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Protein

Protein AUXIN SIGNALING F-BOX 2

Gene

AFB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Confers sensitivity to the virulent bacterial pathogen P.syringae (By similarity). Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin.By similarity3 Publications

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691Myo-inositol hexakisphosphateBy similarity
Sitei134 – 1341Interaction with auxin-responsive proteinsBy similarity
Sitei160 – 1601Interaction with auxin-responsive proteinsBy similarity
Binding sitei340 – 3401Myo-inositol hexakisphosphateBy similarity
Sitei375 – 3751Interaction with auxin-responsive proteinsBy similarity
Binding sitei431 – 4311Myo-inositol hexakisphosphateBy similarity
Sitei484 – 4841Interaction with auxin-responsive proteinsBy similarity
Binding sitei504 – 5041Myo-inositol hexakisphosphateBy similarity

GO - Molecular functioni

GO - Biological processi

  • auxin-activated signaling pathway Source: UniProtKB
  • defense response Source: UniProtKB-KW
  • pollen maturation Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • response to molecule of bacterial origin Source: TAIR
  • stamen development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Auxin signaling pathway, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein AUXIN SIGNALING F-BOX 2
Gene namesi
Name:AFB2
Synonyms:LRF2
Ordered Locus Names:At3g26810
ORF Names:MDJ14.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G26810.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB-SubCell
  • SCF ubiquitin ligase complex Source: UniProtKB
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Protein AUXIN SIGNALING F-BOX 2PRO_0000272243Add
BLAST

Proteomic databases

PaxDbiQ9LW29.
PRIDEiQ9LW29.

Expressioni

Tissue specificityi

Ubiquitous, with higer levels in seedlings.1 Publication

Inductioni

Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).1 Publication

Interactioni

Subunit structurei

Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Interacts with Aux/IAA proteins (IAA7) in an auxin-dependent manner.By similarity1 Publication

Protein-protein interaction databases

BioGridi7626. 13 interactions.
DIPiDIP-34608N.
IntActiQ9LW29. 2 interactions.
STRINGi3702.AT3G26810.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LW29.
SMRiQ9LW29. Positions 5-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 4747F-boxAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 772Interaction with auxin-responsive proteinsBy similarity
Regioni108 – 1092Myo-inositol hexakisphosphate bindingBy similarity
Regioni343 – 3486Interaction with auxin-responsive proteinsBy similarity
Regioni396 – 3983Myo-inositol hexakisphosphate bindingBy similarity
Regioni400 – 4045Interaction with auxin-responsive proteinsBy similarity
Regioni459 – 4602Interaction with auxin-responsive proteinsBy similarity
Regioni479 – 4802Myo-inositol hexakisphosphate bindingBy similarity

Domaini

The F-box is necessary for the interaction with SKP1.By similarity

Sequence similaritiesi

Contains 1 F-box domain.Curated

Phylogenomic databases

eggNOGiNOG259467.
HOGENOMiHOG000239805.
InParanoidiQ9LW29.
OMAiARLMACA.
PhylomeDBiQ9LW29.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LW29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYFPDEVIE HVFDFVTSHK DRNAISLVCK SWYKIERYSR QKVFIGNCYA
60 70 80 90 100
INPERLLRRF PCLKSLTLKG KPHFADFNLV PHEWGGFVLP WIEALARSRV
110 120 130 140 150
GLEELRLKRM VVTDESLELL SRSFVNFKSL VLVSCEGFTT DGLASIAANC
160 170 180 190 200
RHLRDLDLQE NEIDDHRGQW LSCFPDTCTT LVTLNFACLE GETNLVALER
210 220 230 240 250
LVARSPNLKS LKLNRAVPLD ALARLMACAP QIVDLGVGSY ENDPDSESYL
260 270 280 290 300
KLMAVIKKCT SLRSLSGFLE AAPHCLSAFH PICHNLTSLN LSYAAEIHGS
310 320 330 340 350
HLIKLIQHCK KLQRLWILDS IGDKGLEVVA STCKELQELR VFPSDLLGGG
360 370 380 390 400
NTAVTEEGLV AISAGCPKLH SILYFCQQMT NAALVTVAKN CPNFIRFRLC
410 420 430 440 450
ILEPNKPDHV TSQPLDEGFG AIVKACKSLR RLSLSGLLTD QVFLYIGMYA
460 470 480 490 500
NQLEMLSIAF AGDTDKGMLY VLNGCKKMKK LEIRDSPFGD TALLADVSKY
510 520 530 540 550
ETMRSLWMSS CEVTLSGCKR LAEKAPWLNV EIINENDNNR MEENGHEGRQ
560 570
KVDKLYLYRT VVGTRMDAPP FVWIL
Length:575
Mass (Da):64,605
Last modified:October 1, 2000 - v1
Checksum:iD383C4E0A76D50D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016889 Genomic DNA. Translation: BAB01228.1.
CP002686 Genomic DNA. Translation: AEE77218.1.
AY062568 mRNA. Translation: AAL32646.1.
BT010396 mRNA. Translation: AAQ56839.1.
RefSeqiNP_566800.1. NM_113593.2.
UniGeneiAt.6031.

Genome annotation databases

EnsemblPlantsiAT3G26810.1; AT3G26810.1; AT3G26810.
GeneIDi822296.
KEGGiath:AT3G26810.

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016889 Genomic DNA. Translation: BAB01228.1.
CP002686 Genomic DNA. Translation: AEE77218.1.
AY062568 mRNA. Translation: AAL32646.1.
BT010396 mRNA. Translation: AAQ56839.1.
RefSeqiNP_566800.1. NM_113593.2.
UniGeneiAt.6031.

3D structure databases

ProteinModelPortaliQ9LW29.
SMRiQ9LW29. Positions 5-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7626. 13 interactions.
DIPiDIP-34608N.
IntActiQ9LW29. 2 interactions.
STRINGi3702.AT3G26810.1.

Proteomic databases

PaxDbiQ9LW29.
PRIDEiQ9LW29.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G26810.1; AT3G26810.1; AT3G26810.
GeneIDi822296.
KEGGiath:AT3G26810.

Organism-specific databases

TAIRiAT3G26810.

Phylogenomic databases

eggNOGiNOG259467.
HOGENOMiHOG000239805.
InParanoidiQ9LW29.
OMAiARLMACA.
PhylomeDBiQ9LW29.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9LW29.

Family and domain databases

InterProiIPR001810. F-box_dom.
[Graphical view]
PfamiPF00646. F-box. 1 hit.
[Graphical view]
SMARTiSM00256. FBOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression."
    Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.
    Plant Mol. Biol. 49:69-79(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: LEUCINE-RICH REPEATS.
  5. "Plant development is regulated by a family of auxin receptor F box proteins."
    Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
    Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH IAA7.
  6. "The F-box protein TIR1 is an auxin receptor."
    Dharmasiri N., Dharmasiri S., Estelle M.
    Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
    Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
    Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiAFB2_ARATH
AccessioniPrimary (citable) accession number: Q9LW29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.