Q9LW44 (MANP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative mannan endo-1,4-beta-mannosidase P
EC=3.2.1.78

Alternative name(s):
Beta-mannanase P
Endo-beta-1,4-mannanase P
Short name=AtMANP

Gene names

Name:	MANP
Ordered Locus Names:	At3g30540
ORF Names:	MQP15.18

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence BAB01021.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB01021.1 differs from that shown. Reason: Erroneous termination at position 85. Translated as Trp. The sequence BAB01021.1 differs from that shown. Reason: Frameshift at position 350.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 408	385	Putative mannan endo-1,4-beta-mannosidase P		PRO_0000277481
Sites
	Active site	202	1	Proton donor By similarity
	Active site	322	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LW44 [UniParc]. Last modified February 8, 2011. Version 3. Checksum: 5536B88BF62A373A	FASTA	408	45,356
	10 20 30 40 50 60 MKCLCFIVLL AIVIAQSYVG VEAAPSDGFV SRNGVQFILN GKPFYANGFN AYWLAYEATD 70 80 90 100 110 120 PATRFKITNV FQNATSLGLT IARTWGFRNG AIYRALQTAP GSYDEQTFQG LDFGIAEAKR 130 140 150 160 170 180 VGIKLIIPLV NNWDDYGGKK QYVDWARSKG EMVSSNDDFY RNPVIKEFYK NHVKTMLNRV 190 200 210 220 230 240 NTFTKVAYKD EPASMAWQLM NEPRCGVDRS GKTLMAWINE MALFVKSVDP NHLLSTGHEG 250 260 270 280 290 300 FYGDSSPERK NSLNPVSANT VGADFIANHN IDAIDFASMH CGSDLWFQRL DQNSRLAFIK 310 320 330 340 350 360 RWLEGHIEDA QNNLKKPVIL AEFGLGSDTP RYTLANRDDV FTTTYDIIYI STQKGGSAAG 370 380 390 400 ALFWEVISEG VSNFAGPSSI ILSDKSSTVN IISEQRRKMG LLGGTKGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed: 16897088] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016878 Genomic DNA. Translation: BAB01021.1. Sequence problems.
IPI	IPI00536485.
3D structure databases
ProteinModelPortal	Q9LW44.
SMR	Q9LW44. Positions 26-400.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT3G30540 in contig BA000014_GR.
Organism-specific databases
TAIR	At3g30540.
Phylogenomic databases
eggNOG	euNOG11227.
HOGENOM	HBG316339.
InParanoid	Q9LW44.
PhylomeDB	Q9LW44.
Gene expression databases
Genevestigator	Q9LW44.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANP_ARATH

Accession

Primary (citable) accession number: Q9LW44

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 8, 2011
Last modified:	December 14, 2011
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program