Q9LW07 (PGLR3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable polygalacturonase At3g15720
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase At3g15720

Gene names

Ordered Locus Names:	At3g15720
ORF Names:	MSJ11.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor. Secreted › cell wall Ref.3.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cell wall Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to plasma membrane Inferred from direct assay Ref.3. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LW07-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 430	407	Probable polygalacturonase At3g15720		PRO_0000259446
	Propeptide	431 – 456	26	Removed in mature form Potential		PRO_0000259447
Regions
	Repeat	169 – 195	27	PbH1 1
	Repeat	196 – 217	22	PbH1 2
	Repeat	219 – 239	21	PbH1 3
	Repeat	249 – 270	22	PbH1 4
	Repeat	278 – 299	22	PbH1 5
	Repeat	314 – 341	28	PbH1 6
Sites
	Active site	210	1	Proton donor By similarity
	Active site	233	1	By similarity
Amino acid modifications
	Lipidation	430	1	GPI-anchor amidated serine Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E85AFE25CACD5CBD	FASTA	456	49,300
	10 20 30 40 50 60 MKKKTWFLNF SLFFLQIFTS SNALDVTQFG AVGDGVTDDS QAFLKAWEAV CSGTGDGQFV 70 80 90 100 110 120 VPAGMTFMLQ PLKFQGSCKS TPVFVQMLGK LVAPSKGNWK GDKDQWILFT DIEGLVIEGD 130 140 150 160 170 180 GEINGQGSSW WEHKGSRPTA LKFRSCNNLR LSGLTHLDSP MAHIHISECN YVTISSLRIN 190 200 210 220 230 240 APESSPNTDG IDVGASSNVV IQDCIIATGD DCIAINSGTS NIHISGIDCG PGHGISIGSL 250 260 270 280 290 300 GKDGETATVE NVCVQNCNFR GTMNGARIKT WQGGSGYARM ITFNGITLDN VENPIIIDQF 310 320 330 340 350 360 YNGGDSDNAK DRKSSAVEVS KVVFSNFIGT SKSEYGVDFR CSERVPCTEI FLRDMKIETA 370 380 390 400 410 420 SSGSGQVAQG QCLNVRGAST IAVPGLECLE LSTDMFSSAQ LLEQTCMSAQ SVQPRTTTQP 430 440 450 MQDPIWVFQS RGKQLRVYNI AILVSFISLV TYILAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Proteomic analysis of glycosylphosphatidylinositol-anchored membrane proteins." Elortza F., Nuehse T.S., Foster L.J., Stensballe A., Peck S.C., Jensen O.N. Mol. Cell. Proteomics 2:1261-1270(2003) [PubMed: 14517339] [Abstract] Cited for: GPI-ANCHOR [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017071 Genomic DNA. Translation: BAB02303.1. CP002686 Genomic DNA. Translation: AEE75718.1.
IPI	IPI00519614.
RefSeq	NP_566524.1. NM_112442.1.
UniGene	At.39003.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ProteinModelPortal	Q9LW07.
SMR	Q9LW07. Positions 19-378.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LW07.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	Q9LW07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G15720.1; AT3G15720.1; AT3G15720.
GeneID	820815.
GenomeReviews	Gene locus AT3G15720 in contig BA000014_GR.
KEGG	ath:AT3G15720.
NMPDR	fig|3702.1.peg.13738.
Organism-specific databases
GeneFarm	340. 2.
TAIR	At3g15720.
Phylogenomic databases
GeneTree	EPGT00070000028548.
HOGENOM	HBG744658.
InParanoid	Q9LW07.
OMA	SLRINAP.
PhylomeDB	Q9LW07.
ProtClustDB	PLN03003.
Gene expression databases
Genevestigator	Q9LW07.
GermOnline	AT3G15720. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01184.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR3_ARATH

Accession

Primary (citable) accession number: Q9LW07

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program