Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proline-rich receptor-like protein kinase PERK1

Gene

PERK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei308ATPPROSITE-ProRule annotation1
Active sitei404Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 294ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

  • protein autophosphorylation Source: TAIR
  • response to fungus Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich receptor-like protein kinase PERK1 (EC:2.7.11.1)
Alternative name(s):
Proline-rich extensin-like receptor kinase 1
Short name:
AtPERK1
Gene namesi
Name:PERK1
Ordered Locus Names:At3g24550
ORF Names:MOB24.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G24550.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 139ExtracellularSequence analysisAdd BLAST139
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 652CytoplasmicSequence analysisAdd BLAST492

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004000531 – 652Proline-rich receptor-like protein kinase PERK1Add BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...)Sequence analysis1
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
Modified residuei437PhosphoserineBy similarity1
Modified residuei438PhosphothreonineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LV48.

PTM databases

iPTMnetiQ9LV48.
SwissPalmiQ9LV48.

Expressioni

Tissue specificityi

Mostly expressed in inflorescence bolt, flower buds and siliques, and, to a lower extent, in roots, seedlings and leaves.1 Publication

Gene expression databases

GenevisibleiQ9LV48. AT.

Interactioni

Protein-protein interaction databases

BioGridi7382. 1 interactor.
STRINGi3702.AT3G24550.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LV48.
SMRiQ9LV48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 559Protein kinasePROSITE-ProRule annotationAdd BLAST280

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 257Pro-richAdd BLAST253

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LV48.
OMAiNTREMEM.
OrthoDBiEOG093609WD.
PhylomeDBiQ9LV48.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LV48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAPSPGTT PSPSPPSPPT NSTTTTPPPA ASSPPPTTTP SSPPPSPSTN
60 70 80 90 100
STSPPPSSPL PPSLPPPSPP GSLTPPLPQP SPSAPITPSP PSPTTPSNPR
110 120 130 140 150
SPPSPNQGPP NTPSGSTPRT PSNTKPSPPS DSSDGLSTGV VVGIAIGGVA
160 170 180 190 200
ILVILTLICL LCKKKRRRRH DDEAAYYVPP PPPSGPKAGG PYGGQQQYWQ
210 220 230 240 250
QQNASRPSDN HVVTSLPPPK PPSPPRKPPP PPPPPAFMSS SGGSDYSDLP
260 270 280 290 300
VLPPPSPGLV LGFSKSTFTY EELSRATNGF SEANLLGQGG FGYVHKGILP
310 320 330 340 350
SGKEVAVKQL KAGSGQGERE FQAEVEIISR VHHRHLVSLI GYCMAGVQRL
360 370 380 390 400
LVYEFVPNNN LEFHLHGKGR PTMEWSTRLK IALGSAKGLS YLHEDCNPKI
410 420 430 440 450
IHRDIKASNI LIDFKFEAKV ADFGLAKIAS DTNTHVSTRV MGTFGYLAPE
460 470 480 490 500
YAASGKLTEK SDVFSFGVVL LELITGRRPV DANNVYVDDS LVDWARPLLN
510 520 530 540 550
RASEEGDFEG LADSKMGNEY DREEMARMVA CAAACVRHSA RRRPRMSQIV
560 570 580 590 600
RALEGNVSLS DLNEGMRPGH SNVYSSYGGS TDYDTSQYND DMIKFRKMAL
610 620 630 640 650
GTQEYGTTGE YSNPTSDYGL YPSGSSSEGQ ATREMEMGKI KKTGQGYSGP

SL
Length:652
Mass (Da):69,272
Last modified:October 1, 2000 - v1
Checksum:i35005EE29FE8602F
GO
Isoform 2 (identifier: Q9LV48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-54: Missing.

Show »
Length:615
Mass (Da):65,747
Checksum:i2ECF169DBDA5F729
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77L → I in AAK43886 (PubMed:14593172).Curated1
Sequence conflicti77L → I in AAP37768 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03998618 – 54Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020746 Genomic DNA. Translation: BAB02007.1.
CP002686 Genomic DNA. Translation: AEE76920.1.
AF370509 mRNA. Translation: AAK43886.1.
AY056788 mRNA. Translation: AAL10479.1.
AY059901 mRNA. Translation: AAL24383.1.
AY093065 mRNA. Translation: AAM13064.1.
AY128792 mRNA. Translation: AAM91192.1.
BT008400 mRNA. Translation: AAP37759.1.
BT008409 mRNA. Translation: AAP37768.1.
AK319105 mRNA. Translation: BAH57220.1.
AY536855 mRNA. Translation: AAS65793.1.
AY536856 mRNA. Translation: AAS65794.1.
RefSeqiNP_189098.1. NM_113366.3. [Q9LV48-1]
UniGeneiAt.21723.

Genome annotation databases

EnsemblPlantsiAT3G24550.1; AT3G24550.1; AT3G24550. [Q9LV48-1]
GeneIDi822051.
GrameneiAT3G24550.1; AT3G24550.1; AT3G24550.
KEGGiath:AT3G24550.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020746 Genomic DNA. Translation: BAB02007.1.
CP002686 Genomic DNA. Translation: AEE76920.1.
AF370509 mRNA. Translation: AAK43886.1.
AY056788 mRNA. Translation: AAL10479.1.
AY059901 mRNA. Translation: AAL24383.1.
AY093065 mRNA. Translation: AAM13064.1.
AY128792 mRNA. Translation: AAM91192.1.
BT008400 mRNA. Translation: AAP37759.1.
BT008409 mRNA. Translation: AAP37768.1.
AK319105 mRNA. Translation: BAH57220.1.
AY536855 mRNA. Translation: AAS65793.1.
AY536856 mRNA. Translation: AAS65794.1.
RefSeqiNP_189098.1. NM_113366.3. [Q9LV48-1]
UniGeneiAt.21723.

3D structure databases

ProteinModelPortaliQ9LV48.
SMRiQ9LV48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7382. 1 interactor.
STRINGi3702.AT3G24550.1.

PTM databases

iPTMnetiQ9LV48.
SwissPalmiQ9LV48.

Proteomic databases

PaxDbiQ9LV48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G24550.1; AT3G24550.1; AT3G24550. [Q9LV48-1]
GeneIDi822051.
GrameneiAT3G24550.1; AT3G24550.1; AT3G24550.
KEGGiath:AT3G24550.

Organism-specific databases

TAIRiAT3G24550.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ9LV48.
OMAiNTREMEM.
OrthoDBiEOG093609WD.
PhylomeDBiQ9LV48.

Miscellaneous databases

PROiQ9LV48.

Gene expression databases

GenevisibleiQ9LV48. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPERK1_ARATH
AccessioniPrimary (citable) accession number: Q9LV48
Secondary accession number(s): C0Z3E1
, Q6QJ27, Q6QJ28, Q94JZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.