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Q9LXS6 (CISY2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase 2, peroxisomal

EC=2.3.3.1
Gene names
Name:CSY2
Ordered Locus Names:At3g58750
ORF Names:T20N10.100
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Peroxisomal citrate synthase required for the fatty acid respiration in seedlings, citrate being exported from peroxisomes into mitochondria during respiration of triacylglycerol (TAG). Indeed, complete respiration requires the transfer of carbon in the form of citrate from the peroxisome to the mitochondria. Ref.5

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subcellular location

Peroxisome Ref.5.

Tissue specificity

Widely expressed. Expressed throughout the shoot. Expressed in flower, silique, stem, cauline leaf, young leaf, mature leaf and senescent leaf. Ref.5

Developmental stage

Expressed throughout seedling growth. Ref.5

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Sequence caution

The sequence BAD93857.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Peroxisome Potential
Chain? – 514Citrate synthase 2, peroxisomalPRO_0000005482

Sites

Active site3241 By similarity
Active site3631 By similarity
Active site4191 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LXS6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 1226E3B4AFF662B7

FASTA51456,603
        10         20         30         40         50         60 
MEISQRVKAR LAVLTAHLAV SDTVGLEQVL PAIAPWCTSA HITAAPHGSL KGNLTIVDER 

        70         80         90        100        110        120 
TGKKYQVPVS EHGTVKAVDL KKITTGKDDK GLKLYDPGYL NTAPVRSSIC YIDGDEGILR 

       130        140        150        160        170        180 
YRGYPIEELA ESSTFIEVAY LLMYGNLPSQ SQLADWEFTV SQHSAVPQGV LDIIQSMPHD 

       190        200        210        220        230        240 
AHPMGVLVSA MSALSIFHPD ANPALSGQDI YKSKQVRDKQ IVRILGKAPT IAAAAYLRTA 

       250        260        270        280        290        300 
GRPPVLPSAN LSYSENFLYM LDSMGNRSYK PNPRLARVLD ILFILHAEHE MNCSTAAARH 

       310        320        330        340        350        360 
LASSGVDVYT ACAGAVGALY GPLHGGANEA VLKMLAEIGT AENIPDFIEG VKNRKRKMSG 

       370        380        390        400        410        420 
FGHRVYKNYD PRAKVIKKLA DEVFSIVGRD PLIEVAVALE KAALSDEYFV KRKLYPNVDF 

       430        440        450        460        470        480 
YSGLIYRAMG FPPEFFTVLF AVPRMAGYLS HWRESLDDPD TRIMRPQQAY TGVWMRHYEP 

       490        500        510 
VRERTLSSDS DKDKFGQVSI SNASRRRLAG SSAL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 408-514.
Strain: cv. Columbia.
[5]"Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination."
Pracharoenwattana I., Cornah J.E., Smith S.M.
Plant Cell 17:2037-2048(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL353032 Genomic DNA. Translation: CAB88292.1.
CP002686 Genomic DNA. Translation: AEE79826.1.
BT015884 mRNA. Translation: AAU95420.1.
BT020195 mRNA. Translation: AAV59261.1.
AK220724 mRNA. Translation: BAD93857.1. Different initiation.
PIRT49158.
RefSeqNP_191434.1. NM_115737.2.

3D structure databases

ProteinModelPortalQ9LXS6.
SMRQ9LXS6. Positions 52-484.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid10359. 5 interactions.
IntActQ9LXS6. 4 interactions.

Proteomic databases

PaxDbQ9LXS6.
PRIDEQ9LXS6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G58750.1; AT3G58750.1; AT3G58750.
GeneID825044.
KEGGath:AT3G58750.

Organism-specific databases

TAIRAT3G58750.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021224.
InParanoidQ9LXS6.
KOK01647.
OMAVYHSLGI.
PhylomeDBQ9LXS6.
ProtClustDBPLN02456.

Enzyme and pathway databases

BioCycARA:AT3G58750-MONOMER.
UniPathwayUPA00223; UER00717.

Gene expression databases

GenevestigatorQ9LXS6.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY2_ARATH
AccessionPrimary (citable) accession number: Q9LXS6
Secondary accession number(s): Q570I4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2000
Last modified: March 19, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names