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Q9LVI8

- GLN13_ARATH

UniProt

Q9LVI8 - GLN13_ARATH

Protein

Glutamine synthetase cytosolic isozyme 1-3

Gene

GLN1-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Low-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Kineticsi

    Measured at pH 7.8 and 30 degrees Celsius for all experiments.

    1. KM=3.9 mM for glutamate1 Publication
    2. KM=1210 µM for ammonium1 Publication
    3. KM=850 µM for ATP1 Publication

    Vmax=162 nmol/sec/mg enzyme with glutamate as substrate1 Publication

    Vmax=93.9 nmol/sec/mg enzyme with ammonium as substrate1 Publication

    Vmax=100 nmol/sec/mg enzyme with ATP as substrate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. copper ion binding Source: TAIR
    3. glutamate-ammonia ligase activity Source: TAIR

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrate assimilation Source: TAIR
    3. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.3.1.2. 399.
    SABIO-RKQ9LVI8.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-3 (EC:6.3.1.2)
    Short name:
    GS1
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;3
    Short name:
    GLN1;3
    Gene namesi
    Name:GLN1-3
    Ordered Locus Names:At3g17820
    ORF Names:MEB5.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G17820.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. cytosol Source: TAIR
    3. cytosolic ribosome Source: TAIR
    4. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi49 – 491K → Q: 3-fold increase in affinity for ammonium and catalytic efficiency. 1 Publication
    Mutagenesisi174 – 1741A → S: 4-fold increase in affinity for ammonium and catalytic efficiency. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 354353Glutamine synthetase cytosolic isozyme 1-3PRO_0000153168Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei48 – 481PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9LVI8.
    PRIDEiQ9LVI8.

    Expressioni

    Tissue specificityi

    Expressed in the pericycle in the region of mature root.1 Publication

    Inductioni

    By sucrose, glucose and fructose. Down-regulated by ammonium supply.2 Publications

    Gene expression databases

    ArrayExpressiQ9LVI8.
    GenevestigatoriQ9LVI8.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    BioGridi6383. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LVI8.
    SMRiQ9LVI8. Positions 4-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    InParanoidiQ9LVI8.
    KOiK01915.
    OMAiDVEYHCK.
    PhylomeDBiQ9LVI8.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LVI8-1 [UniParc]FASTAAdd to Basket

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    MSLLSDLVNL NLTDATGKII AEYIWIGGSG MDIRSKARTL PGPVTDPSKL    50
    PKWNYDGSST GQAAGEDSEV ILYPQAIFKD PFRKGNNILV MCDAYTPAGD 100
    PIPTNKRHNA AKIFSHPDVA KEEPWYGIEQ EYTLMQKDVN WPIGWPVGGY 150
    PGPQGPYYCG VGADKAIGRD IVDAHYKACL YAGIGISGIN GEVMPGQWEF 200
    QVGPVEGISS GDQVWVARYL LERITEISGV IVSFDPKPVP GDWNGAGAHC 250
    NYSTKTMRND GGLEVIKKAI GKLQLKHKEH IAAYGEGNER RLTGKHETAD 300
    INTFSWGVAN RGASVRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIAET 350
    TILG 354
    Length:354
    Mass (Da):38,595
    Last modified:October 1, 2000 - v1
    Checksum:i750441568FA1D7B9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851G → D in AAO42253. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019230 Genomic DNA. Translation: BAB02705.1.
    CP002686 Genomic DNA. Translation: AEE76011.1.
    BT004249 mRNA. Translation: AAO42253.1.
    AY088312 mRNA. Translation: AAM65851.1.
    BT020327 mRNA. Translation: AAV85682.1.
    BT020432 mRNA. Translation: AAW28559.1.
    RefSeqiNP_188409.1. NM_112663.2.
    UniGeneiAt.26821.

    Genome annotation databases

    EnsemblPlantsiAT3G17820.1; AT3G17820.1; AT3G17820.
    GeneIDi821050.
    KEGGiath:AT3G17820.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019230 Genomic DNA. Translation: BAB02705.1 .
    CP002686 Genomic DNA. Translation: AEE76011.1 .
    BT004249 mRNA. Translation: AAO42253.1 .
    AY088312 mRNA. Translation: AAM65851.1 .
    BT020327 mRNA. Translation: AAV85682.1 .
    BT020432 mRNA. Translation: AAW28559.1 .
    RefSeqi NP_188409.1. NM_112663.2.
    UniGenei At.26821.

    3D structure databases

    ProteinModelPortali Q9LVI8.
    SMRi Q9LVI8. Positions 4-353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 6383. 1 interaction.

    Proteomic databases

    PaxDbi Q9LVI8.
    PRIDEi Q9LVI8.

    Protocols and materials databases

    DNASUi 821050.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G17820.1 ; AT3G17820.1 ; AT3G17820 .
    GeneIDi 821050.
    KEGGi ath:AT3G17820.

    Organism-specific databases

    TAIRi AT3G17820.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    InParanoidi Q9LVI8.
    KOi K01915.
    OMAi DVEYHCK.
    PhylomeDBi Q9LVI8.

    Enzyme and pathway databases

    BRENDAi 6.3.1.2. 399.
    SABIO-RK Q9LVI8.

    Gene expression databases

    ArrayExpressi Q9LVI8.
    Genevestigatori Q9LVI8.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Arabidopsis ORF clones."
      Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
      Oliveira I.C., Coruzzi G.M.
      Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
      Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
      J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    8. "Gln49 and Ser174 residues play critical roles in determining the catalytic efficiencies of plant glutamine synthetase."
      Ishiyama K., Inoue E., Yamaya T., Takahashi H.
      Plant Cell Physiol. 47:299-303(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LYS-49 AND ALA-174.
    9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLN13_ARATH
    AccessioniPrimary (citable) accession number: Q9LVI8
    Secondary accession number(s): Q5PNX0, Q84W44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3