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Protein

Linoleate 9S-lipoxygenase 5

Gene

LOX5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

9S-lipoxygenase that can use linoleic acid or linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Function as regulators of root development by controlling the emergence of lateral roots.2 Publications

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi542Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi547Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi733Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi737Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi886Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 9S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • lateral root formation Source: UniProtKB
  • lipid oxidation Source: TAIR
  • negative regulation of defense response to insect Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • root development Source: TAIR

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.58 399
ReactomeiR-ATH-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-ATH-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-ATH-2142700 Synthesis of Lipoxins (LX)
R-ATH-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-ATH-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-ATH-6798695 Neutrophil degranulation
UniPathwayiUPA00382

Chemistry databases

SwissLipidsiSLP:000001764

Names & Taxonomyi

Protein namesi
Recommended name:
Linoleate 9S-lipoxygenase 5 (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase 5
Short name:
AtLOX5
Gene namesi
Name:LOX5
Ordered Locus Names:At3g22400
ORF Names:MCB17.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G22400
TAIRilocus:2087837 AT3G22400

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Increment in the number of lateral roots, and moderate increase in the length of the primary root.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003805941 – 886Linoleate 9S-lipoxygenase 5Add BLAST886

Proteomic databases

PaxDbiQ9LUW0
PRIDEiQ9LUW0

PTM databases

iPTMnetiQ9LUW0

Expressioni

Tissue specificityi

Expressed in roots.1 Publication

Developmental stagei

First observed in lateral root primordia (LRP), from the first pericycle divisions. Disappears before root emergence.1 Publication

Gene expression databases

ExpressionAtlasiQ9LUW0 baseline and differential
GenevisibleiQ9LUW0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G22400.1

Structurei

3D structure databases

ProteinModelPortaliQ9LUW0
SMRiQ9LUW0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 180PLATPROSITE-ProRule annotationAdd BLAST146
Domaini183 – 886LipoxygenasePROSITE-ProRule annotationAdd BLAST704

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IH0D Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000230469
InParanoidiQ9LUW0
KOiK15718
OMAiWNEGKIR
OrthoDBiEOG093601GX
PhylomeDBiQ9LUW0

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LUW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHTDIAEIL CVKPKTTKKT KTMEEDVKKT TTMKIEGEVV VMKKNLLDFK
60 70 80 90 100
DVMASLLDRV NELLGRRVSL HLISSHQPDP ANEKRGRLGK AAHLEKWVTK
110 120 130 140 150
IKTSVTAEET AFGVTFDWDE SMGPPAAFVI KNHHHSQFYL KSLTLRGFPD
160 170 180 190 200
GEGGATAIHF ICNSWIYPNH RYRSDRVFFS NKAYLPSETP ELIKELREEE
210 220 230 240 250
LKNLRGNEKG GEFKEWDRVY DYAYYNDLGA PDKGPDSVRP VLGGSPELPY
260 270 280 290 300
PRRGKTGRKS TKSDPKSESR LALLNLNIYV PRDERFSHVK FSDFLAYALK
310 320 330 340 350
SVTQVLVPEI ASVCDKTINE FDSFEDVFHL YDGSIKLANG HTISKLRDVI
360 370 380 390 400
PWEMFRELVR NDGERFLKYP LPDILKESRS AWRTDEEFAR EMLAGLNPVV
410 420 430 440 450
ISRLQEFPPK SCLDSAKYGN QHSSIRTEHI ESNMNGLNVQ EALEQNKLYI
460 470 480 490 500
LDHHDALMPY LTRINSTNTK TYATRTLLLL QADGTLKPLA IELSLPHAQG
510 520 530 540 550
ESYGSVSKVF TPAEKGVEGS VWQLAKAYAA VNDSGYHQLI SHWLQTHAVI
560 570 580 590 600
EPFIIASNRQ LSVVHPIHKL LHPHFRDTMN INALARHVLI NSDGVLERTV
610 620 630 640 650
FPSRYAMEMS SSIYKNWVFT EQALPKDLLK RGVAVEDPNS DNGVKLLIED
660 670 680 690 700
YPFAVDGLEI WSAIKTWVTE YCTFYYNNDK TVQTDTEIQS WWTELRTKGH
710 720 730 740 750
GDKRHESWWP SMQTRDDLIE TCTIIIWIAS ALHAAVNFGQ YPYAGFLPNR
760 770 780 790 800
PTVSRRFMPE PGTDEYAELE EDADVAFLKT ITPQLQTLLG ISIIEILSMH
810 820 830 840 850
STDEIYLGQR DSPNWTADDE PLEAFKRFGK ELELIENNII RRNNDKRFKN
860 870 880
RTGPVNIPYT LLYPNTTDYT REGGITGKGI PNSVSI
Length:886
Mass (Da):101,060
Last modified:July 28, 2009 - v2
Checksum:i0FF118853811E202
GO

Sequence cautioni

The sequence BAB01777 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022215 Genomic DNA Translation: BAB01777.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE76630.1
AJ302043 mRNA Translation: CAC19365.1
RefSeqiNP_188879.2, NM_113137.4
UniGeneiAt.37889

Genome annotation databases

EnsemblPlantsiAT3G22400.1; AT3G22400.1; AT3G22400
GeneIDi821808
GrameneiAT3G22400.1; AT3G22400.1; AT3G22400
KEGGiath:AT3G22400

Similar proteinsi

Entry informationi

Entry nameiLOX5_ARATH
AccessioniPrimary (citable) accession number: Q9LUW0
Secondary accession number(s): Q9FNX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: April 25, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health