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Protein

Protein NRT1/ PTR FAMILY 2.11

Gene

NPF2.11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity, proton-dependent glucosinolate-specific transporter. Involved in apoplasmic phloem-loading of glucosinolates and in bidirectional long-distance transport of aliphatic but not indole glucosinolates. May be involved in removal of glucosinolates from the xylem in roots.2 Publications

Kineticsi

  1. KM=18.7 µM for 4-methylthiobutyl glucosinolate1 Publication

    GO - Molecular functioni

    • glucosinolate:proton symporter activity Source: TAIR

    GO - Biological processi

    • glucosinolate transport Source: TAIR
    • phloem glucosinolate loading Source: TAIR
    • response to karrikin Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975. Proton/oligopeptide cotransporters.

    Protein family/group databases

    TCDBi2.A.17.3.20. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 2.11
    Short name:
    AtNPF2.11
    Alternative name(s):
    Nitrate transporter 1.10
    Protein GLUCOSINOLATE TRANSPORTER-2
    Gene namesi
    Name:NPF2.11
    Synonyms:GTR2, NRT1.10
    Ordered Locus Names:At5g62680
    ORF Names:MRG21.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 5

    Organism-specific databases

    TAIRiAT5G62680.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei59 – 7921HelicalSequence analysisAdd
    BLAST
    Transmembranei87 – 10721HelicalSequence analysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence analysisAdd
    BLAST
    Transmembranei159 – 17921HelicalSequence analysisAdd
    BLAST
    Transmembranei205 – 22521HelicalSequence analysisAdd
    BLAST
    Transmembranei233 – 25321HelicalSequence analysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence analysisAdd
    BLAST
    Transmembranei392 – 41221HelicalSequence analysisAdd
    BLAST
    Transmembranei435 – 45521HelicalSequence analysisAdd
    BLAST
    Transmembranei483 – 50321HelicalSequence analysisAdd
    BLAST
    Transmembranei519 – 53921HelicalSequence analysisAdd
    BLAST
    Transmembranei566 – 58621HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: TAIR
    • plasmodesma Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    48% reduction in total glucosinolate levels in seeds. Gtr1 and gtr2 double mutant has no detectable glucosinolate in seeds.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 616616Protein NRT1/ PTR FAMILY 2.11PRO_0000399987Add
    BLAST

    Proteomic databases

    PaxDbiQ9LV10.
    PRIDEiQ9LV10.

    PTM databases

    iPTMnetiQ9LV10.

    Expressioni

    Tissue specificityi

    Expressed in roots. Deteted in shoots, stems and flowers. Expressed in veins and in the root vasculature with highest expression in lateral branching points.3 Publications

    Gene expression databases

    GenevisibleiQ9LV10. AT.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT5G62680.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LV10.
    SMRiQ9LV10. Positions 46-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237399.
    InParanoidiQ9LV10.
    OMAiYLTITQQ.
    PhylomeDBiQ9LV10.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9LV10-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MERKPLELES TDNHQNPSSA VYGGSVTAVD SVEEDVQNQK KVVYRGWKVM
    60 70 80 90 100
    PFIIGNETFE KLGIIGTLSN LLVYLTAVFN LKSITAATII NAFSGTINFG
    110 120 130 140 150
    TFVAAFLCDT YFGRYKTLSV AVIACFLGSF VILLTAAVPQ LHPAACGTAA
    160 170 180 190 200
    DSICNGPSGG QIAFLLMGLG FLVVGAGGIR PCNLAFGADQ FNPKSESGKR
    210 220 230 240 250
    GIDSFFNWYF FTFTFAQILS LTLVVYVQSN VSWTIGLTIP AVLMFLACLI
    260 270 280 290 300
    FFAGDKLYVK IKASGSPLAG IAQVIAVAIK KRGLKPAKQP WLNLYNYYPP
    310 320 330 340 350
    KYANSKLKYT DQFRFLDKAA ILTPEDKLQP DGKPADPWKL CTMQQVEEVK
    360 370 380 390 400
    CIVRVLPIWF ASSIYYLTIT QQMTYPVFQA LQSDRRLGSG GFVIPAATYV
    410 420 430 440 450
    VFLMTGMTVF IVVYDRVLVP TMRRITGLDT GITLLQRIGT GIFFATASLV
    460 470 480 490 500
    VAGFVEERRR TFALTKPTLG MAPRKGEISS MSAMWLIPQL SLAGVAEAFA
    510 520 530 540 550
    AIGQMEFYYK QFPENMRSFA GSIFYVGGGV SSYLGSFLIA TVHRTTQNSS
    560 570 580 590 600
    GGNWLAEDLN KGRLDLFYFM IAGILAVNFA YFLVMSRWYR YKGSDDEVTT
    610
    YETNENIIKQ QDKNVA
    Length:616
    Mass (Da):67,891
    Last modified:October 1, 2000 - v1
    Checksum:i8D545D2664C3115C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB020751 Genomic DNA. Translation: BAA97215.1.
    CP002688 Genomic DNA. Translation: AED97642.1.
    AK316918 mRNA. Translation: BAH19623.1.
    RefSeqiNP_201074.1. NM_125663.3.
    UniGeneiAt.28239.
    At.68527.

    Genome annotation databases

    EnsemblPlantsiAT5G62680.1; AT5G62680.1; AT5G62680.
    GeneIDi836389.
    GrameneiAT5G62680.1; AT5G62680.1; AT5G62680.
    KEGGiath:AT5G62680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB020751 Genomic DNA. Translation: BAA97215.1.
    CP002688 Genomic DNA. Translation: AED97642.1.
    AK316918 mRNA. Translation: BAH19623.1.
    RefSeqiNP_201074.1. NM_125663.3.
    UniGeneiAt.28239.
    At.68527.

    3D structure databases

    ProteinModelPortaliQ9LV10.
    SMRiQ9LV10. Positions 46-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi3702.AT5G62680.1.

    Protein family/group databases

    TCDBi2.A.17.3.20. the proton-dependent oligopeptide transporter (pot/ptr) family.

    PTM databases

    iPTMnetiQ9LV10.

    Proteomic databases

    PaxDbiQ9LV10.
    PRIDEiQ9LV10.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT5G62680.1; AT5G62680.1; AT5G62680.
    GeneIDi836389.
    GrameneiAT5G62680.1; AT5G62680.1; AT5G62680.
    KEGGiath:AT5G62680.

    Organism-specific databases

    TAIRiAT5G62680.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237399.
    InParanoidiQ9LV10.
    OMAiYLTITQQ.
    PhylomeDBiQ9LV10.

    Enzyme and pathway databases

    ReactomeiR-ATH-427975. Proton/oligopeptide cotransporters.

    Miscellaneous databases

    PROiQ9LV10.

    Gene expression databases

    GenevisibleiQ9LV10. AT.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.
      DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Nitrate transporters and peptide transporters."
      Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.
      FEBS Lett. 581:2290-2300(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY.
    5. "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance."
      Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z., Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W., Gong J.M.
      Plant Cell 22:1633-1646(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    6. "NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds."
      Nour-Eldin H.H., Andersen T.G., Burow M., Madsen S.R., Jorgensen M.E., Olsen C.E., Dreyer I., Hedrich R., Geiger D., Halkier B.A.
      Nature 488:531-534(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis."
      Andersen T.G., Nour-Eldin H.H., Fuller V.L., Olsen C.E., Burow M., Halkier B.A.
      Plant Cell 25:3133-3145(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    8. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPTR53_ARATH
    AccessioniPrimary (citable) accession number: Q9LV10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: October 1, 2000
    Last modified: February 17, 2016
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.