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Protein

Serine/threonine-protein kinase-like protein ACR4

Gene

ACR4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls formative cell division in meristems, including root tips and lateral root initiation zones of the pericycle, in response to CLE40 signal. Acts with CLE40p peptide as a ligand-receptor pair in a signal transduction pathway, coordinating movement of the root tip and organization of cell divisions in the root meristem. Required during embryogenesis and development, probably for the differentiation of protoderm and epidermal cells. Involved in the regulation of cellular organization during the development of sepal margins and ovule integument outgrowth. Can phosphorylate ALE2.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei540ATPCurated1
Active sitei641Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi518 – 526ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB-KW
  • transmembrane receptor protein kinase activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • flower morphogenesis Source: EnsemblPlants
  • lateral root formation Source: TAIR
  • plant epidermal cell differentiation Source: EnsemblPlants
  • protein autophosphorylation Source: EnsemblPlants
  • regulation of asymmetric cell division Source: TAIR
  • root cap development Source: TAIR
  • root development Source: TAIR

Keywordsi

Molecular functionDevelopmental protein, Kinase, Receptor, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase-like protein ACR4 (EC:2.7.11.1)
Alternative name(s):
Protein CRINKLY 4
Short name:
AtCR4
Gene namesi
Name:ACR4
Ordered Locus Names:At3g59420
ORF Names:F25L23.280
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT3G59420
TAIRilocus:2081227 AT3G59420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 434ExtracellularSequence analysisAdd BLAST405
Transmembranei435 – 455HelicalSequence analysisAdd BLAST21
Topological domaini456 – 895CytoplasmicSequence analysisAdd BLAST440

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced fertility due to abnormal embryogenesis and integument formation. Abnormal seed coat and leaves epidermis, with transient fusion between adjacent developing leaves and reduced hydrophobicity of leaf surfaces.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi221C → Y in acr4-7; abnormal embryogenesis, and reduced internalization into vesicles. 1 Publication1
Mutagenesisi433A → F: Enhanced dimerization. 1 Publication1
Mutagenesisi438G → V: Enhanced dimerization. 1 Publication1
Mutagenesisi452A → C: Unchanged dimerization. 1 Publication1
Mutagenesisi540K → L, M or W: Loss of kinase activity. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000038275030 – 895Serine/threonine-protein kinase-like protein ACR4Add BLAST866

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi158N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi290N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi347 ↔ 370PROSITE-ProRule annotation
Disulfide bondi373 ↔ 387PROSITE-ProRule annotation
Disulfide bondi377 ↔ 395PROSITE-ProRule annotation
Glycosylationi398N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi410N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei475Phosphoserine1

Post-translational modificationi

Autophosphorylated and phosphorylated by ALE2.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LX29
PRIDEiQ9LX29

Expressioni

Tissue specificityi

Expressed in seedlings, floral buds, siliques, leaves, shoot apical meristems (SAM), and, to a lower extent, in roots.2 Publications

Developmental stagei

First observed in all young embryo cells. At the globular stage, restricted to apical region. Later confined to the protoderm area leading to cotyledon primordia and the root apex. In mature embryos, mostly localized in the L1 layer of the SAM, apical regions of cotyledons and the root apex, and, to a lower extent, in protoderm of other regions. In seedlings, expressed in developing tissues of the shoot, including the SAM and epidermis of organs primordia, especially in L1 layer cells. In roots, localized in quiescent center (QC) central cells, columella initials and cells below the QC, the lateral root cap (LRC) and the initial cells destined to give rise to the root epidermal cell file and the LRC. Expressed in epidermal emerged from under the LRC, with levels vanishing in elongation zone. Specifically detected in the small daughter cells after the first asymmetric pericycle cell division during lateral roots emergence. Subsequently, the expression expands to the adjacent small daughter cells from the second asymmetric cell division, resulting in a central core-specific expression pattern.4 Publications

Inductioni

By CLE40 in root quiescent center (QC).1 Publication

Gene expression databases

ExpressionAtlasiQ9LX29 baseline and differential
GenevisibleiQ9LX29 AT

Interactioni

Subunit structurei

Homodimer (Probable). Interacts with PP2A3 (PubMed:26792519).Curated1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi10426, 2 interactors
STRINGi3702.AT3G59420.1

Structurei

3D structure databases

ProteinModelPortaliQ9LX29
SMRiQ9LX29
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 731Add BLAST36
Repeati77 – 1122Add BLAST36
Repeati130 – 1673Add BLAST38
Repeati169 – 2024Add BLAST34
Repeati210 – 2455Add BLAST36
Repeati262 – 2966Add BLAST35
Repeati301 – 3397Add BLAST39
Repeati346 – 395TNFR-CysAdd BLAST50
Domaini512 – 789Protein kinasePROSITE-ProRule annotationAdd BLAST278

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 3397 X 36 AA repeatsAdd BLAST302

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF4A Eukaryota
COG0515 LUCA
HOGENOMiHOG000029541
InParanoidiQ9LX29
OMAiYNMTRNF
OrthoDBiEOG093601OK
PhylomeDBiQ9LX29

Family and domain databases

Gene3Di2.130.10.30, 2 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR009091 RCC1/BLIP-II
IPR008271 Ser/Thr_kinase_AS
IPR001368 TNFR/NGFR_Cys_rich_reg
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SM00208 TNFR, 1 hit
SUPFAMiSSF50985 SSF50985, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50050 TNFR_NGFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LX29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMFETRARE WILLVKLVLF TSIWQLASAL GSMSSIAISY GEGGSVFCGL
60 70 80 90 100
KSDGSHLVVC YGSNSAILYG TPGHLQFIGL TGGDGFMCGL LMLSHQPYCW
110 120 130 140 150
GNSAFIQMGV PQPMTKGAEY LEVSAGDYHL CGLRKPIVGR RKNSNIISSS
160 170 180 190 200
LVDCWGYNMT RNFVFDKQLH SLSAGSEFNC ALSSKDKSVF CWGDENSSQV
210 220 230 240 250
ISLIPKEKKF QKIAAGGYHV CGILDGLESR VLCWGKSLEF EEEVTGTSTE
260 270 280 290 300
EKILDLPPKE PLLAVVGGKF YACGIKRYDH SAVCWGFFVN RSTPAPTGIG
310 320 330 340 350
FYDLAAGNYF TCGVLTGTSM SPVCWGLGFP ASIPLAVSPG LCIDTPCPPG
360 370 380 390 400
THELSNQENS PCKFTGSHIC LPCSTSCPPG MYQKSVCTER SDQVCVYNCS
410 420 430 440 450
SCSSHDCSSN CSSSATSGGK EKGKFWSLQL PIATAEIGFA LFLVAVVSIT
460 470 480 490 500
AALYIRYRLR NCRCSENDTR SSKDSAFTKD NGKIRPDLDE LQKRRRARVF
510 520 530 540 550
TYEELEKAAD GFKEESIVGK GSFSCVYKGV LRDGTTVAVK RAIMSSDKQK
560 570 580 590 600
NSNEFRTELD LLSRLNHAHL LSLLGYCEEC GERLLVYEFM AHGSLHNHLH
610 620 630 640 650
GKNKALKEQL DWVKRVTIAV QAARGIEYLH GYACPPVIHR DIKSSNILID
660 670 680 690 700
EEHNARVADF GLSLLGPVDS GSPLAELPAG TLGYLDPEYY RLHYLTTKSD
710 720 730 740 750
VYSFGVLLLE ILSGRKAIDM HYEEGNIVEW AVPLIKAGDI NALLDPVLKH
760 770 780 790 800
PSEIEALKRI VSVACKCVRM RGKDRPSMDK VTTALERALA QLMGNPSSEQ
810 820 830 840 850
PILPTEVVLG SSRMHKKSWR IGSKRSGSEN TEFRGGSWIT FPSVTSSQRR
860 870 880 890
KSSASEGDVA EEEDEGRKQQ EALRSLEEEI GPASPGQSLF LHHNF
Length:895
Mass (Da):98,181
Last modified:October 1, 2000 - v1
Checksum:i1B6BD5BB5F0B9DFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB074762 mRNA Translation: BAB91132.1
AL356014 Genomic DNA Translation: CAB91612.1
CP002686 Genomic DNA Translation: AEE79920.1
PIRiT49010
RefSeqiNP_191501.1, NM_115804.4
UniGeneiAt.28062

Genome annotation databases

EnsemblPlantsiAT3G59420.1; AT3G59420.1; AT3G59420
GeneIDi825111
GrameneiAT3G59420.1; AT3G59420.1; AT3G59420
KEGGiath:AT3G59420

Entry informationi

Entry nameiACR4L_ARATH
AccessioniPrimary (citable) accession number: Q9LX29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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