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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

ASN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.1 Publication

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in the pathway L-asparagine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • ammonium ion metabolic process Source: TAIR
  • glutamine metabolic process Source: UniProtKB-KW
  • hyperosmotic salinity response Source: TAIR
  • L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  • response to absence of light Source: GO_Central

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Asparagine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-70614 Amino acid synthesis and interconversion (transamination)
UniPathwayiUPA00134

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene namesi
Name:ASN2
Ordered Locus Names:At5g65010
ORF Names:MXK3.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G65010
TAIRilocus:2177694 AT5G65010

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Pale green leaf phenotype and reduction of biomass in mature plants. Increased levels of asparagine, proline and ammonium in response to salt treatment.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004208402 – 578Asparagine synthetase [glutamine-hydrolyzing] 2Add BLAST577

Proteomic databases

PaxDbiQ9LV77
PRIDEiQ9LV77
ProMEXiQ9LV77

PTM databases

iPTMnetiQ9LV77

Expressioni

Tissue specificityi

Expressed in the vascular region adjacent to leaf mesophyll cells in the companion cell-sieve tube element complex.2 Publications

Inductioni

By light and sucrose. Down-regulated by dark.3 Publications

Gene expression databases

ExpressionAtlasiQ9LV77 baseline and differential
GenevisibleiQ9LV77 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT5G65010.2

Structurei

3D structure databases

ProteinModelPortaliQ9LV77
SMRiQ9LV77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini210 – 450Asparagine synthetaseAdd BLAST241

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi559 – 565Poly-Ala7

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571 Eukaryota
COG0367 LUCA
HOGENOMiHOG000027493
InParanoidiQ9LV77
KOiK01953
OMAiAHGESTL
PhylomeDBiQ9LV77

Family and domain databases

CDDicd01991 Asn_Synthase_B_C, 1 hit
cd00712 AsnB, 1 hit
Gene3Di3.40.50.620, 1 hit
3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR006426 Asn_synth_AEB
IPR001962 Asn_synthase
IPR033738 AsnB_N
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00733 Asn_synthase, 1 hit
PF13537 GATase_7, 1 hit
PIRSFiPIRSF001589 Asn_synthetase_glu-h, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01536 asn_synth_AEB, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LV77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGILAVLGC IDNSQAKRSR IIELSRRLRH RGPDWSGLHC YEDCYLAHER
60 70 80 90 100
LAIIDPTSGD QPLYNEDKTV AVTVNGEIYN HKILREKLKS HQFRTGSDCE
110 120 130 140 150
VIAHLYEEHG EEFIDMLDGM FAFVLLDTRD KSFIAARDAI GITPLYIGWG
160 170 180 190 200
LDGSVWFASE MKALSDDCEQ FMSFPPGHIY SSKQGGLRRW YNPPWYNEQV
210 220 230 240 250
PSTPYDPLVL RNAFEKAVIK RLMTDVPFGV LLSGGLDSSL VAAVALRHLE
260 270 280 290 300
KSEAARQWGS QLHTFCIGLQ GSPDLKAGRE VADYLGTRHH EFQFTVQDGI
310 320 330 340 350
DAIEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEILG
360 370 380 390 400
GYLYFHKAPN KKEFHEETCR KIKALHQFDC LRANKSTSAW GVEARVPFLD
410 420 430 440 450
KEFLNVAMSI DPEWKLIKPD LGRIEKWVLR NAFDDEERPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAN KHVSDTMLSN ASFVFPDNTP LTKEAYYYRT
510 520 530 540 550
IFEKFFPKSA ARATVPGGPS IACSTAKAVE WDATWSKNLD PSGRAALGVH
560 570
VAAYEEDKAA AAAKAGSDLV DPLPKNGT
Length:578
Mass (Da):65,030
Last modified:October 1, 2000 - v1
Checksum:iCAEAD8DC90F3A773
GO
Isoform 2 (identifier: Q9LV77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-508: K → KQ

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:579
Mass (Da):65,158
Checksum:iAF6CE3E95A30A9F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti484V → F in AAC72837 (PubMed:9881155).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044714508K → KQ in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095453 mRNA Translation: AAC72837.1
AB019236 Genomic DNA Translation: BAA97313.1
CP002688 Genomic DNA Translation: AED97984.1
CP002688 Genomic DNA Translation: AED97985.1
AF367340 mRNA Translation: AAK32927.1
AY124866 mRNA Translation: AAM70575.1
RefSeqiNP_201306.2, NM_125900.2 [Q9LV77-2]
NP_851272.1, NM_180941.3 [Q9LV77-1]
UniGeneiAt.67717
At.7860

Genome annotation databases

EnsemblPlantsiAT5G65010.1; AT5G65010.1; AT5G65010 [Q9LV77-1]
AT5G65010.2; AT5G65010.2; AT5G65010 [Q9LV77-2]
GeneIDi836625
GrameneiAT5G65010.1; AT5G65010.1; AT5G65010 [Q9LV77-1]
AT5G65010.2; AT5G65010.2; AT5G65010 [Q9LV77-2]
KEGGiath:AT5G65010

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASNS2_ARATH
AccessioniPrimary (citable) accession number: Q9LV77
Secondary accession number(s): F4KGG8, Q9ZST6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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