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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

ASN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for nitrogen assimilation, distribution and remobilization within the plant via the phloem.1 Publication

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway:iL-asparagine biosynthesis

This protein is involved in the pathway L-asparagine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-858-MONOMER.
ReactomeiREACT_276185. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00134.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase 2
Gene namesi
Name:ASN2
Ordered Locus Names:At5g65010
ORF Names:MXK3.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G65010.

Pathology & Biotechi

Disruption phenotypei

Pale green leaf phenotype and reduction of biomass in mature plants. Increased levels of asparagine, proline and ammonium in response to salt treatment.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 578577Asparagine synthetase [glutamine-hydrolyzing] 2PRO_0000420840Add
BLAST

Proteomic databases

PRIDEiQ9LV77.
ProMEXiQ9LV77.

Expressioni

Tissue specificityi

Expressed in the vascular region adjacent to leaf mesophyll cells in the companion cell-sieve tube element complex.2 Publications

Inductioni

By light and sucrose. Down-regulated by dark.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G65010.2.

Structurei

3D structure databases

ProteinModelPortaliQ9LV77.
SMRiQ9LV77. Positions 18-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini210 – 450241Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi559 – 5657Poly-Ala

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiQ9LV77.
KOiK01953.
OMAiSRERVYW.
PhylomeDBiQ9LV77.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LV77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCGILAVLGC IDNSQAKRSR IIELSRRLRH RGPDWSGLHC YEDCYLAHER
60 70 80 90 100
LAIIDPTSGD QPLYNEDKTV AVTVNGEIYN HKILREKLKS HQFRTGSDCE
110 120 130 140 150
VIAHLYEEHG EEFIDMLDGM FAFVLLDTRD KSFIAARDAI GITPLYIGWG
160 170 180 190 200
LDGSVWFASE MKALSDDCEQ FMSFPPGHIY SSKQGGLRRW YNPPWYNEQV
210 220 230 240 250
PSTPYDPLVL RNAFEKAVIK RLMTDVPFGV LLSGGLDSSL VAAVALRHLE
260 270 280 290 300
KSEAARQWGS QLHTFCIGLQ GSPDLKAGRE VADYLGTRHH EFQFTVQDGI
310 320 330 340 350
DAIEEVIYHI ETYDVTTIRA STPMFLMSRK IKSLGVKMVL SGEGSDEILG
360 370 380 390 400
GYLYFHKAPN KKEFHEETCR KIKALHQFDC LRANKSTSAW GVEARVPFLD
410 420 430 440 450
KEFLNVAMSI DPEWKLIKPD LGRIEKWVLR NAFDDEERPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKDHAN KHVSDTMLSN ASFVFPDNTP LTKEAYYYRT
510 520 530 540 550
IFEKFFPKSA ARATVPGGPS IACSTAKAVE WDATWSKNLD PSGRAALGVH
560 570
VAAYEEDKAA AAAKAGSDLV DPLPKNGT
Length:578
Mass (Da):65,030
Last modified:October 1, 2000 - v1
Checksum:iCAEAD8DC90F3A773
GO
Isoform 2 (identifier: Q9LV77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     508-508: K → KQ

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:579
Mass (Da):65,158
Checksum:iAF6CE3E95A30A9F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti484 – 4841V → F in AAC72837 (PubMed:9881155).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei508 – 5081K → KQ in isoform 2. CuratedVSP_044714

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095453 mRNA. Translation: AAC72837.1.
AB019236 Genomic DNA. Translation: BAA97313.1.
CP002688 Genomic DNA. Translation: AED97984.1.
CP002688 Genomic DNA. Translation: AED97985.1.
AF367340 mRNA. Translation: AAK32927.1.
AY124866 mRNA. Translation: AAM70575.1.
RefSeqiNP_201306.2. NM_125900.2. [Q9LV77-2]
NP_851272.1. NM_180941.2. [Q9LV77-1]
UniGeneiAt.67717.
At.7860.

Genome annotation databases

EnsemblPlantsiAT5G65010.1; AT5G65010.1; AT5G65010. [Q9LV77-1]
GeneIDi836625.
KEGGiath:AT5G65010.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095453 mRNA. Translation: AAC72837.1.
AB019236 Genomic DNA. Translation: BAA97313.1.
CP002688 Genomic DNA. Translation: AED97984.1.
CP002688 Genomic DNA. Translation: AED97985.1.
AF367340 mRNA. Translation: AAK32927.1.
AY124866 mRNA. Translation: AAM70575.1.
RefSeqiNP_201306.2. NM_125900.2. [Q9LV77-2]
NP_851272.1. NM_180941.2. [Q9LV77-1]
UniGeneiAt.67717.
At.7860.

3D structure databases

ProteinModelPortaliQ9LV77.
SMRiQ9LV77. Positions 18-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G65010.2.

Proteomic databases

PRIDEiQ9LV77.
ProMEXiQ9LV77.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G65010.1; AT5G65010.1; AT5G65010. [Q9LV77-1]
GeneIDi836625.
KEGGiath:AT5G65010.

Organism-specific databases

TAIRiAT5G65010.

Phylogenomic databases

HOGENOMiHOG000027493.
InParanoidiQ9LV77.
KOiK01953.
OMAiSRERVYW.
PhylomeDBiQ9LV77.

Enzyme and pathway databases

UniPathwayiUPA00134.
BioCyciARA:GQT-858-MONOMER.
ReactomeiREACT_276185. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

PROiQ9LV77.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Reciprocal regulation of distinct asparagine synthetase genes by light and metabolites in Arabidopsis thaliana."
    Lam H.M., Hsieh M.H., Coruzzi G.
    Plant J. 16:345-353(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INDUCTION.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
    DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Correlation of ASN2 gene expression with ammonium metabolism in Arabidopsis."
    Wong H.K., Chan H.K., Coruzzi G.M., Lam H.M.
    Plant Physiol. 134:332-338(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "An Arabidopsis mutant disrupted in ASN2 encoding asparagine synthetase 2 exhibits low salt stress tolerance."
    Maaroufi-Dguimi H., Debouba M., Gaufichon L., Clement G., Gouia H., Hajjaji A., Suzuki A.
    Plant Physiol. Biochem. 49:623-628(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Arabidopsis thaliana ASN2 encoding asparagine synthetase is involved in the control of nitrogen assimilation and export during vegetative growth."
    Gaufichon L., Masclaux-Daubresse C., Tcherkez G., Reisdorf-Cren M., Sakakibara Y., Hase T., Clement G., Avice J.C., Grandjean O., Marmagne A., Boutet-Mercey S., Azzopardi M., Soulay F., Suzuki A.
    Plant Cell Environ. 36:328-342(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiASNS2_ARATH
AccessioniPrimary (citable) accession number: Q9LV77
Secondary accession number(s): F4KGG8, Q9ZST6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.