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Protein

Probable polygalacturonase At3g15720

Gene

At3g15720

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei210 – 2101Proton donorPROSITE-ProRule annotation
Active sitei233 – 2331PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G15720-MONOMER.
ARA:GQT-1927-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable polygalacturonase At3g15720 (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Pectinase At3g15720
Gene namesi
Ordered Locus Names:At3g15720
ORF Names:MSJ11.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G15720.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • extracellular region Source: UniProtKB-KW
  • plant-type cell wall Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 430407Probable polygalacturonase At3g15720PRO_0000259446Add
BLAST
Propeptidei431 – 45626Removed in mature formSequence analysisPRO_0000259447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi430 – 4301GPI-anchor amidated serineSequence analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9LW07.
PRIDEiQ9LW07.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LW07. baseline and differential.
GenevisibleiQ9LW07. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G15720.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LW07.
SMRiQ9LW07. Positions 24-360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati169 – 19527PbH1 1Add
BLAST
Repeati196 – 21722PbH1 2Add
BLAST
Repeati219 – 23921PbH1 3Add
BLAST
Repeati249 – 27022PbH1 4Add
BLAST
Repeati278 – 29922PbH1 5Add
BLAST
Repeati314 – 34128PbH1 6Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IK0F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ9LW07.
KOiK01184.
OMAiGVDFRCS.
OrthoDBiEOG09360AGW.
PhylomeDBiQ9LW07.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LW07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKKTWFLNF SLFFLQIFTS SNALDVTQFG AVGDGVTDDS QAFLKAWEAV
60 70 80 90 100
CSGTGDGQFV VPAGMTFMLQ PLKFQGSCKS TPVFVQMLGK LVAPSKGNWK
110 120 130 140 150
GDKDQWILFT DIEGLVIEGD GEINGQGSSW WEHKGSRPTA LKFRSCNNLR
160 170 180 190 200
LSGLTHLDSP MAHIHISECN YVTISSLRIN APESSPNTDG IDVGASSNVV
210 220 230 240 250
IQDCIIATGD DCIAINSGTS NIHISGIDCG PGHGISIGSL GKDGETATVE
260 270 280 290 300
NVCVQNCNFR GTMNGARIKT WQGGSGYARM ITFNGITLDN VENPIIIDQF
310 320 330 340 350
YNGGDSDNAK DRKSSAVEVS KVVFSNFIGT SKSEYGVDFR CSERVPCTEI
360 370 380 390 400
FLRDMKIETA SSGSGQVAQG QCLNVRGAST IAVPGLECLE LSTDMFSSAQ
410 420 430 440 450
LLEQTCMSAQ SVQPRTTTQP MQDPIWVFQS RGKQLRVYNI AILVSFISLV

TYILAR
Length:456
Mass (Da):49,300
Last modified:October 1, 2000 - v1
Checksum:iE85AFE25CACD5CBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017071 Genomic DNA. Translation: BAB02303.1.
CP002686 Genomic DNA. Translation: AEE75718.1.
RefSeqiNP_566524.1. NM_112442.1. [Q9LW07-1]
UniGeneiAt.39003.

Genome annotation databases

EnsemblPlantsiAT3G15720.1; AT3G15720.1; AT3G15720. [Q9LW07-1]
GeneIDi820815.
KEGGiath:AT3G15720.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017071 Genomic DNA. Translation: BAB02303.1.
CP002686 Genomic DNA. Translation: AEE75718.1.
RefSeqiNP_566524.1. NM_112442.1. [Q9LW07-1]
UniGeneiAt.39003.

3D structure databases

ProteinModelPortaliQ9LW07.
SMRiQ9LW07. Positions 24-360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G15720.1.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PaxDbiQ9LW07.
PRIDEiQ9LW07.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G15720.1; AT3G15720.1; AT3G15720. [Q9LW07-1]
GeneIDi820815.
KEGGiath:AT3G15720.

Organism-specific databases

TAIRiAT3G15720.

Phylogenomic databases

eggNOGiENOG410IK0F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ9LW07.
KOiK01184.
OMAiGVDFRCS.
OrthoDBiEOG09360AGW.
PhylomeDBiQ9LW07.

Enzyme and pathway databases

BioCyciARA:AT3G15720-MONOMER.
ARA:GQT-1927-MONOMER.

Miscellaneous databases

PROiQ9LW07.

Gene expression databases

ExpressionAtlasiQ9LW07. baseline and differential.
GenevisibleiQ9LW07. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR3_ARATH
AccessioniPrimary (citable) accession number: Q9LW07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.