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Protein

Beta-D-xylosidase 3

Gene

BXL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of arabinan. Can hydrolyze (1,3)-alpha-, (1,2)-alpha-linked side group residues and non-reducing terminal L-arabinofuranose residues of debranched (1,5)-alpha-L-arabinan backbone. Acts also as a beta-D-xylosidase, releasing D-xylose from arabinoxylan and xylan.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=0.26 mM for p-nitrophenyl-beta-D-xylopyranoside (at 37 degrees Celsius)1 Publication
  2. KM=3.52 mM for p-nitrophenyl-alpha-L-arabinofuranoside (at 37 degrees Celsius)1 Publication
  3. KM=5.5 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 4.7.1 Publication

Temperature dependencei

Optimum temperature is 65 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei298 – 2981By similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: TAIR

GO - Biological processi

  1. arabinan catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G09730-MONOMER.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-D-xylosidase 3 (EC:3.2.1.-)
Short name:
AtBXL3
Alternative name(s):
Alpha-L-arabinofuranosidase (EC:3.2.1.55)
Gene namesi
Name:BXL3
Synonyms:XYL3
Ordered Locus Names:At5g09730
ORF Names:F17I14.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G09730.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Reduced seeds size and delayed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 773750Beta-D-xylosidase 3PRO_0000384058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi770 – 7701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9LXD6.
PRIDEiQ9LXD6.

Expressioni

Tissue specificityi

Expressed in flowers and siliques, in the early stage of seed formation and not at seed maturation. Detected exclusively in the endosperm of very young seeds when the embryo is at the globular stage.1 Publication

Gene expression databases

GenevestigatoriQ9LXD6.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G09730.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LXD6.
SMRiQ9LXD6. Positions 58-764.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 3 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000031216.
InParanoidiQ9LXD6.
KOiK15920.
OMAiANDFEDW.
PhylomeDBiQ9LXD6.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LXD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRNRALFS VSTLFLCFIV CISEQSNNQS SPVFACDVTG NPSLAGLRFC
60 70 80 90 100
NAGLSIKARV TDLVGRLTLE EKIGFLTSKA IGVSRLGIPS YKWWSEALHG
110 120 130 140 150
VSNVGGGSRF TGQVPGATSF PQVILTAASF NVSLFQAIGK VVSTEARAMY
160 170 180 190 200
NVGSAGLTFW SPNVNIFRDP RWGRGQETPG EDPTLSSKYA VAYVKGLQET
210 220 230 240 250
DGGDPNRLKV AACCKHYTAY DIDNWRNVNR LTFNAVVNQQ DLADTFQPPF
260 270 280 290 300
KSCVVDGHVA SVMCSYNQVN GKPTCADPDL LSGVIRGQWQ LNGYIVSDCD
310 320 330 340 350
SVDVLFRKQH YAKTPEEAVA KSLLAGLDLN CDHFNGQHAM GAVKAGLVNE
360 370 380 390 400
TAIDKAISNN FATLMRLGFF DGDPKKQLYG GLGPKDVCTA DNQELARDGA
410 420 430 440 450
RQGIVLLKNS AGSLPLSPSA IKTLAVIGPN ANATETMIGN YHGVPCKYTT
460 470 480 490 500
PLQGLAETVS STYQLGCNVA CVDADIGSAV DLAASADAVV LVVGADQSIE
510 520 530 540 550
REGHDRVDLY LPGKQQELVT RVAMAARGPV VLVIMSGGGF DITFAKNDKK
560 570 580 590 600
ITSIMWVGYP GEAGGLAIAD VIFGRHNPSG NLPMTWYPQS YVEKVPMSNM
610 620 630 640 650
NMRPDKSKGY PGRSYRFYTG ETVYAFADAL TYTKFDHQLI KAPRLVSLSL
660 670 680 690 700
DENHPCRSSE CQSLDAIGPH CENAVEGGSD FEVHLNVKNT GDRAGSHTVF
710 720 730 740 750
LFTTSPQVHG SPIKQLLGFE KIRLGKSEEA VVRFNVNVCK DLSVVDETGK
760 770
RKIALGHHLL HVGSLKHSLN ISV
Length:773
Mass (Da):83,222
Last modified:October 1, 2000 - v1
Checksum:i3C98ED6D3876621C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti488 – 4881A → S in BAD94522 (Ref. 6) Curated
Sequence conflicti569 – 5691A → P in BAD94522 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020752 Genomic DNA. Translation: BAB09531.1.
AL353994 Genomic DNA. Translation: CAB89357.1.
CP002688 Genomic DNA. Translation: AED91439.1.
AY053409 mRNA. Translation: AAK96639.1.
AK221979 mRNA. Translation: BAD94522.1.
PIRiT49925.
RefSeqiNP_196535.1. NM_121010.2.
UniGeneiAt.47498.

Genome annotation databases

EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730.
GeneIDi830833.
KEGGiath:AT5G09730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020752 Genomic DNA. Translation: BAB09531.1.
AL353994 Genomic DNA. Translation: CAB89357.1.
CP002688 Genomic DNA. Translation: AED91439.1.
AY053409 mRNA. Translation: AAK96639.1.
AK221979 mRNA. Translation: BAD94522.1.
PIRiT49925.
RefSeqiNP_196535.1. NM_121010.2.
UniGeneiAt.47498.

3D structure databases

ProteinModelPortaliQ9LXD6.
SMRiQ9LXD6. Positions 58-764.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G09730.1-P.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Proteomic databases

PaxDbiQ9LXD6.
PRIDEiQ9LXD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730.
GeneIDi830833.
KEGGiath:AT5G09730.

Organism-specific databases

TAIRiAT5G09730.

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000031216.
InParanoidiQ9LXD6.
KOiK15920.
OMAiANDFEDW.
PhylomeDBiQ9LXD6.

Enzyme and pathway databases

BioCyciARA:AT5G09730-MONOMER.

Gene expression databases

GenevestigatoriQ9LXD6.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis."
    Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L.
    J. Exp. Bot. 57:2339-2351(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-31, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 487-773.
    Strain: cv. Columbia.
  7. "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
    Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
    Plant J. 33:677-690(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiBXL3_ARATH
AccessioniPrimary (citable) accession number: Q9LXD6
Secondary accession number(s): Q56WQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.