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Q9LXE5

- DRM1_ARATH

UniProt

Q9LXE5 - DRM1_ARATH

Protein

DNA (cytosine-5)-methyltransferase DRM1

Gene

DRM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.3 Publications

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: TAIR
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. C-5 methylation of cytosine Source: GOC
    2. defense response to fungus Source: TAIR
    3. DNA methylation Source: TAIR
    4. gene silencing Source: UniProtKB
    5. histone H3-K9 methylation Source: TAIR
    6. methylation-dependent chromatin silencing Source: TAIR

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT5G15380-MONOMER.

    Protein family/group databases

    REBASEi15559. M.AthDRM1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase DRM1 (EC:2.1.1.37)
    Alternative name(s):
    Protein DOMAINS REARRANGED METHYLASE 1
    Gene namesi
    Name:DRM1
    Ordered Locus Names:At5g15380/At5g15370
    ORF Names:F8M21.270/F8M21.260
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G15380.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 624624DNA (cytosine-5)-methyltransferase DRM1PRO_0000381941Add
    BLAST

    Proteomic databases

    PaxDbiQ9LXE5.
    PRIDEiQ9LXE5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LXE5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G15380.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXE5.
    SMRiQ9LXE5. Positions 498-618.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 14942UBA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini188 – 23144UBA 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini291 – 622332SAM-dependent MTase DRM-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. DRM-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase DRM-type domain.PROSITE-ProRule annotation
    Contains 2 UBA domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG70699.
    HOGENOMiHOG000030355.
    InParanoidiQ9LXE5.
    OMAiIERCGED.
    PhylomeDBiQ9LXE5.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR001525. C5_MeTfrase.
    IPR025822. C5_MeTrfase_DMR.
    IPR029063. SAM-dependent_MTases-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view]
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS51680. SAM_MT_DRM. 1 hit.
    PS50030. UBA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LXE5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVMSHIFLIS QIQEVEHGDS DDVNWNTDDD ELAIDNFQFS PSPVHISATS    50
    PNSIQNRISD ETVASFVEMG FSTQMIARAI EETAGANMEP MMILETLFNY 100
    SASTEASSSK SKVINHFIAM GFPEEHVIKA MQEHGDEDVG EITNALLTYA 150
    EVDKLRESED MNININDDDD DNLYSLSSDD EEDELNNSSN EDRILQALIK 200
    MGYLREDAAI AIERCGEDAS MEEVVDFICA AQMARQFDEI YAEPDKKELM 250
    NNNKKRRTYT ETPRKPNTDQ LISLPKEMIG FGVPNHPGLM MHRPVPIPDI 300
    ARGPPFFYYE NVAMTPKGVW AKISSHLYDI VPEFVDSKHF CAAARKRGYI 350
    HNLPIQNRFQ IQPPQHNTIQ EAFPLTKRWW PSWDGRTKLN CLLTCIASSR 400
    LTEKIREALE RYDGETPLDV QKWVMYECKK WNLVWVGKNK LAPLDADEME 450
    KLLGFPRDHT RGGGISTTDR YKSLGNSFQV DTVAYHLSVL KPLFPNGINV 500
    LSLFTGIGGG EVALHRLQIK MNVVVSVEIS DANRNILRSF WEQTNQKGIL 550
    REFKDVQKLD DNTIERLMDE YGGFDLVIGG SPCNNLAGGN RHHRVGLGGE 600
    HSSLFFDYCR ILEAVRRKAR HMRR 624
    Length:624
    Mass (Da):70,918
    Last modified:September 1, 2009 - v2
    Checksum:i9B3BBE10CAE68AB7
    GO

    Sequence cautioni

    The sequence CAB89347.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g15370 and At5g15380.
    The sequence CAB89348.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g15370 and At5g15380.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353993 Genomic DNA. Translation: CAB89347.1. Sequence problems.
    AL353993 Genomic DNA. Translation: CAB89348.1. Sequence problems.
    CP002688 Genomic DNA. Translation: AED92154.1.
    AY530748 Genomic DNA. Translation: AAS45433.1.
    PIRiT49972.
    T49973.
    RefSeqiNP_197042.2. NM_121542.2.
    UniGeneiAt.50458.

    Genome annotation databases

    EnsemblPlantsiAT5G15380.1; AT5G15380.1; AT5G15380.
    GeneIDi831390.
    KEGGiath:AT5G15380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL353993 Genomic DNA. Translation: CAB89347.1 . Sequence problems.
    AL353993 Genomic DNA. Translation: CAB89348.1 . Sequence problems.
    CP002688 Genomic DNA. Translation: AED92154.1 .
    AY530748 Genomic DNA. Translation: AAS45433.1 .
    PIRi T49972.
    T49973.
    RefSeqi NP_197042.2. NM_121542.2.
    UniGenei At.50458.

    3D structure databases

    ProteinModelPortali Q9LXE5.
    SMRi Q9LXE5. Positions 498-618.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G15380.1-P.

    Protein family/group databases

    REBASEi 15559. M.AthDRM1.

    Proteomic databases

    PaxDbi Q9LXE5.
    PRIDEi Q9LXE5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G15380.1 ; AT5G15380.1 ; AT5G15380 .
    GeneIDi 831390.
    KEGGi ath:AT5G15380.

    Organism-specific databases

    TAIRi AT5G15380.

    Phylogenomic databases

    eggNOGi NOG70699.
    HOGENOMi HOG000030355.
    InParanoidi Q9LXE5.
    OMAi IERCGED.
    PhylomeDBi Q9LXE5.

    Enzyme and pathway databases

    BioCyci ARA:AT5G15380-MONOMER.

    Gene expression databases

    Genevestigatori Q9LXE5.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR001525. C5_MeTfrase.
    IPR025822. C5_MeTrfase_DMR.
    IPR029063. SAM-dependent_MTases-like.
    IPR015940. UBA/transl_elong_EF1B_N_euk.
    [Graphical view ]
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS51680. SAM_MT_DRM. 1 hit.
    PS50030. UBA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Efficient discovery of DNA polymorphisms in natural populations by Ecotilling."
      Comai L., Young K., Till B.J., Reynolds S.H., Greene E.A., Codomo C.A., Enns L.C., Johnson J.E., Burtner C., Odden A.R., Henikoff S.
      Plant J. 37:778-786(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-570.
      Strain: cv. Columbia.
    4. "Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes."
      Cao X., Jacobsen S.E.
      Proc. Natl. Acad. Sci. U.S.A. 99:16491-16498(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing."
      Cao X., Jacobsen S.E.
      Curr. Biol. 12:1138-1144(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation."
      Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E.
      Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDRM1_ARATH
    AccessioniPrimary (citable) accession number: Q9LXE5
    Secondary accession number(s): Q6QPM0, Q9LXE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3