Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9LXS7 (CISY1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase 1, peroxisomal

EC=2.3.3.1
Gene names
Name:CSY1
Ordered Locus Names:At3g58740
ORF Names:T20N10.90
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subcellular location

Peroxisome Potential.

Tissue specificity

Expressed only in siliques. Not expressed in flower, stem, cauline leaf, young leaf, mature leaf and senescent leaf. Ref.4

Developmental stage

Weakly or not expressed during seedling growth. Ref.4

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentPeroxisome
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentperoxisome

Inferred from sequence or structural similarity Ref.4. Source: TAIR

   Molecular_functioncitrate (Si)-synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Peroxisome Potential
Chain? – 480Citrate synthase 1, peroxisomalPRO_0000005481

Sites

Active site3211 By similarity
Active site3601 By similarity
Active site4161 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LXS7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 51E680C04B438C92

FASTA48052,895
        10         20         30         40         50         60 
MEISERARAR LAVLNAHLTV SEPNQVLPAI EPWCTSAHIT AAPHGSLKGN LKIVDERTGN 

        70         80         90        100        110        120 
EYQVPVSEHG TVKTVDLKKI TTGKDDKGLN LYDPGYLNTA PVRSSISYID GDEGILRYRG 

       130        140        150        160        170        180 
YPVEELAEKS TYTEVTYLLI YGNLPSQRQL ADWEFAISQN SAVPQGVLDM IQSMPNDVHP 

       190        200        210        220        230        240 
VGALVTAMSA LSIFYPDANP SLMGLGVYKS KQVRDKQIVR VLGQAPTIAA AAYLRKAGKP 

       250        260        270        280        290        300 
PVQPLSNLSY SENFLYMVES MGDRSYKPNP RLARVLDILF ILQAEHEMNC STAAARHLSS 

       310        320        330        340        350        360 
SGGDVYTAVS GGVGAIYGPL HGGAVEATIN MLSEIGTVEN IPEFIESVKN KKRRLSGFGH 

       370        380        390        400        410        420 
RIYKNYDPRG KVVKKLADEV FSILGRDPLV EVGDALEKAA LSDEYFVKRK LYPNVDFYSG 

       430        440        450        460        470        480 
LINRAMGIPS SFTAVSRIAG YLSHWRESLD DPDTKIMRPQ QVYTGAGIRH YETVRERTKL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination."
Pracharoenwattana I., Cornah J.E., Smith S.M.
Plant Cell 17:2037-2048(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL353032 Genomic DNA. Translation: CAB88291.1.
CP002686 Genomic DNA. Translation: AEE79825.1.
AY099611 mRNA. Translation: AAM20462.1.
BT002169 mRNA. Translation: AAN72180.1.
PIRT49157.
RefSeqNP_191433.1. NM_115736.2.

3D structure databases

ProteinModelPortalQ9LXS7.
SMRQ9LXS7. Positions 49-477.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid10358. 1 interaction.
IntActQ9LXS7. 1 interaction.
STRING3702.AT3G58740.1-P.

Proteomic databases

PaxDbQ9LXS7.
PRIDEQ9LXS7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G58740.1; AT3G58740.1; AT3G58740.
GeneID825043.
KEGGath:AT3G58740.

Organism-specific databases

TAIRAT3G58740.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021224.
InParanoidQ9LXS7.
KOK01647.
OMAVKETKIC.
PhylomeDBQ9LXS7.
ProtClustDBPLN02456.

Enzyme and pathway databases

BioCycARA:AT3G58740-MONOMER.
UniPathwayUPA00223; UER00717.

Gene expression databases

GenevestigatorQ9LXS7.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY1_ARATH
AccessionPrimary (citable) accession number: Q9LXS7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2000
Last modified: March 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names