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Protein

Ubiquitin-like protein ATG12B

Gene

ATG12B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. ATG12/ATG5 conjugate has an essential role in plant nutrient recycling.1 Publication

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagy Source: TAIR
  • autophagy of mitochondrion Source: GO_Central
  • autophagy of nucleus Source: GO_Central
  • C-terminal protein lipidation Source: GO_Central
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processAutophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein ATG12B
Alternative name(s):
Autophagy-related protein 12b
Short name:
APG12-like protein b
Short name:
AtAPG12b
Gene namesi
Name:ATG12B
Synonyms:APG12, APG12B
Ordered Locus Names:At3g13970
ORF Names:MDC16.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G13970
TAIRilocus:2088182 AT3G13970

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002505432 – 94Ubiquitin-like protein ATG12BAdd BLAST93

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki94Glycyl lysine isopeptide (Gly-Lys) (interchain with K-128 in ATG5)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

PaxDbiQ9LVK3

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ9LVK3 baseline and differential

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG3Q0WWQ12EBI-8276588,EBI-8276607

Protein-protein interaction databases

BioGridi5945, 1 interactor
IntActiQ9LVK3, 1 interactor
MINTiQ9LVK3
STRINGi3702.AT3G13970.1

Structurei

Secondary structure

194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 18Combined sources8
Beta strandi29 – 33Combined sources5
Helixi39 – 49Combined sources11
Beta strandi55 – 62Combined sources8
Helixi70 – 77Combined sources8
Beta strandi82 – 89Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WZ3X-ray1.80A/B1-94[»]
ProteinModelPortaliQ9LVK3
SMRiQ9LVK3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9LVK3

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG12 family.Curated

Phylogenomic databases

eggNOGiKOG3439 Eukaryota
ENOG4111ND7 LUCA
HOGENOMiHOG000234863
InParanoidiQ9LVK3
KOiK08336
OMAiERTPESG
OrthoDBiEOG09360XC2
PhylomeDBiQ9LVK3

Family and domain databases

CDDicd01612 APG12_C, 1 hit
InterProiView protein in InterPro
IPR007242 Atg12
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR13385 PTHR13385, 1 hit
PfamiView protein in Pfam
PF04110 APG12, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LVK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATESPNSVQ KIVVHLRATG GAPILKQSKF KVSGSDKFAN VIDFLRRQLH
60 70 80 90
SDSLFVYVNS AFSPNPDESV IDLYNNFGFD GKLVVNYACS MAWG
Length:94
Mass (Da):10,358
Last modified:October 1, 2000 - v1
Checksum:i5BA77795F9C89D6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB073185 mRNA Translation: BAB88397.1
AB019229 Genomic DNA Translation: BAB02327.1
CP002686 Genomic DNA Translation: AEE75446.1
BT024653 mRNA Translation: ABD57478.1
AK118997 mRNA Translation: BAC43573.1
RefSeqiNP_188013.2, NM_112251.4
UniGeneiAt.39312

Genome annotation databases

EnsemblPlantsiAT3G13970.1; AT3G13970.1; AT3G13970
GeneIDi820611
GrameneiAT3G13970.1; AT3G13970.1; AT3G13970
KEGGiath:AT3G13970

Similar proteinsi

Entry informationi

Entry nameiAT12B_ARATH
AccessioniPrimary (citable) accession number: Q9LVK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health