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Q9LXD6

- BXL3_ARATH

UniProt

Q9LXD6 - BXL3_ARATH

Protein

Beta-D-xylosidase 3

Gene

BXL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Involved in the hydrolysis of arabinan. Can hydrolyze (1,3)-alpha-, (1,2)-alpha-linked side group residues and non-reducing terminal L-arabinofuranose residues of debranched (1,5)-alpha-L-arabinan backbone. Acts also as a beta-D-xylosidase, releasing D-xylose from arabinoxylan and xylan.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

    Kineticsi

    1. KM=0.26 mM for p-nitrophenyl-beta-D-xylopyranoside (at 37 degrees Celsius)1 Publication
    2. KM=3.52 mM for p-nitrophenyl-alpha-L-arabinofuranoside (at 37 degrees Celsius)1 Publication
    3. KM=5.5 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 4.7.1 Publication

    Temperature dependencei

    Optimum temperature is 65 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei298 – 2981By similarity

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: TAIR

    GO - Biological processi

    1. arabinan catabolic process Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G09730-MONOMER.

    Protein family/group databases

    CAZyiGH3. Glycoside Hydrolase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 3 (EC:3.2.1.-)
    Short name:
    AtBXL3
    Alternative name(s):
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Gene namesi
    Name:BXL3
    Synonyms:XYL3
    Ordered Locus Names:At5g09730
    ORF Names:F17I14.80
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G09730.

    Subcellular locationi

    GO - Cellular componenti

    1. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Reduced seeds size and delayed germination.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 773750Beta-D-xylosidase 3PRO_0000384058Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi349 – 3491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi770 – 7701N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9LXD6.
    PRIDEiQ9LXD6.

    Expressioni

    Tissue specificityi

    Expressed in flowers and siliques, in the early stage of seed formation and not at seed maturation. Detected exclusively in the endosperm of very young seeds when the embryo is at the globular stage.1 Publication

    Gene expression databases

    GenevestigatoriQ9LXD6.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G09730.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXD6.
    SMRiQ9LXD6. Positions 58-764.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1472.
    HOGENOMiHOG000031216.
    InParanoidiQ9LXD6.
    KOiK15920.
    OMAiDAYCLAS.
    PhylomeDBiQ9LXD6.

    Family and domain databases

    Gene3Di3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProiIPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR30620. PTHR30620. 1 hit.
    PfamiPF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view]
    PRINTSiPR00133. GLHYDRLASE3.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LXD6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASRNRALFS VSTLFLCFIV CISEQSNNQS SPVFACDVTG NPSLAGLRFC    50
    NAGLSIKARV TDLVGRLTLE EKIGFLTSKA IGVSRLGIPS YKWWSEALHG 100
    VSNVGGGSRF TGQVPGATSF PQVILTAASF NVSLFQAIGK VVSTEARAMY 150
    NVGSAGLTFW SPNVNIFRDP RWGRGQETPG EDPTLSSKYA VAYVKGLQET 200
    DGGDPNRLKV AACCKHYTAY DIDNWRNVNR LTFNAVVNQQ DLADTFQPPF 250
    KSCVVDGHVA SVMCSYNQVN GKPTCADPDL LSGVIRGQWQ LNGYIVSDCD 300
    SVDVLFRKQH YAKTPEEAVA KSLLAGLDLN CDHFNGQHAM GAVKAGLVNE 350
    TAIDKAISNN FATLMRLGFF DGDPKKQLYG GLGPKDVCTA DNQELARDGA 400
    RQGIVLLKNS AGSLPLSPSA IKTLAVIGPN ANATETMIGN YHGVPCKYTT 450
    PLQGLAETVS STYQLGCNVA CVDADIGSAV DLAASADAVV LVVGADQSIE 500
    REGHDRVDLY LPGKQQELVT RVAMAARGPV VLVIMSGGGF DITFAKNDKK 550
    ITSIMWVGYP GEAGGLAIAD VIFGRHNPSG NLPMTWYPQS YVEKVPMSNM 600
    NMRPDKSKGY PGRSYRFYTG ETVYAFADAL TYTKFDHQLI KAPRLVSLSL 650
    DENHPCRSSE CQSLDAIGPH CENAVEGGSD FEVHLNVKNT GDRAGSHTVF 700
    LFTTSPQVHG SPIKQLLGFE KIRLGKSEEA VVRFNVNVCK DLSVVDETGK 750
    RKIALGHHLL HVGSLKHSLN ISV 773
    Length:773
    Mass (Da):83,222
    Last modified:October 1, 2000 - v1
    Checksum:i3C98ED6D3876621C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti488 – 4881A → S in BAD94522. 1 PublicationCurated
    Sequence conflicti569 – 5691A → P in BAD94522. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB020752 Genomic DNA. Translation: BAB09531.1.
    AL353994 Genomic DNA. Translation: CAB89357.1.
    CP002688 Genomic DNA. Translation: AED91439.1.
    AY053409 mRNA. Translation: AAK96639.1.
    AK221979 mRNA. Translation: BAD94522.1.
    PIRiT49925.
    RefSeqiNP_196535.1. NM_121010.2.
    UniGeneiAt.47498.

    Genome annotation databases

    EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730.
    GeneIDi830833.
    KEGGiath:AT5G09730.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB020752 Genomic DNA. Translation: BAB09531.1 .
    AL353994 Genomic DNA. Translation: CAB89357.1 .
    CP002688 Genomic DNA. Translation: AED91439.1 .
    AY053409 mRNA. Translation: AAK96639.1 .
    AK221979 mRNA. Translation: BAD94522.1 .
    PIRi T49925.
    RefSeqi NP_196535.1. NM_121010.2.
    UniGenei At.47498.

    3D structure databases

    ProteinModelPortali Q9LXD6.
    SMRi Q9LXD6. Positions 58-764.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G09730.1-P.

    Protein family/group databases

    CAZyi GH3. Glycoside Hydrolase Family 3.

    Proteomic databases

    PaxDbi Q9LXD6.
    PRIDEi Q9LXD6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G09730.1 ; AT5G09730.1 ; AT5G09730 .
    GeneIDi 830833.
    KEGGi ath:AT5G09730.

    Organism-specific databases

    TAIRi AT5G09730.

    Phylogenomic databases

    eggNOGi COG1472.
    HOGENOMi HOG000031216.
    InParanoidi Q9LXD6.
    KOi K15920.
    OMAi DAYCLAS.
    PhylomeDBi Q9LXD6.

    Enzyme and pathway databases

    BioCyci ARA:AT5G09730-MONOMER.

    Gene expression databases

    Genevestigatori Q9LXD6.

    Family and domain databases

    Gene3Di 3.20.20.300. 1 hit.
    3.40.50.1700. 1 hit.
    InterProi IPR026891. Fn3-like.
    IPR026892. Glyco_hydro_3.
    IPR002772. Glyco_hydro_3_C.
    IPR001764. Glyco_hydro_3_N.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR30620. PTHR30620. 1 hit.
    Pfami PF14310. Fn3-like. 1 hit.
    PF00933. Glyco_hydro_3. 1 hit.
    PF01915. Glyco_hydro_3_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00133. GLHYDRLASE3.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52279. SSF52279. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
      DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Purification, functional characterization, cloning, and identification of mutants of a seed-specific arabinan hydrolase in Arabidopsis."
      Minic Z., Do C.-T., Rihouey C., Morin H., Lerouge P., Jouanin L.
      J. Exp. Bot. 57:2339-2351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-31, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 487-773.
      Strain: cv. Columbia.
    7. "AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta-xylosidase gene, is involved in secondary cell wall metabolism and plant development."
      Goujon T., Minic Z., El Amrani A., Lerouxel O., Aletti E., Lapierre C., Joseleau J.-P., Jouanin L.
      Plant J. 33:677-690(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiBXL3_ARATH
    AccessioniPrimary (citable) accession number: Q9LXD6
    Secondary accession number(s): Q56WQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3