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Q9LV91 (DPE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic

EC=2.4.1.25
Alternative name(s):
Amylomaltase
Disproportionating enzyme
Short name=D-enzyme
Protein DISPROPORTIONATING ENZYME 1
Gene names
Name:DPE1
Ordered Locus Names:At5g64860
ORF Names:MXK3.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Chloroplastic alpha-glucanotransferase involved in maltotriose metabolism. Probably uses maltotriose as substrate to transfer a maltosyl unit from one molecule to another, resulting in glucose and maltopentaose. The latter can then be further metabolized to maltose and maltotriose by beta-amylase. Required for normal starch degradation in leaves. Ref.4 Ref.5

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

Subcellular location

Plastidchloroplast. Plastidamyloplast Potential.

Disruption phenotype

Increased amounts of maltotriose and leaf starch. Ref.4 Ref.5

Sequence similarities

Belongs to the disproportionating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4545Chloroplast Potential
Chain46 – 5765314-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic
PRO_0000407918

Sequences

Sequence LengthMass (Da)Tools
Q9LV91 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 1363A43AF365A491

FASTA57664,412
        10         20         30         40         50         60 
MSILLRPSSS PSLCSSLKLF RLSSPDSLID AAVLRNRTKP SQSFRMEVVS SNSTCLSSIS 

        70         80         90        100        110        120 
VGEDFPSEYE QWLPVPDPES RRRAGVLLHP TSFRGPHGIG DLGEEAFRFI DWLHSTGCSV 

       130        140        150        160        170        180 
WQVLPLVPPD EGGSPYAGQD ANCGNTLLIS LDELVKDGLL IKDELPQPID ADSVNYQTAN 

       190        200        210        220        230        240 
KLKSPLITKA AKRLIDGNGE LKSKLLDFRN DPSISCWLED AAYFAAIDNT LNAYSWFEWP 

       250        260        270        280        290        300 
EPLKNRHLSA LEAIYESQKE FIDLFIAKQF LFQRQWQKVR EYARRQGVDI MGDMPIYVGY 

       310        320        330        340        350        360 
HSADVWANKK HFLLNKKGFP LLVSGVPPDL FSETGQLWGS PLYDWKAMES DQYSWWVNRI 

       370        380        390        400        410        420 
RRAQDLYDEC RIDHFRGFAG FWAVPSEAKV AMVGRWKVGP GKSLFDAISK GVGKIKIIAE 

       430        440        450        460        470        480 
DLGVITKDVV ELRKSIGAPG MAVLQFAFGG GADNPHLPHN HEVNQVVYSG THDNDTIRGW 

       490        500        510        520        530        540 
WDTLDQEEKS KAMKYLSIAG EDDISWSVIQ AAFSSTAQTA IIPMQDILGL GSSARMNTPA 

       550        560        570 
TEVGNWGWRI PSSTSFDNLE TESDRLRDLL SLYGRL 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"A critical role for disproportionating enzyme in starch breakdown is revealed by a knock-out mutation in Arabidopsis."
Critchley J.H., Zeeman S.C., Takaha T., Smith A.M., Smith S.M.
Plant J. 26:89-100(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]"Blocking the metabolism of starch breakdown products in Arabidopsis leaves triggers chloroplast degradation."
Stettler M., Eicke S., Mettler T., Messerli G., Hoertensteiner S., Zeeman S.C.
Mol. Plant 2:1233-1246(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB019236 Genomic DNA. Translation: BAA97298.1.
CP002688 Genomic DNA. Translation: AED97962.1.
AY037231 mRNA. Translation: AAK59831.1.
AY081744 mRNA. Translation: AAL87397.1.
RefSeqNP_201291.1. NM_125884.3.
UniGeneAt.7845.

3D structure databases

ProteinModelPortalQ9LV91.
SMRQ9LV91. Positions 60-576.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT5G64860.1-P.

Protein family/group databases

CAZyGH77. Glycoside Hydrolase Family 77.

Proteomic databases

PaxDbQ9LV91.
PRIDEQ9LV91.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G64860.1; AT5G64860.1; AT5G64860.
GeneID836609.
KEGGath:AT5G64860.

Organism-specific databases

TAIRAT5G64860.

Phylogenomic databases

eggNOGCOG1640.
HOGENOMHOG000017376.
InParanoidQ9LV91.
KOK00705.
OMALQFAFDG.
PhylomeDBQ9LV91.
ProtClustDBPLN02635.

Enzyme and pathway databases

BioCycARA:AT5G64860-MONOMER.
MetaCyc:AT5G64860-MONOMER.
BRENDA2.4.1.25. 399.

Gene expression databases

GenevestigatorQ9LV91.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR003385. Glyco_hydro_77.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02446. Glyco_hydro_77. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR00217. malQ. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDPE1_ARATH
AccessionPrimary (citable) accession number: Q9LV91
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names