Reviewed, UniProtKB/Swiss-Prot Q9LVG4 (APRR3_ARATH)

Names and origin

Protein names

Recommended name:
    Two-component response regulator-like APRR3
Alternative name(s):
    Pseudo-response regulator 3

Gene names

Name:	APRR3
Ordered Locus Names:	At5g60100
ORF Names:	MGO3.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Controls photoperiodic flowering response. Seems to be one of the component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Ref.1
Subcellular location	Nucleus Probable.
Induction	Expressed with a circadian rhythm showing a peak in the evening. Ref.4
Post-translational modification	Phosphorylated by WNK1. Ref.4
Sequence similarities	Belongs to the ARR-like family. Contains 1 CCT domain. Contains 1 response regulatory domain.
Caution	Lacks the phospho-accepting Asp (here Glu-116), present in the receiver domain, which is one of the conserved features of two-component response regulators (ARRs) family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of protein binding Inferred from direct assay. Source: TAIR regulation of circadian rhythm Ref.1 Inferred from expression pattern. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APRR1	Q9LKL2	1	EBI-1606968,EBI-618423

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Two-component response regulator-like APRR3		PRO_0000081436
Regions
	Domain	65 – 183	119	Response regulatory
	Domain	442 – 484	43	CCT
	Coiled coil	349 – 372	24	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9LVG4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 933B89129FA1974F	FASTA	495	55,286
	10 20 30 40 50 60 MCFNNIETGD EVETERQVFG SSEEDEFRVE DTARNTNNVQ ISQQQQQPLA HVVKWERYLP 70 80 90 100 110 120 VRSLKVLLVE NDDSTRHIVT ALLKNCSYEV TAVPDVLEAW RILEDEKSCI DLVLTEVDMP 130 140 150 160 170 180 VHSGTGLLSK IMSHKTLKNI PVIMMSSHDS MVLVFKCLSN GAVDFLVKPI RKNELKNLWQ 190 200 210 220 230 240 HVWRRCHSSS GSGSESGIHD KKSVKPESTQ GSENDASISD EHRNESGSSG GLSNQDGGSD 250 260 270 280 290 300 NGSGTQSSWT KRASDTKSTS PSNQFPDAPN KKGTYENGCA HVNRLKEAED QKEQIGTGSQ 310 320 330 340 350 360 TGMSMSKKAE EPGDLEKNAK YSVQALERNN DDTLNRSSGN SQVESKAPSS NREDLQSLEQ 370 380 390 400 410 420 TLKKTREDRD YKVGDRSVLR HSNLSAFSKY NNGATSAKKA PEENVESCSP HDSPIAKLLG 430 440 450 460 470 480 SSSSSDNPLK QQSSGSDRWA QREAALMKFR LKRKERCFEK KVRYHSRKKL AEQRPHVKGQ 490 FIRKRDDHKS GSEDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Circadian waves of expression of the APRR1/TOC1 family of pseudo-response regulators in Arabidopsis thaliana: insight into the plant circadian clock." Matsushika A., Makino S., Kojima M., Mizuno T. Plant Cell Physiol. 41:1002-1012(2000) [PubMed: 11100772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Genes encoding pseudo-response regulators: insight into His-to-Asp phosphorelay and circadian rhythm in Arabidopsis thaliana." Makino S., Kiba T., Imamura A., Hanaki N., Nakamura A., Suzuki T., Taniguchi M., Ueguchi C., Sugiyama T., Mizuno T. Plant Cell Physiol. 41:791-803(2000) [PubMed: 10945350] [Abstract] Cited for: GENE FAMILY.
[4]	"The APRR3 component of the clock-associated APRR1/TOC1 quintet is phosphorylated by a novel protein kinase belonging to the WNK family, the gene for which is also transcribed rhythmically in Arabidopsis thaliana." Murakami-Kojima M., Nakamichi N., Yamashino T., Mizuno T. Plant Cell Physiol. 43:675-683(2002) [PubMed: 12091722] [Abstract] Cited for: INDUCTION, PHOSPHORYLATION BY WNK1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB046956 mRNA. Translation: BAB13744.1. AB019231 Genomic DNA. Translation: BAA96939.1.
IPI	IPI00525359.
RefSeq	NP_568919.1.
UniGene	At.1676
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LVG4. 1 interaction.
Proteomic databases
PRIDE	Q9LVG4.
Genome annotation databases
GeneID	836132.
GenomeReviews	Gene locus AT5G60100 in contig BA000015_GR.
KEGG	ath:AT5G60100.
NMPDR	fig|3702.1.peg.28015.
Organism-specific databases
GeneFarm	4342. 437.
TAIR	At5g60100.
Phylogenomic databases
OMA	Q9LVG4. ENDASIS.
Gene expression databases
ArrayExpress	Q9LVG4.
GermOnline	AT5G60100. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR010402. CCT_domain. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF06203. CCT. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
ProDom	PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS51017. CCT. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APRR3_ARATH

Accession

Primary (citable) accession number: Q9LVG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)