Q9LVQ8 (P2C80_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 80
Short name=AtPP2C80
EC=3.1.3.16

Gene names

Ordered Locus Names:	At5g66720
ORF Names:	MSN2.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence BX833253 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 18633119. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9LVQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9LVQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     167-169: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Probable protein phosphatase 2C 80		PRO_0000367999
Regions
	Domain	170 – 411	242	PP2C-like
Sites
	Metal binding	204	1	Manganese 1 By similarity
	Metal binding	204	1	Manganese 2 By similarity
	Metal binding	205	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	336	1	Manganese 2 By similarity
	Metal binding	402	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	167 – 169	3	Missing in isoform 2.		VSP_036777

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 795E4D79381D5789	FASTA	414	43,873
	10 20 30 40 50 60 MSATALSRLN PVSQFGFQRI VAGKSKSFFS NSGQRRLFSD SSRFRQAMAA SGSLPVFGDA 70 80 90 100 110 120 CLDDLVTTCS NGLDFTKKRS SGGSFTINCP VASMRLGKRG GMMKNRLVCH YSVVDPLEKS 130 140 150 160 170 180 RALFGTLSKS VHTSPMACFS VGPAHELSSL NGGSQESPPT TTTSLKSLRL VSGSCYLPHP 190 200 210 220 230 240 EKEATGGEDA HFICDEEQAI GVADGVGGWA EVGVNAGLFS RELMSYSVSA IQEQHKGSSI 250 260 270 280 290 300 DPLVVLEKAH SQTKAKGSST ACIIVLKDKG LHAINLGDSG FTVVREGTTV FQSPVQQHGF 310 320 330 340 350 360 NFTYQLESGN SADVPSSGQV FTIDVQSGDV IVAGTDGVYD NLYNEEITGV VVSSVRAGLD 370 380 390 400 410 PKGTAQKIAE LARQRAVDKK RQSPFATAAQ EAGYRYYGGK LDDITAVVSY VTSS
	Isoform 2 [UniParc]. Checksum: FF894EE7D74F49E3	FASTA	411	43,517
	10 20 30 40 50 60 MSATALSRLN PVSQFGFQRI VAGKSKSFFS NSGQRRLFSD SSRFRQAMAA SGSLPVFGDA 70 80 90 100 110 120 CLDDLVTTCS NGLDFTKKRS SGGSFTINCP VASMRLGKRG GMMKNRLVCH YSVVDPLEKS 130 140 150 160 170 180 RALFGTLSKS VHTSPMACFS VGPAHELSSL NGGSQESPPT TTTSLKLVSG SCYLPHPEKE 190 200 210 220 230 240 ATGGEDAHFI CDEEQAIGVA DGVGGWAEVG VNAGLFSREL MSYSVSAIQE QHKGSSIDPL 250 260 270 280 290 300 VVLEKAHSQT KAKGSSTACI IVLKDKGLHA INLGDSGFTV VREGTTVFQS PVQQHGFNFT 310 320 330 340 350 360 YQLESGNSAD VPSSGQVFTI DVQSGDVIVA GTDGVYDNLY NEEITGVVVS SVRAGLDPKG 370 380 390 400 410 TAQKIAELAR QRAVDKKRQS PFATAAQEAG YRYYGGKLDD ITAVVSYVTS S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[6]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018119 Genomic DNA. Translation: BAA97278.1. CP002688 Genomic DNA. Translation: AED98255.1. CP002688 Genomic DNA. Translation: AED98256.1. AK117127 mRNA. Translation: BAC41805.1. AY136405 mRNA. Translation: AAM97071.1. BT000228 mRNA. Translation: AAN15547.1. BX833253 mRNA. No translation available.
IPI	IPI00527504. IPI00542549.
RefSeq	NP_201473.1. NM_126070.3. NP_975004.1. NM_203275.1.
UniGene	At.28853.
3D structure databases
ProteinModelPortal	Q9LVQ8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LVQ8. 12 interactions.
Proteomic databases
PaxDb	Q9LVQ8.
PRIDE	Q9LVQ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G66720.1; AT5G66720.1; AT5G66720.
GeneID	836805.
KEGG	ath:AT5G66720.
Organism-specific databases
TAIR	At5g66720.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000239282.
InParanoid	Q9LVQ8.
OMA	TVFQSPV.
PhylomeDB	Q9LVQ8.
ProtClustDB	CLSN2686876.
Gene expression databases
Genevestigator	Q9LVQ8.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C80_ARATH

Accession

Primary (citable) accession number: Q9LVQ8
Secondary accession number(s): Q3E849

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program