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Protein

eIF-2-alpha kinase GCN2

Gene

GCN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 eIF-2-alpha in response to low amino acid availability. Plays a role as an activator of the general amino acid control pathway required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha either to a competitive inhibitor of translation initiation, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, and hence allowing reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Enzyme regulationi

The kinase activity is stimulated upon binding to uncharged tRNAs.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei454 – 4541ATPPROSITE-ProRule annotation
Active sitei586 – 5861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi431 – 4399ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Protein biosynthesis, Stress response, Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciARA:AT3G59410-MONOMER.
ARA:GQT-1223-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
eIF-2-alpha kinase GCN2By similarity
Alternative name(s):
serine/threonine-protein kinase GCN2By similarity (EC:2.7.11.1)
Gene namesi
Name:GCN2By similarity
Ordered Locus Names:At3g59410
ORF Names:F25L23_270
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G59410.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12411241eIF-2-alpha kinase GCN2PRO_0000085961Add
BLAST

Proteomic databases

PaxDbiQ9LX30.
PRIDEiQ9LX30.

PTM databases

iPTMnetiQ9LX30.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, buds, flowers, siliques and seedlings.1 Publication

Gene expression databases

ExpressionAtlasiQ9LX30. baseline and differential.
GenevisibleiQ9LX30. AT.

Interactioni

Subunit structurei

Homodimer; homodimerization is important for kinase activation by uncharged tRNAs.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G59410.2.

Structurei

3D structure databases

ProteinModelPortaliQ9LX30.
SMRiQ9LX30. Positions 38-147, 418-747, 789-1224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 148112RWDPROSITE-ProRule annotationAdd
BLAST
Domaini425 – 731307Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni819 – 1219401Histidyl-tRNA synthetase-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 115Poly-Lys

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RWD domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1035. Eukaryota.
COG0124. LUCA.
HOGENOMiHOG000083904.
InParanoidiQ9LX30.
PhylomeDBiQ9LX30.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR004154. Anticodon-bd.
IPR024435. HisRS-related_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR006575. RWD-domain.
IPR008266. Tyr_kinase_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF12745. HGTP_anticodon2. 1 hit.
PF00069. Pkinase. 1 hit.
PF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF54495. SSF54495. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50908. RWD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LX30-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRSSSKKKK KRGGSGRRGQ LKDHGSNADE DNELLSEEIT ALSAIFQEDC
60 70 80 90 100
KVVSDSRSPP QIAIKLRPYS KDMGYEDTDI SAMLIVRCLP GYPYKCPKLQ
110 120 130 140 150
ITPEQGLTTA DAEKLLSLLE DQANSNAREG RVMIFNLVEA AQEFLSEIIP
160 170 180 190 200
ESHDEESVPC LTAHRSTQFI EQPMLSNIAK SCSGGPFVYG FIDLFSGLED
210 220 230 240 250
ARNWSLTPDE NRGIVSSVQS HPLDTSRILH QKPDKNLKRF EDHAKEEVAL
260 270 280 290 300
PAPIAKLNTV QEENVDDTSI SSFDSSKSTD DVESGLFQNE KKESNLQDDT
310 320 330 340 350
AEDDSTNSES ESLGSWSSDS LAQDQVPQIS KKDLLMVHLL RVACTSRGPL
360 370 380 390 400
ADALPQITDE LHELGILSEE VLDLASKSSP DFNRTFEHAF NQNMASTSVP
410 420 430 440 450
QFWEPPSDSC EPNASLPSSR YLNDFEELKP LGQGGFGHVV LCKNKLDGRQ
460 470 480 490 500
YAVKKIRLKD KEIPVNSRIV REVATLSRLQ HQHVVRYYQA WFETGVVDPF
510 520 530 540 550
AGANWGSKTA GSSMFSYSGA VSTEIPEQDN NLESTYLYIQ MEYCPRTLRQ
560 570 580 590 600
VFESYNHFDK DFAWHLIRQI VEGLAHIHGQ GIIHRDFTPN NIFFDARNDI
610 620 630 640 650
KIGDFGLAKF LKLEQLDQDG GFSTDVAGSG VDSTGQAGTY FYTAPEIEQD
660 670 680 690 700
WPKIDEKADM YSLGVVFFEL WHPFGTAMER HVILTNLKLK GELPLKWVNE
710 720 730 740 750
FPEQASLLRR LMSPSPSDRP SATELLKHAF PPRMESELLD NILRIMQTSE
760 770 780 790 800
DSSVYDRVVS VIFDEEVLEM KSHQSSRSRL CADDSYIQYT EINTELRDYV
810 820 830 840 850
VEITKEVFRQ HCAKHLEVIP MRLLSDCPQF SRKTVKLLTN GGDMLELCYE
860 870 880 890 900
LRLPFVHWIS VNQKSSFKRY EISHVYRRAI GHSPPNPCLQ ADFDIVGGTL
910 920 930 940 950
SLTEAEVLKV IVDITTHIFH RGSCDIHLNH GDLLDAIWSW AGIKAEHRRK
960 970 980 990 1000
VAELLSMMGS LRPQSSERKL KWVFIRRQLL QELKLPEAVV NRLQTVASRF
1010 1020 1030 1040 1050
CGDADQALPR LRGALRADRP TRKALDELSN LLTYLRVWRI EEHVHIDVLM
1060 1070 1080 1090 1100
PPTESYHRNL FFQVFLTKEN SSGTSNDGVL LAVGGRYDWL VQEVCDREHK
1110 1120 1130 1140 1150
MNLPGAVGVS LALETIFQHL PMDLRPIRNE VSTSVLVCSR GGGGLLVQRM
1160 1170 1180 1190 1200
ELVAELWEKS IKAEFVPTPD PSLTEQYEYA NEHEIKCLVI ITESGVAQNQ
1210 1220 1230 1240
IEFVKVRHLE LKKEKVVGRE ELVKFLLDAM AVQFRNPSVW S
Length:1,241
Mass (Da):140,322
Last modified:June 21, 2005 - v2
Checksum:iD098A1B7B01E1E0D
GO

Sequence cautioni

The sequence CAB91611.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ459823 mRNA. Translation: CAD30860.1.
AL356014 Genomic DNA. Translation: CAB91611.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79918.1.
PIRiT49009.
RefSeqiNP_191500.2. NM_115803.2. [Q9LX30-1]
UniGeneiAt.34633.

Genome annotation databases

EnsemblPlantsiAT3G59410.1; AT3G59410.1; AT3G59410. [Q9LX30-1]
GeneIDi825110.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ459823 mRNA. Translation: CAD30860.1.
AL356014 Genomic DNA. Translation: CAB91611.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79918.1.
PIRiT49009.
RefSeqiNP_191500.2. NM_115803.2. [Q9LX30-1]
UniGeneiAt.34633.

3D structure databases

ProteinModelPortaliQ9LX30.
SMRiQ9LX30. Positions 38-147, 418-747, 789-1224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G59410.2.

PTM databases

iPTMnetiQ9LX30.

Proteomic databases

PaxDbiQ9LX30.
PRIDEiQ9LX30.

Protocols and materials databases

DNASUi825110.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G59410.1; AT3G59410.1; AT3G59410. [Q9LX30-1]
GeneIDi825110.

Organism-specific databases

TAIRiAT3G59410.

Phylogenomic databases

eggNOGiKOG1035. Eukaryota.
COG0124. LUCA.
HOGENOMiHOG000083904.
InParanoidiQ9LX30.
PhylomeDBiQ9LX30.

Enzyme and pathway databases

BioCyciARA:AT3G59410-MONOMER.
ARA:GQT-1223-MONOMER.

Miscellaneous databases

PROiQ9LX30.

Gene expression databases

ExpressionAtlasiQ9LX30. baseline and differential.
GenevisibleiQ9LX30. AT.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR004154. Anticodon-bd.
IPR024435. HisRS-related_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR006575. RWD-domain.
IPR008266. Tyr_kinase_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF12745. HGTP_anticodon2. 1 hit.
PF00069. Pkinase. 1 hit.
PF05773. RWD. 1 hit.
[Graphical view]
SMARTiSM00591. RWD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF54495. SSF54495. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50908. RWD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of an Arabidopsis homologue of GCN2, a protein kinase involved in co-ordinated response to amino acid starvation."
    Zhang Y., Dickinson R., Paul M.J., Halford N.G.
    Planta 217:668-675(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiGCN2_ARATH
AccessioniPrimary (citable) accession number: Q9LX30
Secondary accession number(s): Q8H2D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: April 13, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Although it is unknown whether it is a serine/threonine or a tyrosine protein kinase, it is strongly related to the serine/threonine-protein kinase family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.