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Protein

Tirucalladienol synthase

Gene

PEN3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts oxidosqualene to tirucalladienol.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = tirucalla-7,24-dien-3-beta-ol.1 Publication

GO - Molecular functioni

  • lupeol synthase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT5G36150-MONOMER.
MetaCyc:AT5G36150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tirucalladienol synthase (EC:5.4.99.56)
Alternative name(s):
Pentacyclic triterpene synthase 3
Short name:
AtPEN3
Gene namesi
Name:PEN3
Ordered Locus Names:At5g36150
ORF Names:MAB16.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G36150.

Subcellular locationi

GO - Cellular componenti

  • plasmodesma Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 760760Tirucalladienol synthasePRO_0000366138Add
BLAST

Proteomic databases

PaxDbiQ9LVY2.
PRIDEiQ9LVY2.

PTM databases

iPTMnetiQ9LVY2.

Expressioni

Gene expression databases

GenevisibleiQ9LVY2. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G36150.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LVY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati149 – 19042PFTB 1Add
BLAST
Repeati641 – 68242PFTB 2Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 2 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ9LVY2.
KOiK16205.
PhylomeDBiQ9LVY2.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LVY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWRLRIGAKA GDDPHLCTTN NFLGRQIWEF DANAGSPAEL SEVDQARQNF
60 70 80 90 100
SNNRSQYKAC ADLLWRMQFL REKNFEQKIP RVRIEDAKKI TFEDAKNTLR
110 120 130 140 150
RGIHYMAALQ SDDGHWPSEN AGCIFFNAPF VICLYITGHL DKVFSEEHRK
160 170 180 190 200
EMLRYMYNHQ NDDGGWGIDV ESHSFMFCTV INYICLRIFG VDPDHDGESA
210 220 230 240 250
CARARKWIID HGGATYTPLF GKAWLSVLGV YEWSGCKPIP PEFWFFPSYF
260 270 280 290 300
PINGGTLWIY LRDTFMAMSY LYGKKFVAKP TPLILQLREE LYPQPYAEIV
310 320 330 340 350
WSQARSRCAK EDLYYPQSLV QDLFWKLVHM FSENILNRWP FNKLIREKAI
360 370 380 390 400
RTAMELIHYH DEATRYITGG AVPKVFHMLA CWVEDPESDY FKKHLARVSH
410 420 430 440 450
FIWIAEDGLK IQTFGSQIWD TAFVLQVMLA ADVDDEIRPT LIKGYSYLRK
460 470 480 490 500
SQFTENPPGD YINMFRDISK GGWGYSDKDQ GWPVSDCISE SLECCLIFES
510 520 530 540 550
MSSEFIGEKM EVERLYDAVN MLLYMQSRNG GISIWEAASG KKWLEWLSPI
560 570 580 590 600
EFIEDTILEH EYLECTGSAI VVLARFMKQF PGHRTEEVKK FITKGVKYIE
610 620 630 640 650
SLQIADGSWY GNWGICFIYG TFFAVRGLVA AGNTYDNCEA IRRAVRFLLD
660 670 680 690 700
IQNGEGGWGE SFLSCPNKNY IPLEGNKTDV VNTGQALMVL IMGGQMDRDP
710 720 730 740 750
LPVHRAAKVL INSQMDNGDF PQQEIRGVYK MNVMLNFPTF RNSFTLWALT
760
HYTKAIRLLL
Length:760
Mass (Da):87,804
Last modified:September 22, 2009 - v2
Checksum:iD30F08C6501FCE4B
GO

Sequence cautioni

The sequence BAA96890.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018112 Genomic DNA. Translation: BAA96890.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94049.1.
RefSeqiNP_198464.4. NM_123006.4.
UniGeneiAt.55157.

Genome annotation databases

EnsemblPlantsiAT5G36150.1; AT5G36150.1; AT5G36150.
GeneIDi833612.
GrameneiAT5G36150.1; AT5G36150.1; AT5G36150.
KEGGiath:AT5G36150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018112 Genomic DNA. Translation: BAA96890.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED94049.1.
RefSeqiNP_198464.4. NM_123006.4.
UniGeneiAt.55157.

3D structure databases

ProteinModelPortaliQ9LVY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G36150.1.

PTM databases

iPTMnetiQ9LVY2.

Proteomic databases

PaxDbiQ9LVY2.
PRIDEiQ9LVY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G36150.1; AT5G36150.1; AT5G36150.
GeneIDi833612.
GrameneiAT5G36150.1; AT5G36150.1; AT5G36150.
KEGGiath:AT5G36150.

Organism-specific databases

TAIRiAT5G36150.

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiQ9LVY2.
KOiK16205.
PhylomeDBiQ9LVY2.

Enzyme and pathway databases

BioCyciARA:AT5G36150-MONOMER.
MetaCyc:AT5G36150-MONOMER.

Miscellaneous databases

PROiQ9LVY2.

Gene expression databases

GenevisibleiQ9LVY2. AT.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana."
    Husselstein-Muller T., Schaller H., Benveniste P.
    Plant Mol. Biol. 45:75-92(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, NOMENCLATURE.
  4. "Product profile of PEN3: the last unexamined oxidosqualene cyclase in Arabidopsis thaliana."
    Morlacchi P., Wilson W.K., Xiong Q., Bhaduri A., Sttivend D., Kolesnikova M.D., Matsuda S.P.T.
    Org. Lett. 11:2627-2630(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiPEN3_ARATH
AccessioniPrimary (citable) accession number: Q9LVY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: September 22, 2009
Last modified: June 8, 2016
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.