Q9LWB5 (RK23_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L23, chloroplastic

Alternative name(s):
PRPL23

Gene names

Name:

RPL23

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Chenopodioideae › Anserineae › Spinacia

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to 23S rRNA By similarity. Located at the polypeptide exit tunnel on the outside of the subunit.
Subunit structure	Part of the 50S ribosomal subunit. Ref.2
Subcellular location	Plastid › chloroplast.
Miscellaneous	This protein is the 80S cytosolic ortholog and is imported into the plastid, unlike the case in most plastids where it is the bacterial ortholog and is encoded in the plastid. Two forms of the protein exist; they have the same N-terminal sequence and approximately the same molecular weight.
Sequence similarities	Belongs to the ribosomal protein L23P family.
Mass spectrometry	Molecular mass is 13553.5 Da from positions 77 - 198. Determined by ESI. Ref.3

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Chloroplast Ref.2 Ref.3
	Chain	77 – 198	122	50S ribosomal protein L23, chloroplastic		PRO_5000146571
Experimental info
	Sequence conflict	83	1	P → L AA sequence Ref.2
	Sequence conflict	93	1	P → N AA sequence Ref.2
	Sequence conflict	101	1	K → I AA sequence Ref.2
	Sequence conflict	108	1	I → V AA sequence Ref.2
	Sequence conflict	171	1	T → TE AA sequence Ref.2
	Sequence conflict	178	1	L → I AA sequence Ref.2
	Sequence conflict	189 – 193	5	KKIGI → TEASK AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9LWB5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 624DF1D4032BB70F	FASTA	198	21,800
	10 20 30 40 50 60 MATTAPNLHS LSSSFAFSNP SSNVSATSFT FQIPNKKAQI SCISSKKLHT QKSFNFHDAV 70 80 90 100 110 120 TPMNKPSFGR DLMVAQATEA VAPTTEEAAT SQPKTSKKAK KLKYPRRILD VYQILQSPII 130 140 150 160 170 180 TEAAIKNIAD ENSLLFTVDV RADKKMIREA ISNFFGVKVR KVNTLIRPDG TKKAYIMLNK 190 EYNASELAKK IGIFPGGN

References

[1]	"Nucleotide sequence of the cDNA coding for chloroplast ribosomal protein L23 of spinach." Jayabaskaran C., Subramanian A.R. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Matador.
[2]	"Protein substitution in chloroplast ribosome evolution. A eukaryotic cytosolic protein has replaced its organelle homologue (L23) in spinach." Bubunenko M.G., Schmidt J., Subramanian A.R. J. Mol. Biol. 240:28-41(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 77-113; 122-136 AND 156-193, IDENTIFICATION AS A SUBUNIT OF THE CHLOROPLAST RIBOSOME, DEMONSTRATION OF TWO FORMS. Strain: cv. Alwaro. Tissue: Leaf.
[3]	"The plastid ribosomal proteins. Identification of all the proteins in the 50S subunit of an organelle ribosome (chloroplast)." Yamaguchi K., Subramanian A.R. J. Biol. Chem. 275:28466-28482(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 77-82, MASS SPECTROMETRY. Strain: cv. Alwaro. Tissue: Leaf.
[4]	"Cryo-EM study of the spinach chloroplast ribosome reveals the structural and functional roles of plastid-specific ribosomal proteins." Sharma M.R., Wilson D.N., Datta P.P., Barat C., Schluenzen F., Fucini P., Agrawal R.K. Proc. Natl. Acad. Sci. U.S.A. 104:19315-19320(2007) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (9.4 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X90414 mRNA. Translation: CAA62040.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BBO	electron microscopy	9.40	V	1-198	[»]

ProteinModelPortal

Q9LWB5.

SMR

Q9LWB5. Positions 110-194.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.70.330. 1 hit.

InterPro

IPR012677. Nucleotide-bd_a/b_plait.
IPR012678. Ribosomal_L23/L15e_core_dom.
IPR013025. Ribosomal_L25/23.
[Graphical view]

Pfam

PF00276. Ribosomal_L23. 1 hit.
[Graphical view]

SUPFAM

SSF54189. L23_L15e_core. 1 hit.

PROSITE

PS00050. RIBOSOMAL_L23. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9LWB5.

Entry information

Entry name

RK23_SPIOL

Accession

Primary (citable) accession number: Q9LWB5
Secondary accession number(s): Q9T2N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program