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Q9LXD6

- BXL3_ARATH

UniProt

Q9LXD6 - BXL3_ARATH

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Protein
Beta-D-xylosidase 3
Gene
BXL3, XYL3, At5g09730, F17I14.80
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of arabinan. Can hydrolyze (1,3)-alpha-, (1,2)-alpha-linked side group residues and non-reducing terminal L-arabinofuranose residues of debranched (1,5)-alpha-L-arabinan backbone. Acts also as a beta-D-xylosidase, releasing D-xylose from arabinoxylan and xylan.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=0.26 mM for p-nitrophenyl-beta-D-xylopyranoside (at 37 degrees Celsius)1 Publication
  2. KM=3.52 mM for p-nitrophenyl-alpha-L-arabinofuranoside (at 37 degrees Celsius)
  3. KM=5.5 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)

pH dependencei

Optimum pH is 4.7.

Temperature dependencei

Optimum temperature is 65 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei298 – 2981 By similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. arabinan catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G09730-MONOMER.

Protein family/group databases

CAZyiGH3. Glycoside Hydrolase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-D-xylosidase 3 (EC:3.2.1.-)
Short name:
AtBXL3
Alternative name(s):
Alpha-L-arabinofuranosidase (EC:3.2.1.55)
Gene namesi
Name:BXL3
Synonyms:XYL3
Ordered Locus Names:At5g09730
ORF Names:F17I14.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G09730.

Subcellular locationi

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Reduced seeds size and delayed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 Publication
Add
BLAST
Chaini24 – 773750Beta-D-xylosidase 3
PRO_0000384058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi349 – 3491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi432 – 4321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi770 – 7701N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9LXD6.
PRIDEiQ9LXD6.

Expressioni

Tissue specificityi

Expressed in flowers and siliques, in the early stage of seed formation and not at seed maturation. Detected exclusively in the endosperm of very young seeds when the embryo is at the globular stage.1 Publication

Gene expression databases

GenevestigatoriQ9LXD6.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G09730.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LXD6.
SMRiQ9LXD6. Positions 58-764.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1472.
HOGENOMiHOG000031216.
InParanoidiQ9LXD6.
KOiK15920.
OMAiDAYCLAS.
PhylomeDBiQ9LXD6.

Family and domain databases

Gene3Di3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProiIPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR30620. PTHR30620. 1 hit.
PfamiPF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view]
PRINTSiPR00133. GLHYDRLASE3.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LXD6-1 [UniParc]FASTAAdd to Basket

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MASRNRALFS VSTLFLCFIV CISEQSNNQS SPVFACDVTG NPSLAGLRFC    50
NAGLSIKARV TDLVGRLTLE EKIGFLTSKA IGVSRLGIPS YKWWSEALHG 100
VSNVGGGSRF TGQVPGATSF PQVILTAASF NVSLFQAIGK VVSTEARAMY 150
NVGSAGLTFW SPNVNIFRDP RWGRGQETPG EDPTLSSKYA VAYVKGLQET 200
DGGDPNRLKV AACCKHYTAY DIDNWRNVNR LTFNAVVNQQ DLADTFQPPF 250
KSCVVDGHVA SVMCSYNQVN GKPTCADPDL LSGVIRGQWQ LNGYIVSDCD 300
SVDVLFRKQH YAKTPEEAVA KSLLAGLDLN CDHFNGQHAM GAVKAGLVNE 350
TAIDKAISNN FATLMRLGFF DGDPKKQLYG GLGPKDVCTA DNQELARDGA 400
RQGIVLLKNS AGSLPLSPSA IKTLAVIGPN ANATETMIGN YHGVPCKYTT 450
PLQGLAETVS STYQLGCNVA CVDADIGSAV DLAASADAVV LVVGADQSIE 500
REGHDRVDLY LPGKQQELVT RVAMAARGPV VLVIMSGGGF DITFAKNDKK 550
ITSIMWVGYP GEAGGLAIAD VIFGRHNPSG NLPMTWYPQS YVEKVPMSNM 600
NMRPDKSKGY PGRSYRFYTG ETVYAFADAL TYTKFDHQLI KAPRLVSLSL 650
DENHPCRSSE CQSLDAIGPH CENAVEGGSD FEVHLNVKNT GDRAGSHTVF 700
LFTTSPQVHG SPIKQLLGFE KIRLGKSEEA VVRFNVNVCK DLSVVDETGK 750
RKIALGHHLL HVGSLKHSLN ISV 773
Length:773
Mass (Da):83,222
Last modified:October 1, 2000 - v1
Checksum:i3C98ED6D3876621C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti488 – 4881A → S in BAD94522. 1 Publication
Sequence conflicti569 – 5691A → P in BAD94522. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB020752 Genomic DNA. Translation: BAB09531.1.
AL353994 Genomic DNA. Translation: CAB89357.1.
CP002688 Genomic DNA. Translation: AED91439.1.
AY053409 mRNA. Translation: AAK96639.1.
AK221979 mRNA. Translation: BAD94522.1.
PIRiT49925.
RefSeqiNP_196535.1. NM_121010.2.
UniGeneiAt.47498.

Genome annotation databases

EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730.
GeneIDi830833.
KEGGiath:AT5G09730.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB020752 Genomic DNA. Translation: BAB09531.1 .
AL353994 Genomic DNA. Translation: CAB89357.1 .
CP002688 Genomic DNA. Translation: AED91439.1 .
AY053409 mRNA. Translation: AAK96639.1 .
AK221979 mRNA. Translation: BAD94522.1 .
PIRi T49925.
RefSeqi NP_196535.1. NM_121010.2.
UniGenei At.47498.

3D structure databases

ProteinModelPortali Q9LXD6.
SMRi Q9LXD6. Positions 58-764.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT5G09730.1-P.

Protein family/group databases

CAZyi GH3. Glycoside Hydrolase Family 3.

Proteomic databases

PaxDbi Q9LXD6.
PRIDEi Q9LXD6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G09730.1 ; AT5G09730.1 ; AT5G09730 .
GeneIDi 830833.
KEGGi ath:AT5G09730.

Organism-specific databases

TAIRi AT5G09730.

Phylogenomic databases

eggNOGi COG1472.
HOGENOMi HOG000031216.
InParanoidi Q9LXD6.
KOi K15920.
OMAi DAYCLAS.
PhylomeDBi Q9LXD6.

Enzyme and pathway databases

BioCyci ARA:AT5G09730-MONOMER.

Gene expression databases

Genevestigatori Q9LXD6.

Family and domain databases

Gene3Di 3.20.20.300. 1 hit.
3.40.50.1700. 1 hit.
InterProi IPR026891. Fn3-like.
IPR026892. Glyco_hydro_3.
IPR002772. Glyco_hydro_3_C.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR30620. PTHR30620. 1 hit.
Pfami PF14310. Fn3-like. 1 hit.
PF00933. Glyco_hydro_3. 1 hit.
PF01915. Glyco_hydro_3_C. 1 hit.
[Graphical view ]
PRINTSi PR00133. GLHYDRLASE3.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52279. SSF52279. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis."
    Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L.
    J. Exp. Bot. 57:2339-2351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-31, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 487-773.
    Strain: cv. Columbia.
  7. "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
    Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
    Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiBXL3_ARATH
AccessioniPrimary (citable) accession number: Q9LXD6
Secondary accession number(s): Q56WQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi