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Protein

Mitogen-activated protein kinase 9

Gene

MPK9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52ATPPROSITE-ProRule annotation1
Active sitei149Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 37ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • regulation of gene expression Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 9 (EC:2.7.11.24)
Short name:
AtMPK9
Short name:
MAP kinase 9
Gene namesi
Name:MPK9
Ordered Locus Names:At3g18040
ORF Names:MRC8.2, MRC8.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G18040.
TAIRilocus:2092717. AT3G18040.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458091 – 510Mitogen-activated protein kinase 9Add BLAST510

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei185PhosphothreonineBy similarity1
Modified residuei187PhosphotyrosineBy similarity1
Modified residuei190PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9LV37.

PTM databases

iPTMnetiQ9LV37.

Expressioni

Gene expression databases

ExpressionAtlasiQ9LV37. baseline and differential.
GenevisibleiQ9LV37. AT.

Interactioni

Protein-protein interaction databases

BioGridi6662. 2 interactors.
MINTiMINT-8060599.
STRINGi3702.AT3G18040.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LV37.
SMRiQ9LV37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 314Protein kinasePROSITE-ProRule annotationAdd BLAST292

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi185 – 187TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ9LV37.
OMAiKYTPGID.
OrthoDBiEOG093605CF.
PhylomeDBiQ9LV37.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9LV37-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPHKKVALE TEFFTEYGEA SRYQIQEVIG KGSYGVVASA IDTHSGEKVA
60 70 80 90 100
IKKINDVFEH VSDATRILRE IKLLRLLRHP DIVEIKHVML PPSRREFRDI
110 120 130 140 150
YVVFELMESD LHQVIKANDD LTPEHYQFFL YQLLRGLKFI HTANVFHRDL
160 170 180 190 200
KPKNILANSD CKLKICDFGL ARVSFNDAPS AIFWTDYVAT RWYRAPELCG
210 220 230 240 250
SFFSKYTPAI DIWSIGCIFA EMLTGKPLFP GKNVVHQLDI MTDLLGTPPP
260 270 280 290 300
EAIARIRNEK ARRYLGNMRR KPPVPFTHKF PHVDPLALRL LHRLLAFDPK
310 320 330 340 350
DRPSAEEALA DPYFYGLANV DREPSTQPIP KLEFEFERRK ITKEDVRELI
360 370 380 390 400
YREILEYHPQ MLQEYLRGGE QTSFMYPSGV DRFKRQFAHL EENYGKGEKG
410 420 430 440 450
SPLQRQHASL PRERVPAPKK ENGSHNHDIE NRSIASLVTT LESPPTSQHE
460 470 480 490 500
GSDYRNGTSQ TGYSARSLLK SASISASKCI GMKPRNKSEY GESNNDTVDA
510
LSQKVAALHT
Length:510
Mass (Da):58,395
Last modified:July 11, 2006 - v2
Checksum:i6E41FB646C3C7F12
GO

Sequence cautioni

The sequence BAB02016 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46G → C in BAA92223 (Ref. 1) Curated1
Sequence conflicti54I → S in BAA92223 (Ref. 1) Curated1
Sequence conflicti96E → D in BAA92223 (Ref. 1) Curated1
Sequence conflicti190T → N in BAA92223 (Ref. 1) Curated1
Sequence conflicti341I → L in BAA92223 (Ref. 1) Curated1
Sequence conflicti461T → Q in BAA92223 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038694 mRNA. Translation: BAA92223.1.
AB020749 Genomic DNA. Translation: BAB02016.1. Different initiation.
CP002686 Genomic DNA. Translation: AEE76038.1.
RefSeqiNP_001327298.1. NM_001338320.1.
NP_566595.1. NM_112686.4. [Q9LV37-1]
UniGeneiAt.471.

Genome annotation databases

EnsemblPlantsiAT3G18040.1; AT3G18040.1; AT3G18040. [Q9LV37-1]
AT3G18040.4; AT3G18040.4; AT3G18040.
GeneIDi821329.
GrameneiAT3G18040.1; AT3G18040.1; AT3G18040.
AT3G18040.4; AT3G18040.4; AT3G18040.
KEGGiath:AT3G18040.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMPK9_ARATH
AccessioniPrimary (citable) accession number: Q9LV37
Secondary accession number(s): Q9MB22
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: November 22, 2017
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families