Q9LW44 (MANP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative mannan endo-1,4-beta-mannosidase P
EC=3.2.1.78

Alternative name(s):
Beta-mannanase P
Endo-beta-1,4-mannanase P
Short name=AtMANP

Gene names

Name:	MANP
Ordered Locus Names:	At3g30540
ORF Names:	MQP15.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Uncertain

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AEE77650.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AEE77650.1 differs from that shown. Reason: Erroneous termination at position 85. Translated as Trp. The sequence AEE77650.1 differs from that shown. Reason: Frameshift at position 350. The sequence BAB01021.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB01021.1 differs from that shown. Reason: Erroneous termination at position 85. Translated as Trp. The sequence BAB01021.1 differs from that shown. Reason: Frameshift at position 350.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 408	385	Putative mannan endo-1,4-beta-mannosidase P		PRO_0000277481
Sites
	Active site	202	1	Proton donor By similarity
	Active site	322	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	73	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LW44 [UniParc]. Last modified February 8, 2011. Version 3. Checksum: 5536B88BF62A373A	FASTA	408	45,356
	10 20 30 40 50 60 MKCLCFIVLL AIVIAQSYVG VEAAPSDGFV SRNGVQFILN GKPFYANGFN AYWLAYEATD 70 80 90 100 110 120 PATRFKITNV FQNATSLGLT IARTWGFRNG AIYRALQTAP GSYDEQTFQG LDFGIAEAKR 130 140 150 160 170 180 VGIKLIIPLV NNWDDYGGKK QYVDWARSKG EMVSSNDDFY RNPVIKEFYK NHVKTMLNRV 190 200 210 220 230 240 NTFTKVAYKD EPASMAWQLM NEPRCGVDRS GKTLMAWINE MALFVKSVDP NHLLSTGHEG 250 260 270 280 290 300 FYGDSSPERK NSLNPVSANT VGADFIANHN IDAIDFASMH CGSDLWFQRL DQNSRLAFIK 310 320 330 340 350 360 RWLEGHIEDA QNNLKKPVIL AEFGLGSDTP RYTLANRDDV FTTTYDIIYI STQKGGSAAG 370 380 390 400 ALFWEVISEG VSNFAGPSSI ILSDKSSTVN IISEQRRKMG LLGGTKGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016878 Genomic DNA. Translation: BAB01021.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE77650.1. Sequence problems.
IPI	IPI00536485. IPI01019894.
RefSeq	NP_189675.2. NM_113955.2.
UniGene	At.53601.
3D structure databases
ProteinModelPortal	Q9LW44.
SMR	Q9LW44. Positions 27-402.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G30540.1-P.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	822768.
KEGG	ath:AT3G30540.
Organism-specific databases
TAIR	At3g30540.
Phylogenomic databases
eggNOG	COG3934.
InParanoid	Q9LW44.
PhylomeDB	Q9LW44.
Gene expression databases
ArrayExpress	Q9LW44.
Genevestigator	Q9LW44.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MANP_ARATH

Accession

Primary (citable) accession number: Q9LW44
Secondary accession number(s): F4J6N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program