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Q9LV91

- DPE1_ARATH

UniProt

Q9LV91 - DPE1_ARATH

Protein

4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic

Gene

DPE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Chloroplastic alpha-glucanotransferase involved in maltotriose metabolism. Probably uses maltotriose as substrate to transfer a maltosyl unit from one molecule to another, resulting in glucose and maltopentaose. The latter can then be further metabolized to maltose and maltotriose by beta-amylase. Required for normal starch degradation in leaves.2 Publications

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.

    GO - Molecular functioni

    1. 4-alpha-glucanotransferase activity Source: UniProtKB

    GO - Biological processi

    1. glucose metabolic process Source: TAIR
    2. glycogen metabolic process Source: GOC
    3. maltose catabolic process Source: UniProtKB
    4. starch catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciARA:AT5G64860-MONOMER.
    MetaCyc:AT5G64860-MONOMER.
    BRENDAi2.4.1.25. 399.

    Protein family/group databases

    CAZyiGH77. Glycoside Hydrolase Family 77.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic (EC:2.4.1.25)
    Alternative name(s):
    Amylomaltase
    Disproportionating enzyme
    Short name:
    D-enzyme
    Protein DISPROPORTIONATING ENZYME 1
    Gene namesi
    Name:DPE1
    Ordered Locus Names:At5g64860
    ORF Names:MXK3.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G64860.

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: TAIR

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Increased amounts of maltotriose and leaf starch.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545ChloroplastSequence AnalysisAdd
    BLAST
    Chaini46 – 5765314-alpha-glucanotransferase DPE1, chloroplastic/amyloplasticPRO_0000407918Add
    BLAST

    Proteomic databases

    PaxDbiQ9LV91.
    PRIDEiQ9LV91.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9LV91.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G64860.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LV91.
    SMRiQ9LV91. Positions 60-576.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the disproportionating enzyme family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1640.
    HOGENOMiHOG000017376.
    InParanoidiQ9LV91.
    KOiK00705.
    OMAiDFWEIPG.
    PhylomeDBiQ9LV91.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR003385. Glyco_hydro_77.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02446. Glyco_hydro_77. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR00217. malQ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9LV91-1 [UniParc]FASTAAdd to Basket

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    MSILLRPSSS PSLCSSLKLF RLSSPDSLID AAVLRNRTKP SQSFRMEVVS    50
    SNSTCLSSIS VGEDFPSEYE QWLPVPDPES RRRAGVLLHP TSFRGPHGIG 100
    DLGEEAFRFI DWLHSTGCSV WQVLPLVPPD EGGSPYAGQD ANCGNTLLIS 150
    LDELVKDGLL IKDELPQPID ADSVNYQTAN KLKSPLITKA AKRLIDGNGE 200
    LKSKLLDFRN DPSISCWLED AAYFAAIDNT LNAYSWFEWP EPLKNRHLSA 250
    LEAIYESQKE FIDLFIAKQF LFQRQWQKVR EYARRQGVDI MGDMPIYVGY 300
    HSADVWANKK HFLLNKKGFP LLVSGVPPDL FSETGQLWGS PLYDWKAMES 350
    DQYSWWVNRI RRAQDLYDEC RIDHFRGFAG FWAVPSEAKV AMVGRWKVGP 400
    GKSLFDAISK GVGKIKIIAE DLGVITKDVV ELRKSIGAPG MAVLQFAFGG 450
    GADNPHLPHN HEVNQVVYSG THDNDTIRGW WDTLDQEEKS KAMKYLSIAG 500
    EDDISWSVIQ AAFSSTAQTA IIPMQDILGL GSSARMNTPA TEVGNWGWRI 550
    PSSTSFDNLE TESDRLRDLL SLYGRL 576
    Length:576
    Mass (Da):64,412
    Last modified:October 1, 2000 - v1
    Checksum:i1363A43AF365A491
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019236 Genomic DNA. Translation: BAA97298.1.
    CP002688 Genomic DNA. Translation: AED97962.1.
    AY037231 mRNA. Translation: AAK59831.1.
    AY081744 mRNA. Translation: AAL87397.1.
    RefSeqiNP_201291.1. NM_125884.3.
    UniGeneiAt.7845.

    Genome annotation databases

    EnsemblPlantsiAT5G64860.1; AT5G64860.1; AT5G64860.
    GeneIDi836609.
    KEGGiath:AT5G64860.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB019236 Genomic DNA. Translation: BAA97298.1 .
    CP002688 Genomic DNA. Translation: AED97962.1 .
    AY037231 mRNA. Translation: AAK59831.1 .
    AY081744 mRNA. Translation: AAL87397.1 .
    RefSeqi NP_201291.1. NM_125884.3.
    UniGenei At.7845.

    3D structure databases

    ProteinModelPortali Q9LV91.
    SMRi Q9LV91. Positions 60-576.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G64860.1-P.

    Protein family/group databases

    CAZyi GH77. Glycoside Hydrolase Family 77.

    Proteomic databases

    PaxDbi Q9LV91.
    PRIDEi Q9LV91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G64860.1 ; AT5G64860.1 ; AT5G64860 .
    GeneIDi 836609.
    KEGGi ath:AT5G64860.

    Organism-specific databases

    TAIRi AT5G64860.

    Phylogenomic databases

    eggNOGi COG1640.
    HOGENOMi HOG000017376.
    InParanoidi Q9LV91.
    KOi K00705.
    OMAi DFWEIPG.
    PhylomeDBi Q9LV91.

    Enzyme and pathway databases

    BioCyci ARA:AT5G64860-MONOMER.
    MetaCyc:AT5G64860-MONOMER.
    BRENDAi 2.4.1.25. 399.

    Gene expression databases

    Genevestigatori Q9LV91.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR003385. Glyco_hydro_77.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02446. Glyco_hydro_77. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR00217. malQ. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "A critical role for disproportionating enzyme in starch breakdown is revealed by a knock-out mutation in Arabidopsis."
      Critchley J.H., Zeeman S.C., Takaha T., Smith A.M., Smith S.M.
      Plant J. 26:89-100(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    5. "Blocking the metabolism of starch breakdown products in Arabidopsis leaves triggers chloroplast degradation."
      Stettler M., Eicke S., Mettler T., Messerli G., Hoertensteiner S., Zeeman S.C.
      Mol. Plant 2:1233-1246(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiDPE1_ARATH
    AccessioniPrimary (citable) accession number: Q9LV91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3