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Q9LXL5

- SUS4_ARATH

UniProt

Q9LXL5 - SUS4_ARATH

Protein

Sucrose synthase 4

Gene

SUS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.By similarity

    Catalytic activityi

    NDP-glucose + D-fructose = NDP + sucrose.

    Kineticsi

    1. KM=4.97 mM for D-fructose (synthetic reaction) at pH 9.41 Publication
    2. KM=0.07 mM for UDP-glucose (synthetic reaction) at pH 9.41 Publication
    3. KM=67.54 mM for sucrose (degradative reaction) at pH 61 Publication
    4. KM=0.07 mM for UDP (degradative reaction) at pH 61 Publication

    Vmax=12.01 µmol/min/mg enzyme for synthetic reaction at pH 9.41 Publication

    Vmax=4.99 µmol/min/mg enzyme for degradative reaction at pH 61 Publication

    pH dependencei

    Optimum pH is 6.0-7.0 for degradative reaction and 7.0 (PubMed:22184213) or 9.0-9.5 (PubMed:17257168) for synthetic reaction.1 Publication

    GO - Molecular functioni

    1. sucrose synthase activity Source: TAIR

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. response to hypoxia Source: UniProtKB
    3. sucrose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciMetaCyc:AT3G43190-MONOMER.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sucrose synthase 4 (EC:2.4.1.13)
    Short name:
    AtSUS4
    Alternative name(s):
    Sucrose-UDP glucosyltransferase 4
    Gene namesi
    Name:SUS4
    Ordered Locus Names:At3g43190
    ORF Names:F7K15_40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G43190.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. membrane Source: TAIR
    3. plasma membrane Source: TAIR
    4. vacuole Source: TAIR

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 808808Sucrose synthase 4PRO_0000418803Add
    BLAST

    Proteomic databases

    PRIDEiQ9LXL5.

    Expressioni

    Tissue specificityi

    Detected in the whole plant with highest expression in young rosette leaves and roots.3 Publications

    Inductioni

    By anaerobic stress. By hypoxia in the roots (at protein level). Up-regulated by NUC/IDD8.3 Publications

    Gene expression databases

    GenevestigatoriQ9LXL5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G43190.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXL5.
    SMRiQ9LXL5. Positions 27-807.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni277 – 754478GT-B glycosyltransferaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0438.
    HOGENOMiHOG000240125.
    InParanoidiQ9LXL5.
    KOiK00695.
    OMAiCAKRAQE.
    PhylomeDBiQ9LXL5.

    Family and domain databases

    InterProiIPR001296. Glyco_trans_1.
    IPR000368. Sucrose_synth.
    IPR012820. Sucrose_synthase_pln/cyn.
    [Graphical view]
    PfamiPF00534. Glycos_transf_1. 1 hit.
    PF00862. Sucrose_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9LXL5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANAERVITR VHSQRERLDA TLVAQKNEVF ALLSRVEAKG KGILQHHQII    50
    AEFEAMPLET QKKLKGGAFF EFLRSAQEAI VLPPFVALAV RPRPGVWEYV 100
    RVNLHDLVVE ELQASEYLQF KEELVDGIKN GNFTLELDFE PFNAAFPRPT 150
    LNKYIGDGVE FLNRHLSAKL FHDKESLHPL LKFLRLHSHE GKTLMLNNRI 200
    QNLNTLQHNL RKAEEYLMEL KPETLYSEFE HKFQEIGLER GWGDTAERVL 250
    NMIRLLLDLL EAPDPCTLEN FLGRIPMVFN VVILSPHGYF AQDNVLGYPD 300
    TGGQVVYILD QVRALETEML QRIKQQGLNI TPRILIITRL LPDAAGTTCG 350
    QRLEKVYGSQ YCDILRVPFR TEKGIVRKWI SRFEVWPYLE TFTEDVAAEI 400
    SKELQGKPDL IIGNYSDGNL VASLLAHKLG VTQCTIAHAL EKTKYPDSDI 450
    YWKKLDEKYH FSCQFTADLI AMNHTDFIIT STFQEIAGSK DTVGQYESHR 500
    SFTLPGLYRV VHGIDVFDPK FNIVSPGADM SIYFAYTEEK RRLTAFHLEI 550
    EELLYSDVEN EEHLCVLKDK KKPIIFTMAR LDRVKNLSGL VEWYGKNTRL 600
    RELVNLVVVG GDRRKESQDN EEKAEMKKMY ELIEEYKLNG QFRWISSQMN 650
    RVRNGELYRY ICDTKGAFVQ PALYEAFGLT VVEAMTCGLP TFATCNGGPA 700
    EIIVHGKSGF HIDPYHGDKA AESLADFFTK CKHDPSHWDQ ISLGGLERIQ 750
    EKYTWQIYSQ RLLTLTGVYG FWKHVSNLDR LESRRYLEMF YALKYRPLAQ 800
    AVPLAHEE 808
    Length:808
    Mass (Da):93,003
    Last modified:October 1, 2000 - v1
    Checksum:iC9FDF33B6325CFBB
    GO

    Sequence cautioni

    The sequence AAK59464.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti395 – 3951D → V in BAE99649. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353871 Genomic DNA. Translation: CAB89040.1.
    CP002686 Genomic DNA. Translation: AEE77773.1.
    AK227662 mRNA. Translation: BAE99649.1.
    AY034958 mRNA. Translation: AAK59464.1. Different initiation.
    PIRiT49233.
    RefSeqiNP_566865.2. NM_114187.4.
    UniGeneiAt.1720.

    Genome annotation databases

    EnsemblPlantsiAT3G43190.1; AT3G43190.1; AT3G43190.
    GeneIDi823393.
    KEGGiath:AT3G43190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353871 Genomic DNA. Translation: CAB89040.1 .
    CP002686 Genomic DNA. Translation: AEE77773.1 .
    AK227662 mRNA. Translation: BAE99649.1 .
    AY034958 mRNA. Translation: AAK59464.1 . Different initiation.
    PIRi T49233.
    RefSeqi NP_566865.2. NM_114187.4.
    UniGenei At.1720.

    3D structure databases

    ProteinModelPortali Q9LXL5.
    SMRi Q9LXL5. Positions 27-807.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G43190.1-P.

    Protein family/group databases

    CAZyi GT4. Glycosyltransferase Family 4.

    Proteomic databases

    PRIDEi Q9LXL5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G43190.1 ; AT3G43190.1 ; AT3G43190 .
    GeneIDi 823393.
    KEGGi ath:AT3G43190.

    Organism-specific databases

    TAIRi AT3G43190.

    Phylogenomic databases

    eggNOGi COG0438.
    HOGENOMi HOG000240125.
    InParanoidi Q9LXL5.
    KOi K00695.
    OMAi CAKRAQE.
    PhylomeDBi Q9LXL5.

    Enzyme and pathway databases

    BioCyci MetaCyc:AT3G43190-MONOMER.

    Gene expression databases

    Genevestigatori Q9LXL5.

    Family and domain databases

    InterProi IPR001296. Glyco_trans_1.
    IPR000368. Sucrose_synth.
    IPR012820. Sucrose_synthase_pln/cyn.
    [Graphical view ]
    Pfami PF00534. Glycos_transf_1. 1 hit.
    PF00862. Sucrose_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02470. sucr_synth. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-395.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-808.
      Strain: cv. Columbia.
    5. "Structure and expression profile of the sucrose synthase multigene family in Arabidopsis."
      Baud S., Vaultier M.N., Rochat C.
      J. Exp. Bot. 55:397-409(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, TISSUE SPECIFICITY, INDUCTION.
    6. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
    7. "Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development."
      Fallahi H., Scofield G.N., Badger M.R., Chow W.S., Furbank R.T., Ruan Y.L.
      J. Exp. Bot. 59:3283-3295(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Modulation of sugar metabolism by an INDETERMINATE DOMAIN transcription factor contributes to photoperiodic flowering in Arabidopsis."
      Seo P.J., Ryu J., Kang S.K., Park C.M.
      Plant J. 65:418-429(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY NUC.

    Entry informationi

    Entry nameiSUS4_ARATH
    AccessioniPrimary (citable) accession number: Q9LXL5
    Secondary accession number(s): Q0WT99, Q94CC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3