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Protein

Bifunctional dihydrocamalexate synthase/camalexin synthase

Gene

CYP71B15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional enzyme involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes two reactions, the formation of dihydrocamalexate from indole-3-acetonitrile-cysteine conjugate and the oxidative decarboxylation of dihydrocamalexate which is the final step in camalexin biosynthesis. Required for the resistance to the fungal pathogens A.brassicicola, B.cinerea, B.elliptica, B.tulipae, L.maculans and Colletotrichum higginsianum. Seems not to be required for resistance to P.syringae, P.porri, and not involved in age-related resistance.14 Publications

Catalytic activityi

2-(L-cystein-S-yl)-2-(1H-indol-3-yl)acetonitrile + 2 [reduced NADPH--hemoprotein reductase] + 2 O2 = camalexin + hydrogen cyanide + CO2 + 2 [oxidized NADPH--hemoprotein reductase] + 4 H2O.2 Publications

GO - Molecular functioni

GO - Biological processi

  • camalexin biosynthetic process Source: TAIR
  • defense response Source: TAIR
  • defense response to fungus Source: TAIR
  • indole phytoalexin biosynthetic process Source: TAIR
  • regulation of systemic acquired resistance Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to bacterium Source: TAIR
  • response to insect Source: TAIR
  • response to water deprivation Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processPlant defense
LigandNADP

Enzyme and pathway databases

BioCyciARA:AT3G26830-MONOMER
MetaCyc:AT3G26830-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrocamalexate synthase/camalexin synthase (EC:1.14.19.522 Publications)
Alternative name(s):
Cytochrome P450 71B15
Dihydrocamalexate:NADP(+) oxidoreductase (decarboxylating)
Protein PHYTOALEXIN DEFICIENT 3
Gene namesi
Name:CYP71B15
Synonyms:PAD3
Ordered Locus Names:At3g26830
ORF Names:MDJ14.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G26830
TAIRilocus:2088394 AT3G26830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but deficient in the phytoalexin camalexin accumulation upon bacterial infection and markedly increased susceptibility to infection by the necrotrophic fungus Alternaria brassicicola.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176G → E in pad3-2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000520931 – 490Bifunctional dihydrocamalexate synthase/camalexin synthaseAdd BLAST490

Proteomic databases

PaxDbiQ9LW27

Expressioni

Inductioni

By salicylic acid, flagellin, oligogalacturonides, silver nitrate, UV-C and infection with A.alternata and various strains of P.syringae. Activated by the transcription factor WRKY33 upon infection with P.syringae.8 Publications

Gene expression databases

ExpressionAtlasiQ9LW27 baseline and differential
GenevisibleiQ9LW27 AT

Interactioni

Protein-protein interaction databases

BioGridi7628, 3 interactors
STRINGi3702.AT3G26830.1

Structurei

3D structure databases

ProteinModelPortaliQ9LW27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOGENOMiHOG000218629
InParanoidiQ9LW27
KOiK20559
OMAiINDLECC
OrthoDBiEOG093613M8
PhylomeDBiQ9LW27

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LW27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFLCFLVL LPLILIFLNV LKPSKYKLPP GPKKLPIIGN LHQRRTLHPR
60 70 80 90 100
NRRNLAEMYG PVALLQYGFV PVVAISSKEA AEEVLKINDL ECCSRPEAAG
110 120 130 140 150
MRATFYNFKD IGMAPFGDEW SLMRKLSVVE LFSVKKLQSF KYIIEEENNL
160 170 180 190 200
CVKKLSEFAT RQSPVNLERA IFTLVGNIVC RIGYGINLYE CDFFEADRVV
210 220 230 240 250
DLVLKAEAVI RETVFSDFFP GRIGRFIDCI SGQNRRLKNN FSVVDTFFQN
260 270 280 290 300
VLNEHLKPGR ESSTIVDLMI DMKKKQENDG DALKFTTDHL KGMISDIFVA
310 320 330 340 350
GIGGVAGITL WGMTELIRNP RVMKKVQDEI RTTLGDKKER IKEEDLNQLH
360 370 380 390 400
YFKLVVKETL RLHPTTPLLL PRQTMSHIKI QGYDVPAKTQ ILVNVYAMGR
410 420 430 440 450
DPKLWENADE FNPDRFLDSS VDFKGKNYEF IPFGSGRRIC PGMTMGTILV
460 470 480 490
EMALLNLLYF FDWGLAKQEE AKEIINGEEN FLAFFQVLHH
Length:490
Mass (Da):56,061
Last modified:October 1, 2000 - v1
Checksum:iB8F84D85F252668D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016889 Genomic DNA Translation: BAB01230.1
CP002686 Genomic DNA Translation: AEE77220.1
AK117967 mRNA Translation: BAC42604.1
RefSeqiNP_189318.1, NM_113595.4
UniGeneiAt.6033

Genome annotation databases

EnsemblPlantsiAT3G26830.1; AT3G26830.1; AT3G26830
GeneIDi822298
GrameneiAT3G26830.1; AT3G26830.1; AT3G26830
KEGGiath:AT3G26830

Similar proteinsi

Entry informationi

Entry nameiC71BF_ARATH
AccessioniPrimary (citable) accession number: Q9LW27
Secondary accession number(s): Q541X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health