Q9LXE5 (DRM1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA (cytosine-5)-methyltransferase DRM1
EC=2.1.1.37

Alternative name(s):
Protein DOMAINS REARRANGED METHYLASE 1

Gene names

Name:	DRM1
Ordered Locus Names:	At5g15380
ORF Names:	F8M21.260, F8M21.270

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation. Ref.4 Ref.5 Ref.6
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subcellular location	Nucleus Potential.
Miscellaneous	DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.
Sequence similarities	Contains 2 UBA domains.
Sequence caution	The sequence CAB89347.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes. The sequence CAB89348.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to fungus Inferred from genetic interaction PubMed 22242006. Source: TAIR histone H3-K9 methylation Inferred from genetic interaction PubMed 17239600. Source: TAIR methylation-dependent chromatin silencing Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from mutant phenotype Ref.4. Source: TAIR DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	DNA (cytosine-5)-methyltransferase DRM1		PRO_0000381941
Regions
	Domain	108 – 149	42	UBA 1
	Domain	188 – 231	44	UBA 2

Sequences

	Sequence		Length	Mass (Da)
	Q9LXE5 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 9B3BBE10CAE68AB7	FASTA	624	70,918
	10 20 30 40 50 60 MVMSHIFLIS QIQEVEHGDS DDVNWNTDDD ELAIDNFQFS PSPVHISATS PNSIQNRISD 70 80 90 100 110 120 ETVASFVEMG FSTQMIARAI EETAGANMEP MMILETLFNY SASTEASSSK SKVINHFIAM 130 140 150 160 170 180 GFPEEHVIKA MQEHGDEDVG EITNALLTYA EVDKLRESED MNININDDDD DNLYSLSSDD 190 200 210 220 230 240 EEDELNNSSN EDRILQALIK MGYLREDAAI AIERCGEDAS MEEVVDFICA AQMARQFDEI 250 260 270 280 290 300 YAEPDKKELM NNNKKRRTYT ETPRKPNTDQ LISLPKEMIG FGVPNHPGLM MHRPVPIPDI 310 320 330 340 350 360 ARGPPFFYYE NVAMTPKGVW AKISSHLYDI VPEFVDSKHF CAAARKRGYI HNLPIQNRFQ 370 380 390 400 410 420 IQPPQHNTIQ EAFPLTKRWW PSWDGRTKLN CLLTCIASSR LTEKIREALE RYDGETPLDV 430 440 450 460 470 480 QKWVMYECKK WNLVWVGKNK LAPLDADEME KLLGFPRDHT RGGGISTTDR YKSLGNSFQV 490 500 510 520 530 540 DTVAYHLSVL KPLFPNGINV LSLFTGIGGG EVALHRLQIK MNVVVSVEIS DANRNILRSF 550 560 570 580 590 600 WEQTNQKGIL REFKDVQKLD DNTIERLMDE YGGFDLVIGG SPCNNLAGGN RHHRVGLGGE 610 620 HSSLFFDYCR ILEAVRRKAR HMRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Efficient discovery of DNA polymorphisms in natural populations by Ecotilling." Comai L., Young K., Till B.J., Reynolds S.H., Greene E.A., Codomo C.A., Enns L.C., Johnson J.E., Burtner C., Odden A.R., Henikoff S. Plant J. 37:778-786(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-570. Strain: cv. Columbia.
[4]	"Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes." Cao X., Jacobsen S.E. Proc. Natl. Acad. Sci. U.S.A. 99:16491-16498(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing." Cao X., Jacobsen S.E. Curr. Biol. 12:1138-1144(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation." Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E. Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL353993 Genomic DNA. Translation: CAB89347.1. Sequence problems. AL353993 Genomic DNA. Translation: CAB89348.1. Sequence problems. CP002688 Genomic DNA. Translation: AED92154.1. AY530748 Genomic DNA. Translation: AAS45433.1.
IPI	IPI00518145.
PIR	T49972. T49973.
RefSeq	NP_197042.2. NM_121542.2.
UniGene	At.50458.
3D structure databases
ProteinModelPortal	Q9LXE5.
SMR	Q9LXE5. Positions 432-490, 498-618.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G15380.1-P.
Protein family/group databases
REBASE	4601. M.AthDRM2.
Proteomic databases
PaxDb	Q9LXE5.
PRIDE	Q9LXE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G15380.1; AT5G15380.1; AT5G15380.
GeneID	831390.
KEGG	ath:AT5G15380.
Organism-specific databases
TAIR	At5g15380.
Phylogenomic databases
eggNOG	NOG70699.
HOGENOM	HOG000030355.
InParanoid	Q9LXE5.
OMA	DYCRILE.
PhylomeDB	Q9LXE5.
ProtClustDB	CLSN2679951.
Gene expression databases
Genevestigator	Q9LXE5.
Family and domain databases
InterPro	IPR001525. C5_MeTfrase. IPR025822. C5_MeTrfase_DMR. IPR015940. UBA/transl_elong_EF1B_N_euk. [Graphical view]
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PROSITE	PS00094. C5_MTASE_1. False negative. PS00095. C5_MTASE_2. False negative. PS50030. UBA. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRM1_ARATH

Accession

Primary (citable) accession number: Q9LXE5
Secondary accession number(s): Q6QPM0, Q9LXE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program