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Protein

Protein TIFY 6B

Gene

TIFY6B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY6B/JAZ3 interaction. Acts as a negative regulator of MYC2 function. Feed-back regulated by MYC2.4 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • transcription corepressor activity Source: GO_Central

GO - Biological processi

Keywordsi

Biological processJasmonic acid signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TIFY 6B
Alternative name(s):
Jasmonate ZIM domain-containing protein 3
Protein JASMONATE INSENSITIVE 3
Gene namesi
Name:TIFY6B
Synonyms:JAI3, JAZ3
Ordered Locus Names:At3g17860
ORF Names:MEB5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2088530. AT3G17860.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi180T → A: No effect on dimerization. 1 Publication1
Mutagenesisi181I → A: No effect on dimerization, but loss of interaction with TIFY9/JAZ10. 1 Publication1
Mutagenesisi182F → A: No effect on dimerization. 1 Publication1
Mutagenesisi183Y → A: No effect on dimerization. 1 Publication1
Mutagenesisi185G → A: Loss of dimerization and loss of interaction with TIFY9/JAZ10. 1 Publication1
Mutagenesisi299 – 312VALPL…SLARF → GKKQSQRPDTTFAI in jai3-1; dominant mutation that confers jasmonate insensitivity. 1 PublicationAdd BLAST14
Mutagenesisi313 – 352Missing in jai3-1; dominant mutation that confers jasmonate insensitivity. Add BLAST40

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003006481 – 352Protein TIFY 6BAdd BLAST352

Post-translational modificationi

Ubiquitinated. Targeted for degradation by the SCF(COI1) E3 ubiquitin ligase-proteasome pathway during jasmonate signaling.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9LVI4.

Expressioni

Tissue specificityi

Srtongly expressed in root tips.1 Publication

Inductioni

Up-regulated by jasmonate, wounding and herbivory.2 Publications

Gene expression databases

ExpressionAtlasiQ9LVI4. baseline and differential.
GenevisibleiQ9LVI4. AT.

Interactioni

Subunit structurei

Homo- and heterodimer. Interacts with COI1, MYC2, MYC3, MYC4, TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6A/JAZ4, TIFY5A/JAZ8, TIFY7/JAZ9, TIFY9/JAZ10 and TIFY3A/JAZ11. Interacts (via TIFY domain) with AFPH2/NINJA.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi6388. 43 interactors.
DIPiDIP-40532N.
IntActiQ9LVI4. 33 interactors.
STRINGi3702.AT3G17860.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LVI4.
SMRiQ9LVI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini172 – 207TifyPROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi133 – 137Poly-Asn5

Domaini

The tify domain is required for homo- and heterodimerization and for the interaction with other TIFY proteins.1 Publication
The jas domain (302-326) is necessary and sufficient for the hormone dependent interaction with COI1 and the hormone independent interaction with MYC2.1 Publication

Sequence similaritiesi

Belongs to the TIFY/JAZ family.Curated

Phylogenomic databases

eggNOGiENOG410IYU0. Eukaryota.
ENOG410XTRE. LUCA.
HOGENOMiHOG000015145.
InParanoidiQ9LVI4.
KOiK13464.
OMAiGMNWSFS.
OrthoDBiEOG09360IDV.
PhylomeDBiQ9LVI4.

Family and domain databases

InterProiView protein in InterPro
IPR018467. CCT_CS.
IPR010399. Tify_dom.
PfamiView protein in Pfam
PF09425. CCT_2. 1 hit.
PF06200. tify. 1 hit.
SMARTiView protein in SMART
SM00979. TIFY. 1 hit.
PROSITEiView protein in PROSITE
PS51320. TIFY. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LVI4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERDFLGLGS KNSPITVKEE TSESSRDSAP NRGMNWSFSN KVSASSSQFL
60 70 80 90 100
SFRPTQEDRH RKSGNYHLPH SGSFMPSSVA DVYDSTRKAP YSSVQGVRMF
110 120 130 140 150
PNSNQHEETN AVSMSMPGFQ SHHYAPGGRS FMNNNNNSQP LVGVPIMAPP
160 170 180 190 200
ISILPPPGSI VGTTDIRSSS KPIGSPAQLT IFYAGSVCVY DDISPEKAKA
210 220 230 240 250
IMLLAGNGSS MPQVFSPPQT HQQVVHHTRA SVDSSAMPPS FMPTISYLSP
260 270 280 290 300
EAGSSTNGLG ATKATRGLTS TYHNNQANGS NINCPVPVSC STNVMAPTVA
310 320 330 340 350
LPLARKASLA RFLEKRKERV TSVSPYCLDK KSSTDCRRSM SECISSSLSS

AT
Length:352
Mass (Da):37,746
Last modified:October 1, 2000 - v1
Checksum:iD65DEEA2F04C58E4
GO

Sequence cautioni

The sequence BAD95285 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22S → R in AAM67301 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019230 Genomic DNA. Translation: BAB02709.1.
CP002686 Genomic DNA. Translation: AEE76016.1.
AY085082 mRNA. Translation: AAM67301.1.
AF372907 mRNA. Translation: AAK49623.1.
BT003044 mRNA. Translation: AAO23609.1.
AK222169 mRNA. Translation: BAD95285.1. Different initiation.
RefSeqiNP_001078174.1. NM_001084705.3.
NP_001327107.1. NM_001338310.1.
NP_566590.1. NM_112667.4. [Q9LVI4-1]
UniGeneiAt.8035.

Genome annotation databases

EnsemblPlantsiAT3G17860.1; AT3G17860.1; AT3G17860. [Q9LVI4-1]
GeneIDi821055.
GrameneiAT3G17860.1; AT3G17860.1; AT3G17860.
KEGGiath:AT3G17860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019230 Genomic DNA. Translation: BAB02709.1.
CP002686 Genomic DNA. Translation: AEE76016.1.
AY085082 mRNA. Translation: AAM67301.1.
AF372907 mRNA. Translation: AAK49623.1.
BT003044 mRNA. Translation: AAO23609.1.
AK222169 mRNA. Translation: BAD95285.1. Different initiation.
RefSeqiNP_001078174.1. NM_001084705.3.
NP_001327107.1. NM_001338310.1.
NP_566590.1. NM_112667.4. [Q9LVI4-1]
UniGeneiAt.8035.

3D structure databases

ProteinModelPortaliQ9LVI4.
SMRiQ9LVI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6388. 43 interactors.
DIPiDIP-40532N.
IntActiQ9LVI4. 33 interactors.
STRINGi3702.AT3G17860.1.

Proteomic databases

PaxDbiQ9LVI4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17860.1; AT3G17860.1; AT3G17860. [Q9LVI4-1]
GeneIDi821055.
GrameneiAT3G17860.1; AT3G17860.1; AT3G17860.
KEGGiath:AT3G17860.

Organism-specific databases

AraportiAT3G17860.
TAIRilocus:2088530. AT3G17860.

Phylogenomic databases

eggNOGiENOG410IYU0. Eukaryota.
ENOG410XTRE. LUCA.
HOGENOMiHOG000015145.
InParanoidiQ9LVI4.
KOiK13464.
OMAiGMNWSFS.
OrthoDBiEOG09360IDV.
PhylomeDBiQ9LVI4.

Miscellaneous databases

PROiPR:Q9LVI4.

Gene expression databases

ExpressionAtlasiQ9LVI4. baseline and differential.
GenevisibleiQ9LVI4. AT.

Family and domain databases

InterProiView protein in InterPro
IPR018467. CCT_CS.
IPR010399. Tify_dom.
PfamiView protein in Pfam
PF09425. CCT_2. 1 hit.
PF06200. tify. 1 hit.
SMARTiView protein in SMART
SM00979. TIFY. 1 hit.
PROSITEiView protein in PROSITE
PS51320. TIFY. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIF6B_ARATH
AccessioniPrimary (citable) accession number: Q9LVI4
Secondary accession number(s): Q56W78, Q8LF26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2000
Last modified: April 12, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

PubMed:17637675 showed that COI1 interacts with the N-terminal part of the protein (1-208), but not with its C terminus.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.