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Protein

Plant cysteine oxidase 1

Gene

PCO1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes N-terminal cysteine residues, thus preparing the protein for N-end rule pathway-mediated proteasomal degradation. Controls the preparation of ERF-VII proteins for degradation via the 26S proteasome. Not active on Cys located inside or at the C-terminus of a peptide. Represses the anaerobic response.1 Publication

Catalytic activityi

L-cysteine + O2 = 3-sulfinoalanine.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi148 – 1481Iron; catalyticBy similarity
Metal bindingi150 – 1501Iron; catalyticBy similarity
Metal bindingi211 – 2111Iron; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

  • anaerobic respiration Source: TAIR
  • detection of hypoxia Source: TAIR
  • peptidyl-cysteine oxidation Source: TAIR
  • response to hypoxia Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-1614558. Degradation of cysteine and homocysteine.

Names & Taxonomyi

Protein namesi
Recommended name:
Plant cysteine oxidase 11 Publication (EC:1.13.11.20)
Alternative name(s):
Hypoxia-responsive unknown protein 291 Publication
Gene namesi
Name:PCO11 Publication
Synonyms:HUP291 Publication
Ordered Locus Names:At5g15120Imported
ORF Names:F8M21.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G15120.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293Plant cysteine oxidase 1PRO_0000432449Add
BLAST

Proteomic databases

PaxDbiQ9LXG9.
PRIDEiQ9LXG9.

PTM databases

iPTMnetiQ9LXG9.

Expressioni

Developmental stagei

Expressed throughout development, with the highest expression in mature siliques and during seed germination.1 Publication

Inductioni

Up-regulated by hypoxia (PubMed:20097791). Up-regulated by the ERF-VII transcription factor RAP2-12 during hypoxia (PubMed:24599061).2 Publications

Gene expression databases

GenevisibleiQ9LXG9. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G15120.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LXG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cysteine dioxygenase family.Curated

Phylogenomic databases

eggNOGiKOG4281. Eukaryota.
ENOG4111Y3H. LUCA.
HOGENOMiHOG000240285.
KOiK10712.
OMAiCKETFAN.
OrthoDBiEOG09360JAX.
PhylomeDBiQ9LXG9.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012864. PCO/ADO.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07847. DUF1637. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9LXG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFEMKPEKE VLELISSKNQ CKSNPNSVKK KNKNKNKKMM MTWRRKKIDS
60 70 80 90 100
PADGITAVRR LFNTCKEVFS NGGPGVIPSE DKIQQLREIL DDMKPEDVGL
110 120 130 140 150
TPTMPYFRPN SGVEARSSPP ITYLHLHQCD QFSIGIFCLP PSGVIPLHNH
160 170 180 190 200
PGMTVFSKLL FGTMHIKSYD WVVDAPMRDS KTRLAKLKVD STFTAPCNAS
210 220 230 240 250
ILYPEDGGNM HRFTAITACA VLDVLGPPYC NPEGRHCTYF LEFPLDKLSS
260 270 280 290
EDDDVLSSEE EKEGYAWLQE RDDNPEDHTN VVGALYRGPK VED
Length:293
Mass (Da):33,014
Last modified:October 1, 2000 - v1
Checksum:iEF8037CEABA5E9AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353993 Genomic DNA. Translation: CAB89322.1.
CP002688 Genomic DNA. Translation: AED92119.1.
BT008337 mRNA. Translation: AAP37696.1.
AK228354 mRNA. Translation: BAF00293.1.
PIRiT49947.
RefSeqiNP_197016.1. NM_121516.3.
UniGeneiAt.43329.

Genome annotation databases

EnsemblPlantsiAT5G15120.1; AT5G15120.1; AT5G15120.
GeneIDi831364.
GrameneiAT5G15120.1; AT5G15120.1; AT5G15120.
KEGGiath:AT5G15120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353993 Genomic DNA. Translation: CAB89322.1.
CP002688 Genomic DNA. Translation: AED92119.1.
BT008337 mRNA. Translation: AAP37696.1.
AK228354 mRNA. Translation: BAF00293.1.
PIRiT49947.
RefSeqiNP_197016.1. NM_121516.3.
UniGeneiAt.43329.

3D structure databases

ProteinModelPortaliQ9LXG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G15120.1.

PTM databases

iPTMnetiQ9LXG9.

Proteomic databases

PaxDbiQ9LXG9.
PRIDEiQ9LXG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G15120.1; AT5G15120.1; AT5G15120.
GeneIDi831364.
GrameneiAT5G15120.1; AT5G15120.1; AT5G15120.
KEGGiath:AT5G15120.

Organism-specific databases

TAIRiAT5G15120.

Phylogenomic databases

eggNOGiKOG4281. Eukaryota.
ENOG4111Y3H. LUCA.
HOGENOMiHOG000240285.
KOiK10712.
OMAiCKETFAN.
OrthoDBiEOG09360JAX.
PhylomeDBiQ9LXG9.

Enzyme and pathway databases

ReactomeiR-ATH-1614558. Degradation of cysteine and homocysteine.

Miscellaneous databases

PROiQ9LXG9.

Gene expression databases

GenevisibleiQ9LXG9. AT.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012864. PCO/ADO.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07847. DUF1637. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPCO1_ARATH
AccessioniPrimary (citable) accession number: Q9LXG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 2015
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.