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Protein

Probable polygalacturonase At3g15720

Gene

At3g15720

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei210Proton donorPROSITE-ProRule annotation1
Active sitei233PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G15720-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable polygalacturonase At3g15720 (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
Pectinase At3g15720
Gene namesi
Ordered Locus Names:At3g15720
ORF Names:MSJ11.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G15720.
TAIRilocus:2093212. AT3G15720.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: TAIR
  • anchored component of plasma membrane Source: TAIR
  • extracellular region Source: UniProtKB-KW
  • plant-type cell wall Source: TAIR

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000025944624 – 430Probable polygalacturonase At3g15720Add BLAST407
PropeptideiPRO_0000259447431 – 456Removed in mature formSequence analysisAdd BLAST26

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi430GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9LW07.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LW07. baseline and differential.
GenevisibleiQ9LW07. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G15720.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LW07.
SMRiQ9LW07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati169 – 195PbH1 1Add BLAST27
Repeati196 – 217PbH1 2Add BLAST22
Repeati219 – 239PbH1 3Add BLAST21
Repeati249 – 270PbH1 4Add BLAST22
Repeati278 – 299PbH1 5Add BLAST22
Repeati314 – 341PbH1 6Add BLAST28

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IK0F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiQ9LW07.
KOiK01184.
OMAiTFMLQPL.
OrthoDBiEOG09360AGW.
PhylomeDBiQ9LW07.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiView protein in InterPro
IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
PfamiView protein in Pfam
PF00295. Glyco_hydro_28. 1 hit.
SMARTiView protein in SMART
SM00710. PbH1. 6 hits.
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiView protein in PROSITE
PS00502. POLYGALACTURONASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LW07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKKTWFLNF SLFFLQIFTS SNALDVTQFG AVGDGVTDDS QAFLKAWEAV
60 70 80 90 100
CSGTGDGQFV VPAGMTFMLQ PLKFQGSCKS TPVFVQMLGK LVAPSKGNWK
110 120 130 140 150
GDKDQWILFT DIEGLVIEGD GEINGQGSSW WEHKGSRPTA LKFRSCNNLR
160 170 180 190 200
LSGLTHLDSP MAHIHISECN YVTISSLRIN APESSPNTDG IDVGASSNVV
210 220 230 240 250
IQDCIIATGD DCIAINSGTS NIHISGIDCG PGHGISIGSL GKDGETATVE
260 270 280 290 300
NVCVQNCNFR GTMNGARIKT WQGGSGYARM ITFNGITLDN VENPIIIDQF
310 320 330 340 350
YNGGDSDNAK DRKSSAVEVS KVVFSNFIGT SKSEYGVDFR CSERVPCTEI
360 370 380 390 400
FLRDMKIETA SSGSGQVAQG QCLNVRGAST IAVPGLECLE LSTDMFSSAQ
410 420 430 440 450
LLEQTCMSAQ SVQPRTTTQP MQDPIWVFQS RGKQLRVYNI AILVSFISLV

TYILAR
Length:456
Mass (Da):49,300
Last modified:October 1, 2000 - v1
Checksum:iE85AFE25CACD5CBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017071 Genomic DNA. Translation: BAB02303.1.
CP002686 Genomic DNA. Translation: AEE75718.1.
RefSeqiNP_566524.1. NM_112442.2. [Q9LW07-1]
UniGeneiAt.39003.

Genome annotation databases

EnsemblPlantsiAT3G15720.1; AT3G15720.1; AT3G15720. [Q9LW07-1]
GeneIDi820815.
GrameneiAT3G15720.1; AT3G15720.1; AT3G15720.
KEGGiath:AT3G15720.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPGLR3_ARATH
AccessioniPrimary (citable) accession number: Q9LW07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families