Q9LV16 (B3GTJ_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-1,3-galactosyltransferase 19
EC=2.4.1.-

Gene names

Name:	B3GALT19
Ordered Locus Names:	At5g62620
ORF Names:	MRG21.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal glycosyl residue By similarity.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed in junveile leaves and stems, and at lower levels in cauline leaves and siliques. Ref.4
Sequence similarities	Belongs to the glycosyltransferase 31 family. Contains 1 galectin domain.
Sequence caution	The sequence BAA97209.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BX831498 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosyltransferase activity Inferred from electronic annotation. Source: InterPro sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9LV16-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	Probable beta-1,3-galactosyltransferase 19		PRO_0000359429
Regions
	Transmembrane	29 – 49	21	Helical; Signal-anchor for type II membrane protein; Potential
	Domain	187 – 401	215	Galectin
Amino acid modifications
	Glycosylation	629	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 096DB0745ECDDE0B	FASTA	681	77,741
	10 20 30 40 50 60 MRKPKLSKLE RLEKFDIFVS LSKQRSVQIL MAVGLLYMLL ITFEIPFVFK TGLSSLSQDP 70 80 90 100 110 120 LTRPEKHNSQ RELQERRAPT RPLKSLLYQE SQSESPAQGL RRRTRILSSL RFDPETFNPS 130 140 150 160 170 180 SKDGSVELHK SAKVAWEVGR KIWEELESGK TLKALEKEKK KKIEEHGTNS CSLSVSLTGS 190 200 210 220 230 240 DLLKRGNIME LPCGLTLGSH ITVVGKPRAA HSEKDPKISM LKEGDEAVKV SQFKLELQGL 250 260 270 280 290 300 KAVEGEEPPR ILHLNPRLKG DWSGKPVIEQ NTCYRMQWGS AQRCEGWRSR DDEETVDGQV 310 320 330 340 350 360 KCEKWARDDS ITSKEEESSK AASWWLSRLI GRSKKVTVEW PFPFTVDKLF VLTLSAGLEG 370 380 390 400 410 420 YHVSVDGKHV TSFPYRTGFT LEDATGLTIN GDIDVHSVFA GSLPTSHPSF SPQRHLELSS 430 440 450 460 470 480 NWQAPSLPDE QVDMFIGILS AGNHFAERMA VRRSWMQHKL VKSSKVVARF FVALHSRKEV 490 500 510 520 530 540 NVELKKEAEF FGDIVIVPYM DSYDLVVLKT VAICEYGAHQ LAAKFIMKCD DDTFVQVDAV 550 560 570 580 590 600 LSEAKKTPTD RSLYIGNINY YHKPLRQGKW SVTYEEWPEE DYPPYANGPG YILSNDISRF 610 620 630 640 650 660 IVKEFEKHKL RMFKMEDVSV GMWVEQFNNG TKPVDYIHSL RFCQFGCIEN YLTAHYQSPR 670 680 QMICLWDKLV LTGKPQCCNM R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed: 14993207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"A unique beta-1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana." Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Gloessl J., Leonard R., Stadlmann J., Altmann F., Steinkellner H., Mach L. Plant Cell 19:2278-2292(2007) [PubMed: 17630273] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Identification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases." Qu Y., Egelund J., Gilson P.R., Houghton F., Gleeson P.A., Schultz C.J., Bacic A. Plant Mol. Biol. 68:43-59(2008) [PubMed: 18548197] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020751 Genomic DNA. Translation: BAA97209.1. Sequence problems. CP002688 Genomic DNA. Translation: AED97631.1. BX831498 mRNA. No translation available.
IPI	IPI00524772.
RefSeq	NP_201068.1. NM_125657.4.
UniGene	At.29039.
3D structure databases
ProteinModelPortal	Q9LV16.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LV16.
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Proteomic databases
PRIDE	Q9LV16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G62620.1; AT5G62620.1; AT5G62620.
GeneID	836383.
GenomeReviews	Gene locus AT5G62620 in contig BA000015_GR.
KEGG	ath:AT5G62620.
Organism-specific databases
TAIR	At5g62620.
Phylogenomic databases
GeneTree	EPGT00050000005432.
HOGENOM	HBG318248.
InParanoid	Q9LV16.
OMA	HSRKEVN.
PhylomeDB	Q9LV16.
ProtClustDB	CLSN2687350.
Gene expression databases
Genevestigator	Q9LV16.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR001079. Galectin_CRD. IPR002659. Glyco_trans_31. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 2 hits.
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF00337. Gal-bind_lectin. 1 hit. PF01762. Galactosyl_T. 1 hit. [Graphical view]
SMART	SM00908. Gal-bind_lectin. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS51304. GALECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GTJ_ARATH

Accession

Primary (citable) accession number: Q9LV16

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	December 14, 2011
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program