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Q9LW29

- AFB2_ARATH

UniProt

Q9LW29 - AFB2_ARATH

Protein

Protein AUXIN SIGNALING F-BOX 2

Gene

AFB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Confers sensitivity to the virulent bacterial pathogen P.syringae By similarity. Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Involved in embryogenesis regulation by auxin.By similarity3 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691Myo-inositol hexakisphosphateBy similarity
    Sitei134 – 1341Interaction with auxin-responsive proteinsBy similarity
    Sitei160 – 1601Interaction with auxin-responsive proteinsBy similarity
    Binding sitei340 – 3401Myo-inositol hexakisphosphateBy similarity
    Sitei375 – 3751Interaction with auxin-responsive proteinsBy similarity
    Binding sitei431 – 4311Myo-inositol hexakisphosphateBy similarity
    Sitei484 – 4841Interaction with auxin-responsive proteinsBy similarity
    Binding sitei504 – 5041Myo-inositol hexakisphosphateBy similarity

    GO - Molecular functioni

    1. auxin binding Source: UniProtKB
    2. inositol hexakisphosphate binding Source: UniProtKB

    GO - Biological processi

    1. auxin-activated signaling pathway Source: UniProtKB
    2. defense response Source: UniProtKB-KW
    3. pollen maturation Source: TAIR
    4. protein ubiquitination Source: UniProtKB-UniPathway
    5. response to molecule of bacterial origin Source: TAIR
    6. stamen development Source: TAIR

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Auxin signaling pathway, Plant defense, Ubl conjugation pathway

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein AUXIN SIGNALING F-BOX 2
    Gene namesi
    Name:AFB2
    Synonyms:LRF2
    Ordered Locus Names:At3g26810
    ORF Names:MDJ14.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G26810.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. SCF ubiquitin ligase complex Source: UniProtKB
    3. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575Protein AUXIN SIGNALING F-BOX 2PRO_0000272243Add
    BLAST

    Proteomic databases

    PaxDbiQ9LW29.
    PRIDEiQ9LW29.

    Expressioni

    Tissue specificityi

    Ubiquitous, with higer levels in seedlings.1 Publication

    Inductioni

    Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).1 Publication

    Gene expression databases

    GenevestigatoriQ9LW29.

    Interactioni

    Subunit structurei

    Part of a SCF (SKP1-cullin-F-box) protein ligase complex By similarity. Interacts with Aux/IAA proteins (IAA7) in an auxin-dependent manner.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi7626. 13 interactions.
    DIPiDIP-34608N.
    IntActiQ9LW29. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LW29.
    SMRiQ9LW29. Positions 5-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 4747F-boxAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 772Interaction with auxin-responsive proteinsBy similarity
    Regioni108 – 1092Myo-inositol hexakisphosphate bindingBy similarity
    Regioni343 – 3486Interaction with auxin-responsive proteinsBy similarity
    Regioni396 – 3983Myo-inositol hexakisphosphate bindingBy similarity
    Regioni400 – 4045Interaction with auxin-responsive proteinsBy similarity
    Regioni459 – 4602Interaction with auxin-responsive proteinsBy similarity
    Regioni479 – 4802Myo-inositol hexakisphosphate bindingBy similarity

    Domaini

    The F-box is necessary for the interaction with SKP1.By similarity

    Sequence similaritiesi

    Contains 1 F-box domain.Curated

    Phylogenomic databases

    eggNOGiNOG259467.
    HOGENOMiHOG000239805.
    InParanoidiQ9LW29.
    OMAiAIVRECK.
    PhylomeDBiQ9LW29.

    Family and domain databases

    InterProiIPR001810. F-box_dom.
    [Graphical view]
    PfamiPF00646. F-box. 1 hit.
    [Graphical view]
    SMARTiSM00256. FBOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LW29-1 [UniParc]FASTAAdd to Basket

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    MNYFPDEVIE HVFDFVTSHK DRNAISLVCK SWYKIERYSR QKVFIGNCYA    50
    INPERLLRRF PCLKSLTLKG KPHFADFNLV PHEWGGFVLP WIEALARSRV 100
    GLEELRLKRM VVTDESLELL SRSFVNFKSL VLVSCEGFTT DGLASIAANC 150
    RHLRDLDLQE NEIDDHRGQW LSCFPDTCTT LVTLNFACLE GETNLVALER 200
    LVARSPNLKS LKLNRAVPLD ALARLMACAP QIVDLGVGSY ENDPDSESYL 250
    KLMAVIKKCT SLRSLSGFLE AAPHCLSAFH PICHNLTSLN LSYAAEIHGS 300
    HLIKLIQHCK KLQRLWILDS IGDKGLEVVA STCKELQELR VFPSDLLGGG 350
    NTAVTEEGLV AISAGCPKLH SILYFCQQMT NAALVTVAKN CPNFIRFRLC 400
    ILEPNKPDHV TSQPLDEGFG AIVKACKSLR RLSLSGLLTD QVFLYIGMYA 450
    NQLEMLSIAF AGDTDKGMLY VLNGCKKMKK LEIRDSPFGD TALLADVSKY 500
    ETMRSLWMSS CEVTLSGCKR LAEKAPWLNV EIINENDNNR MEENGHEGRQ 550
    KVDKLYLYRT VVGTRMDAPP FVWIL 575
    Length:575
    Mass (Da):64,605
    Last modified:October 1, 2000 - v1
    Checksum:iD383C4E0A76D50D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB016889 Genomic DNA. Translation: BAB01228.1.
    CP002686 Genomic DNA. Translation: AEE77218.1.
    AY062568 mRNA. Translation: AAL32646.1.
    BT010396 mRNA. Translation: AAQ56839.1.
    RefSeqiNP_566800.1. NM_113593.2.
    UniGeneiAt.6031.

    Genome annotation databases

    EnsemblPlantsiAT3G26810.1; AT3G26810.1; AT3G26810.
    GeneIDi822296.
    KEGGiath:AT3G26810.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB016889 Genomic DNA. Translation: BAB01228.1 .
    CP002686 Genomic DNA. Translation: AEE77218.1 .
    AY062568 mRNA. Translation: AAL32646.1 .
    BT010396 mRNA. Translation: AAQ56839.1 .
    RefSeqi NP_566800.1. NM_113593.2.
    UniGenei At.6031.

    3D structure databases

    ProteinModelPortali Q9LW29.
    SMRi Q9LW29. Positions 5-573.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 7626. 13 interactions.
    DIPi DIP-34608N.
    IntActi Q9LW29. 2 interactions.

    Proteomic databases

    PaxDbi Q9LW29.
    PRIDEi Q9LW29.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G26810.1 ; AT3G26810.1 ; AT3G26810 .
    GeneIDi 822296.
    KEGGi ath:AT3G26810.

    Organism-specific databases

    TAIRi AT3G26810.

    Phylogenomic databases

    eggNOGi NOG259467.
    HOGENOMi HOG000239805.
    InParanoidi Q9LW29.
    OMAi AIVRECK.
    PhylomeDBi Q9LW29.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Gene expression databases

    Genevestigatori Q9LW29.

    Family and domain databases

    InterProi IPR001810. F-box_dom.
    [Graphical view ]
    Pfami PF00646. F-box. 1 hit.
    [Graphical view ]
    SMARTi SM00256. FBOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Cloning by pathway activation in yeast: identification of an Arabidopsis thaliana F-box protein that can turn on glucose repression."
      Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.
      Plant Mol. Biol. 49:69-79(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: LEUCINE-RICH REPEATS.
    5. "Plant development is regulated by a family of auxin receptor F box proteins."
      Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M., Hobbie L., Ehrismann J.S., Juergens G., Estelle M.
      Dev. Cell 9:109-119(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH IAA7.
    6. "The F-box protein TIR1 is an auxin receptor."
      Dharmasiri N., Dharmasiri S., Estelle M.
      Nature 435:441-445(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A plant miRNA contributes to antibacterial resistance by repressing auxin signaling."
      Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G.
      Science 312:436-439(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiAFB2_ARATH
    AccessioniPrimary (citable) accession number: Q9LW29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3