Reviewed, UniProtKB/Swiss-Prot Q9LVI4 (TIF6B_ARATH)

Names and origin

Protein names

Recommended name:
    Protein TIFY 6B
Alternative name(s):
    Jasmonate ZIM domain-containing protein 3
    Protein JASMONATE INSENSITIVE 3

Gene names

Name:	TIFY6B
Synonyms:	JAI3, JAZ3
Ordered Locus Names:	At3g17860
ORF Names:	MEB5.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Repressor of jasmonate responses negatively regulated by the proteasome in an SCF(COI1) E3 ubiquitin-protein ligase complex-dependent manner. Acts as a negative regulator of MYC2 function. Feed-back regulated by MYC2. Ref.5 Ref.6
Subunit structure	Interacts with COI1 and MYC2. Ref.6
Subcellular location	Nucleus Probable.
Tissue specificity	Srtongly expressed in root tips. Ref.7
Induction	By jasmonate. Ref.5
Domain	The C-terminal domain (298-352) is required for TIFY 6B/JAI3 instability in response to jasmonate and for the interaction with MYC2. The N-terminal part of the protein (1-208) is required for the interaction with COI1.
Post-translational modification	Ubiquitinated Probable.
Sequence similarities	Contains 1 tify domain.

Ontologies

Keywords
Biological process	Plant defense Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW jasmonic acid mediated signaling pathway Ref.6 Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
COI1	O04197	1	EBI-1792431,EBI-401159
RAP1	Q39204	1	EBI-1792431,EBI-1792336

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LVI4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Protein TIFY 6B		PRO_0000300648
Regions
	Domain	172 – 207	36	Tify
	Compositional bias	133 – 137	5	Poly-Asn
Experimental info
	Mutagenesis	299 – 312	14	VALPL…SLARF → GKKQSQRPDTTFAI in jai3-1; dominant mutation that confers jasmonate insensitivity. Ref.6
	Mutagenesis	313 – 352	40	Missing in jai3-1; dominant mutation that confers jasmonate insensitivity.
	Sequence conflict	22	1	S → R in AAM67301. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D65DEEA2F04C58E4	FASTA	352	37,746
	10 20 30 40 50 60 MERDFLGLGS KNSPITVKEE TSESSRDSAP NRGMNWSFSN KVSASSSQFL SFRPTQEDRH 70 80 90 100 110 120 RKSGNYHLPH SGSFMPSSVA DVYDSTRKAP YSSVQGVRMF PNSNQHEETN AVSMSMPGFQ 130 140 150 160 170 180 SHHYAPGGRS FMNNNNNSQP LVGVPIMAPP ISILPPPGSI VGTTDIRSSS KPIGSPAQLT 190 200 210 220 230 240 IFYAGSVCVY DDISPEKAKA IMLLAGNGSS MPQVFSPPQT HQQVVHHTRA SVDSSAMPPS 250 260 270 280 290 300 FMPTISYLSP EAGSSTNGLG ATKATRGLTS TYHNNQANGS NINCPVPVSC STNVMAPTVA 310 320 330 340 350 LPLARKASLA RFLEKRKERV TSVSPYCLDK KSSTDCRRSM SECISSSLSS AT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 30-352. Strain: cv. Columbia.
[5]	"JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling." Thines B., Katsir L., Melotto M., Niu Y., Mandaokar A., Liu G., Nomura K., He S.Y., Howe G.A., Browse J. Nature 448:661-665(2007) [PubMed: 17637677] [Abstract] Cited for: FUNCTION, INDUCTION BY JASMONATE.
[6]	"The JAZ family of repressors is the missing link in jasmonate signalling." Chini A., Fonseca S., Fernandez G., Adie B., Chico J.M., Lorenzo O., Garcia-Casado G., Lopez-Vidriero I., Lozano F.M., Ponce M.R., Micol J.L., Solano R. Nature 448:666-671(2007) [PubMed: 17637675] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF 299-VAL--THR-312, INTERACTION WITH COI1 AND MYC2, GENE FAMILY, NOMENCLATURE.
[7]	"The tify family previously known as ZIM." Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G. Trends Plant Sci. 12:239-244(2007) [PubMed: 17499004] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB019230 Genomic DNA. Translation: BAB02709.1. AY085082 mRNA. Translation: AAM67301.1. AF372907 mRNA. Translation: AAK49623.1. BT003044 mRNA. Translation: AAO23609.1. AK222169 mRNA. Translation: BAD95285.1. Different initiation.
IPI	IPI00520031.
RefSeq	NP_566590.1.
UniGene	At.8035
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LVI4. 2 interactions.
Genome annotation databases
GeneID	821055.
GenomeReviews	Gene locus AT3G17860 in contig BA000014_GR.
NMPDR	fig|3702.1.peg.13981.
Organism-specific databases
TAIR	At3g17860.
Phylogenomic databases
OMA	Q9LVI4. MSECISS.
Family and domain databases
InterPro	IPR018467. CCT_dom-like. IPR010399. Tify. [Graphical view]
Pfam	PF09425. CCT_2. 1 hit. PF06200. tify. 1 hit. [Graphical view]
PROSITE	PS51320. TIFY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIF6B_ARATH

Accession

Primary (citable) accession number: Q9LVI4
Secondary accession number(s): Q56W78, Q8LF26

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)