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Q9LXT9 (CALS3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Callose synthase 3
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 12
Gene names
Name:CALS3
Synonyms:GSL12
Ordered Locus Names:At5g13000
ORF Names:T24H18.170
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1955 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in callose synthesis at the forming cell plate during cytokinesis. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals By similarity.

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence CAB88264.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LXT9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19551955Callose synthase 3
PRO_0000334575

Regions

Topological domain1 – 488488Cytoplasmic Potential
Transmembrane489 – 50921Helical; Potential
Topological domain510 – 52112Extracellular Potential
Transmembrane522 – 54221Helical; Potential
Topological domain543 – 55816Cytoplasmic Potential
Transmembrane559 – 57921Helical; Potential
Topological domain580 – 60425Extracellular Potential
Transmembrane605 – 62521Helical; Potential
Topological domain626 – 66035Cytoplasmic Potential
Transmembrane661 – 68121Helical; Potential
Topological domain682 – 71736Extracellular Potential
Transmembrane718 – 73821Helical; Potential
Topological domain739 – 1517779Cytoplasmic Potential
Transmembrane1518 – 153821Helical; Potential
Topological domain1539 – 156628Extracellular Potential
Transmembrane1567 – 158721Helical; Potential
Topological domain1588 – 159710Cytoplasmic Potential
Transmembrane1598 – 161821Helical; Potential
Topological domain1619 – 166143Extracellular Potential
Transmembrane1662 – 168221Helical; Potential
Topological domain1683 – 16886Cytoplasmic Potential
Transmembrane1689 – 170921Helical; Potential
Topological domain1710 – 176152Extracellular Potential
Transmembrane1762 – 178221Helical; Potential
Topological domain1783 – 179210Cytoplasmic Potential
Transmembrane1793 – 181321Helical; Potential
Topological domain1814 – 183320Extracellular Potential
Transmembrane1834 – 185421Helical; Potential
Topological domain1855 – 18562Cytoplasmic Potential
Transmembrane1857 – 187721Helical; Potential
Topological domain1878 – 189922Extracellular Potential
Transmembrane1900 – 192021Helical; Potential
Topological domain1921 – 195535Cytoplasmic Potential
Compositional bias230 – 2334Poly-Lys

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 16, 2011. Version 3.
Checksum: BE0E685D1F0EE13E

FASTA1,955226,184
        10         20         30         40         50         60 
MSATRGGPDQ GPSQPQQRRI IRTQTAGNLG ESFDSEVVPS SLVEIAPILR VANEVESSNP 

        70         80         90        100        110        120 
RVAYLCRFYA FEKAHRLDPT SSGRGVRQFK TALLQRLERE HDPTLMGRVK KSDAREMQSF 

       130        140        150        160        170        180 
YQHYYKKYIQ ALHNAADKAD RAQLTKAYQT ANVLFEVLKA VNLTQSIEVD REILEAQDKV 

       190        200        210        220        230        240 
AEKTQLYVPY NILPLDPDSA NQAIMRYPEI QAAVLALRNT RGLPWPEGHK KKKDEDMLDW 

       250        260        270        280        290        300 
LQEMFGFQKD NVANQREHLI LLLANVHIRQ FPKPDQQPKL DDQALTEVMK KLFKNYKKWC 

       310        320        330        340        350        360 
KYLGRKSSLW LPTIQQEMQQ RKLLYMALYL LIWGEAANLR FMPECLCYIY HHMAFELYGM 

       370        380        390        400        410        420 
LAGNVSPMTG ENVKPAYGGE EDAFLRKVVT PIYEVIQMEA QRSKKGKSKH SQWRNYDDLN 

       430        440        450        460        470        480 
EYFWSVDCFR LGWPMRADAD FFCLPVAVPN TEKDGDNSKP IVARDRWVGK VNFVEIRSFW 

       490        500        510        520        530        540 
HVFRSFDRMW SFYILCLQAM IIMAWDGGQP SSVFGADVFK KVLSVFITAA IMKLGQAVLD 

       550        560        570        580        590        600 
VILNFKAHQS MTLHVKLRYI LKVFSAAAWV IILPVTYAYS WKDPPAFART IKSWFGSAMH 

       610        620        630        640        650        660 
SPSLFIIAVV SYLSPNMLAG VMFLFPLLRR FLERSNYRIV MLMMWWSQPR LYVGRGMHES 

       670        680        690        700        710        720 
AFSLFKYTMF WVLLIATKLA FSYYIEIRPL VAPTQAIMKA RVTNFQWHEF FPRAKNNIGV 

       730        740        750        760        770        780 
VIALWAPIIL VYFMDSQIWY AIFSTLFGGI YGAFRRLGEI RTLGMLRSRF ESLPGAFNDR 

       790        800        810        820        830        840 
LIPDGKNQQK KKGIRATLSH NFTEDKVPVN KEKEAARFAQ LWNTIISSFR EEDLISDREM 

       850        860        870        880        890        900 
DLLLVPYWAD RDLDLIQWPP FLLASKIPIA LDMAKDSNGK DRELKKRIES DTYMKCAVRE 

       910        920        930        940        950        960 
CYASFKNIIK FVVQGNREKE VIEIIFAEVD KHIDTGDLIQ EYKMSALPSL YDHFVKLIKY 

       970        980        990       1000       1010       1020 
LLDNKEEDRD HVVILFQDML EVVTRDIMME DYNISSLVDS SHGGTWHGGM IPLEQQYQLF 

      1030       1040       1050       1060       1070       1080 
ASSGAIRFPI EPVTEAWKEK IKRIYLLLTT KESAMDVPSN LEARRRISFF SNSLFMDMPM 

      1090       1100       1110       1120       1130       1140 
APKVRNMLSF SVLTPYYTEE VLFSLRDLET PNEDGVSILF YLQKIFPDEW NNFLERVKCL 

      1150       1160       1170       1180       1190       1200 
SEEELKESDE LEEELRLWAS YRGQTLTRTV RGMMYYRKAL ELQAFLDMAM HEDLMEGYKA 

      1210       1220       1230       1240       1250       1260 
VELNSENNSR GERSLWAQCQ AVADMKFTYV VSCQQYGIHK RSGDPRAQDI LRLMTRYPSL 

      1270       1280       1290       1300       1310       1320 
RVAYIDEVEE PVKDKSKKGN QKVYYSVLVK VPKSTDHSTL AQNLDQVIYR IRLPGPAILG 

      1330       1340       1350       1360       1370       1380 
EGKPENQNHA IIFSRGEGLQ TIDMNQDNYM EEALKMRNLL QEFLTKHDGV RHPSILGLRE 

      1390       1400       1410       1420       1430       1440 
HIFTGSVSSL AWFMSNQETS FVTIGQRLLA NPLRVRFHYG HPDVFDRLFH LTRGGVSKAS 

      1450       1460       1470       1480       1490       1500 
KVINLSEDIF AGFNSTLREG NVTHHEYIQV GKGRDVGLNQ ISMFEAKIAN GNGEQTLSRD 

      1510       1520       1530       1540       1550       1560 
IYRLGHRFDF FRMMSCYFTT VGFYFSTLIT VLTVYIFLYG RLYLVLSGLE QGLSTQKGIR 

      1570       1580       1590       1600       1610       1620 
DNTPLQIALA SQSFVQIGFL MALPMLMEIG LERGFRTALS EFVLMQLQLA PVFFTFSLGT 

      1630       1640       1650       1660       1670       1680 
KTHYYGRTLL HGGAKYRSTG RGFVVFHAKF ADNYRLYSRS HFVKGLEMML LLVVYQIFGS 

      1690       1700       1710       1720       1730       1740 
AYRGVLAYLL ITISMWFMVG TWLFAPFLFN PSGFEWQKIV DDWTDWNKWI NNIGGIGVPA 

      1750       1760       1770       1780       1790       1800 
EKSWESWWEE EQEHLRYSGK RGIVVEILLA LRFFIYQYGL VYHLTITEKT KNFLVYGVSW 

      1810       1820       1830       1840       1850       1860 
LVIFLILFVM KTVSVGRRRF SASFQLMFRL IKGLIFMTFI AIIVILITLA HMTIQDIIVC 

      1870       1880       1890       1900       1910       1920 
ILAFMPTGWG MLLIAQACKP VVHRAGFWGS VRTLARGYEI VMGLLLFTPV AFLAWFPFVS 

      1930       1940       1950 
EFQTRMLFNQ AFSRGLQISR ILGGHRKDRS SRNKE

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed: 11130714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[4]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL353013 Genomic DNA. Translation: CAB88264.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED91839.1.
IPIIPI00533855.
PIRT49914.
RefSeqNP_196804.6. NM_121303.7.
UniGeneAt.32120.

3D structure databases

ProteinModelPortalQ9LXT9.
ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ9LXT9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID831140.
GenomeReviewsGene locus AT5G13000 in contig BA000015_GR.

Organism-specific databases

TAIRAt5g13000.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000028111.
HOGENOMHBG316390.
InParanoidQ9LXT9.

Gene expression databases

GenevestigatorQ9LXT9.

Family and domain databases

InterProIPR006745. DUF605.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1_N.
[Graphical view]
Gene3DG3DSA:1.25.40.270. G3DSA:1.25.40.270. 1 hit.
PfamPF04652. DUF605. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALS3_ARATH
AccessionPrimary (citable) accession number: Q9LXT9
Secondary accession number(s): F4K2E8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 16, 2011
Last modified: January 25, 2012
This is version 49 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents