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Protein

Pectinesterase 31

Gene

PME31

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin (By similarity). Acts in a blockwise manner, resulting in a cell wall rigidification.By similarity1 Publication

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Enzyme regulationi

Does not require salt for activity. Not inhibited by kiwi pectin methylesterase inhibitor (PMEI).1 Publication

pH dependencei

Optimum pH is 8.0-8.5.1 Publication

Temperature dependencei

Fully active at 0 or 30 degrees Celsius, inactive at 55 degrees Celsius.1 Publication

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (At3g14310), Pectinesterase/pectinesterase inhibitor 18 (PME18), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Putative pectinesterase 10 (PME10), Pectinesterase (F14I3.7), Pectinesterase 2 (PME2), Probable pectinesterase 29 (PME29), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Probable pectinesterase 8 (PME8), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase QRT1 (QRT1), Putative pectinesterase 11 (PME11), Pectinesterase PPME1 (PPME1), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_AT1G48300), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase, Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Probable pectinesterase/pectinesterase inhibitor 6 (PME6)
  2. Pectate lyase (T26I12.20), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 6 (At2g02720), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase (At3g07010), Pectate lyase, Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (At5g04310), Pectate lyase (At4g13710), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Pectate lyase (At3g01270), Putative pectate lyase 11 (At3g27400), Pectate lyase (At5g04310), Pectate lyase (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei91 – 911SubstrateBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Sitei143 – 1431Transition state stabilizerBy similarity
Active sitei144 – 1441Proton donorPROSITE-ProRule annotation
Active sitei165 – 1651NucleophilePROSITE-ProRule annotation
Binding sitei222 – 2221SubstrateBy similarity
Binding sitei224 – 2241SubstrateBy similarity

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: TAIR

GO - Biological processi

  • cell wall modification Source: InterPro
  • defense response to Gram-negative bacterium Source: TAIR
  • pectin catabolic process Source: UniProtKB-UniPathway
  • pectin metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G29090-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase 31 (EC:3.1.1.11)
Short name:
PE 31
Alternative name(s):
Pectin methylesterase 31
Short name:
AtPME31
Gene namesi
Name:PME31
Synonyms:ARATH31
Ordered Locus Names:At3g29090
ORF Names:MXE2.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G29090.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Pectinesterase 31PRO_0000371683Add
BLAST

Proteomic databases

PaxDbiQ9LVQ0.
PRIDEiQ9LVQ0.

Expressioni

Tissue specificityi

Expressed in siliques.1 Publication

Developmental stagei

Expressed during late developmental phases of siliques.1 Publication

Gene expression databases

GenevisibleiQ9LVQ0. AT.

Interactioni

Protein-protein interaction databases

BioGridi7885. 3 interactions.
IntActiQ9LVQ0. 2 interactions.
STRINGi3702.AT3G29090.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LVQ0.
SMRiQ9LVQ0. Positions 7-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Phylogenomic databases

eggNOGiENOG410IKYQ. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ9LVQ0.
KOiK01051.
OMAiNRVAWCR.
OrthoDBiEOG09360FJO.
PhylomeDBiQ9LVQ0.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LVQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTRMVRVS QDGSGDYCSV QDAIDSVPLG NTCRTVIRLS PGIYRQPVYV
60 70 80 90 100
PKRKNFITFA GISPEITVLT WNNTASKIEH HQASRVIGTG TFGCGSVIVE
110 120 130 140 150
GEDFIAENIT FENSAPEGSG QAVAIRVTAD RCAFYNCRFL GWQDTLYLHH
160 170 180 190 200
GKQYLKDCYI EGSVDFIFGN STALLEHCHI HCKSQGFITA QSRKSSQEST
210 220 230 240 250
GYVFLRCVIT GNGQSGYMYL GRPWGPFGRV VLAYTYMDAC IRNVGWHNWG
260 270 280 290 300
NAENERSACF YEYRCFGPGS CSSERVPWSR ELMDDEAGHF VHHSFVDPEQ
310
DRPWLCLRMG VKTPYSA
Length:317
Mass (Da):35,520
Last modified:October 1, 2000 - v1
Checksum:iBCD96098853B585B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018121 Genomic DNA. Translation: BAB01985.1.
CP002686 Genomic DNA. Translation: AEE77534.1.
AY070091 mRNA. Translation: AAL49785.1.
AY096561 mRNA. Translation: AAM20211.1.
AY084418 mRNA. Translation: AAM60992.1.
RefSeqiNP_566842.1. NM_113832.3.
UniGeneiAt.28398.

Genome annotation databases

EnsemblPlantsiAT3G29090.1; AT3G29090.1; AT3G29090.
GeneIDi822556.
GrameneiAT3G29090.1; AT3G29090.1; AT3G29090.
KEGGiath:AT3G29090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018121 Genomic DNA. Translation: BAB01985.1.
CP002686 Genomic DNA. Translation: AEE77534.1.
AY070091 mRNA. Translation: AAL49785.1.
AY096561 mRNA. Translation: AAM20211.1.
AY084418 mRNA. Translation: AAM60992.1.
RefSeqiNP_566842.1. NM_113832.3.
UniGeneiAt.28398.

3D structure databases

ProteinModelPortaliQ9LVQ0.
SMRiQ9LVQ0. Positions 7-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7885. 3 interactions.
IntActiQ9LVQ0. 2 interactions.
STRINGi3702.AT3G29090.1.

Proteomic databases

PaxDbiQ9LVQ0.
PRIDEiQ9LVQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G29090.1; AT3G29090.1; AT3G29090.
GeneIDi822556.
GrameneiAT3G29090.1; AT3G29090.1; AT3G29090.
KEGGiath:AT3G29090.

Organism-specific databases

TAIRiAT3G29090.

Phylogenomic databases

eggNOGiENOG410IKYQ. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiQ9LVQ0.
KOiK01051.
OMAiNRVAWCR.
OrthoDBiEOG09360FJO.
PhylomeDBiQ9LVQ0.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT3G29090-MONOMER.

Miscellaneous databases

PROiQ9LVQ0.

Gene expression databases

GenevisibleiQ9LVQ0. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME31_ARATH
AccessioniPrimary (citable) accession number: Q9LVQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This is the only member of the pectinesterase family that do not contain a transmembrane or a signal peptide.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.