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Protein

Histone acetyltransferase of the MYST family 2

Gene

HAM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone acetyltransferase which may be involved in transcriptional activation. Acetylates 'Lys-5' of histone H4 (H4K5ac) (PubMed:16648464, PubMed:17877703). Essential for gametophyte development (PubMed:19040736). Negative regulator of flowering controlling the H4K5ac levels in the FLC chromatin (PubMed:23273925).4 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei345Proton donor/acceptorBy similarity1
Binding sitei349Acetyl-CoABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri202 – 227C2HC MYST-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

  • histone acetyltransferase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to UV-B Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.1.48. 399.
ReactomeiR-ATH-2559586. DNA Damage/Telomere Stress Induced Senescence.
R-ATH-3214847. HATs acetylate histones.
R-ATH-5693548. Sensing of DNA Double Strand Breaks.
R-ATH-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase of the MYST family 21 Publication (EC:2.3.1.481 Publication)
Alternative name(s):
Histone acetyltransferase of the GNAT/MYST superfamily 51 Publication
MYST-like histone acetyltransferase 21 Publication
Gene namesi
Name:HAM21 Publication
Synonyms:HAG51 Publication
Ordered Locus Names:At5g09740Imported
ORF Names:F17I14.70Imported, MTH16.20Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G09740.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, due to the redundancy with HAM1. Ham1 and ham2 double mutants are lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002384651 – 445Histone acetyltransferase of the MYST family 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei269N6-acetyllysine; by autocatalysisBy similarity1

Post-translational modificationi

Autoacetylation at Lys-269 is required for proper function.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9LXD7.

Expressioni

Tissue specificityi

Expressed in cotyledons, leaves, stems, roots and, at higher levels in developing flowers, particularly in the anthers and gynoecia (PubMed:19040736). Constitutively expressed in all tissues, predominantly in shoot apical meristem (PubMed:23273925).2 Publications

Inductioni

Up-regulated upon UV-B exposure.1 Publication

Gene expression databases

ExpressionAtlasiQ9LXD7. baseline and differential.
GenevisibleiQ9LXD7. AT.

Interactioni

Subunit structurei

Interacts with MRG1 and MRG2.1 Publication

Protein-protein interaction databases

BioGridi16112. 1 interactor.
STRINGi3702.AT5G09740.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LXD7.
SMRiQ9LXD7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 133ChromoAdd BLAST60
Domaini169 – 440MYST-type HATPROSITE-ProRule annotationAdd BLAST272

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni312 – 314Acetyl-CoA bindingBy similarity3
Regioni319 – 325Acetyl-CoA bindingBy similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi14 – 40Pro-richAdd BLAST27

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated
Contains 1 C2HC MYST-type zinc finger.PROSITE-ProRule annotation
Contains 1 chromo domain.Curated
Contains 1 MYST-type HAT (histone acetyltransferase) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri202 – 227C2HC MYST-typePROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2747. Eukaryota.
COG5027. LUCA.
HOGENOMiHOG000182457.
InParanoidiQ9LXD7.
KOiK11308.
OMAiKIDETHI.
OrthoDBiEOG09360B38.
PhylomeDBiQ9LXD7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo/shadow_dom.
IPR016197. Chromodomain-like.
IPR002717. HAT_MYST-type.
IPR025995. Tudor-knot.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LXD7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSANTETN GNAPPPSSNQ KPPATNGVDG SHPPPPPLTP DQAIIESDPS
60 70 80 90 100
KKRKMGMLPL EVGTRVMCRW RDGKHHPVKV IERRRIHNGG QNDYEYYVHY
110 120 130 140 150
TEFNRRLDEW TQLDQLDLDS VECAVDEKVE DKVTSLKMTR HQKRKIDETH
160 170 180 190 200
IEGHEELDAA SLREHEEFTK VKNISTIELG KYEIETWYFS PFPPEYNDCV
210 220 230 240 250
KLFFCEFCLN FMKRKEQLQR HMRKCDLKHP PGDEIYRSGT LSMFEVDGKK
260 270 280 290 300
NKVYAQNLCY LAKLFLDHKT LYYDVDLFLF YVLCECDDRG CHMVGYFSKE
310 320 330 340 350
KHSEEAYNLA CILTLPSYQR KGYGKFLIAF SYELSKKEGK VGTPERPLSD
360 370 380 390 400
LGLLSYRGYW TRVLLEILKK HKGNISIKEL SDVTAIKAED ILSTLQSLEL
410 420 430 440
IQYRKGQHVI CADPKVLDRH LKAAGRGGLD VDASKLIWTP YKDQS
Length:445
Mass (Da):51,367
Last modified:October 1, 2000 - v1
Checksum:i3BA39F07E2E47C33
GO
Isoform 2 (identifier: Q9LXD7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: S → N
     172-249: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):42,007
Checksum:i74D19D94047E699D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129V → L in AAM63720 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018609135S → N in isoform 2. 1 Publication1
Alternative sequenceiVSP_018610172 – 249Missing in isoform 2. 1 PublicationAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353994 Genomic DNA. Translation: CAB89356.1.
AB020752 Genomic DNA. Translation: BAB09532.1.
CP002688 Genomic DNA. Translation: AED91440.1.
CP002688 Genomic DNA. Translation: AED91441.1.
AK117126 mRNA. Translation: BAC41804.1.
AY086663 mRNA. Translation: AAM63720.1.
AB493744 mRNA. Translation: BAH30582.1.
PIRiT49924.
RefSeqiNP_001078558.1. NM_001085089.1.
NP_196536.1. NM_121011.4. [Q9LXD7-1]
UniGeneiAt.32464.

Genome annotation databases

EnsemblPlantsiAT5G09740.1; AT5G09740.1; AT5G09740. [Q9LXD7-1]
AT5G09740.2; AT5G09740.2; AT5G09740.
GeneIDi830834.
GrameneiAT5G09740.1; AT5G09740.1; AT5G09740.
AT5G09740.2; AT5G09740.2; AT5G09740.
KEGGiath:AT5G09740.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353994 Genomic DNA. Translation: CAB89356.1.
AB020752 Genomic DNA. Translation: BAB09532.1.
CP002688 Genomic DNA. Translation: AED91440.1.
CP002688 Genomic DNA. Translation: AED91441.1.
AK117126 mRNA. Translation: BAC41804.1.
AY086663 mRNA. Translation: AAM63720.1.
AB493744 mRNA. Translation: BAH30582.1.
PIRiT49924.
RefSeqiNP_001078558.1. NM_001085089.1.
NP_196536.1. NM_121011.4. [Q9LXD7-1]
UniGeneiAt.32464.

3D structure databases

ProteinModelPortaliQ9LXD7.
SMRiQ9LXD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16112. 1 interactor.
STRINGi3702.AT5G09740.1.

Proteomic databases

PaxDbiQ9LXD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G09740.1; AT5G09740.1; AT5G09740. [Q9LXD7-1]
AT5G09740.2; AT5G09740.2; AT5G09740.
GeneIDi830834.
GrameneiAT5G09740.1; AT5G09740.1; AT5G09740.
AT5G09740.2; AT5G09740.2; AT5G09740.
KEGGiath:AT5G09740.

Organism-specific databases

TAIRiAT5G09740.

Phylogenomic databases

eggNOGiKOG2747. Eukaryota.
COG5027. LUCA.
HOGENOMiHOG000182457.
InParanoidiQ9LXD7.
KOiK11308.
OMAiKIDETHI.
OrthoDBiEOG09360B38.
PhylomeDBiQ9LXD7.

Enzyme and pathway databases

BRENDAi2.3.1.48. 399.
ReactomeiR-ATH-2559586. DNA Damage/Telomere Stress Induced Senescence.
R-ATH-3214847. HATs acetylate histones.
R-ATH-5693548. Sensing of DNA Double Strand Breaks.
R-ATH-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Miscellaneous databases

PROiQ9LXD7.

Gene expression databases

ExpressionAtlasiQ9LXD7. baseline and differential.
GenevisibleiQ9LXD7. AT.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo/shadow_dom.
IPR016197. Chromodomain-like.
IPR002717. HAT_MYST-type.
IPR025995. Tudor-knot.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYST2_ARATH
AccessioniPrimary (citable) accession number: Q9LXD7
Secondary accession number(s): C0SVP1
, F4KE11, Q8GZ97, Q8LCD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Knockdown expression of both HAM1 and HAM2 results in earlier flowering.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.