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Protein

Proline-rich receptor-like protein kinase PERK1

Gene

PERK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei308 – 3081ATPPROSITE-ProRule annotation
Active sitei404 – 4041Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 2949ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • protein autophosphorylation Source: TAIR
  • response to fungus Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G24550-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich receptor-like protein kinase PERK1 (EC:2.7.11.1)
Alternative name(s):
Proline-rich extensin-like receptor kinase 1
Short name:
AtPERK1
Gene namesi
Name:PERK1
Ordered Locus Names:At3g24550
ORF Names:MOB24.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G24550.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 139139ExtracellularSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Topological domaini161 – 652492CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 652652Proline-rich receptor-like protein kinase PERK1PRO_0000400053Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence analysis
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei438 – 4381PhosphothreonineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LV48.
PRIDEiQ9LV48.

PTM databases

iPTMnetiQ9LV48.
SwissPalmiQ9LV48.

Expressioni

Tissue specificityi

Mostly expressed in inflorescence bolt, flower buds and siliques, and, to a lower extent, in roots, seedlings and leaves.1 Publication

Gene expression databases

GenevisibleiQ9LV48. AT.

Interactioni

Protein-protein interaction databases

BioGridi7382. 1 interaction.
STRINGi3702.AT3G24550.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LV48.
SMRiQ9LV48. Positions 266-590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 559280Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 257253Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LV48.
OMAiNTREMEM.
PhylomeDBiQ9LV48.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LV48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAPSPGTT PSPSPPSPPT NSTTTTPPPA ASSPPPTTTP SSPPPSPSTN
60 70 80 90 100
STSPPPSSPL PPSLPPPSPP GSLTPPLPQP SPSAPITPSP PSPTTPSNPR
110 120 130 140 150
SPPSPNQGPP NTPSGSTPRT PSNTKPSPPS DSSDGLSTGV VVGIAIGGVA
160 170 180 190 200
ILVILTLICL LCKKKRRRRH DDEAAYYVPP PPPSGPKAGG PYGGQQQYWQ
210 220 230 240 250
QQNASRPSDN HVVTSLPPPK PPSPPRKPPP PPPPPAFMSS SGGSDYSDLP
260 270 280 290 300
VLPPPSPGLV LGFSKSTFTY EELSRATNGF SEANLLGQGG FGYVHKGILP
310 320 330 340 350
SGKEVAVKQL KAGSGQGERE FQAEVEIISR VHHRHLVSLI GYCMAGVQRL
360 370 380 390 400
LVYEFVPNNN LEFHLHGKGR PTMEWSTRLK IALGSAKGLS YLHEDCNPKI
410 420 430 440 450
IHRDIKASNI LIDFKFEAKV ADFGLAKIAS DTNTHVSTRV MGTFGYLAPE
460 470 480 490 500
YAASGKLTEK SDVFSFGVVL LELITGRRPV DANNVYVDDS LVDWARPLLN
510 520 530 540 550
RASEEGDFEG LADSKMGNEY DREEMARMVA CAAACVRHSA RRRPRMSQIV
560 570 580 590 600
RALEGNVSLS DLNEGMRPGH SNVYSSYGGS TDYDTSQYND DMIKFRKMAL
610 620 630 640 650
GTQEYGTTGE YSNPTSDYGL YPSGSSSEGQ ATREMEMGKI KKTGQGYSGP

SL
Length:652
Mass (Da):69,272
Last modified:October 1, 2000 - v1
Checksum:i35005EE29FE8602F
GO
Isoform 2 (identifier: Q9LV48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-54: Missing.

Show »
Length:615
Mass (Da):65,747
Checksum:i2ECF169DBDA5F729
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771L → I in AAK43886 (PubMed:14593172).Curated
Sequence conflicti77 – 771L → I in AAP37768 (PubMed:14593172).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 5437Missing in isoform 2. 1 PublicationVSP_039986Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020746 Genomic DNA. Translation: BAB02007.1.
CP002686 Genomic DNA. Translation: AEE76920.1.
AF370509 mRNA. Translation: AAK43886.1.
AY056788 mRNA. Translation: AAL10479.1.
AY059901 mRNA. Translation: AAL24383.1.
AY093065 mRNA. Translation: AAM13064.1.
AY128792 mRNA. Translation: AAM91192.1.
BT008400 mRNA. Translation: AAP37759.1.
BT008409 mRNA. Translation: AAP37768.1.
AK319105 mRNA. Translation: BAH57220.1.
AY536855 mRNA. Translation: AAS65793.1.
AY536856 mRNA. Translation: AAS65794.1.
RefSeqiNP_189098.1. NM_113366.2. [Q9LV48-1]
UniGeneiAt.21723.

Genome annotation databases

EnsemblPlantsiAT3G24550.1; AT3G24550.1; AT3G24550. [Q9LV48-1]
GeneIDi822051.
KEGGiath:AT3G24550.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020746 Genomic DNA. Translation: BAB02007.1.
CP002686 Genomic DNA. Translation: AEE76920.1.
AF370509 mRNA. Translation: AAK43886.1.
AY056788 mRNA. Translation: AAL10479.1.
AY059901 mRNA. Translation: AAL24383.1.
AY093065 mRNA. Translation: AAM13064.1.
AY128792 mRNA. Translation: AAM91192.1.
BT008400 mRNA. Translation: AAP37759.1.
BT008409 mRNA. Translation: AAP37768.1.
AK319105 mRNA. Translation: BAH57220.1.
AY536855 mRNA. Translation: AAS65793.1.
AY536856 mRNA. Translation: AAS65794.1.
RefSeqiNP_189098.1. NM_113366.2. [Q9LV48-1]
UniGeneiAt.21723.

3D structure databases

ProteinModelPortaliQ9LV48.
SMRiQ9LV48. Positions 266-590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7382. 1 interaction.
STRINGi3702.AT3G24550.1.

PTM databases

iPTMnetiQ9LV48.
SwissPalmiQ9LV48.

Proteomic databases

PaxDbiQ9LV48.
PRIDEiQ9LV48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G24550.1; AT3G24550.1; AT3G24550. [Q9LV48-1]
GeneIDi822051.
KEGGiath:AT3G24550.

Organism-specific databases

TAIRiAT3G24550.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LV48.
OMAiNTREMEM.
PhylomeDBiQ9LV48.

Enzyme and pathway databases

BioCyciARA:AT3G24550-MONOMER.

Miscellaneous databases

PROiQ9LV48.

Gene expression databases

GenevisibleiQ9LV48. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  5. "Protein kinases in chloroplasts."
    Kurth J., Leister D.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-224 AND 424-646 (ISOFORM 1).
    Strain: cv. Columbia.
  6. "The proline-rich, extensin-like receptor kinase-1 (PERK1) gene is rapidly induced by wounding."
    Silva N.F., Goring D.R.
    Plant Mol. Biol. 50:667-685(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "A comprehensive expression analysis of the Arabidopsis proline-rich extensin-like receptor kinase gene family using bioinformatic and experimental approaches."
    Nakhamchik A., Zhao Z., Provart N.J., Shiu S.-H., Keatley S.K., Cameron R.K., Goring D.R.
    Plant Cell Physiol. 45:1875-1881(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "A high content in lipid-modified peripheral proteins and integral receptor kinases features in the arabidopsis plasma membrane proteome."
    Marmagne A., Ferro M., Meinnel T., Bruley C., Kuhn L., Garin J., Barbier-Brygoo H., Ephritikhine G.
    Mol. Cell. Proteomics 6:1980-1996(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPERK1_ARATH
AccessioniPrimary (citable) accession number: Q9LV48
Secondary accession number(s): C0Z3E1
, Q6QJ27, Q6QJ28, Q94JZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.