Q9LWM4 (CIPK5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CBL-interacting protein kinase 5
EC=2.7.11.1

Alternative name(s):
OsCIPK05

Gene names

Name:	CIPK5
Ordered Locus Names:	Os01g0206700, LOC_Os01g10890
ORF Names:	OSJNBa0016I09.5, P0451C06.36, OsJ_000797

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Manganese By similarity.
Induction	By drought stress and abscisic acid (ABA). Ref.7
Domain	The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 NAF domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Manganese Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	CBL-interacting protein kinase 5		PRO_0000338363
Regions
	Domain	12 – 266	255	Protein kinase
	Domain	300 – 334	35	NAF
	Nucleotide binding	18 – 26	9	ATP By similarity
	Region	152 – 181	30	Activation loop By similarity
	Region	340 – 369	30	PPI By similarity
Sites
	Active site	134	1	Proton acceptor By similarity
	Binding site	41	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LWM4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 613833E798BAE649	FASTA	461	51,966
	10 20 30 40 50 60 MEKKASILMN RYELGRMLGQ GTFAKVYHAR NLASNQSVAI KVIDKEKVLR VGMIDQIKRE 70 80 90 100 110 120 ISIMRLVRHP NIVQLHEVMA SKSKIYFAME YVRGGELFSR VARGRLKEDA ARKYFQQLIG 130 140 150 160 170 180 AVDFCHSRGV YHRDLKPENL LVDENGNLKV SDFGLSAFKE CQKQDGLLHT TCGTPAYVAP 190 200 210 220 230 240 EIINKRGYDG AKADIWSCGV ILFVLLAGYL PFHDSNLMEM YRKISKGDVK FPQWFTTDVR 250 260 270 280 290 300 RLLSRLLDPN PNIRITVEKL VEHPWFKKGY KPAVMLSQPN ESNNLKDVHT AFSADHKDNE 310 320 330 340 350 360 GKAKEPASSL KPVSLNAFDI ISLSKGFDLS GLFENDKEQK ADSRFMTQKP ASAIVSKLEQ 370 380 390 400 410 420 IAETESFKVK KQDGLVKLQG SKEGRKGQLA IDAEIFEVTP SFFVVEVKKS AGDTLEYEKF 430 440 450 460 CNKGLRPSLR DICWDGQSEH PSLAQSSTLT QSSKSISRHA I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks." Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J. Plant Physiol. 134:43-58(2004) [PubMed: 14730064] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement." Xiang Y., Huang Y., Xiong L. Plant Physiol. 144:1416-1428(2007) [PubMed: 17535819] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP001551 Genomic DNA. Translation: BAA92972.1. AP003052 Genomic DNA. Translation: BAB92151.1. AP008207 Genomic DNA. Translation: BAF04259.1. CM000138 Genomic DNA. Translation: EAZ10972.1. AK065589 mRNA. No translation available.
RefSeq	NP_001042345.1. NM_001048880.1.
UniGene	Os.1411.
3D structure databases
HSSP	HSSP built from PDB template 2FH9 based on UniProtKB P06782.
ProteinModelPortal	Q9LWM4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LWM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4327093.
KEGG	osa:4327093.
NMPDR	fig|39947.1.peg.5305.
Organism-specific databases
Gramene	Q9LWM4.
Phylogenomic databases
HOGENOM	HBG755340.
OMA	IRITVEK.
PhylomeDB	Q9LWM4.
ProtClustDB	CLSN2691189.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR018451. NAF/FISL_domain. IPR004041. NAF_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
Pfam	PF03822. NAF. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50816. NAF. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CIPK5_ORYSJ

Accession

Primary (citable) accession number: Q9LWM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	October 1, 2000
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program