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Protein

Histone acetyltransferase of the MYST family 2

Gene

HAM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone acetyltransferase which may be involved in transcriptional activation. Acetylates 'Lys-5' of histone H4 (H4K5ac) (PubMed:16648464, PubMed:17877703). Essential for gametophyte development (PubMed:19040736). Negative regulator of flowering controlling the H4K5ac levels in the FLC chromatin (PubMed:23273925).4 Publications

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei345 – 3451Proton donor/acceptorBy similarity
Binding sitei349 – 3491Acetyl-CoABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri203 – 22523C2HC-typeAdd
BLAST

GO - Molecular functioni

  1. histone acetyltransferase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: TAIR
  2. histone acetylation Source: GOC
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. response to UV-B Source: TAIR
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G09740-MONOMER.
BRENDAi2.3.1.48. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase of the MYST family 21 Publication (EC:2.3.1.481 Publication)
Alternative name(s):
Histone acetyltransferase of the GNAT/MYST superfamily 51 Publication
MYST-like histone acetyltransferase 21 Publication
Gene namesi
Name:HAM21 Publication
Synonyms:HAG51 Publication
Ordered Locus Names:At5g09740Imported
ORF Names:F17I14.70Imported, MTH16.20Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G09740.

Subcellular locationi

  1. Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, due to the redundancy with HAM1. Ham1 and ham2 double mutants are lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Histone acetyltransferase of the MYST family 2PRO_0000238465Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei269 – 2691N6-acetyllysine; by autocatalysisBy similarity

Post-translational modificationi

Autoacetylation at Lys-269 is required for proper function.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9LXD7.

Expressioni

Tissue specificityi

Expressed in cotyledons, leaves, stems, roots and, at higher levels in developing flowers, particularly in the anthers and gynoecia (PubMed:19040736). Constitutively expressed in all tissues, predominantly in shoot apical meristem (PubMed:23273925).2 Publications

Inductioni

Up-regulated upon UV-B exposure.1 Publication

Gene expression databases

GenevestigatoriQ9LXD7.

Interactioni

Subunit structurei

Interacts with MRG1 and MRG2.1 Publication

Protein-protein interaction databases

BioGridi16112. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9LXD7.
SMRiQ9LXD7. Positions 172-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 13360ChromoAdd
BLAST
Domaini169 – 440272MYST-type HATAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni312 – 3143Acetyl-CoA bindingBy similarity
Regioni319 – 3257Acetyl-CoA bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 4027Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.Curated
Contains 1 C2HC-type zinc finger.Curated
Contains 1 chromo domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri203 – 22523C2HC-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5027.
HOGENOMiHOG000182457.
InParanoidiQ9LXD7.
KOiK11308.
OMAiLDEWTQL.
PhylomeDBiQ9LXD7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo/shadow_dom.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LXD7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSSANTETN GNAPPPSSNQ KPPATNGVDG SHPPPPPLTP DQAIIESDPS
60 70 80 90 100
KKRKMGMLPL EVGTRVMCRW RDGKHHPVKV IERRRIHNGG QNDYEYYVHY
110 120 130 140 150
TEFNRRLDEW TQLDQLDLDS VECAVDEKVE DKVTSLKMTR HQKRKIDETH
160 170 180 190 200
IEGHEELDAA SLREHEEFTK VKNISTIELG KYEIETWYFS PFPPEYNDCV
210 220 230 240 250
KLFFCEFCLN FMKRKEQLQR HMRKCDLKHP PGDEIYRSGT LSMFEVDGKK
260 270 280 290 300
NKVYAQNLCY LAKLFLDHKT LYYDVDLFLF YVLCECDDRG CHMVGYFSKE
310 320 330 340 350
KHSEEAYNLA CILTLPSYQR KGYGKFLIAF SYELSKKEGK VGTPERPLSD
360 370 380 390 400
LGLLSYRGYW TRVLLEILKK HKGNISIKEL SDVTAIKAED ILSTLQSLEL
410 420 430 440
IQYRKGQHVI CADPKVLDRH LKAAGRGGLD VDASKLIWTP YKDQS
Length:445
Mass (Da):51,367
Last modified:October 1, 2000 - v1
Checksum:i3BA39F07E2E47C33
GO
Isoform 2 (identifier: Q9LXD7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-135: S → N
     172-249: Missing.

Note: No experimental confirmation available.

Show »
Length:367
Mass (Da):42,007
Checksum:i74D19D94047E699D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291V → L in AAM63720 (Ref. 5) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei135 – 1351S → N in isoform 2. 1 PublicationVSP_018609
Alternative sequencei172 – 24978Missing in isoform 2. 1 PublicationVSP_018610Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353994 Genomic DNA. Translation: CAB89356.1.
AB020752 Genomic DNA. Translation: BAB09532.1.
CP002688 Genomic DNA. Translation: AED91440.1.
CP002688 Genomic DNA. Translation: AED91441.1.
AK117126 mRNA. Translation: BAC41804.1.
AY086663 mRNA. Translation: AAM63720.1.
AB493744 mRNA. Translation: BAH30582.1.
PIRiT49924.
RefSeqiNP_196536.1. NM_121011.3. [Q9LXD7-1]
UniGeneiAt.32464.

Genome annotation databases

EnsemblPlantsiAT5G09740.1; AT5G09740.1; AT5G09740. [Q9LXD7-1]
GeneIDi830834.
KEGGiath:AT5G09740.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL353994 Genomic DNA. Translation: CAB89356.1.
AB020752 Genomic DNA. Translation: BAB09532.1.
CP002688 Genomic DNA. Translation: AED91440.1.
CP002688 Genomic DNA. Translation: AED91441.1.
AK117126 mRNA. Translation: BAC41804.1.
AY086663 mRNA. Translation: AAM63720.1.
AB493744 mRNA. Translation: BAH30582.1.
PIRiT49924.
RefSeqiNP_196536.1. NM_121011.3. [Q9LXD7-1]
UniGeneiAt.32464.

3D structure databases

ProteinModelPortaliQ9LXD7.
SMRiQ9LXD7. Positions 172-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16112. 1 interaction.

Proteomic databases

PRIDEiQ9LXD7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G09740.1; AT5G09740.1; AT5G09740. [Q9LXD7-1]
GeneIDi830834.
KEGGiath:AT5G09740.

Organism-specific databases

TAIRiAT5G09740.

Phylogenomic databases

eggNOGiCOG5027.
HOGENOMiHOG000182457.
InParanoidiQ9LXD7.
KOiK11308.
OMAiLDEWTQL.
PhylomeDBiQ9LXD7.

Enzyme and pathway databases

BioCyciARA:AT5G09740-MONOMER.
BRENDAi2.3.1.48. 399.

Miscellaneous databases

PROiQ9LXD7.

Gene expression databases

GenevestigatoriQ9LXD7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo/shadow_dom.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
IPR015880. Znf_C2H2-like.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
PROSITEiPS51726. MYST_HAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "ORF cloning and analysis of Arabidopsis transcription factor genes."
    Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y., Takagi M.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes."
    Pandey R., Mueller A., Napoli C.A., Selinger D.A., Pikaard C.S., Richards E.J., Bender J., Mount D.W., Jorgensen R.A.
    Nucleic Acids Res. 30:5036-5055(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, NOMENCLATURE.
  8. "Erasure of histone acetylation by Arabidopsis HDA6 mediates large-scale gene silencing in nucleolar dominance."
    Earley K., Lawrence R.J., Pontes O., Reuther R., Enciso A.J., Silva M., Neves N., Gross M., Viegas W., Pikaard C.S.
    Genes Dev. 20:1283-1293(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "In vitro specificities of Arabidopsis co-activator histone acetyltransferases: implications for histone hyperacetylation in gene activation."
    Earley K.W., Shook M.S., Brower-Toland B., Hicks L., Pikaard C.S.
    Plant J. 52:615-626(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "The MYST histone acetyltransferases are essential for gametophyte development in Arabidopsis."
    Latrasse D., Benhamed M., Henry Y., Domenichini S., Kim W., Zhou D.X., Delarue M.
    BMC Plant Biol. 8:121-121(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  11. "Participation of chromatin-remodeling proteins in the repair of ultraviolet-B-damaged DNA."
    Campi M., D'Andrea L., Emiliani J., Casati P.
    Plant Physiol. 158:981-995(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY UV.
  12. "Requirement of histone acetyltransferases HAM1 and HAM2 for epigenetic modification of FLC in regulating flowering in Arabidopsis."
    Xiao J., Zhang H., Xing L., Xu S., Liu H., Chong K., Xu Y.
    J. Plant Physiol. 170:444-451(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  13. "Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes."
    Xu Y., Gan E.S., Zhou J., Wee W.Y., Zhang X., Ito T.
    Nucleic Acids Res. 42:10960-10974(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MRG1 AND MRG2.
    Strain: cv. Columbia.

Entry informationi

Entry nameiMYST2_ARATH
AccessioniPrimary (citable) accession number: Q9LXD7
Secondary accession number(s): C0SVP1
, F4KE11, Q8GZ97, Q8LCD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: October 1, 2000
Last modified: April 29, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Knockdown expression of both HAM1 and HAM2 results in earlier flowering.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.