Reviewed, UniProtKB/Swiss-Prot Q9LXI4 (PPA21_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 21
    EC=3.1.3.2

Gene names

Name:	PAP21
Synonyms:	AT5
Ordered Locus Names:	At3g52810
ORF Names:	F3C22.210

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed flowers and siliques. Ref.3
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 437	412	Purple acid phosphatase 21		PRO_0000372824
Regions
	Region	333 – 335	3	Substrate binding By similarity
Sites
	Active site	306	1	Proton donor By similarity
	Metal binding	152	1	Iron By similarity
	Metal binding	179	1	Iron By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	182	1	Iron By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	296	1	Zinc By similarity
	Metal binding	333	1	Zinc By similarity
	Metal binding	335	1	Iron By similarity
	Binding site	212	1	Substrate By similarity
Amino acid modifications
	Glycosylation	30	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LXI4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B3416E8248BC5AB1	FASTA	437	50,641
	10 20 30 40 50 60 MKKMKIFGFL ISFSLFFLSP FVCQANYDSN FTRPPPRPLF IVSHGRPKFY PQQVHISLAG 70 80 90 100 110 120 KDHMRVTYTT DDLNVASMVE YGKHPKKYDK KTAGESTSYT YFFYNSGKIH HVKIGPLKPN 130 140 150 160 170 180 TKYYYRCGGH GDEFSFKTPP SKFPIEFAVA GDLGQTDWTV RTLDQIRKRD FDVFLLPGDL 190 200 210 220 230 240 SYADTHQPLW DSFGRLLETL ASTRPWMVTE GNHEIESFPT NDHISFKSYN ARWLMPHAES 250 260 270 280 290 300 LSHSNLYYSF DVAGVHTVML GSYTPYESHS DQYHWLQADL RKVDRKKTPW LVVVMHTPWY 310 320 330 340 350 360 STNKAHYGEG EKMRSALESL LYRAQVDVVF AGHVHTYERF KPIYNKKADP CGPMYITIGD 370 380 390 400 410 420 GGNREGLALR FKKPQSPLSE FRESSFGHGR LRIIDHKRAH WSWHRNNDEM SSIADEVSFE 430 SPRTSSHCHS NRYRGEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF492667 mRNA. Translation: AAM15916.1. AL353912 Genomic DNA. Translation: CAB89242.1.
IPI	IPI00535172.
PIR	T49034.
RefSeq	NP_190849.1.
UniGene	At.35260
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
ModBase	Search...
Proteomic databases
PRIDE	Q9LXI4.
Genome annotation databases
GeneID	824447.
GenomeReviews	Gene locus AT3G52810 in contig BA000014_GR.
KEGG	ath:AT3G52810.
NMPDR	fig|3702.1.peg.16553.
Organism-specific databases
TAIR	At3g52810.
Phylogenomic databases
OMA	HIYERIA.
Gene expression databases
Genevestigator	Q9LXI4.
Family and domain databases
InterPro	IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA21_ARATH

Accession

Primary (citable) accession number: Q9LXI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)