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Protein

Glutamine synthetase cytosolic isozyme 1-3

Gene

GLN1-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=3.9 mM for glutamate1 Publication
  2. KM=1210 µM for ammonium1 Publication
  3. KM=850 µM for ATP1 Publication
  1. Vmax=162 nmol/sec/mg enzyme with glutamate as substrate1 Publication
  2. Vmax=93.9 nmol/sec/mg enzyme with ammonium as substrate1 Publication
  3. Vmax=100 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  • glutamine biosynthetic process Source: InterPro
  • nitrate assimilation Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiR-ATH-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-ATH-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKQ9LVI8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-3 (EC:6.3.1.2)
Short name:
GS1
Alternative name(s):
Glutamate--ammonia ligase GLN1;3
Short name:
GLN1;3
Gene namesi
Name:GLN1-3
Ordered Locus Names:At3g17820
ORF Names:MEB5.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G17820.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49K → Q: 3-fold increase in affinity for ammonium and catalytic efficiency. 1 Publication1
Mutagenesisi174A → S: 4-fold increase in affinity for ammonium and catalytic efficiency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001531682 – 354Glutamine synthetase cytosolic isozyme 1-3Add BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineBy similarity1
Modified residuei48PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9LVI8.
PRIDEiQ9LVI8.

PTM databases

iPTMnetiQ9LVI8.

Expressioni

Tissue specificityi

Expressed in the pericycle in the region of mature root.1 Publication

Inductioni

By sucrose, glucose and fructose. Down-regulated by ammonium supply.2 Publications

Gene expression databases

GenevisibleiQ9LVI8. AT.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

BioGridi6383. 2 interactors.
STRINGi3702.AT3G17820.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LVI8.
SMRiQ9LVI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
HOGENOMiHOG000061500.
InParanoidiQ9LVI8.
KOiK01915.
OMAiKTMRNDG.
OrthoDBiEOG09360CB0.
PhylomeDBiQ9LVI8.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LVI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSDLVNL NLTDATGKII AEYIWIGGSG MDIRSKARTL PGPVTDPSKL
60 70 80 90 100
PKWNYDGSST GQAAGEDSEV ILYPQAIFKD PFRKGNNILV MCDAYTPAGD
110 120 130 140 150
PIPTNKRHNA AKIFSHPDVA KEEPWYGIEQ EYTLMQKDVN WPIGWPVGGY
160 170 180 190 200
PGPQGPYYCG VGADKAIGRD IVDAHYKACL YAGIGISGIN GEVMPGQWEF
210 220 230 240 250
QVGPVEGISS GDQVWVARYL LERITEISGV IVSFDPKPVP GDWNGAGAHC
260 270 280 290 300
NYSTKTMRND GGLEVIKKAI GKLQLKHKEH IAAYGEGNER RLTGKHETAD
310 320 330 340 350
INTFSWGVAN RGASVRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIAET

TILG
Length:354
Mass (Da):38,595
Last modified:October 1, 2000 - v1
Checksum:i750441568FA1D7B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85G → D in AAO42253 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019230 Genomic DNA. Translation: BAB02705.1.
CP002686 Genomic DNA. Translation: AEE76011.1.
BT004249 mRNA. Translation: AAO42253.1.
AY088312 mRNA. Translation: AAM65851.1.
BT020327 mRNA. Translation: AAV85682.1.
BT020432 mRNA. Translation: AAW28559.1.
RefSeqiNP_188409.1. NM_112663.3.
UniGeneiAt.26821.

Genome annotation databases

EnsemblPlantsiAT3G17820.1; AT3G17820.1; AT3G17820.
GeneIDi821050.
GrameneiAT3G17820.1; AT3G17820.1; AT3G17820.
KEGGiath:AT3G17820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019230 Genomic DNA. Translation: BAB02705.1.
CP002686 Genomic DNA. Translation: AEE76011.1.
BT004249 mRNA. Translation: AAO42253.1.
AY088312 mRNA. Translation: AAM65851.1.
BT020327 mRNA. Translation: AAV85682.1.
BT020432 mRNA. Translation: AAW28559.1.
RefSeqiNP_188409.1. NM_112663.3.
UniGeneiAt.26821.

3D structure databases

ProteinModelPortaliQ9LVI8.
SMRiQ9LVI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6383. 2 interactors.
STRINGi3702.AT3G17820.1.

PTM databases

iPTMnetiQ9LVI8.

Proteomic databases

PaxDbiQ9LVI8.
PRIDEiQ9LVI8.

Protocols and materials databases

DNASUi821050.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17820.1; AT3G17820.1; AT3G17820.
GeneIDi821050.
GrameneiAT3G17820.1; AT3G17820.1; AT3G17820.
KEGGiath:AT3G17820.

Organism-specific databases

TAIRiAT3G17820.

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
HOGENOMiHOG000061500.
InParanoidiQ9LVI8.
KOiK01915.
OMAiKTMRNDG.
OrthoDBiEOG09360CB0.
PhylomeDBiQ9LVI8.

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiR-ATH-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-ATH-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKQ9LVI8.

Miscellaneous databases

PROiQ9LVI8.

Gene expression databases

GenevisibleiQ9LVI8. AT.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLN13_ARATH
AccessioniPrimary (citable) accession number: Q9LVI8
Secondary accession number(s): Q5PNX0, Q84W44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.