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Q9LV33 (BGL44_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 44

Short name=AtBGLU44
EC=3.2.1.21
Gene names
Name:BGLU44
Ordered Locus Names:At3g18080
ORF Names:MRC8.20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, cellobiose, 4-methylumbelliferyl-beta-D-glucoside, laminarin, amygdalin, esculin and gentiobiose. Ref.5

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Subunit structure

Homodimer. Formation of the homodimer is zinc-dependent Potential.

Subcellular location

Secreted Potential.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 512489Beta-glucosidase 44
PRO_0000383462

Regions

Region473 – 4742Substrate binding By similarity

Sites

Active site2051Proton donor By similarity
Active site4191Nucleophile By similarity
Metal binding941Zinc; shared with dimeric partner Potential
Metal binding971Zinc; shared with dimeric partner Potential
Binding site581Substrate By similarity
Binding site1591Substrate By similarity
Binding site2041Substrate By similarity
Binding site3471Substrate By similarity
Binding site4661Substrate By similarity

Amino acid modifications

Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation2301N-linked (GlcNAc...) Potential
Glycosylation4271N-linked (GlcNAc...) Potential
Disulfide bond224 ↔ 231 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LV33 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: A870F9BB20C342E7

FASTA51258,983
        10         20         30         40         50         60 
MRHLSSPPWP LLLLLLLSSF TSGESSLSAE KNKLHTGGLS RQSFPKGFVF GTATSAYQVE 

        70         80         90        100        110        120 
GETHQDGRGP SIWDAFVKIP GKIAKNATAE ITVDQYHRYK EDVDLMKKLN FDAYRFSISW 

       130        140        150        160        170        180 
SRIFPEGSGK VNWKGVAYYN RLIDYMVQKG ITPYANLYHY DLPLALENKY KGLLGRQVVK 

       190        200        210        220        230        240 
DFADYAEFCY KTFGDRVKNW MTFNEPRVVA ALGYDNGIFA PGRCSKAFGN CTEGNSATEP 

       250        260        270        280        290        300 
YIVTHHLILA HAAAVQRYRK YYQAKQKGRV GILLDFVWYE PLTRSKADNL AAQRARDFHI 

       310        320        330        340        350        360 
GWFIHPLVYG EYPKTMQNIV KERLPKFTEK EVKMVKGSID FVGINQYTTY YMSEPHPTTK 

       370        380        390        400        410        420 
PKDLGYQQDW NVEFGFAKLG KPIGPRAYSS WLYNVPWGMY KALMYMKERY GNPTMILSEN 

       430        440        450        460        470        480 
GMDDPGNVTL AQGLHDTTRI KYYKDYLTNL KKARDDGANV VGYFAWSLLD NFEWLSGYTS 

       490        500        510 
RFGIVYVDYK TLKRYPKMSA QWFKQLLKRN NK 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB020749 Genomic DNA. Translation: BAB02020.1.
CP002686 Genomic DNA. Translation: AEE76044.1.
AK316840 mRNA. Translation: BAH19552.1.
AK316900 mRNA. Translation: BAH19607.1.
AY084864 mRNA. Translation: AAM61427.1.
RefSeqNP_188436.1. NM_112690.3.
UniGeneAt.21649.

3D structure databases

ProteinModelPortalQ9LV33.
SMRQ9LV33. Positions 37-507.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G18080.1-P.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbQ9LV33.
PRIDEQ9LV33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G18080.1; AT3G18080.1; AT3G18080.
GeneID821333.
KEGGath:AT3G18080.

Organism-specific databases

TAIRAT3G18080.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidQ9LV33.
KOK05350.
OMAGKVNWKG.
PhylomeDBQ9LV33.
ProtClustDBCLSN2684361.

Gene expression databases

GenevestigatorQ9LV33.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGL44_ARATH
AccessionPrimary (citable) accession number: Q9LV33
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names