Q9LXD7 (MYST2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MYST-like histone acetyltransferase 2
EC=2.3.1.48

Gene names

Name:	HAG5
Ordered Locus Names:	At5g09740
ORF Names:	F17I14.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone acetyltransferase which may be involved in transcriptional activation. Acetylates 'Lys-5' of histone H4. Ref.6
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subcellular location	Nucleus Probable.
Post-translational modification	Autoacetylation at Lys-269 is required for proper function By similarity.
Sequence similarities	Belongs to the MYST (SAS/MOZ) family. Contains 1 C2HC-type zinc finger. Contains 1 chromo domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Acyltransferase Chromatin regulator Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from mutant phenotype PubMed 22170978. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to UV-B Inferred from expression pattern PubMed 22170978. Source: TAIR transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 17877703. Source: TAIR
Molecular_function	histone acetyltransferase activity Inferred from direct assay PubMed 17877703. Source: TAIR zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LXD7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LXD7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: S → N
     172-249: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	MYST-like histone acetyltransferase 2		PRO_0000238465
Regions
	Domain	74 – 133	60	Chromo
	Zinc finger	203 – 225	23	C2HC-type
	Region	319 – 325	7	Acetyl-CoA binding By similarity
	Compositional bias	14 – 40	27	Pro-rich
Sites
	Active site	269	1	By similarity
	Active site	311	1	Nucleophile By similarity
	Binding site	314	1	Acetyl-CoA By similarity
	Binding site	349	1	Acetyl-CoA By similarity
Amino acid modifications
	Modified residue	269	1	N6-acetyllysine; by autocatalysis By similarity
Natural variations
	Alternative sequence	135	1	S → N in isoform 2.		VSP_018609
	Alternative sequence	172 – 249	78	Missing in isoform 2.		VSP_018610
Experimental info
	Sequence conflict	129	1	V → L in AAM63720. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3BA39F07E2E47C33	FASTA	445	51,367
	10 20 30 40 50 60 MGSSANTETN GNAPPPSSNQ KPPATNGVDG SHPPPPPLTP DQAIIESDPS KKRKMGMLPL 70 80 90 100 110 120 EVGTRVMCRW RDGKHHPVKV IERRRIHNGG QNDYEYYVHY TEFNRRLDEW TQLDQLDLDS 130 140 150 160 170 180 VECAVDEKVE DKVTSLKMTR HQKRKIDETH IEGHEELDAA SLREHEEFTK VKNISTIELG 190 200 210 220 230 240 KYEIETWYFS PFPPEYNDCV KLFFCEFCLN FMKRKEQLQR HMRKCDLKHP PGDEIYRSGT 250 260 270 280 290 300 LSMFEVDGKK NKVYAQNLCY LAKLFLDHKT LYYDVDLFLF YVLCECDDRG CHMVGYFSKE 310 320 330 340 350 360 KHSEEAYNLA CILTLPSYQR KGYGKFLIAF SYELSKKEGK VGTPERPLSD LGLLSYRGYW 370 380 390 400 410 420 TRVLLEILKK HKGNISIKEL SDVTAIKAED ILSTLQSLEL IQYRKGQHVI CADPKVLDRH 430 440 LKAAGRGGLD VDASKLIWTP YKDQS
	Isoform 2 [UniParc]. Checksum: 74D19D94047E699D	FASTA	367	42,007
	10 20 30 40 50 60 MGSSANTETN GNAPPPSSNQ KPPATNGVDG SHPPPPPLTP DQAIIESDPS KKRKMGMLPL 70 80 90 100 110 120 EVGTRVMCRW RDGKHHPVKV IERRRIHNGG QNDYEYYVHY TEFNRRLDEW TQLDQLDLDS 130 140 150 160 170 180 VECAVDEKVE DKVTNLKMTR HQKRKIDETH IEGHEELDAA SLREHEEFTK VKNKVYAQNL 190 200 210 220 230 240 CYLAKLFLDH KTLYYDVDLF LFYVLCECDD RGCHMVGYFS KEKHSEEAYN LACILTLPSY 250 260 270 280 290 300 QRKGYGKFLI AFSYELSKKE GKVGTPERPL SDLGLLSYRG YWTRVLLEIL KKHKGNISIK 310 320 330 340 350 360 ELSDVTAIKA EDILSTLQSL ELIQYRKGQH VICADPKVLD RHLKAAGRGG LDVDASKLIW TPYKDQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	"Erasure of histone acetylation by Arabidopsis HDA6 mediates large-scale gene silencing in nucleolar dominance." Earley K., Lawrence R.J., Pontes O., Reuther R., Enciso A.J., Silva M., Neves N., Gross M., Viegas W., Pikaard C.S. Genes Dev. 20:1283-1293(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes." Pandey R., Mueller A., Napoli C.A., Selinger D.A., Pikaard C.S., Richards E.J., Bender J., Mount D.W., Jorgensen R.A. Nucleic Acids Res. 30:5036-5055(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL353994 Genomic DNA. Translation: CAB89356.1. AB020752 Genomic DNA. Translation: BAB09532.1. CP002688 Genomic DNA. Translation: AED91440.1. AK117126 mRNA. Translation: BAC41804.1. AY086663 mRNA. Translation: AAM63720.1.
IPI	IPI00518243. IPI00544256.
PIR	T49924.
RefSeq	NP_196536.1. NM_121011.3.
UniGene	At.32464.
3D structure databases
ProteinModelPortal	Q9LXD7.
SMR	Q9LXD7. Positions 172-440.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G09740.1; AT5G09740.1; AT5G09740.
GeneID	830834.
KEGG	ath:AT5G09740.
Organism-specific databases
TAIR	At5g09740.
Phylogenomic databases
eggNOG	COG5027.
HOGENOM	HOG000182457.
InParanoid	Q9LXD7.
KO	K11308.
OMA	IERRRIH.
PhylomeDB	Q9LXD7.
ProtClustDB	PLN00104.
Gene expression databases
ArrayExpress	Q9LXD7.
Genevestigator	Q9LXD7.
GermOnline	AT5G09740. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. IPR002717. MOZ_SAS. IPR025995. Tudor-knot. IPR015880. Znf_C2H2-like. [Graphical view]
Pfam	PF01853. MOZ_SAS. 1 hit. PF11717. Tudor-knot. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit. SSF54160. Chromodomain-like. 1 hit.
PROSITE	PS00598. CHROMO_1. False negative. PS50013. CHROMO_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MYST2_ARATH

Accession

Primary (citable) accession number: Q9LXD7
Secondary accession number(s): Q8GZ97, Q8LCD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program