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Protein

Sucrose synthase 4

Gene

SUS4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sucrose-cleaving enzyme that provides UDP-glucose and fructose for various metabolic pathways.By similarity

Catalytic activityi

NDP-glucose + D-fructose = NDP + sucrose.

Kineticsi

  1. KM=4.97 mM for D-fructose (synthetic reaction) at pH 9.41 Publication
  2. KM=0.07 mM for UDP-glucose (synthetic reaction) at pH 9.41 Publication
  3. KM=67.54 mM for sucrose (degradative reaction) at pH 61 Publication
  4. KM=0.07 mM for UDP (degradative reaction) at pH 61 Publication
  1. Vmax=12.01 µmol/min/mg enzyme for synthetic reaction at pH 9.41 Publication
  2. Vmax=4.99 µmol/min/mg enzyme for degradative reaction at pH 61 Publication

pH dependencei

Optimum pH is 6.0-7.0 for degradative reaction (PubMed:22184213, PubMed:17257168). Optimum pH is 7.0 for synthetic reaction (PubMed:22184213). Optimum pH is 9.0-9.5 for synthetic reaction (PubMed:17257168).1 Publication

GO - Molecular functioni

  • sucrose synthase activity Source: TAIR

GO - Biological processi

  • response to hypoxia Source: UniProtKB
  • sucrose metabolic process Source: InterPro

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processStress response

Enzyme and pathway databases

BioCyciMetaCyc:AT3G43190-MONOMER
BRENDAi2.4.1.13 399

Protein family/group databases

CAZyiGT4 Glycosyltransferase Family 4

Names & Taxonomyi

Protein namesi
Recommended name:
Sucrose synthase 4 (EC:2.4.1.13)
Short name:
AtSUS4
Alternative name(s):
Sucrose-UDP glucosyltransferase 4
Gene namesi
Name:SUS4
Ordered Locus Names:At3g43190
ORF Names:F7K15_40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G43190
TAIRilocus:2084756 AT3G43190

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004188031 – 808Sucrose synthase 4Add BLAST808

Proteomic databases

PaxDbiQ9LXL5
PRIDEiQ9LXL5

PTM databases

iPTMnetiQ9LXL5
SwissPalmiQ9LXL5

Expressioni

Tissue specificityi

Detected in the whole plant with highest expression in young rosette leaves and roots.3 Publications

Inductioni

By anaerobic stress. By hypoxia in the roots (at protein level). Up-regulated by NUC/IDD8.3 Publications

Gene expression databases

ExpressionAtlasiQ9LXL5 baseline and differential
GenevisibleiQ9LXL5 AT

Interactioni

Protein-protein interaction databases

BioGridi8715, 2 interactors
STRINGi3702.AT3G43190.1

Structurei

3D structure databases

ProteinModelPortaliQ9LXL5
SMRiQ9LXL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni277 – 754GT-B glycosyltransferaseBy similarityAdd BLAST478

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0853 Eukaryota
COG0438 LUCA
HOGENOMiHOG000240125
InParanoidiQ9LXL5
KOiK00695
OMAiHEGKTLM
OrthoDBiEOG0936023J
PhylomeDBiQ9LXL5

Family and domain databases

InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR000368 Sucrose_synth
IPR012820 Sucrose_synthase_pln/cyn
PfamiView protein in Pfam
PF00534 Glycos_transf_1, 1 hit
PF00862 Sucrose_synth, 1 hit
TIGRFAMsiTIGR02470 sucr_synth, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LXL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANAERVITR VHSQRERLDA TLVAQKNEVF ALLSRVEAKG KGILQHHQII
60 70 80 90 100
AEFEAMPLET QKKLKGGAFF EFLRSAQEAI VLPPFVALAV RPRPGVWEYV
110 120 130 140 150
RVNLHDLVVE ELQASEYLQF KEELVDGIKN GNFTLELDFE PFNAAFPRPT
160 170 180 190 200
LNKYIGDGVE FLNRHLSAKL FHDKESLHPL LKFLRLHSHE GKTLMLNNRI
210 220 230 240 250
QNLNTLQHNL RKAEEYLMEL KPETLYSEFE HKFQEIGLER GWGDTAERVL
260 270 280 290 300
NMIRLLLDLL EAPDPCTLEN FLGRIPMVFN VVILSPHGYF AQDNVLGYPD
310 320 330 340 350
TGGQVVYILD QVRALETEML QRIKQQGLNI TPRILIITRL LPDAAGTTCG
360 370 380 390 400
QRLEKVYGSQ YCDILRVPFR TEKGIVRKWI SRFEVWPYLE TFTEDVAAEI
410 420 430 440 450
SKELQGKPDL IIGNYSDGNL VASLLAHKLG VTQCTIAHAL EKTKYPDSDI
460 470 480 490 500
YWKKLDEKYH FSCQFTADLI AMNHTDFIIT STFQEIAGSK DTVGQYESHR
510 520 530 540 550
SFTLPGLYRV VHGIDVFDPK FNIVSPGADM SIYFAYTEEK RRLTAFHLEI
560 570 580 590 600
EELLYSDVEN EEHLCVLKDK KKPIIFTMAR LDRVKNLSGL VEWYGKNTRL
610 620 630 640 650
RELVNLVVVG GDRRKESQDN EEKAEMKKMY ELIEEYKLNG QFRWISSQMN
660 670 680 690 700
RVRNGELYRY ICDTKGAFVQ PALYEAFGLT VVEAMTCGLP TFATCNGGPA
710 720 730 740 750
EIIVHGKSGF HIDPYHGDKA AESLADFFTK CKHDPSHWDQ ISLGGLERIQ
760 770 780 790 800
EKYTWQIYSQ RLLTLTGVYG FWKHVSNLDR LESRRYLEMF YALKYRPLAQ

AVPLAHEE
Length:808
Mass (Da):93,003
Last modified:October 1, 2000 - v1
Checksum:iC9FDF33B6325CFBB
GO

Sequence cautioni

The sequence AAK59464 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti395D → V in BAE99649 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353871 Genomic DNA Translation: CAB89040.1
CP002686 Genomic DNA Translation: AEE77773.1
CP002686 Genomic DNA Translation: ANM64625.1
AK227662 mRNA Translation: BAE99649.1
AY034958 mRNA Translation: AAK59464.1 Different initiation.
PIRiT49233
RefSeqiNP_001326640.1, NM_001339091.1
NP_566865.2, NM_114187.5
UniGeneiAt.1720

Genome annotation databases

EnsemblPlantsiAT3G43190.1; AT3G43190.1; AT3G43190
AT3G43190.2; AT3G43190.2; AT3G43190
GeneIDi823393
GrameneiAT3G43190.1; AT3G43190.1; AT3G43190
AT3G43190.2; AT3G43190.2; AT3G43190
KEGGiath:AT3G43190

Similar proteinsi

Entry informationi

Entry nameiSUS4_ARATH
AccessioniPrimary (citable) accession number: Q9LXL5
Secondary accession number(s): Q0WT99, Q94CC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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