Reviewed, UniProtKB/Swiss-Prot Q9LV33 (BGL44_ARATH)

Names and origin

Protein names

Recommended name:
    Beta-glucosidase 44
      Short name=AtBGLU44
    EC=3.2.1.21

Gene names

Name:	BGLU44
Ordered Locus Names:	At3g18080
ORF Names:	MRC8.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-mannoside, cellobiose, 4-methylumbelliferyl-beta-D-glucoside, laminarin, amygdalin, esculin and gentiobiose.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subunit structure	Homodimer. Formation of the homodimer is zinc-dependent Potential.
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosolic ribosome Inferred from direct assay. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	4-methylumbelliferyl-beta-D-glucopyranoside beta-glucosidase activity Ref.4 Inferred from direct assay. Source: TAIR amygdalin beta-glucosidase activity Ref.4 Inferred from direct assay. Source: TAIR beta-gentiobiose beta-glucosidase activity Ref.4 Inferred from direct assay. Source: TAIR beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro cellobiose glucosidase activity Ref.4 Inferred from direct assay. Source: TAIR esculin beta-glucosidase activity Ref.4 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 512	489	Beta-glucosidase 44		PRO_0000383462
Sites
	Active site	205	1	Proton donor By similarity
	Active site	419	1	Nucleophile By similarity
	Metal binding	94	1	Zinc; shared with dimeric partner Potential
	Metal binding	97	1	Zinc; shared with dimeric partner Potential
	Binding site	58	1	Substrate By similarity
	Binding site	204	1	Substrate By similarity
	Binding site	419	1	Substrate By similarity
	Binding site	473	1	Substrate By similarity
	Binding site	474	1	Substrate By similarity
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Glycosylation	427	1	N-linked (GlcNAc...) Potential
	Disulfide bond	224 ↔ 231		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9LV33-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A870F9BB20C342E7	FASTA	512	58,983
	10 20 30 40 50 60 MRHLSSPPWP LLLLLLLSSF TSGESSLSAE KNKLHTGGLS RQSFPKGFVF GTATSAYQVE 70 80 90 100 110 120 GETHQDGRGP SIWDAFVKIP GKIAKNATAE ITVDQYHRYK EDVDLMKKLN FDAYRFSISW 130 140 150 160 170 180 SRIFPEGSGK VNWKGVAYYN RLIDYMVQKG ITPYANLYHY DLPLALENKY KGLLGRQVVK 190 200 210 220 230 240 DFADYAEFCY KTFGDRVKNW MTFNEPRVVA ALGYDNGIFA PGRCSKAFGN CTEGNSATEP 250 260 270 280 290 300 YIVTHHLILA HAAAVQRYRK YYQAKQKGRV GILLDFVWYE PLTRSKADNL AAQRARDFHI 310 320 330 340 350 360 GWFIHPLVYG EYPKTMQNIV KERLPKFTEK EVKMVKGSID FVGINQYTTY YMSEPHPTTK 370 380 390 400 410 420 PKDLGYQQDW NVEFGFAKLG KPIGPRAYSS WLYNVPWGMY KALMYMKERY GNPTMILSEN 430 440 450 460 470 480 GMDDPGNVTL AQGLHDTTRI KYYKDYLTNL KKARDDGANV VGYFAWSLLD NFEWLSGYTS 490 500 510 RFGIVYVDYK TLKRYPKMSA QWFKQLLKRN NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed: 19423640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1." Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A. Plant Mol. Biol. 55:343-367(2004) [PubMed: 15604686] [Abstract] Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB020749 Genomic DNA. Translation: BAB02020.1. AK316840 mRNA. Translation: BAH19552.1. AK316900 mRNA. Translation: BAH19607.1. AY084864 mRNA. Translation: AAM61427.1.
IPI	IPI00537910.
RefSeq	NP_188436.1.
UniGene	At.21649
3D structure databases
HSSP	HSSP built from PDB template 1CBG based on UniProtKB P26205.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LV33.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Proteomic databases
PRIDE	Q9LV33.
Genome annotation databases
GeneID	821333.
GenomeReviews	Gene locus AT3G18080 in contig BA000014_GR.
KEGG	ath:AT3G18080.
NMPDR	fig|3702.1.peg.14003.
Organism-specific databases
TAIR	At3g18080.
Phylogenomic databases
OMA	NWKGVAY
PhylomeDB	Q9LV33.
Gene expression databases
Genevestigator	Q9LV33.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGL44_ARATH

Accession

Primary (citable) accession number: Q9LV33

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	October 1, 2000
Last modified:	November 24, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)