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Q9LW29 (AFB2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein AUXIN SIGNALING F-BOX 2
Gene names
Name:AFB2
Synonyms:LRF2
Ordered Locus Names:At3g26810
ORF Names:MDJ14.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Confers sensitivity to the virulent bacterial pathogen P.syringae By similarity. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin. Ref.5 Ref.6 Ref.7

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity. Interacts with Aux/IAA proteins (IAA7) in an auxin-dependent manner. Ref.5

Subcellular location

Nucleus Ref.5.

Tissue specificity

Ubiquitous, with higer levels in seedlings. Ref.5

Induction

Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22). Ref.7

Domain

The F-box is necessary for the interaction with SKP1 By similarity.

Sequence similarities

Contains 1 F-box domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575Protein AUXIN SIGNALING F-BOX 2
PRO_0000272243

Regions

Domain1 – 4747F-box
Region76 – 772Interaction with auxin-responsive proteins By similarity
Region108 – 1092Myo-inositol hexakisphosphate binding By similarity
Region343 – 3486Interaction with auxin-responsive proteins By similarity
Region396 – 3983Myo-inositol hexakisphosphate binding By similarity
Region400 – 4045Interaction with auxin-responsive proteins By similarity
Region459 – 4602Interaction with auxin-responsive proteins By similarity
Region479 – 4802Myo-inositol hexakisphosphate binding By similarity

Sites

Binding site691Myo-inositol hexakisphosphate By similarity
Binding site3401Myo-inositol hexakisphosphate By similarity
Binding site4311Myo-inositol hexakisphosphate By similarity
Binding site5041Myo-inositol hexakisphosphate By similarity
Site1341Interaction with auxin-responsive proteins By similarity
Site1601Interaction with auxin-responsive proteins By similarity
Site3751Interaction with auxin-responsive proteins By similarity
Site4841Interaction with auxin-responsive proteins By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LW29 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D383C4E0A76D50D6

FASTA57564,605
        10         20         30         40         50         60 
MNYFPDEVIE HVFDFVTSHK DRNAISLVCK SWYKIERYSR QKVFIGNCYA INPERLLRRF 

        70         80         90        100        110        120 
PCLKSLTLKG KPHFADFNLV PHEWGGFVLP WIEALARSRV GLEELRLKRM VVTDESLELL 

       130        140        150        160        170        180 
SRSFVNFKSL VLVSCEGFTT DGLASIAANC RHLRDLDLQE NEIDDHRGQW LSCFPDTCTT 

       190        200        210        220        230        240 
LVTLNFACLE GETNLVALER LVARSPNLKS LKLNRAVPLD ALARLMACAP QIVDLGVGSY 

       250        260        270        280        290        300 
ENDPDSESYL KLMAVIKKCT SLRSLSGFLE AAPHCLSAFH PICHNLTSLN LSYAAEIHGS 

       310        320        330        340        350        360 
HLIKLIQHCK KLQRLWILDS IGDKGLEVVA STCKELQELR VFPSDLLGGG NTAVTEEGLV 

       370        380        390        400        410        420 
AISAGCPKLH SILYFCQQMT NAALVTVAKN CPNFIRFRLC ILEPNKPDHV TSQPLDEGFG 

       430        440        450        460        470        480 
AIVKACKSLR RLSLSGLLTD QVFLYIGMYA NQLEMLSIAF AGDTDKGMLY VLNGCKKMKK 

       490        500        510        520        530        540 
LEIRDSPFGD TALLADVSKY ETMRSLWMSS CEVTLSGCKR LAEKAPWLNV EIINENDNNR 

       550        560        570 
MEENGHEGRQ KVDKLYLYRT VVGTRMDAPP FVWIL 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression."
Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.
Plant Mol. Biol. 49:69-79(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: LEUCINE-RICH REPEATS.
[5]"Plant development is regulated by a family of auxin receptor F box proteins."
Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH IAA7.
[6]"The F-box protein TIR1 is an auxin receptor."
Dharmasiri N., Dharmasiri S., Estelle M.
Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016889 Genomic DNA. Translation: BAB01228.1.
CP002686 Genomic DNA. Translation: AEE77218.1.
AY062568 mRNA. Translation: AAL32646.1.
BT010396 mRNA. Translation: AAQ56839.1.
RefSeqNP_566800.1. NM_113593.2.
UniGeneAt.6031.

3D structure databases

ProteinModelPortalQ9LW29.
SMRQ9LW29. Positions 5-573.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid7626. 13 interactions.
DIPDIP-34608N.
IntActQ9LW29. 2 interactions.

Proteomic databases

PaxDbQ9LW29.
PRIDEQ9LW29.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G26810.1; AT3G26810.1; AT3G26810.
GeneID822296.
KEGGath:AT3G26810.

Organism-specific databases

TAIRAT3G26810.

Phylogenomic databases

eggNOGNOG259467.
HOGENOMHOG000239805.
InParanoidQ9LW29.
OMAAIVRECK.
PhylomeDBQ9LW29.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9LW29.

Family and domain databases

InterProIPR001810. F-box_dom.
[Graphical view]
PfamPF00646. F-box. 1 hit.
[Graphical view]
SMARTSM00256. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAFB2_ARATH
AccessionPrimary (citable) accession number: Q9LW29
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names