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Protein

Beta-D-xylosidase 3

Gene

BXL3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of arabinan. Can hydrolyze (1,3)-alpha-, (1,2)-alpha-linked side group residues and non-reducing terminal L-arabinofuranose residues of debranched (1,5)-alpha-L-arabinan backbone. Acts also as a beta-D-xylosidase, releasing D-xylose from arabinoxylan and xylan.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

Kineticsi

  1. KM=0.26 mM for p-nitrophenyl-beta-D-xylopyranoside (at 37 degrees Celsius)1 Publication
  2. KM=3.52 mM for p-nitrophenyl-alpha-L-arabinofuranoside (at 37 degrees Celsius)1 Publication
  3. KM=5.5 mM for (1,5)-alpha-L-arabinobiose (at 37 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 4.7.1 Publication

    Temperature dependencei

    Optimum temperature is 65 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei298By similarity1

    GO - Molecular functioni

    • alpha-L-arabinofuranosidase activity Source: TAIR

    GO - Biological processi

    • arabinan catabolic process Source: TAIR

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT5G09730-MONOMER

    Protein family/group databases

    CAZyiGH3 Glycoside Hydrolase Family 3

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-D-xylosidase 3 (EC:3.2.1.-)
    Short name:
    AtBXL3
    Alternative name(s):
    Alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Gene namesi
    Name:BXL3
    Synonyms:XYL3
    Ordered Locus Names:At5g09730
    ORF Names:F17I14.80
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G09730
    TAIRilocus:2144756 AT5G09730

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Reduced seeds size and delayed germination.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 231 PublicationAdd BLAST23
    ChainiPRO_000038405824 – 773Beta-D-xylosidase 3Add BLAST750

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi432N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi770N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9LXD6
    PRIDEiQ9LXD6

    Expressioni

    Tissue specificityi

    Expressed in flowers and siliques, in the early stage of seed formation and not at seed maturation. Detected exclusively in the endosperm of very young seeds when the embryo is at the globular stage.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9LXD6 baseline and differential
    GenevisibleiQ9LXD6 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G09730.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LXD6
    SMRiQ9LXD6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 3 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IE0I Eukaryota
    COG1472 LUCA
    HOGENOMiHOG000031216
    InParanoidiQ9LXD6
    KOiK15920
    OMAiGNLPMTW
    OrthoDBiEOG0936046Q
    PhylomeDBiQ9LXD6

    Family and domain databases

    Gene3Di2.60.40.10, 1 hit
    3.20.20.300, 1 hit
    3.40.50.1700, 1 hit
    InterProiView protein in InterPro
    IPR026891 Fn3-like
    IPR002772 Glyco_hydro_3_C
    IPR036881 Glyco_hydro_3_C_sf
    IPR001764 Glyco_hydro_3_N
    IPR036962 Glyco_hydro_3_N_sf
    IPR017853 Glycoside_hydrolase_SF
    IPR013783 Ig-like_fold
    PfamiView protein in Pfam
    PF14310 Fn3-like, 1 hit
    PF00933 Glyco_hydro_3, 1 hit
    PF01915 Glyco_hydro_3_C, 1 hit
    PRINTSiPR00133 GLHYDRLASE3
    SMARTiView protein in SMART
    SM01217 Fn3_like, 1 hit
    SUPFAMiSSF51445 SSF51445, 1 hit
    SSF52279 SSF52279, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LXD6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASRNRALFS VSTLFLCFIV CISEQSNNQS SPVFACDVTG NPSLAGLRFC
    60 70 80 90 100
    NAGLSIKARV TDLVGRLTLE EKIGFLTSKA IGVSRLGIPS YKWWSEALHG
    110 120 130 140 150
    VSNVGGGSRF TGQVPGATSF PQVILTAASF NVSLFQAIGK VVSTEARAMY
    160 170 180 190 200
    NVGSAGLTFW SPNVNIFRDP RWGRGQETPG EDPTLSSKYA VAYVKGLQET
    210 220 230 240 250
    DGGDPNRLKV AACCKHYTAY DIDNWRNVNR LTFNAVVNQQ DLADTFQPPF
    260 270 280 290 300
    KSCVVDGHVA SVMCSYNQVN GKPTCADPDL LSGVIRGQWQ LNGYIVSDCD
    310 320 330 340 350
    SVDVLFRKQH YAKTPEEAVA KSLLAGLDLN CDHFNGQHAM GAVKAGLVNE
    360 370 380 390 400
    TAIDKAISNN FATLMRLGFF DGDPKKQLYG GLGPKDVCTA DNQELARDGA
    410 420 430 440 450
    RQGIVLLKNS AGSLPLSPSA IKTLAVIGPN ANATETMIGN YHGVPCKYTT
    460 470 480 490 500
    PLQGLAETVS STYQLGCNVA CVDADIGSAV DLAASADAVV LVVGADQSIE
    510 520 530 540 550
    REGHDRVDLY LPGKQQELVT RVAMAARGPV VLVIMSGGGF DITFAKNDKK
    560 570 580 590 600
    ITSIMWVGYP GEAGGLAIAD VIFGRHNPSG NLPMTWYPQS YVEKVPMSNM
    610 620 630 640 650
    NMRPDKSKGY PGRSYRFYTG ETVYAFADAL TYTKFDHQLI KAPRLVSLSL
    660 670 680 690 700
    DENHPCRSSE CQSLDAIGPH CENAVEGGSD FEVHLNVKNT GDRAGSHTVF
    710 720 730 740 750
    LFTTSPQVHG SPIKQLLGFE KIRLGKSEEA VVRFNVNVCK DLSVVDETGK
    760 770
    RKIALGHHLL HVGSLKHSLN ISV
    Length:773
    Mass (Da):83,222
    Last modified:October 1, 2000 - v1
    Checksum:i3C98ED6D3876621C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti488A → S in BAD94522 (Ref. 6) Curated1
    Sequence conflicti569A → P in BAD94522 (Ref. 6) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB020752 Genomic DNA Translation: BAB09531.1
    AL353994 Genomic DNA Translation: CAB89357.1
    CP002688 Genomic DNA Translation: AED91439.1
    AY053409 mRNA Translation: AAK96639.1
    AK221979 mRNA Translation: BAD94522.1
    PIRiT49925
    RefSeqiNP_196535.1, NM_121010.3
    UniGeneiAt.47498

    Genome annotation databases

    EnsemblPlantsiAT5G09730.1; AT5G09730.1; AT5G09730
    GeneIDi830833
    GrameneiAT5G09730.1; AT5G09730.1; AT5G09730
    KEGGiath:AT5G09730

    Similar proteinsi

    Entry informationi

    Entry nameiBXL3_ARATH
    AccessioniPrimary (citable) accession number: Q9LXD6
    Secondary accession number(s): Q56WQ6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 96 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

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