Reviewed, UniProtKB/Swiss-Prot Q9LW29 (AFB2_ARATH)

Names and origin

Protein names

Recommended name:
    Protein AUXIN SIGNALING F-BOX 2

Gene names

Name:	AFB2
Synonyms:	LRF2
Ordered Locus Names:	At3g26810
ORF Names:	MDJ14.12

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Confers sensitivity to the virulent bacterial pathogen P.syringae By similarity. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin.
Subunit structure	Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity. Interacts with Aux/IAA proteins (IAA7) in an auxin-dependent manner.
Subcellular location	Nucleus. Ref.4
Tissue specificity	Ubiquitous, with higer levels in seedlings. Ref.4
Induction	Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22). Ref.6
Domain	The F-box is necessary for the interaction with SKP1 By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Auxin signaling pathway Plant defense
Cellular component	Nucleus
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB defense response Inferred from electronic annotation. Source: UniProtKB-KW pollen maturation Inferred from genetic interaction. Source: TAIR response to molecule of bacterial origin Ref.6 Inferred from expression pattern. Source: TAIR stamen development Inferred from genetic interaction. Source: TAIR
Cellular component	SCF ubiquitin ligase complex Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	auxin binding Ref.5 Inferred from sequence or structural similarity. Source: UniProtKB inositol hexakisphosphate binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IAA7	Q38825	1	EBI-617556,EBI-602959

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Protein AUXIN SIGNALING F-BOX 2		PRO_0000272243
Regions
	Domain	1 – 47	47	F-box
	Region	76 – 77	2	Interaction with auxin-responsive proteins By similarity
	Region	108 – 109	2	Myo-inositol hexakisphosphate binding By similarity
	Region	343 – 348	6	Interaction with auxin-responsive proteins By similarity
	Region	396 – 398	3	Myo-inositol hexakisphosphate binding By similarity
	Region	400 – 404	5	Interaction with auxin-responsive proteins By similarity
	Region	459 – 460	2	Interaction with auxin-responsive proteins By similarity
	Region	479 – 480	2	Myo-inositol hexakisphosphate binding By similarity
Sites
	Binding site	69	1	Myo-inositol hexakisphosphate By similarity
	Binding site	340	1	Myo-inositol hexakisphosphate By similarity
	Binding site	431	1	Myo-inositol hexakisphosphate By similarity
	Binding site	504	1	Myo-inositol hexakisphosphate By similarity
	Site	134	1	Interaction with auxin-responsive proteins By similarity
	Site	160	1	Interaction with auxin-responsive proteins By similarity
	Site	375	1	Interaction with auxin-responsive proteins By similarity
	Site	484	1	Interaction with auxin-responsive proteins By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LW29-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D383C4E0A76D50D6	FASTA	575	64,605
	10 20 30 40 50 60 MNYFPDEVIE HVFDFVTSHK DRNAISLVCK SWYKIERYSR QKVFIGNCYA INPERLLRRF 70 80 90 100 110 120 PCLKSLTLKG KPHFADFNLV PHEWGGFVLP WIEALARSRV GLEELRLKRM VVTDESLELL 130 140 150 160 170 180 SRSFVNFKSL VLVSCEGFTT DGLASIAANC RHLRDLDLQE NEIDDHRGQW LSCFPDTCTT 190 200 210 220 230 240 LVTLNFACLE GETNLVALER LVARSPNLKS LKLNRAVPLD ALARLMACAP QIVDLGVGSY 250 260 270 280 290 300 ENDPDSESYL KLMAVIKKCT SLRSLSGFLE AAPHCLSAFH PICHNLTSLN LSYAAEIHGS 310 320 330 340 350 360 HLIKLIQHCK KLQRLWILDS IGDKGLEVVA STCKELQELR VFPSDLLGGG NTAVTEEGLV 370 380 390 400 410 420 AISAGCPKLH SILYFCQQMT NAALVTVAKN CPNFIRFRLC ILEPNKPDHV TSQPLDEGFG 430 440 450 460 470 480 AIVKACKSLR RLSLSGLLTD QVFLYIGMYA NQLEMLSIAF AGDTDKGMLY VLNGCKKMKK 490 500 510 520 530 540 LEIRDSPFGD TALLADVSKY ETMRSLWMSS CEVTLSGCKR LAEKAPWLNV EIINENDNNR 550 560 570 MEENGHEGRQ KVDKLYLYRT VVGTRMDAPP FVWIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression." Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H. Plant Mol. Biol. 49:69-79(2002) [PubMed: 12008900] [Abstract] Cited for: LEUCINE-RICH REPEATS.
[4]	"Plant development is regulated by a family of auxin receptor F box proteins." Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M. Dev. Cell 9:109-119(2005) [PubMed: 15992545] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH IAA7.
[5]	"The F-box protein TIR1 is an auxin receptor." Dharmasiri N., Dharmasiri S., Estelle M. Nature 435:441-445(2005) [PubMed: 15917797] [Abstract] Cited for: FUNCTION.
[6]	"A plant miRNA contributes to antibacterial resistance by repressing auxin signaling." Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G. Science 312:436-439(2006) [PubMed: 16627744] [Abstract] Cited for: FUNCTION, INDUCTION.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB016889 Genomic DNA. Translation: BAB01228.1. AY062568 mRNA. Translation: AAL32646.1. BT010396 mRNA. Translation: AAQ56839.1.
IPI	IPI00520683.
RefSeq	NP_566800.1.
UniGene	At.6031
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LW29. 1 interaction.
Proteomic databases
PRIDE	Q9LW29.
Genome annotation databases
GeneID	822296.
GenomeReviews	Gene locus AT3G26810 in contig BA000014_GR.
KEGG	ath:AT3G26810.
NMPDR	fig|3702.1.peg.14963.
Organism-specific databases
TAIR	At3g26810.
Phylogenomic databases
OMA	Q9LW29. WLSCFPD.
Family and domain databases
InterPro	IPR001810. F-box. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFB2_ARATH

Accession

Primary (citable) accession number: Q9LW29

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)