Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proline-rich receptor-like protein kinase PERK1

Gene

PERK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei308ATPPROSITE-ProRule annotation1
Active sitei404Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi286 – 294ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR
  • transmembrane receptor protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • protein autophosphorylation Source: TAIR
  • response to fungus Source: TAIR
  • response to wounding Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich receptor-like protein kinase PERK1 (EC:2.7.11.1)
Alternative name(s):
Proline-rich extensin-like receptor kinase 1
Short name:
AtPERK1
Gene namesi
Name:PERK1
Ordered Locus Names:At3g24550
ORF Names:MOB24.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G24550
TAIRilocus:2091722 AT3G24550

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 139ExtracellularSequence analysisAdd BLAST139
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 652CytoplasmicSequence analysisAdd BLAST492

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004000531 – 652Proline-rich receptor-like protein kinase PERK1Add BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei269PhosphothreonineBy similarity1
Modified residuei353PhosphotyrosineBy similarity1
Modified residuei408PhosphoserineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei438PhosphothreonineBy similarity1
Modified residuei443PhosphothreonineBy similarity1
Modified residuei451PhosphotyrosineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LV48
PRIDEiQ9LV48

PTM databases

iPTMnetiQ9LV48
SwissPalmiQ9LV48

Expressioni

Tissue specificityi

Mostly expressed in inflorescence bolt, flower buds and siliques, and, to a lower extent, in roots, seedlings and leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9LV48 baseline and differential
GenevisibleiQ9LV48 AT

Interactioni

Protein-protein interaction databases

BioGridi7382, 2 interactors
STRINGi3702.AT3G24550.1

Structurei

3D structure databases

ProteinModelPortaliQ9LV48
SMRiQ9LV48
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini280 – 559Protein kinasePROSITE-ProRule annotationAdd BLAST280

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 257Pro-richAdd BLAST253

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1187 Eukaryota
COG0515 LUCA
HOGENOMiHOG000116550
InParanoidiQ9LV48
OMAiFRKMALA
OrthoDBiEOG093609WD
PhylomeDBiQ9LV48

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9LV48-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAPSPGTT PSPSPPSPPT NSTTTTPPPA ASSPPPTTTP SSPPPSPSTN
60 70 80 90 100
STSPPPSSPL PPSLPPPSPP GSLTPPLPQP SPSAPITPSP PSPTTPSNPR
110 120 130 140 150
SPPSPNQGPP NTPSGSTPRT PSNTKPSPPS DSSDGLSTGV VVGIAIGGVA
160 170 180 190 200
ILVILTLICL LCKKKRRRRH DDEAAYYVPP PPPSGPKAGG PYGGQQQYWQ
210 220 230 240 250
QQNASRPSDN HVVTSLPPPK PPSPPRKPPP PPPPPAFMSS SGGSDYSDLP
260 270 280 290 300
VLPPPSPGLV LGFSKSTFTY EELSRATNGF SEANLLGQGG FGYVHKGILP
310 320 330 340 350
SGKEVAVKQL KAGSGQGERE FQAEVEIISR VHHRHLVSLI GYCMAGVQRL
360 370 380 390 400
LVYEFVPNNN LEFHLHGKGR PTMEWSTRLK IALGSAKGLS YLHEDCNPKI
410 420 430 440 450
IHRDIKASNI LIDFKFEAKV ADFGLAKIAS DTNTHVSTRV MGTFGYLAPE
460 470 480 490 500
YAASGKLTEK SDVFSFGVVL LELITGRRPV DANNVYVDDS LVDWARPLLN
510 520 530 540 550
RASEEGDFEG LADSKMGNEY DREEMARMVA CAAACVRHSA RRRPRMSQIV
560 570 580 590 600
RALEGNVSLS DLNEGMRPGH SNVYSSYGGS TDYDTSQYND DMIKFRKMAL
610 620 630 640 650
GTQEYGTTGE YSNPTSDYGL YPSGSSSEGQ ATREMEMGKI KKTGQGYSGP

SL
Length:652
Mass (Da):69,272
Last modified:October 1, 2000 - v1
Checksum:i35005EE29FE8602F
GO
Isoform 2 (identifier: Q9LV48-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-54: Missing.

Show »
Length:615
Mass (Da):65,747
Checksum:i2ECF169DBDA5F729
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77L → I in AAK43886 (PubMed:14593172).Curated1
Sequence conflicti77L → I in AAP37768 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03998618 – 54Missing in isoform 2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020746 Genomic DNA Translation: BAB02007.1
CP002686 Genomic DNA Translation: AEE76920.1
AF370509 mRNA Translation: AAK43886.1
AY056788 mRNA Translation: AAL10479.1
AY059901 mRNA Translation: AAL24383.1
AY093065 mRNA Translation: AAM13064.1
AY128792 mRNA Translation: AAM91192.1
BT008400 mRNA Translation: AAP37759.1
BT008409 mRNA Translation: AAP37768.1
AK319105 mRNA Translation: BAH57220.1
AY536855 mRNA Translation: AAS65793.1
AY536856 mRNA Translation: AAS65794.1
RefSeqiNP_189098.1, NM_113366.3 [Q9LV48-1]
UniGeneiAt.21723

Genome annotation databases

EnsemblPlantsiAT3G24550.1; AT3G24550.1; AT3G24550 [Q9LV48-1]
GeneIDi822051
GrameneiAT3G24550.1; AT3G24550.1; AT3G24550 [Q9LV48-1]
KEGGiath:AT3G24550

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPERK1_ARATH
AccessioniPrimary (citable) accession number: Q9LV48
Secondary accession number(s): C0Z3E1
, Q6QJ27, Q6QJ28, Q94JZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health