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Q9LXE5 (DRM1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA (cytosine-5)-methyltransferase DRM1

EC=2.1.1.37
Alternative name(s):
Protein DOMAINS REARRANGED METHYLASE 1
Gene names
Name:DRM1
Ordered Locus Names:At5g15380/At5g15370
ORF Names:F8M21.270/F8M21.260
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length624 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation. Ref.4 Ref.5 Ref.6

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subcellular location

Nucleus Potential.

Miscellaneous

DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. DRM-methyltransferase family.

Contains 1 SAM-dependent MTase DRM-type domain.

Contains 2 UBA domains.

Sequence caution

The sequence CAB89347.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g15370 and At5g15380.

The sequence CAB89348.1 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g15370 and At5g15380.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 624624DNA (cytosine-5)-methyltransferase DRM1
PRO_0000381941

Regions

Domain108 – 14942UBA 1
Domain188 – 23144UBA 2
Domain291 – 622332SAM-dependent MTase DRM-type

Sequences

Sequence LengthMass (Da)Tools
Q9LXE5 [UniParc].

Last modified September 1, 2009. Version 2.
Checksum: 9B3BBE10CAE68AB7

FASTA62470,918
        10         20         30         40         50         60 
MVMSHIFLIS QIQEVEHGDS DDVNWNTDDD ELAIDNFQFS PSPVHISATS PNSIQNRISD 

        70         80         90        100        110        120 
ETVASFVEMG FSTQMIARAI EETAGANMEP MMILETLFNY SASTEASSSK SKVINHFIAM 

       130        140        150        160        170        180 
GFPEEHVIKA MQEHGDEDVG EITNALLTYA EVDKLRESED MNININDDDD DNLYSLSSDD 

       190        200        210        220        230        240 
EEDELNNSSN EDRILQALIK MGYLREDAAI AIERCGEDAS MEEVVDFICA AQMARQFDEI 

       250        260        270        280        290        300 
YAEPDKKELM NNNKKRRTYT ETPRKPNTDQ LISLPKEMIG FGVPNHPGLM MHRPVPIPDI 

       310        320        330        340        350        360 
ARGPPFFYYE NVAMTPKGVW AKISSHLYDI VPEFVDSKHF CAAARKRGYI HNLPIQNRFQ 

       370        380        390        400        410        420 
IQPPQHNTIQ EAFPLTKRWW PSWDGRTKLN CLLTCIASSR LTEKIREALE RYDGETPLDV 

       430        440        450        460        470        480 
QKWVMYECKK WNLVWVGKNK LAPLDADEME KLLGFPRDHT RGGGISTTDR YKSLGNSFQV 

       490        500        510        520        530        540 
DTVAYHLSVL KPLFPNGINV LSLFTGIGGG EVALHRLQIK MNVVVSVEIS DANRNILRSF 

       550        560        570        580        590        600 
WEQTNQKGIL REFKDVQKLD DNTIERLMDE YGGFDLVIGG SPCNNLAGGN RHHRVGLGGE 

       610        620 
HSSLFFDYCR ILEAVRRKAR HMRR 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Efficient discovery of DNA polymorphisms in natural populations by Ecotilling."
Comai L., Young K., Till B.J., Reynolds S.H., Greene E.A., Codomo C.A., Enns L.C., Johnson J.E., Burtner C., Odden A.R., Henikoff S.
Plant J. 37:778-786(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-570.
Strain: cv. Columbia.
[4]"Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes."
Cao X., Jacobsen S.E.
Proc. Natl. Acad. Sci. U.S.A. 99:16491-16498(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Role of the arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing."
Cao X., Jacobsen S.E.
Curr. Biol. 12:1138-1144(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation."
Cao X., Aufsatz W., Zilberman D., Mette M.F., Huang M.S., Matzke M., Jacobsen S.E.
Curr. Biol. 13:2212-2217(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL353993 Genomic DNA. Translation: CAB89347.1. Sequence problems.
AL353993 Genomic DNA. Translation: CAB89348.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED92154.1.
AY530748 Genomic DNA. Translation: AAS45433.1.
PIRT49972.
T49973.
RefSeqNP_197042.2. NM_121542.2.
UniGeneAt.50458.

3D structure databases

ProteinModelPortalQ9LXE5.
SMRQ9LXE5. Positions 61-150, 303-495, 498-618.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G15380.1-P.

Protein family/group databases

REBASE15559. M.AthDRM1.

Proteomic databases

PaxDbQ9LXE5.
PRIDEQ9LXE5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G15380.1; AT5G15380.1; AT5G15380.
GeneID831390.
KEGGath:AT5G15380.

Organism-specific databases

TAIRAT5G15380.

Phylogenomic databases

eggNOGNOG70699.
HOGENOMHOG000030355.
InParanoidQ9LXE5.
OMAIERCGED.
PhylomeDBQ9LXE5.
ProtClustDBCLSN2679951.

Enzyme and pathway databases

BioCycARA:AT5G15380-MONOMER.

Gene expression databases

GenevestigatorQ9LXE5.

Family and domain databases

InterProIPR001525. C5_MeTfrase.
IPR025822. C5_MeTrfase_DMR.
IPR015940. UBA/transl_elong_EF1B_N_euk.
[Graphical view]
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PROSITEPS51680. SAM_MT_DRM. 1 hit.
PS50030. UBA. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDRM1_ARATH
AccessionPrimary (citable) accession number: Q9LXE5
Secondary accession number(s): Q6QPM0, Q9LXE6
Entry history
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: April 16, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names