Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9LXS6

- CISY2_ARATH

UniProt

Q9LXS6 - CISY2_ARATH

Protein

Citrate synthase 2, peroxisomal

Gene

CSY2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Peroxisomal citrate synthase required for the fatty acid respiration in seedlings, citrate being exported from peroxisomes into mitochondria during respiration of triacylglycerol (TAG). Indeed, complete respiration requires the transfer of carbon in the form of citrate from the peroxisome to the mitochondria.1 Publication

    Catalytic activityi

    Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei324 – 3241PROSITE-ProRule annotation
    Active sitei363 – 3631PROSITE-ProRule annotation
    Active sitei419 – 4191PROSITE-ProRule annotation

    GO - Molecular functioni

    1. citrate (Si)-synthase activity Source: TAIR

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. fatty acid beta-oxidation Source: TAIR
    3. tricarboxylic acid cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciARA:AT3G58750-MONOMER.
    UniPathwayiUPA00223; UER00717.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Citrate synthase 2, peroxisomal (EC:2.3.3.16)
    Gene namesi
    Name:CSY2
    Ordered Locus Names:At3g58750
    ORF Names:T20N10.100
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G58750.

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisome Source: TAIR
    2. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 514Citrate synthase 2, peroxisomalPRO_0000005482
    Transit peptidei1 – ?PeroxisomeSequence Analysis

    Proteomic databases

    PaxDbiQ9LXS6.
    PRIDEiQ9LXS6.

    Expressioni

    Tissue specificityi

    Widely expressed. Expressed throughout the shoot. Expressed in flower, silique, stem, cauline leaf, young leaf, mature leaf and senescent leaf.1 Publication

    Developmental stagei

    Expressed throughout seedling growth.1 Publication

    Gene expression databases

    GenevestigatoriQ9LXS6.

    Interactioni

    Protein-protein interaction databases

    BioGridi10359. 5 interactions.
    IntActiQ9LXS6. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXS6.
    SMRiQ9LXS6. Positions 62-484.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the citrate synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0372.
    HOGENOMiHOG000021224.
    InParanoidiQ9LXS6.
    KOiK01647.
    OMAiKFPTIVA.
    PhylomeDBiQ9LXS6.

    Family and domain databases

    Gene3Di1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view]
    PANTHERiPTHR11739. PTHR11739. 1 hit.
    PfamiPF00285. Citrate_synt. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001369. Citrate_synth. 1 hit.
    PRINTSiPR00143. CITRTSNTHASE.
    SUPFAMiSSF48256. SSF48256. 1 hit.
    PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LXS6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEISQRVKAR LAVLTAHLAV SDTVGLEQVL PAIAPWCTSA HITAAPHGSL    50
    KGNLTIVDER TGKKYQVPVS EHGTVKAVDL KKITTGKDDK GLKLYDPGYL 100
    NTAPVRSSIC YIDGDEGILR YRGYPIEELA ESSTFIEVAY LLMYGNLPSQ 150
    SQLADWEFTV SQHSAVPQGV LDIIQSMPHD AHPMGVLVSA MSALSIFHPD 200
    ANPALSGQDI YKSKQVRDKQ IVRILGKAPT IAAAAYLRTA GRPPVLPSAN 250
    LSYSENFLYM LDSMGNRSYK PNPRLARVLD ILFILHAEHE MNCSTAAARH 300
    LASSGVDVYT ACAGAVGALY GPLHGGANEA VLKMLAEIGT AENIPDFIEG 350
    VKNRKRKMSG FGHRVYKNYD PRAKVIKKLA DEVFSIVGRD PLIEVAVALE 400
    KAALSDEYFV KRKLYPNVDF YSGLIYRAMG FPPEFFTVLF AVPRMAGYLS 450
    HWRESLDDPD TRIMRPQQAY TGVWMRHYEP VRERTLSSDS DKDKFGQVSI 500
    SNASRRRLAG SSAL 514
    Length:514
    Mass (Da):56,603
    Last modified:October 1, 2000 - v1
    Checksum:i1226E3B4AFF662B7
    GO

    Sequence cautioni

    The sequence BAD93857.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353032 Genomic DNA. Translation: CAB88292.1.
    CP002686 Genomic DNA. Translation: AEE79826.1.
    BT015884 mRNA. Translation: AAU95420.1.
    BT020195 mRNA. Translation: AAV59261.1.
    AK220724 mRNA. Translation: BAD93857.1. Different initiation.
    PIRiT49158.
    RefSeqiNP_191434.1. NM_115737.2.

    Genome annotation databases

    EnsemblPlantsiAT3G58750.1; AT3G58750.1; AT3G58750.
    GeneIDi825044.
    KEGGiath:AT3G58750.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353032 Genomic DNA. Translation: CAB88292.1 .
    CP002686 Genomic DNA. Translation: AEE79826.1 .
    BT015884 mRNA. Translation: AAU95420.1 .
    BT020195 mRNA. Translation: AAV59261.1 .
    AK220724 mRNA. Translation: BAD93857.1 . Different initiation.
    PIRi T49158.
    RefSeqi NP_191434.1. NM_115737.2.

    3D structure databases

    ProteinModelPortali Q9LXS6.
    SMRi Q9LXS6. Positions 62-484.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 10359. 5 interactions.
    IntActi Q9LXS6. 4 interactions.

    Proteomic databases

    PaxDbi Q9LXS6.
    PRIDEi Q9LXS6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G58750.1 ; AT3G58750.1 ; AT3G58750 .
    GeneIDi 825044.
    KEGGi ath:AT3G58750.

    Organism-specific databases

    TAIRi AT3G58750.

    Phylogenomic databases

    eggNOGi COG0372.
    HOGENOMi HOG000021224.
    InParanoidi Q9LXS6.
    KOi K01647.
    OMAi KFPTIVA.
    PhylomeDBi Q9LXS6.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER00717 .
    BioCyci ARA:AT3G58750-MONOMER.

    Gene expression databases

    Genevestigatori Q9LXS6.

    Family and domain databases

    Gene3Di 1.10.230.10. 1 hit.
    1.10.580.10. 1 hit.
    InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
    IPR016143. Citrate_synth-like_sm_a-sub.
    IPR002020. Citrate_synthase-like.
    IPR016141. Citrate_synthase-like_core.
    IPR019810. Citrate_synthase_AS.
    IPR024176. Citrate_synthase_bac-typ.
    [Graphical view ]
    PANTHERi PTHR11739. PTHR11739. 1 hit.
    Pfami PF00285. Citrate_synt. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001369. Citrate_synth. 1 hit.
    PRINTSi PR00143. CITRTSNTHASE.
    SUPFAMi SSF48256. SSF48256. 1 hit.
    PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 408-514.
      Strain: cv. Columbia.
    5. "Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination."
      Pracharoenwattana I., Cornah J.E., Smith S.M.
      Plant Cell 17:2037-2048(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiCISY2_ARATH
    AccessioniPrimary (citable) accession number: Q9LXS6
    Secondary accession number(s): Q570I4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Citrate synthase is found in nearly all cells capable of oxidative metabolism.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3