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Protein

Bifunctional dihydrocamalexate synthase/camalexin synthase

Gene

CYP71B15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes two reactions, the formation of dihydrocamalexate from indole-3-acetonitrile-cysteine conjugate and the oxidative decarboxylation of dihydrocamalexate which is the final step in camalexin biosynthesis. Required for the resistance to the fungal pathogens A.brassicicola, B.cinerea, B.elliptica, B.tulipae, L.maculans and Colletotrichum higginsianum. Seems not to be required for resistance to P.syringae, P.porri, and not involved in age-related resistance.14 Publications

Catalytic activityi

Indole-3-acetonitrile-cysteine conjugate + NADPH = dihydrocamalexate + hydrogen cyanide + NADP+.2 Publications
Dihydrocamalexate + NADP+ = camalexin + CO2 + NADPH.2 Publications

GO - Molecular functioni

GO - Biological processi

  • camalexin biosynthetic process Source: TAIR
  • defense response Source: TAIR
  • defense response to fungus Source: TAIR
  • indole phytoalexin biosynthetic process Source: TAIR
  • regulation of systemic acquired resistance Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to bacterium Source: TAIR
  • response to insect Source: TAIR
  • response to water deprivation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT3G26830-MONOMER.
MetaCyc:AT3G26830-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydrocamalexate synthase/camalexin synthase (EC:1.3.1.n22 Publications)
Alternative name(s):
Cytochrome P450 71B15
Dihydrocamalexate:NADP(+) oxidoreductase (decarboxylating)
Protein PHYTOALEXIN DEFICIENT 3
Gene namesi
Name:CYP71B15
Synonyms:PAD3
Ordered Locus Names:At3g26830
ORF Names:MDJ14.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G26830.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: TAIR
  • membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but deficient in the phytoalexin camalexin accumulation upon bacterial infection and markedly increased susceptibility to infection by the necrotrophic fungus Alternaria brassicicola.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi176 – 1761G → E in pad3-2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Bifunctional dihydrocamalexate synthase/camalexin synthasePRO_0000052093Add
BLAST

Proteomic databases

PaxDbiQ9LW27.
PRIDEiQ9LW27.

Expressioni

Inductioni

By salicylic acid, flagellin, oligogalacturonides, silver nitrate, UV-C and infection with A.alternata and various strains of P.syringae. Activated by the transcription factor WRKY33 upon infection with P.syringae.8 Publications

Gene expression databases

GenevisibleiQ9LW27. AT.

Interactioni

Protein-protein interaction databases

BioGridi7628. 3 interactions.
STRINGi3702.AT3G26830.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LW27.
SMRiQ9LW27. Positions 27-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218629.
InParanoidiQ9LW27.
KOiK00517.
OMAiINDLECC.
PhylomeDBiQ9LW27.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LW27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFLCFLVL LPLILIFLNV LKPSKYKLPP GPKKLPIIGN LHQRRTLHPR
60 70 80 90 100
NRRNLAEMYG PVALLQYGFV PVVAISSKEA AEEVLKINDL ECCSRPEAAG
110 120 130 140 150
MRATFYNFKD IGMAPFGDEW SLMRKLSVVE LFSVKKLQSF KYIIEEENNL
160 170 180 190 200
CVKKLSEFAT RQSPVNLERA IFTLVGNIVC RIGYGINLYE CDFFEADRVV
210 220 230 240 250
DLVLKAEAVI RETVFSDFFP GRIGRFIDCI SGQNRRLKNN FSVVDTFFQN
260 270 280 290 300
VLNEHLKPGR ESSTIVDLMI DMKKKQENDG DALKFTTDHL KGMISDIFVA
310 320 330 340 350
GIGGVAGITL WGMTELIRNP RVMKKVQDEI RTTLGDKKER IKEEDLNQLH
360 370 380 390 400
YFKLVVKETL RLHPTTPLLL PRQTMSHIKI QGYDVPAKTQ ILVNVYAMGR
410 420 430 440 450
DPKLWENADE FNPDRFLDSS VDFKGKNYEF IPFGSGRRIC PGMTMGTILV
460 470 480 490
EMALLNLLYF FDWGLAKQEE AKEIINGEEN FLAFFQVLHH
Length:490
Mass (Da):56,061
Last modified:October 1, 2000 - v1
Checksum:iB8F84D85F252668D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016889 Genomic DNA. Translation: BAB01230.1.
CP002686 Genomic DNA. Translation: AEE77220.1.
AK117967 mRNA. Translation: BAC42604.1.
RefSeqiNP_189318.1. NM_113595.3.
UniGeneiAt.6033.

Genome annotation databases

EnsemblPlantsiAT3G26830.1; AT3G26830.1; AT3G26830.
GeneIDi822298.
GrameneiAT3G26830.1; AT3G26830.1; AT3G26830.
KEGGiath:AT3G26830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016889 Genomic DNA. Translation: BAB01230.1.
CP002686 Genomic DNA. Translation: AEE77220.1.
AK117967 mRNA. Translation: BAC42604.1.
RefSeqiNP_189318.1. NM_113595.3.
UniGeneiAt.6033.

3D structure databases

ProteinModelPortaliQ9LW27.
SMRiQ9LW27. Positions 27-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7628. 3 interactions.
STRINGi3702.AT3G26830.1.

Proteomic databases

PaxDbiQ9LW27.
PRIDEiQ9LW27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G26830.1; AT3G26830.1; AT3G26830.
GeneIDi822298.
GrameneiAT3G26830.1; AT3G26830.1; AT3G26830.
KEGGiath:AT3G26830.

Organism-specific databases

TAIRiAT3G26830.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218629.
InParanoidiQ9LW27.
KOiK00517.
OMAiINDLECC.
PhylomeDBiQ9LW27.

Enzyme and pathway databases

BioCyciARA:AT3G26830-MONOMER.
MetaCyc:AT3G26830-MONOMER.

Miscellaneous databases

PROiQ9LW27.

Gene expression databases

GenevisibleiQ9LW27. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Isolation of phytoalexin-deficient mutants of Arabidopsis thaliana and characterization of their interactions with bacterial pathogens."
    Glazebrook J., Ausubel F.M.
    Proc. Natl. Acad. Sci. U.S.A. 91:8955-8959(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Deficiency in phytoalexin production causes enhanced susceptibility of Arabidopsis thaliana to the fungus Alternaria brassicicola."
    Thomma B.P., Nelissen I., Eggermont K., Broekaert W.F.
    Plant J. 19:163-171(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Arabidopsis PAD3, a gene required for camalexin biosynthesis, encodes a putative cytochrome P450 monooxygenase."
    Zhou N., Tootle T.L., Glazebrook J.
    Plant Cell 11:2419-2428(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF GLY-176.
  7. "Characterization of an Arabidopsis-Phytophthora pathosystem: resistance requires a functional PAD2 gene and is independent of salicylic acid, ethylene and jasmonic acid signalling."
    Roetschi A., Si-Ammour A., Belbahri L., Mauch F., Mauch-Mani B.
    Plant J. 28:293-305(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Age-related resistance in Arabidopsis is a developmentally regulated defense response to Pseudomonas syringae."
    Kus J.V., Zaton K., Sarkar R., Cameron R.K.
    Plant Cell 14:479-490(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Arabidopsis local resistance to Botrytis cinerea involves salicylic acid and camalexin and requires EDS4 and PAD2, but not SID2, EDS5 or PAD4."
    Ferrari S., Plotnikova J.M., De Lorenzo G., Ausubel F.M.
    Plant J. 35:193-205(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  10. "Characterisation of an Arabidopsis-Leptosphaeria maculans pathosystem: resistance partially requires camalexin biosynthesis and is independent of salicylic acid, ethylene and jasmonic acid signalling."
    Bohman S., Staal J., Thomma B.P., Wang M., Dixelius C.
    Plant J. 37:9-20(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Regulatory variability of camalexin biosynthesis."
    Schuhegger R., Rauhut T., Glawischnig E.
    J. Plant Physiol. 164:636-644(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
  13. "Histochemical and genetic analysis of host and non-host interactions of Arabidopsis with three Botrytis species: an important role for cell death control."
    van Baarlen P., Woltering E.J., Staats M., van Kan J.A.
    Mol. Plant Pathol. 8:41-54(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Arabidopsis cytochrome P450 monooxygenase 71A13 catalyzes the conversion of indole-3-acetaldoxime in camalexin synthesis."
    Nafisi M., Goregaoker S., Botanga C.J., Glawischnig E., Olsen C.E., Halkier B.A., Glazebrook J.
    Plant Cell 19:2039-2052(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  15. "Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid, ethylene, or jasmonate signaling but requires PHYTOALEXIN DEFICIENT3."
    Ferrari S., Galletti R., Denoux C., De Lorenzo G., Ausubel F.M., Dewdney J.
    Plant Physiol. 144:367-379(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  16. Cited for: INDUCTION.
  17. "The AtrbohD-mediated oxidative burst elicited by oligogalacturonides in Arabidopsis is dispensable for the activation of defense responses effective against Botrytis cinerea."
    Galletti R., Denoux C., Gambetta S., Dewdney J., Ausubel F.M., De Lorenzo G., Ferrari S.
    Plant Physiol. 148:1695-1706(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OLIGOGALACTURONIDES.
  18. "Glycerol-3-phosphate levels are associated with basal resistance to the hemibiotrophic fungus Colletotrichum higginsianum in Arabidopsis."
    Chanda B., Venugopal S.C., Kulshrestha S., Navarre D.A., Downie B., Vaillancourt L., Kachroo A., Kachroo P.
    Plant Physiol. 147:2017-2029(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "The multifunctional enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) converts cysteine-indole-3-acetonitrile to camalexin in the indole-3-acetonitrile metabolic network of Arabidopsis thaliana."
    Boettcher C., Westphal L., Schmotz C., Prade E., Scheel D., Glawischnig E.
    Plant Cell 21:1830-1845(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  20. "The ABC transporter BcatrB from Botrytis cinerea exports camalexin and is a virulence factor on Arabidopsis thaliana."
    Stefanato F.L., Abou-Mansour E., Buchala A., Kretschmer M., Mosbach A., Hahn M., Bochet C.G., Metraux J.P., Schoonbeek H.J.
    Plant J. 58:499-510(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.

Entry informationi

Entry nameiC71BF_ARATH
AccessioniPrimary (citable) accession number: Q9LW27
Secondary accession number(s): Q541X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.