Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sucrose synthase 4

Gene

SUS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.By similarity

Catalytic activityi

NDP-glucose + D-fructose = NDP + sucrose.

Kineticsi

  1. KM=4.97 mM for D-fructose (synthetic reaction) at pH 9.41 Publication
  2. KM=0.07 mM for UDP-glucose (synthetic reaction) at pH 9.41 Publication
  3. KM=67.54 mM for sucrose (degradative reaction) at pH 61 Publication
  4. KM=0.07 mM for UDP (degradative reaction) at pH 61 Publication

Vmax=12.01 µmol/min/mg enzyme for synthetic reaction at pH 9.41 Publication

Vmax=4.99 µmol/min/mg enzyme for degradative reaction at pH 61 Publication

pH dependencei

Optimum pH is 6.0-7.0 for degradative reaction and 7.0 (PubMed:22184213) or 9.0-9.5 (PubMed:17257168) for synthetic reaction.1 Publication

GO - Molecular functioni

  1. sucrose synthase activity Source: TAIR

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. response to hypoxia Source: UniProtKB
  3. sucrose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciMetaCyc:AT3G43190-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose synthase 4 (EC:2.4.1.13)
Short name:
AtSUS4
Alternative name(s):
Sucrose-UDP glucosyltransferase 4
Gene namesi
Name:SUS4
Ordered Locus Names:At3g43190
ORF Names:F7K15_40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G43190.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. membrane Source: TAIR
  3. plasma membrane Source: TAIR
  4. vacuole Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 808808Sucrose synthase 4PRO_0000418803Add
BLAST

Proteomic databases

PRIDEiQ9LXL5.

Expressioni

Tissue specificityi

Detected in the whole plant with highest expression in young rosette leaves and roots.3 Publications

Inductioni

By anaerobic stress. By hypoxia in the roots (at protein level). Up-regulated by NUC/IDD8.3 Publications

Gene expression databases

GenevestigatoriQ9LXL5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G43190.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LXL5.
SMRiQ9LXL5. Positions 27-807.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni277 – 754478GT-B glycosyltransferaseBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000240125.
InParanoidiQ9LXL5.
KOiK00695.
OMAiRVWETIS.
PhylomeDBiQ9LXL5.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LXL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANAERVITR VHSQRERLDA TLVAQKNEVF ALLSRVEAKG KGILQHHQII
60 70 80 90 100
AEFEAMPLET QKKLKGGAFF EFLRSAQEAI VLPPFVALAV RPRPGVWEYV
110 120 130 140 150
RVNLHDLVVE ELQASEYLQF KEELVDGIKN GNFTLELDFE PFNAAFPRPT
160 170 180 190 200
LNKYIGDGVE FLNRHLSAKL FHDKESLHPL LKFLRLHSHE GKTLMLNNRI
210 220 230 240 250
QNLNTLQHNL RKAEEYLMEL KPETLYSEFE HKFQEIGLER GWGDTAERVL
260 270 280 290 300
NMIRLLLDLL EAPDPCTLEN FLGRIPMVFN VVILSPHGYF AQDNVLGYPD
310 320 330 340 350
TGGQVVYILD QVRALETEML QRIKQQGLNI TPRILIITRL LPDAAGTTCG
360 370 380 390 400
QRLEKVYGSQ YCDILRVPFR TEKGIVRKWI SRFEVWPYLE TFTEDVAAEI
410 420 430 440 450
SKELQGKPDL IIGNYSDGNL VASLLAHKLG VTQCTIAHAL EKTKYPDSDI
460 470 480 490 500
YWKKLDEKYH FSCQFTADLI AMNHTDFIIT STFQEIAGSK DTVGQYESHR
510 520 530 540 550
SFTLPGLYRV VHGIDVFDPK FNIVSPGADM SIYFAYTEEK RRLTAFHLEI
560 570 580 590 600
EELLYSDVEN EEHLCVLKDK KKPIIFTMAR LDRVKNLSGL VEWYGKNTRL
610 620 630 640 650
RELVNLVVVG GDRRKESQDN EEKAEMKKMY ELIEEYKLNG QFRWISSQMN
660 670 680 690 700
RVRNGELYRY ICDTKGAFVQ PALYEAFGLT VVEAMTCGLP TFATCNGGPA
710 720 730 740 750
EIIVHGKSGF HIDPYHGDKA AESLADFFTK CKHDPSHWDQ ISLGGLERIQ
760 770 780 790 800
EKYTWQIYSQ RLLTLTGVYG FWKHVSNLDR LESRRYLEMF YALKYRPLAQ

AVPLAHEE
Length:808
Mass (Da):93,003
Last modified:October 1, 2000 - v1
Checksum:iC9FDF33B6325CFBB
GO

Sequence cautioni

The sequence AAK59464.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti395 – 3951D → V in BAE99649. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353871 Genomic DNA. Translation: CAB89040.1.
CP002686 Genomic DNA. Translation: AEE77773.1.
AK227662 mRNA. Translation: BAE99649.1.
AY034958 mRNA. Translation: AAK59464.1. Different initiation.
PIRiT49233.
RefSeqiNP_566865.2. NM_114187.4.
UniGeneiAt.1720.

Genome annotation databases

EnsemblPlantsiAT3G43190.1; AT3G43190.1; AT3G43190.
GeneIDi823393.
KEGGiath:AT3G43190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353871 Genomic DNA. Translation: CAB89040.1.
CP002686 Genomic DNA. Translation: AEE77773.1.
AK227662 mRNA. Translation: BAE99649.1.
AY034958 mRNA. Translation: AAK59464.1. Different initiation.
PIRiT49233.
RefSeqiNP_566865.2. NM_114187.4.
UniGeneiAt.1720.

3D structure databases

ProteinModelPortaliQ9LXL5.
SMRiQ9LXL5. Positions 27-807.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G43190.1-P.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEiQ9LXL5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G43190.1; AT3G43190.1; AT3G43190.
GeneIDi823393.
KEGGiath:AT3G43190.

Organism-specific databases

TAIRiAT3G43190.

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000240125.
InParanoidiQ9LXL5.
KOiK00695.
OMAiRVWETIS.
PhylomeDBiQ9LXL5.

Enzyme and pathway databases

BioCyciMetaCyc:AT3G43190-MONOMER.

Gene expression databases

GenevestigatoriQ9LXL5.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
IPR000368. Sucrose_synth.
IPR012820. Sucrose_synthase_pln/cyn.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
PF00862. Sucrose_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02470. sucr_synth. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-395.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-808.
    Strain: cv. Columbia.
  5. "Structure and expression profile of the sucrose synthase multigene family in Arabidopsis."
    Baud S., Vaultier M.N., Rochat C.
    J. Exp. Bot. 55:397-409(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY, INDUCTION.
  6. Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  7. "Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development."
    Fallahi H., Scofield G.N., Badger M.R., Chow W.S., Furbank R.T., Ruan Y.L.
    J. Exp. Bot. 59:3283-3295(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Modulation of sugar metabolism by an INDETERMINATE DOMAIN transcription factor contributes to photoperiodic flowering in Arabidopsis."
    Seo P.J., Ryu J., Kang S.K., Park C.M.
    Plant J. 65:418-429(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NUC.

Entry informationi

Entry nameiSUS4_ARATH
AccessioniPrimary (citable) accession number: Q9LXL5
Secondary accession number(s): Q0WT99, Q94CC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.