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Protein

Probable pectinesterase/pectinesterase inhibitor 51

Gene

PME51

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Pectinesterase (PMEPCRF), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At3g43380), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (At5g26810), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (At3g49220), Probable pectinesterase 29 (PME29), Pectinesterase (AXX17_At4g38040), Pectinesterase (At5g20860), Probable pectinesterase 8 (PME8), Uncharacterized protein (AXX17_At5g27870), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase (AXX17_At3g53530), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Probable pectinesterase 67 (PME67), Probable pectinesterase 56 (PME56), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Uncharacterized protein (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (PME44), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (At4g15980), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Uncharacterized protein (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Pectate lyase (At3g27400), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Pectate lyase (At5g04310), Probable pectate lyase 22 (At5g63180), Pectate lyase (At5g09280), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase, Pectate lyase, Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (At3g09540), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (AXX17_At4g28670), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase, Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei317Substrate; for pectinesterase activityBy similarity1
Binding sitei347Substrate; for pectinesterase activityBy similarity1
Sitei369Transition state stabilizerBy similarity1
Active sitei370Proton donor; for pectinesterase activityBy similarity1
Active sitei391Nucleophile; for pectinesterase activityBy similarity1
Binding sitei467Substrate; for pectinesterase activityBy similarity1
Binding sitei469Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 51
Including the following 2 domains:
Pectinesterase inhibitor 51
Alternative name(s):
Pectin methylesterase inhibitor 51
Pectinesterase 51 (EC:3.1.1.11)
Short name:
PE 51
Alternative name(s):
Pectin methylesterase 51
Short name:
AtPME51
Gene namesi
Name:PME51
Synonyms:ARATH51
Ordered Locus Names:At5g09760
ORF Names:F17I14.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G09760
TAIRilocus:2144806 AT5G09760

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000037169921 – 551Probable pectinesterase/pectinesterase inhibitor 51Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi164N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi384 ↔ 404By similarity
Glycosylationi445N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9LXD9
PRIDEiQ9LXD9

Expressioni

Tissue specificityi

Expressed in siliques.1 Publication

Developmental stagei

Expressed throughout silique development.1 Publication

Gene expression databases

ExpressionAtlasiQ9LXD9 baseline and differential
GenevisibleiQ9LXD9 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G09760.1

Structurei

3D structure databases

ProteinModelPortaliQ9LXD9
SMRiQ9LXD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 189Pectinesterase inhibitor 51Add BLAST151
Regioni240 – 544Pectinesterase 51Add BLAST305

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi23 – 27Poly-His5

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFZX Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9LXD9
OMAiTSRWAPP
OrthoDBiEOG0936077N
PhylomeDBiQ9LXD9

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LXD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSILILLFS LFLFSSPSSS SRHHHHHNSG DTSPVNPSSS LAAQIRLACN
60 70 80 90 100
ATRYPDQCVS SLSEQGRVPP DPKPIQIIHS AISFSFQNLK TAQSKIKSIV
110 120 130 140 150
DSSVGNLNRT NAANTCLQLL TYSEHRTQST DQALTRGKIK DARAWMSAAL
160 170 180 190 200
VYQYDSWSAL KYVNDTSQVG ETMSFLDGLI HVTSNALSMM VSYDNFGDNV
210 220 230 240 250
ASWTYPATER DGFWEKTGPG LGLDPSTGLN LGFPSGLKED VTVCKDGKCG
260 270 280 290 300
YKTVQDAVNA APEDNGMRKF VIKISEGVYE ENVIVPFEKK NVVFIGDGMG
310 320 330 340 350
KTVITGSLNA GMPGITTYNT ATVGVVGDGF MARDLTFQNT AGPDAHQAVA
360 370 380 390 400
FRSDSDFSLI ENCEFLGNQD TLYAHGLRQF YKNCRIQGNV DFIFGNSAAV
410 420 430 440 450
FQDCEILIAP RQINPEKGEK NAVTAQGRID PSQSTGFVFL NCLINGTEEY
460 470 480 490 500
MKLFKANPKV HKNFLGRPWK DYSRTVFIGC NLEALITPDG WLPWSGDFAL
510 520 530 540 550
KTLYYGESKN TGPGSDRSQR VSWSSQIPDE HVHVYSVANF IQADEWASMS

A
Length:551
Mass (Da):60,439
Last modified:October 1, 2000 - v1
Checksum:i0367BA2E4E1CE789
GO

Sequence cautioni

The sequence BAB09534 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020752 Genomic DNA Translation: BAB09534.1 Different initiation.
AL353994 Genomic DNA Translation: CAB89354.1
CP002688 Genomic DNA Translation: AED91443.1
AY070093 mRNA Translation: AAL49830.1
BT006059 mRNA Translation: AAP04044.1
PIRiT49922
RefSeqiNP_001331158.1, NM_001343067.1
NP_196538.1, NM_121013.3
UniGeneiAt.28396

Genome annotation databases

EnsemblPlantsiAT5G09760.1; AT5G09760.1; AT5G09760
AT5G09760.2; AT5G09760.2; AT5G09760
GeneIDi830836
GrameneiAT5G09760.1; AT5G09760.1; AT5G09760
AT5G09760.2; AT5G09760.2; AT5G09760
KEGGiath:AT5G09760

Similar proteinsi

Entry informationi

Entry nameiPME51_ARATH
AccessioniPrimary (citable) accession number: Q9LXD9
Secondary accession number(s): Q9FXW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health