTITEKTK 

      1810       1820       1830       1840       1850       1860 
NFLVYGVSWL VIFLILFVMK TVSVGRRRFS ASFQLMFRLI KGLIFMTFIA IIVILITLAH 

      1870       1880       1890       1900       1910       1920 
MTIQDIIVCI LAFMPTGWGM LLIAQACKPV VHRAGFWGSV RTLARGYEIV MGLLLFTPVA 

      1930       1940       1950       1960 
FLAWFPFVSE FQTRMLFNQA FSRGLQISRI LGGHRKDRSS RNKE

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References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[3]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.

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Cross-references

Sequence databases

AL353013 Genomic DNA. Translation: CAB88264.1. Sequence problems.
IPIIPI00533855.
PIRT49914.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9LXT9.

Genome annotation databases

GenomeReviewsGene locus AT5G13000 in contig BA000015_GR.

Organism-specific databases

TAIRAt5g13000.

Family and domain databases

InterProIPR003440. Glyco_trans_48.
[Graphical view]
PfamPF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCALS3_ARATH
AccessionPrimary (citable) accession number: Q9LXT9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 16, 2009
This is version 31 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents