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Q9LW44 (MANP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative mannan endo-1,4-beta-mannosidase P

EC=3.2.1.78
Alternative name(s):
Beta-mannanase P
Endo-beta-1,4-mannanase P
Short name=AtMANP
Gene names
Name:MANP
Ordered Locus Names:At3g30540
ORF Names:MQP15.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceUncertain

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Caution

Could be the product of a pseudogene.

Sequence caution

The sequence AEE77650.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AEE77650.1 differs from that shown. Reason: Erroneous termination at position 85. Translated as Trp.

The sequence AEE77650.1 differs from that shown. Reason: Frameshift at position 350.

The sequence BAB01021.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAB01021.1 differs from that shown. Reason: Erroneous termination at position 85. Translated as Trp.

The sequence BAB01021.1 differs from that shown. Reason: Frameshift at position 350.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 408385Putative mannan endo-1,4-beta-mannosidase P
PRO_0000277481

Sites

Active site2021Proton donor By similarity
Active site3221Nucleophile By similarity

Amino acid modifications

Glycosylation731N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9LW44 [UniParc].

Last modified February 8, 2011. Version 3.
Checksum: 5536B88BF62A373A

FASTA40845,356
        10         20         30         40         50         60 
MKCLCFIVLL AIVIAQSYVG VEAAPSDGFV SRNGVQFILN GKPFYANGFN AYWLAYEATD 

        70         80         90        100        110        120 
PATRFKITNV FQNATSLGLT IARTWGFRNG AIYRALQTAP GSYDEQTFQG LDFGIAEAKR 

       130        140        150        160        170        180 
VGIKLIIPLV NNWDDYGGKK QYVDWARSKG EMVSSNDDFY RNPVIKEFYK NHVKTMLNRV 

       190        200        210        220        230        240 
NTFTKVAYKD EPASMAWQLM NEPRCGVDRS GKTLMAWINE MALFVKSVDP NHLLSTGHEG 

       250        260        270        280        290        300 
FYGDSSPERK NSLNPVSANT VGADFIANHN IDAIDFASMH CGSDLWFQRL DQNSRLAFIK 

       310        320        330        340        350        360 
RWLEGHIEDA QNNLKKPVIL AEFGLGSDTP RYTLANRDDV FTTTYDIIYI STQKGGSAAG 

       370        380        390        400 
ALFWEVISEG VSNFAGPSSI ILSDKSSTVN IISEQRRKMG LLGGTKGK 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016878 Genomic DNA. Translation: BAB01021.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77650.1. Sequence problems.
RefSeqNP_189675.2. NM_113955.2.
UniGeneAt.53601.

3D structure databases

ProteinModelPortalQ9LW44.
SMRQ9LW44. Positions 27-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G30540.1-P.

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID822768.
KEGGath:AT3G30540.

Organism-specific databases

TAIRAT3G30540.

Phylogenomic databases

eggNOGCOG3934.
InParanoidQ9LW44.
PhylomeDBQ9LW44.

Enzyme and pathway databases

BioCycARA:AT3G30540-MONOMER.

Gene expression databases

GenevestigatorQ9LW44.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMANP_ARATH
AccessionPrimary (citable) accession number: Q9LW44
Secondary accession number(s): F4J6N6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 8, 2011
Last modified: April 16, 2014
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names