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Q9LVI8

- GLN13_ARATH

UniProt

Q9LVI8 - GLN13_ARATH

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Protein

Glutamine synthetase cytosolic isozyme 1-3

Gene

GLN1-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Low-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=3.9 mM for glutamate1 Publication
  2. KM=1210 µM for ammonium1 Publication
  3. KM=850 µM for ATP1 Publication

Vmax=162 nmol/sec/mg enzyme with glutamate as substrate1 Publication

Vmax=93.9 nmol/sec/mg enzyme with ammonium as substrate1 Publication

Vmax=100 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. copper ion binding Source: TAIR
  3. glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrate assimilation Source: TAIR
  3. response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiREACT_232680. Amino acid synthesis and interconversion (transamination).
REACT_262794. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKQ9LVI8.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-3 (EC:6.3.1.2)
Short name:
GS1
Alternative name(s):
Glutamate--ammonia ligase GLN1;3
Short name:
GLN1;3
Gene namesi
Name:GLN1-3
Ordered Locus Names:At3g17820
ORF Names:MEB5.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G17820.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. cytosol Source: TAIR
  3. cytosolic ribosome Source: TAIR
  4. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491K → Q: 3-fold increase in affinity for ammonium and catalytic efficiency. 1 Publication
Mutagenesisi174 – 1741A → S: 4-fold increase in affinity for ammonium and catalytic efficiency. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 354353Glutamine synthetase cytosolic isozyme 1-3PRO_0000153168Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9LVI8.
PRIDEiQ9LVI8.

Expressioni

Tissue specificityi

Expressed in the pericycle in the region of mature root.1 Publication

Inductioni

By sucrose, glucose and fructose. Down-regulated by ammonium supply.2 Publications

Gene expression databases

ExpressionAtlasiQ9LVI8. baseline and differential.
GenevestigatoriQ9LVI8.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

BioGridi6383. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9LVI8.
SMRiQ9LVI8. Positions 4-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ9LVI8.
KOiK01915.
OMAiDVEYHCK.
PhylomeDBiQ9LVI8.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LVI8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLLSDLVNL NLTDATGKII AEYIWIGGSG MDIRSKARTL PGPVTDPSKL
60 70 80 90 100
PKWNYDGSST GQAAGEDSEV ILYPQAIFKD PFRKGNNILV MCDAYTPAGD
110 120 130 140 150
PIPTNKRHNA AKIFSHPDVA KEEPWYGIEQ EYTLMQKDVN WPIGWPVGGY
160 170 180 190 200
PGPQGPYYCG VGADKAIGRD IVDAHYKACL YAGIGISGIN GEVMPGQWEF
210 220 230 240 250
QVGPVEGISS GDQVWVARYL LERITEISGV IVSFDPKPVP GDWNGAGAHC
260 270 280 290 300
NYSTKTMRND GGLEVIKKAI GKLQLKHKEH IAAYGEGNER RLTGKHETAD
310 320 330 340 350
INTFSWGVAN RGASVRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIAET

TILG
Length:354
Mass (Da):38,595
Last modified:October 1, 2000 - v1
Checksum:i750441568FA1D7B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti85 – 851G → D in AAO42253. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019230 Genomic DNA. Translation: BAB02705.1.
CP002686 Genomic DNA. Translation: AEE76011.1.
BT004249 mRNA. Translation: AAO42253.1.
AY088312 mRNA. Translation: AAM65851.1.
BT020327 mRNA. Translation: AAV85682.1.
BT020432 mRNA. Translation: AAW28559.1.
RefSeqiNP_188409.1. NM_112663.2.
UniGeneiAt.26821.

Genome annotation databases

EnsemblPlantsiAT3G17820.1; AT3G17820.1; AT3G17820.
GeneIDi821050.
KEGGiath:AT3G17820.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019230 Genomic DNA. Translation: BAB02705.1 .
CP002686 Genomic DNA. Translation: AEE76011.1 .
BT004249 mRNA. Translation: AAO42253.1 .
AY088312 mRNA. Translation: AAM65851.1 .
BT020327 mRNA. Translation: AAV85682.1 .
BT020432 mRNA. Translation: AAW28559.1 .
RefSeqi NP_188409.1. NM_112663.2.
UniGenei At.26821.

3D structure databases

ProteinModelPortali Q9LVI8.
SMRi Q9LVI8. Positions 4-353.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 6383. 1 interaction.

Proteomic databases

PaxDbi Q9LVI8.
PRIDEi Q9LVI8.

Protocols and materials databases

DNASUi 821050.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G17820.1 ; AT3G17820.1 ; AT3G17820 .
GeneIDi 821050.
KEGGi ath:AT3G17820.

Organism-specific databases

TAIRi AT3G17820.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi Q9LVI8.
KOi K01915.
OMAi DVEYHCK.
PhylomeDBi Q9LVI8.

Enzyme and pathway databases

BRENDAi 6.3.1.2. 399.
Reactomei REACT_232680. Amino acid synthesis and interconversion (transamination).
REACT_262794. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RK Q9LVI8.

Gene expression databases

ExpressionAtlasi Q9LVI8. baseline and differential.
Genevestigatori Q9LVI8.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
    Oliveira I.C., Coruzzi G.M.
    Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
    Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
    J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
  8. "Gln49 and Ser174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase."
    Ishiyama K., Inoue E., Yamaya T., Takahashi H.
    Plant Cell Physiol. 47:299-303(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-49 AND ALA-174.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLN13_ARATH
AccessioniPrimary (citable) accession number: Q9LVI8
Secondary accession number(s): Q5PNX0, Q84W44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3