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Q9LXI4 (PPA21_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 21
EC=3.1.3.2
Gene names
Name:PAP21
Synonyms:AT5
Ordered Locus Names:At3g52810
ORF Names:F3C22.210
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length437 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed flowers and siliques. Ref.4

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionacid phosphatase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 437412Purple acid phosphatase 21
PRO_0000372824

Regions

Region333 – 3353Substrate binding By similarity

Sites

Active site3061Proton donor By similarity
Metal binding1521Iron By similarity
Metal binding1791Iron By similarity
Metal binding1791Zinc By similarity
Metal binding1821Iron By similarity
Metal binding2121Zinc By similarity
Metal binding2961Zinc By similarity
Metal binding3331Zinc By similarity
Metal binding3351Iron By similarity
Binding site2121Substrate By similarity

Amino acid modifications

Glycosylation301N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9LXI4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: B3416E8248BC5AB1

FASTA43750,641
        10         20         30         40         50         60 
MKKMKIFGFL ISFSLFFLSP FVCQANYDSN FTRPPPRPLF IVSHGRPKFY PQQVHISLAG 

        70         80         90        100        110        120 
KDHMRVTYTT DDLNVASMVE YGKHPKKYDK KTAGESTSYT YFFYNSGKIH HVKIGPLKPN 

       130        140        150        160        170        180 
TKYYYRCGGH GDEFSFKTPP SKFPIEFAVA GDLGQTDWTV RTLDQIRKRD FDVFLLPGDL 

       190        200        210        220        230        240 
SYADTHQPLW DSFGRLLETL ASTRPWMVTE GNHEIESFPT NDHISFKSYN ARWLMPHAES 

       250        260        270        280        290        300 
LSHSNLYYSF DVAGVHTVML GSYTPYESHS DQYHWLQADL RKVDRKKTPW LVVVMHTPWY 

       310        320        330        340        350        360 
STNKAHYGEG EKMRSALESL LYRAQVDVVF AGHVHTYERF KPIYNKKADP CGPMYITIGD 

       370        380        390        400        410        420 
GGNREGLALR FKKPQSPLSE FRESSFGHGR LRIIDHKRAH WSWHRNNDEM SSIADEVSFE 

       430 
SPRTSSHCHS NRYRGEI

« Hide

References

« Hide 'large scale' references
[1]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
Strain: cv. Col-1.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF492667 mRNA. Translation: AAM15916.1.
AL353912 Genomic DNA. Translation: CAB89242.1.
CP002686 Genomic DNA. Translation: AEE78996.1.
IPIIPI00535172.
PIRT49034.
RefSeqNP_190849.1. NM_115141.3.
UniGeneAt.35260.

3D structure databases

HSSPHSSP built from PDB template 4KBP based on UniProtKB P80366.
ProteinModelPortalQ9LXI4.
SMRQ9LXI4. Positions 34-430.
ModBaseSearch...

Proteomic databases

PRIDEQ9LXI4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G52810.1; AT3G52810.1; AT3G52810.
GeneID824447.
GenomeReviewsGene locus AT3G52810 in contig BA000014_GR.
KEGGath:AT3G52810.
NMPDRfig|3702.1.peg.16553.

Organism-specific databases

TAIRAt3g52810.

Phylogenomic databases

eggNOGKOG1378.
GeneTreeEPGT00070000028556.
HOGENOMHBG316723.
InParanoidQ9LXI4.
OMACGPMYIT.
PhylomeDBQ9LXI4.
ProtClustDBCLSN2684889.

Gene expression databases

GenevestigatorQ9LXI4.

Family and domain databases

InterProIPR004843. Metallo_PEstase_dom.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
[Graphical view]
Gene3DG3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMSSF49363. Purple_Pase_N. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPA21_ARATH
AccessionPrimary (citable) accession number: Q9LXI4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents