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Q9LXE5

- DRM1_ARATH

UniProt

Q9LXE5 - DRM1_ARATH

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Protein
DNA (cytosine-5)-methyltransferase DRM1
Gene
DRM1, At5g15380/At5g15370, F8M21.270/F8M21.260
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation.3 Publications

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: TAIR
  2. DNA binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. C-5 methylation of cytosine Source: GOC
  2. DNA methylation Source: TAIR
  3. defense response to fungus Source: TAIR
  4. gene silencing Source: UniProtKB
  5. histone H3-K9 methylation Source: TAIR
  6. methylation-dependent chromatin silencing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARA:AT5G15380-MONOMER.

Protein family/group databases

REBASEi15559. M.AthDRM1.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase DRM1 (EC:2.1.1.37)
Alternative name(s):
Protein DOMAINS REARRANGED METHYLASE 1
Gene namesi
Name:DRM1
Ordered Locus Names:At5g15380/At5g15370
ORF Names:F8M21.270/F8M21.260
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G15380.

Subcellular locationi

Nucleus Reviewed prediction

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624DNA (cytosine-5)-methyltransferase DRM1
PRO_0000381941Add
BLAST

Proteomic databases

PaxDbiQ9LXE5.
PRIDEiQ9LXE5.

Expressioni

Gene expression databases

GenevestigatoriQ9LXE5.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G15380.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LXE5.
SMRiQ9LXE5. Positions 498-618.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 14942UBA 1
Add
BLAST
Domaini188 – 23144UBA 2
Add
BLAST
Domaini291 – 622332SAM-dependent MTase DRM-type
Add
BLAST

Sequence similaritiesi

Contains 2 UBA domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG70699.
HOGENOMiHOG000030355.
InParanoidiQ9LXE5.
OMAiIERCGED.
PhylomeDBiQ9LXE5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR001525. C5_MeTfrase.
IPR025822. C5_MeTrfase_DMR.
IPR029063. SAM-dependent_MTases-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51680. SAM_MT_DRM. 1 hit.
PS50030. UBA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LXE5-1 [UniParc]FASTAAdd to Basket

« Hide

MVMSHIFLIS QIQEVEHGDS DDVNWNTDDD ELAIDNFQFS PSPVHISATS    50
PNSIQNRISD ETVASFVEMG FSTQMIARAI EETAGANMEP MMILETLFNY 100
SASTEASSSK SKVINHFIAM GFPEEHVIKA MQEHGDEDVG EITNALLTYA 150
EVDKLRESED MNININDDDD DNLYSLSSDD EEDELNNSSN EDRILQALIK 200
MGYLREDAAI AIERCGEDAS MEEVVDFICA AQMARQFDEI YAEPDKKELM 250
NNNKKRRTYT ETPRKPNTDQ LISLPKEMIG FGVPNHPGLM MHRPVPIPDI 300
ARGPPFFYYE NVAMTPKGVW AKISSHLYDI VPEFVDSKHF CAAARKRGYI 350
HNLPIQNRFQ IQPPQHNTIQ EAFPLTKRWW PSWDGRTKLN CLLTCIASSR 400
LTEKIREALE RYDGETPLDV QKWVMYECKK WNLVWVGKNK LAPLDADEME 450
KLLGFPRDHT RGGGISTTDR YKSLGNSFQV DTVAYHLSVL KPLFPNGINV 500
LSLFTGIGGG EVALHRLQIK MNVVVSVEIS DANRNILRSF WEQTNQKGIL 550
REFKDVQKLD DNTIERLMDE YGGFDLVIGG SPCNNLAGGN RHHRVGLGGE 600
HSSLFFDYCR ILEAVRRKAR HMRR 624
Length:624
Mass (Da):70,918
Last modified:September 1, 2009 - v2
Checksum:i9B3BBE10CAE68AB7
GO

Sequence cautioni

The sequence CAB89347.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g15370 and At5g15380.
The sequence CAB89348.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g15370 and At5g15380.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL353993 Genomic DNA. Translation: CAB89347.1. Sequence problems.
AL353993 Genomic DNA. Translation: CAB89348.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92154.1.
AY530748 Genomic DNA. Translation: AAS45433.1.
PIRiT49972.
T49973.
RefSeqiNP_197042.2. NM_121542.2.
UniGeneiAt.50458.

Genome annotation databases

EnsemblPlantsiAT5G15380.1; AT5G15380.1; AT5G15380.
GeneIDi831390.
KEGGiath:AT5G15380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL353993 Genomic DNA. Translation: CAB89347.1 . Sequence problems.
AL353993 Genomic DNA. Translation: CAB89348.1 . Sequence problems.
CP002688 Genomic DNA. Translation: AED92154.1 .
AY530748 Genomic DNA. Translation: AAS45433.1 .
PIRi T49972.
T49973.
RefSeqi NP_197042.2. NM_121542.2.
UniGenei At.50458.

3D structure databases

ProteinModelPortali Q9LXE5.
SMRi Q9LXE5. Positions 498-618.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT5G15380.1-P.

Protein family/group databases

REBASEi 15559. M.AthDRM1.

Proteomic databases

PaxDbi Q9LXE5.
PRIDEi Q9LXE5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G15380.1 ; AT5G15380.1 ; AT5G15380 .
GeneIDi 831390.
KEGGi ath:AT5G15380.

Organism-specific databases

TAIRi AT5G15380.

Phylogenomic databases

eggNOGi NOG70699.
HOGENOMi HOG000030355.
InParanoidi Q9LXE5.
OMAi IERCGED.
PhylomeDBi Q9LXE5.

Enzyme and pathway databases

BioCyci ARA:AT5G15380-MONOMER.

Gene expression databases

Genevestigatori Q9LXE5.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR001525. C5_MeTfrase.
IPR025822. C5_MeTrfase_DMR.
IPR029063. SAM-dependent_MTases-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view ]
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS51680. SAM_MT_DRM. 1 hit.
PS50030. UBA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Efficient discovery of DNA polymorphisms in natural populations by Ecotilling."
    Comai L., Young K., Till B.J., Reynolds S.H., Greene E.A., Codomo C.A., Enns L.C., Johnson J.E., Burtner C., Odden A.R., Henikoff S.
    Plant J. 37:778-786(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-570.
    Strain: cv. Columbia.
  4. "Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes."
    Cao X., Jacobsen S.E.
    Proc. Natl. Acad. Sci. U.S.A. 99:16491-16498(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing."
    Cao X., Jacobsen S.E.
    Curr. Biol. 12:1138-1144(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation."
    Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E.
    Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDRM1_ARATH
AccessioniPrimary (citable) accession number: Q9LXE5
Secondary accession number(s): Q6QPM0, Q9LXE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: July 9, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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