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Protein

Beta-D-xylosidase 3

Gene

BXL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of arabinan. Can hydrolyze (1,3)-alpha-, (1,2)-alpha-linked side group residues and non-reducing terminal L-arabinofuranose residues of debranched (1,5)-alpha-L-arabinan backbone. Acts also as a beta-D-xylosidase, releasing D-xylose from arabinoxylan and xylan.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=0.26 mM for p-nitrophenyl-beta-D-xylopyranoside (at 37 degrees Celsius)1 Publication
  2. KM=3.52 mM for p-nitrophenyl-alpha-L-arabinofuranoside (at 37 degrees Celsius)1 Publication
  3. KM=5.5 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 4.7.1 Publication

    Temperature dependencei

    Optimum temperature is 65 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei298By similarity1

    GO - Molecular functioni

    • alpha-L-arabinofuranosidase activity Source: TAIR
    • xylan 1,4-beta-xylosidase activity Source: GO_Central

    GO - Biological processi

    • arabinan catabolic process Source: TAIR
    • xylan catabolic process Source: GO_Central
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G09730-MONOMER.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 3 (EC:3.2.1.-)
    Short name:
    AtBXL3
    Alternative name(s):
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Gene namesi
    Name:BXL3
    Synonyms:XYL3
    Ordered Locus Names:At5g09730
    ORF Names:F17I14.80
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G09730.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Reduced seeds size and delayed germination.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 231 PublicationAdd BLAST23
    ChainiPRO_000038405824 – 773Beta-D-xylosidase 3Add BLAST750

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi131N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi349N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi432N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi770N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9LXD6.
    PRIDEiQ9LXD6.

    Expressioni

    Tissue specificityi

    Expressed in flowers and siliques, in the early stage of seed formation and not at seed maturation. Detected exclusively in the endosperm of very young seeds when the embryo is at the globular stage.1 Publication

    Gene expression databases

    GenevisibleiQ9LXD6. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G09730.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXD6.
    SMRiQ9LXD6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IE0I. Eukaryota.
    COG1472. LUCA.
    HOGENOMiHOG000031216.
    InParanoidiQ9LXD6.
    KOiK15920.
    OMAiHCENAVE.
    OrthoDBiEOG0936046Q.
    PhylomeDBiQ9LXD6.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SMARTiSM01217. Fn3_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LXD6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASRNRALFS VSTLFLCFIV CISEQSNNQS SPVFACDVTG NPSLAGLRFC
    60 70 80 90 100
    NAGLSIKARV TDLVGRLTLE EKIGFLTSKA IGVSRLGIPS YKWWSEALHG
    110 120 130 140 150
    VSNVGGGSRF TGQVPGATSF PQVILTAASF NVSLFQAIGK VVSTEARAMY
    160 170 180 190 200
    NVGSAGLTFW SPNVNIFRDP RWGRGQETPG EDPTLSSKYA VAYVKGLQET
    210 220 230 240 250
    DGGDPNRLKV AACCKHYTAY DIDNWRNVNR LTFNAVVNQQ DLADTFQPPF
    260 270 280 290 300
    KSCVVDGHVA SVMCSYNQVN GKPTCADPDL LSGVIRGQWQ LNGYIVSDCD
    310 320 330 340 350
    SVDVLFRKQH YAKTPEEAVA KSLLAGLDLN CDHFNGQHAM GAVKAGLVNE
    360 370 380 390 400
    TAIDKAISNN FATLMRLGFF DGDPKKQLYG GLGPKDVCTA DNQELARDGA
    410 420 430 440 450
    RQGIVLLKNS AGSLPLSPSA IKTLAVIGPN ANATETMIGN YHGVPCKYTT
    460 470 480 490 500
    PLQGLAETVS STYQLGCNVA CVDADIGSAV DLAASADAVV LVVGADQSIE
    510 520 530 540 550
    REGHDRVDLY LPGKQQELVT RVAMAARGPV VLVIMSGGGF DITFAKNDKK
    560 570 580 590 600
    ITSIMWVGYP GEAGGLAIAD VIFGRHNPSG NLPMTWYPQS YVEKVPMSNM
    610 620 630 640 650
    NMRPDKSKGY PGRSYRFYTG ETVYAFADAL TYTKFDHQLI KAPRLVSLSL
    660 670 680 690 700
    DENHPCRSSE CQSLDAIGPH CENAVEGGSD FEVHLNVKNT GDRAGSHTVF
    710 720 730 740 750
    LFTTSPQVHG SPIKQLLGFE KIRLGKSEEA VVRFNVNVCK DLSVVDETGK
    760 770
    RKIALGHHLL HVGSLKHSLN ISV
    Length:773
    Mass (Da):83,222
    Last modified:October 1, 2000 - v1
    Checksum:i3C98ED6D3876621C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti488A → S in BAD94522 (Ref. 6) Curated1
    Sequence conflicti569A → P in BAD94522 (Ref. 6) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB020752 Genomic DNA. Translation: BAB09531.1.
    AL353994 Genomic DNA. Translation: CAB89357.1.
    CP002688 Genomic DNA. Translation: AED91439.1.
    AY053409 mRNA. Translation: AAK96639.1.
    AK221979 mRNA. Translation: BAD94522.1.
    PIRiT49925.
    RefSeqiNP_196535.1. NM_121010.3.
    UniGeneiAt.47498.

    Genome annotation databases

    EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730.
    GeneIDi830833.
    GrameneiAT5G09730.1; AT5G09730.1; AT5G09730.
    KEGGiath:AT5G09730.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB020752 Genomic DNA. Translation: BAB09531.1.
    AL353994 Genomic DNA. Translation: CAB89357.1.
    CP002688 Genomic DNA. Translation: AED91439.1.
    AY053409 mRNA. Translation: AAK96639.1.
    AK221979 mRNA. Translation: BAD94522.1.
    PIRiT49925.
    RefSeqiNP_196535.1. NM_121010.3.
    UniGeneiAt.47498.

    3D structure databases

    ProteinModelPortaliQ9LXD6.
    SMRiQ9LXD6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G09730.1.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Proteomic databases

    PaxDbiQ9LXD6.
    PRIDEiQ9LXD6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730.
    GeneIDi830833.
    GrameneiAT5G09730.1; AT5G09730.1; AT5G09730.
    KEGGiath:AT5G09730.

    Organism-specific databases

    TAIRiAT5G09730.

    Phylogenomic databases

    eggNOGiENOG410IE0I. Eukaryota.
    COG1472. LUCA.
    HOGENOMiHOG000031216.
    InParanoidiQ9LXD6.
    KOiK15920.
    OMAiHCENAVE.
    OrthoDBiEOG0936046Q.
    PhylomeDBiQ9LXD6.

    Enzyme and pathway databases

    BioCyciARA:AT5G09730-MONOMER.

    Miscellaneous databases

    PROiQ9LXD6.

    Gene expression databases

    GenevisibleiQ9LXD6. AT.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SMARTiSM01217. Fn3_like. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBXL3_ARATH
    AccessioniPrimary (citable) accession number: Q9LXD6
    Secondary accession number(s): Q56WQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: October 1, 2000
    Last modified: November 30, 2016
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.