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Protein

Mitogen-activated protein kinase 9

Gene

MPK9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATPPROSITE-ProRule annotation
Active sitei149 – 1491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G18040-MONOMER.
ARA:GQT-1685-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 9 (EC:2.7.11.24)
Short name:
AtMPK9
Short name:
MAP kinase 9
Gene namesi
Name:MPK9
Ordered Locus Names:At3g18040
ORF Names:MRC8.2, MRC8.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G18040.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • mitochondrion Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 510510Mitogen-activated protein kinase 9PRO_0000245809Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei185 – 1851PhosphothreonineBy similarity
Modified residuei187 – 1871PhosphotyrosineBy similarity
Modified residuei190 – 1901PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LV37.
PRIDEiQ9LV37.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LV37. baseline and differential.

Interactioni

Protein-protein interaction databases

MINTiMINT-8060599.
STRINGi3702.AT3G18040.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LV37.
SMRiQ9LV37. Positions 23-374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 314292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi185 – 1873TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9LV37.
OMAiMELMEAN.
PhylomeDBiQ9LV37.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LV37-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPHKKVALE TEFFTEYGEA SRYQIQEVIG KGSYGVVASA IDTHSGEKVA
60 70 80 90 100
IKKINDVFEH VSDATRILRE IKLLRLLRHP DIVEIKHVML PPSRREFRDI
110 120 130 140 150
YVVFELMESD LHQVIKANDD LTPEHYQFFL YQLLRGLKFI HTANVFHRDL
160 170 180 190 200
KPKNILANSD CKLKICDFGL ARVSFNDAPS AIFWTDYVAT RWYRAPELCG
210 220 230 240 250
SFFSKYTPAI DIWSIGCIFA EMLTGKPLFP GKNVVHQLDI MTDLLGTPPP
260 270 280 290 300
EAIARIRNEK ARRYLGNMRR KPPVPFTHKF PHVDPLALRL LHRLLAFDPK
310 320 330 340 350
DRPSAEEALA DPYFYGLANV DREPSTQPIP KLEFEFERRK ITKEDVRELI
360 370 380 390 400
YREILEYHPQ MLQEYLRGGE QTSFMYPSGV DRFKRQFAHL EENYGKGEKG
410 420 430 440 450
SPLQRQHASL PRERVPAPKK ENGSHNHDIE NRSIASLVTT LESPPTSQHE
460 470 480 490 500
GSDYRNGTSQ TGYSARSLLK SASISASKCI GMKPRNKSEY GESNNDTVDA
510
LSQKVAALHT
Length:510
Mass (Da):58,395
Last modified:July 11, 2006 - v2
Checksum:i6E41FB646C3C7F12
GO

Sequence cautioni

The sequence BAB02016.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461G → C in BAA92223 (Ref. 1) Curated
Sequence conflicti54 – 541I → S in BAA92223 (Ref. 1) Curated
Sequence conflicti96 – 961E → D in BAA92223 (Ref. 1) Curated
Sequence conflicti190 – 1901T → N in BAA92223 (Ref. 1) Curated
Sequence conflicti341 – 3411I → L in BAA92223 (Ref. 1) Curated
Sequence conflicti461 – 4611T → Q in BAA92223 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038694 mRNA. Translation: BAA92223.1.
AB020749 Genomic DNA. Translation: BAB02016.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE76038.1.
RefSeqiNP_566595.1. NM_112686.4. [Q9LV37-1]
UniGeneiAt.471.

Genome annotation databases

EnsemblPlantsiAT3G18040.1; AT3G18040.1; AT3G18040. [Q9LV37-1]
GeneIDi821329.
KEGGiath:AT3G18040.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038694 mRNA. Translation: BAA92223.1.
AB020749 Genomic DNA. Translation: BAB02016.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE76038.1.
RefSeqiNP_566595.1. NM_112686.4. [Q9LV37-1]
UniGeneiAt.471.

3D structure databases

ProteinModelPortaliQ9LV37.
SMRiQ9LV37. Positions 23-374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8060599.
STRINGi3702.AT3G18040.1.

Proteomic databases

PaxDbiQ9LV37.
PRIDEiQ9LV37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G18040.1; AT3G18040.1; AT3G18040. [Q9LV37-1]
GeneIDi821329.
KEGGiath:AT3G18040.

Organism-specific databases

GeneFarmi865. 89.
TAIRiAT3G18040.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ9LV37.
OMAiMELMEAN.
PhylomeDBiQ9LV37.

Enzyme and pathway databases

BioCyciARA:AT3G18040-MONOMER.
ARA:GQT-1685-MONOMER.

Miscellaneous databases

PROiQ9LV37.

Gene expression databases

ExpressionAtlasiQ9LV37. baseline and differential.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana mRNA for MAP kinase."
    Mizoguchi T., Ichimura K., Shinozaki K.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. Cited for: GENE FAMILY.

Entry informationi

Entry nameiMPK9_ARATH
AccessioniPrimary (citable) accession number: Q9LV37
Secondary accession number(s): Q9MB22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: June 24, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.