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Q9LV34 (BGL43_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 43

Short name=AtBGLU43
EC=3.2.1.21
Gene names
Name:BGLU43
Ordered Locus Names:At3g18070
ORF Names:MRC8.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence BAB02019.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9LV34-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Derived from EST data. No experimental confirmation available.
Isoform 2 (identifier: Q9LV34-2)

The sequence of this isoform differs from the canonical sequence as follows:
     92-168: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Potential
Chain15 – 501487Beta-glucosidase 43
PRO_0000390316

Regions

Region462 – 4632Substrate binding By similarity

Sites

Active site1941Proton donor By similarity
Active site4081Nucleophile By similarity
Binding site491Substrate By similarity
Binding site1501Substrate By similarity
Binding site1931Substrate By similarity
Binding site3361Substrate By similarity
Binding site4551Substrate By similarity

Amino acid modifications

Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation2191N-linked (GlcNAc...) Potential
Glycosylation3601N-linked (GlcNAc...) Potential
Glycosylation4161N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation4481N-linked (GlcNAc...) Potential
Disulfide bond213 ↔ 220 By similarity

Natural variations

Alternative sequence92 – 16877Missing in isoform 2.
VSP_038500

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 2009. Version 2.
Checksum: 755B6F26B32B481E

FASTA50157,244
        10         20         30         40         50         60 
MFLFLLLLSA SRSGEESPSG DAVPLATGGL NRKSFPEGFL FGTATSAYQV EGETHQDGRG 

        70         80         90        100        110        120 
PSIWDAFVKI PGKIANNATA EITVDQYHRY KEDVDLMQNL NIDAYRFSIS WSRIFPEGSG 

       130        140        150        160        170        180 
KINSNGVAYY NRLIDYLIEK GITPYANLYH YDLPLALEQK YQGLLSKQGR FCGLRRVLFQ 

       190        200        210        220        230        240 
TFGDRVKNWM TFNEPRVVAA LGYDNGIFAP GRCSEAFGNC TDGNSATEPY IVAHHLILAH 

       250        260        270        280        290        300 
AAAVQRYRQN YQEKQKGRVG ILLDFVWFEP LTSSQADNDA AQRARDFHVG WFIHPIVYGE 

       310        320        330        340        350        360 
YPNTLQNIVK ERLPKFTEEE VKMVKGSIDF VGINQYTTYF MSDPKISTTP KDLGYQQDWN 

       370        380        390        400        410        420 
VTFNFAKNGT PIGPRAHSEW LYNVPWGMYK ALMYIEERYG NPTMILSENG MDDPGNITLT 

       430        440        450        460        470        480 
QGLNDTTRVK YYRDYLVQLK KAVDDGANLT GYFAWSLLDN FEWLSGYTSR FGIVYVDYKD 

       490        500 
LKRYPKMSAL WFKQLLKRDQ K 

« Hide

Isoform 2 [UniParc].

Checksum: 552610A1B3A40EF5
Show »

FASTA42448,313

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB020749 Genomic DNA. Translation: BAB02019.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76042.1.
CP002686 Genomic DNA. Translation: AEE76043.1.
DQ446670 mRNA. Translation: ABE65945.1.
RefSeqNP_001078176.1. NM_001084707.1.
NP_188435.2. NM_112689.2.
UniGeneAt.53364.

3D structure databases

ProteinModelPortalQ9LV34.
SMRQ9LV34. Positions 28-496.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ9LV34.
PRIDEQ9LV34.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G18070.1; AT3G18070.1; AT3G18070. [Q9LV34-1]
GeneID821332.
KEGGath:AT3G18070.

Organism-specific databases

TAIRAT3G18070.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidQ1PEP7.
OMAGKIANNA.
PhylomeDBQ9LV34.
ProtClustDBCLSN2684361.

Enzyme and pathway databases

BioCycARA:AT3G18070-MONOMER.
ARA:GQT-414-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGL43_ARATH
AccessionPrimary (citable) accession number: Q9LV34
Secondary accession number(s): Q1PEP7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: December 15, 2009
Last modified: April 16, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names