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Protein

Protein NRT1/ PTR FAMILY 2.11

Gene

NPF2.11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity, proton-dependent glucosinolate-specific transporter. Involved in apoplasmic phloem-loading of glucosinolates and in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots.2 Publications

Kineticsi

  1. KM=18.7 µM for 4-methylthiobutyl glucosinolate1 Publication

    GO - Molecular functioni

    • glucosinolate:proton symporter activity Source: TAIR

    GO - Biological processi

    • glucosinolate transport Source: TAIR
    • phloem glucosinolate loading Source: TAIR
    • response to karrikin Source: TAIR

    Keywordsi

    Biological processTransport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975 Proton/oligopeptide cotransporters
    R-ATH-6798695 Neutrophil degranulation

    Protein family/group databases

    TCDBi2.A.17.3.20 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 2.11
    Short name:
    AtNPF2.11
    Alternative name(s):
    Nitrate transporter 1.10
    Protein GLUCOSINOLATE TRANSPORTER-2
    Gene namesi
    Name:NPF2.11
    Synonyms:GTR2, NRT1.10
    Ordered Locus Names:At5g62680
    ORF Names:MRG21.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    AraportiAT5G62680
    TAIRilocus:2172249 AT5G62680

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
    Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
    Transmembranei118 – 138HelicalSequence analysisAdd BLAST21
    Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
    Transmembranei205 – 225HelicalSequence analysisAdd BLAST21
    Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
    Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
    Transmembranei392 – 412HelicalSequence analysisAdd BLAST21
    Transmembranei435 – 455HelicalSequence analysisAdd BLAST21
    Transmembranei483 – 503HelicalSequence analysisAdd BLAST21
    Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
    Transmembranei566 – 586HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    48% reduction in total glucosinolate levels in seeds. Gtr1 and gtr2 double mutant has no detectable glucosinolate in seeds.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003999871 – 616Protein NRT1/ PTR FAMILY 2.11Add BLAST616

    Proteomic databases

    PaxDbiQ9LV10
    PRIDEiQ9LV10

    Expressioni

    Tissue specificityi

    Expressed in roots. Detected in shoots, stems and flowers. Expressed in veins and in the root vasculature with highest expression in lateral branching points.3 Publications

    Gene expression databases

    ExpressionAtlasiQ9LV10 baseline and differential
    GenevisibleiQ9LV10 AT

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G62680.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LV10
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    HOGENOMiHOG000237399
    InParanoidiQ9LV10
    OMAiVWATCIV
    OrthoDBiEOG093606CO
    PhylomeDBiQ9LV10

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 1 hit
    SUPFAMiSSF103473 SSF103473, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q9LV10-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MERKPLELES TDNHQNPSSA VYGGSVTAVD SVEEDVQNQK KVVYRGWKVM
    60 70 80 90 100
    PFIIGNETFE KLGIIGTLSN LLVYLTAVFN LKSITAATII NAFSGTINFG
    110 120 130 140 150
    TFVAAFLCDT YFGRYKTLSV AVIACFLGSF VILLTAAVPQ LHPAACGTAA
    160 170 180 190 200
    DSICNGPSGG QIAFLLMGLG FLVVGAGGIR PCNLAFGADQ FNPKSESGKR
    210 220 230 240 250
    GIDSFFNWYF FTFTFAQILS LTLVVYVQSN VSWTIGLTIP AVLMFLACLI
    260 270 280 290 300
    FFAGDKLYVK IKASGSPLAG IAQVIAVAIK KRGLKPAKQP WLNLYNYYPP
    310 320 330 340 350
    KYANSKLKYT DQFRFLDKAA ILTPEDKLQP DGKPADPWKL CTMQQVEEVK
    360 370 380 390 400
    CIVRVLPIWF ASSIYYLTIT QQMTYPVFQA LQSDRRLGSG GFVIPAATYV
    410 420 430 440 450
    VFLMTGMTVF IVVYDRVLVP TMRRITGLDT GITLLQRIGT GIFFATASLV
    460 470 480 490 500
    VAGFVEERRR TFALTKPTLG MAPRKGEISS MSAMWLIPQL SLAGVAEAFA
    510 520 530 540 550
    AIGQMEFYYK QFPENMRSFA GSIFYVGGGV SSYLGSFLIA TVHRTTQNSS
    560 570 580 590 600
    GGNWLAEDLN KGRLDLFYFM IAGILAVNFA YFLVMSRWYR YKGSDDEVTT
    610
    YETNENIIKQ QDKNVA
    Length:616
    Mass (Da):67,891
    Last modified:October 1, 2000 - v1
    Checksum:i8D545D2664C3115C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB020751 Genomic DNA Translation: BAA97215.1
    CP002688 Genomic DNA Translation: AED97642.1
    AK316918 mRNA Translation: BAH19623.1
    RefSeqiNP_201074.1, NM_125663.4
    UniGeneiAt.28239
    At.68527

    Genome annotation databases

    EnsemblPlantsiAT5G62680.1; AT5G62680.1; AT5G62680
    GeneIDi836389
    GrameneiAT5G62680.1; AT5G62680.1; AT5G62680
    KEGGiath:AT5G62680

    Similar proteinsi

    Entry informationi

    Entry nameiPTR53_ARATH
    AccessioniPrimary (citable) accession number: Q9LV10
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 94 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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