Q9LUW6 (RH9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DEAD-box ATP-dependent RNA helicase 9
EC=3.6.4.13

Gene names

Name:	RH9
Ordered Locus Names:	At3g22310
ORF Names:	MCB17.17, MCB17.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + H2O = ADP + phosphate.
Domain	The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities	Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to cold Inferred from expression pattern PubMed 18725370. Source: TAIR response to salt stress Inferred from mutant phenotype PubMed 18725370. Source: TAIR response to water deprivation Inferred from mutant phenotype PubMed 18725370. Source: TAIR
Cellular_component	protein complex Inferred from direct assay PubMed 19960362. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from direct assay PubMed 18725370. Source: TAIR RNA binding Inferred from direct assay PubMed 18725370. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	DEAD-box ATP-dependent RNA helicase 9		PRO_0000239151
Regions
	Domain	147 – 321	175	Helicase ATP-binding
	Domain	350 – 494	145	Helicase C-terminal
	Nucleotide binding	160 – 167	8	ATP By similarity
	Motif	116 – 144	29	Q motif
	Motif	269 – 272	4	DEAD box
	Compositional bias	501 – 606	106	Gly-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9LUW6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E85F6FAAF3C3C432	FASTA	610	63,609
	10 20 30 40 50 60 MISTVLRRSI LGTSRRTLAA SVTSINAALF HNLAPAAATV SDLANGATNV KSLPSNSSPF 70 80 90 100 110 120 GVKVRDFHVK SVPSEFRSSI VSSAGFAAQE YAPSYENDGG IGDSESVGSS GGGDGLAIAD 130 140 150 160 170 180 LGISPEIVKA LKGRGIEKLF PIQKAVLEPA MEGRDMIGRA RTGTGKTLAF GIPIIDKIIK 190 200 210 220 230 240 FNAKHGRGKN PQCLVLAPTR ELARQVEKEF RESAPSLDTI CLYGGTPIGQ QMRELNYGID 250 260 270 280 290 300 VAVGTPGRII DLMKRGALNL SEVQFVVLDE ADQMLQVGFA EDVEIILQKL PAKRQSMMFS 310 320 330 340 350 360 ATMPSWIRSL TKKYLNNPLT IDLVGDSDQK LADGITMYSI AADSYGRASI IGPLVKEHGK 370 380 390 400 410 420 GGKCIVFTQT KRDADRLAFG LAKSYKCEAL HGDISQAQRE RTLAGFRDGN FSILVATDVA 430 440 450 460 470 480 ARGLDVPNVD LVIHYELPNN TETFVHRTGR TGRAGKKGSA ILIHGQDQTR AVKMIEKEVG 490 500 510 520 530 540 SRFNELPSIA VERGSASMFE GVGARSGGSF GGGRSGGGGY GSYGSSSGRS GGGSYGGYGG 550 560 570 580 590 600 SSGRSGGGGG SYGGSGGSSS RYSGGSDRSS GFGSFGSGGS SGGFGSDRSS QSSGRSSFGG 610 FGSNDGKRSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The DEAD box RNA helicase family in Arabidopsis thaliana." Aubourg S., Kreis M., Lecharny A. Nucleic Acids Res. 27:628-636(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-610. Strain: cv. Columbia.
[5]	"DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link between quantitative expression, gene structure and evolution of a family of genes." Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M., Lecharny A. Plant Biotechnol. J. 2:401-415(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022215 Genomic DNA. Translation: BAB01768.1. CP002686 Genomic DNA. Translation: AEE76620.1. AY091091 mRNA. Translation: AAM14042.1. AJ010461 mRNA. Translation: CAA09200.1.
IPI	IPI00517476.
PIR	T51341.
RefSeq	NP_188870.1. NM_113129.3.
UniGene	At.25190.
3D structure databases
ProteinModelPortal	Q9LUW6.
SMR	Q9LUW6. Positions 116-472.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9LUW6. 8 interactions.
MINT	MINT-8060674.
STRING	3702.AT3G22310.1-P.
Proteomic databases
PaxDb	Q9LUW6.
PRIDE	Q9LUW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G22310.1; AT3G22310.1; AT3G22310.
GeneID	821800.
KEGG	ath:AT3G22310.
Organism-specific databases
GeneFarm	922. 59.
TAIR	At3g22310.
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000268800.
InParanoid	Q9LUW6.
OMA	VSQGRQV.
PhylomeDB	Q9LUW6.
ProtClustDB	CLSN2684125.
Gene expression databases
Genevestigator	Q9LUW6.
GermOnline	AT3G22310. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00039. DEAD_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RH9_ARATH

Accession

Primary (citable) accession number: Q9LUW6
Secondary accession number(s): Q9ZS11

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program