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Protein

Carbamoyl-phosphate synthase small chain, chloroplastic

Gene

CARA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in arginine biosynthesis. Required for mesophyll development.1 Publication

Miscellaneous

The ven6-1 phenotype is rescued by exogenous application of citrulline, an arginine precursor.1 Publication

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
  2. Aspartate carbamoyltransferase, chloroplastic (PYRB)
  3. Dihydroorotase, mitochondrial (PYR4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei318NucleophileBy similarity1
Active sitei402By similarity1
Active sitei404By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G27740-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Protein family/group databases

MEROPSiC26.A04.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain, chloroplastic (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Protein VENOSA 6
Gene namesi
Name:CARA
Synonyms:VEN6
Ordered Locus Names:At3g27740
ORF Names:MGF10.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2089149. AT3G27740.

Subcellular locationi

GO - Cellular componenti

  • carbamoyl-phosphate synthase complex Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced plant size. Reticulate leaves with reduced number of palissade mesophyll cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi410H → Y in ven6-1; reticulate leaf phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35ChloroplastSequence analysisAdd BLAST35
ChainiPRO_000042307436 – 430Carbamoyl-phosphate synthase small chain, chloroplasticAdd BLAST395

Proteomic databases

PaxDbiQ9LVW7.

Expressioni

Inductioni

By phosphate starvation in shoot.1 Publication

Gene expression databases

ExpressionAtlasiQ9LVW7. baseline and differential.
GenevisibleiQ9LVW7. AT.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

BioGridi7726. 2 interactors.
IntActiQ9LVW7. 1 interactor.
STRINGi3702.AT3G27740.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LVW7.
SMRiQ9LVW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini243 – 429Glutamine amidotransferase type-1Add BLAST187

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Phylogenomic databases

eggNOGiENOG410IUMV. Eukaryota.
COG0505. LUCA.
HOGENOMiHOG000038087.
InParanoidiQ9LVW7.
KOiK01956.
OMAiCFSVQYH.
OrthoDBiEOG093609NY.
PhylomeDBiQ9LVW7.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LVW7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMATRTLGF VLPTSLSSQP SFDRRGGGFR VSVIRCSTSP LTFPTSGVVE
60 70 80 90 100
KPWTSYNARL VLEDGSIWPA KSFGAPGTRI AELVFNTSLT GYQEILTDPS
110 120 130 140 150
YAGQFVLMTN PQIGNTGVNP DDEESGQCFL TGLVIRNLSI STSNWRCTKT
160 170 180 190 200
LADYLTERDI MGVYDLDTRA ITRRLREDGS LIGVLSTEQS KTDDELLQMS
210 220 230 240 250
RSWDIVGIDL ISDVSCKSPY EWVDKTNAEW DFNTNSRDGK SYKVIAYDFG
260 270 280 290 300
IKQNILRRLS SYGCQITVVP STFPAAEALK MNPDGILFSN GPGDPSAVPY
310 320 330 340 350
AVETVKELLG KVPVYGICMG HQLLGQALGG KTFKMKFGHH GGNHPVRNNR
360 370 380 390 400
TGQVEISAQN HNYAVDPASL PGGVEVTHVN LNDGSCAGLS FPEMNVMSLQ
410 420 430
YHPEASPGPH DSDNAFREFI ELMKRSKQSS
Length:430
Mass (Da):47,059
Last modified:October 1, 2000 - v1
Checksum:i25E615DB33D26ECC
GO

Sequence cautioni

The sequence AAC25961 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018114 Genomic DNA. Translation: BAB02698.1.
CP002686 Genomic DNA. Translation: AEE77358.1.
AY046004 mRNA. Translation: AAK76678.1.
AY079315 mRNA. Translation: AAL85046.1.
U73175 mRNA. Translation: AAC25961.1. Different initiation.
RefSeqiNP_566824.1. NM_113690.3. [Q9LVW7-1]
UniGeneiAt.8030.

Genome annotation databases

EnsemblPlantsiAT3G27740.1; AT3G27740.1; AT3G27740. [Q9LVW7-1]
GeneIDi822396.
GrameneiAT3G27740.1; AT3G27740.1; AT3G27740.
KEGGiath:AT3G27740.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018114 Genomic DNA. Translation: BAB02698.1.
CP002686 Genomic DNA. Translation: AEE77358.1.
AY046004 mRNA. Translation: AAK76678.1.
AY079315 mRNA. Translation: AAL85046.1.
U73175 mRNA. Translation: AAC25961.1. Different initiation.
RefSeqiNP_566824.1. NM_113690.3. [Q9LVW7-1]
UniGeneiAt.8030.

3D structure databases

ProteinModelPortaliQ9LVW7.
SMRiQ9LVW7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7726. 2 interactors.
IntActiQ9LVW7. 1 interactor.
STRINGi3702.AT3G27740.1.

Protein family/group databases

MEROPSiC26.A04.

Proteomic databases

PaxDbiQ9LVW7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27740.1; AT3G27740.1; AT3G27740. [Q9LVW7-1]
GeneIDi822396.
GrameneiAT3G27740.1; AT3G27740.1; AT3G27740.
KEGGiath:AT3G27740.

Organism-specific databases

AraportiAT3G27740.
TAIRilocus:2089149. AT3G27740.

Phylogenomic databases

eggNOGiENOG410IUMV. Eukaryota.
COG0505. LUCA.
HOGENOMiHOG000038087.
InParanoidiQ9LVW7.
KOiK01956.
OMAiCFSVQYH.
OrthoDBiEOG093609NY.
PhylomeDBiQ9LVW7.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.
BioCyciARA:AT3G27740-MONOMER.

Miscellaneous databases

PROiPR:Q9LVW7.

Gene expression databases

ExpressionAtlasiQ9LVW7. baseline and differential.
GenevisibleiQ9LVW7. AT.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_ARATH
AccessioniPrimary (citable) accession number: Q9LVW7
Secondary accession number(s): O24447
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: October 1, 2000
Last modified: April 12, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.