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Protein

Linoleate 9S-lipoxygenase 5, chloroplastic

Gene

LOX5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

9S-lipoxygenase that can use linoleic acid or linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Function as regulators of root development by controlling the emergence of lateral roots.2 Publications

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi542 – 5421Iron; catalyticPROSITE-ProRule annotation
Metal bindingi547 – 5471Iron; catalyticPROSITE-ProRule annotation
Metal bindingi733 – 7331Iron; catalyticPROSITE-ProRule annotation
Metal bindingi737 – 7371Iron; catalyticPROSITE-ProRule annotation
Metal bindingi886 – 8861Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • linoleate 9S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • lateral root formation Source: UniProtKB
  • lipid oxidation Source: TAIR
  • negative regulation of defense response to insect Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.58. 399.
ReactomeiR-ATH-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-ATH-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-ATH-2142700. Synthesis of Lipoxins (LX).
R-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-ATH-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Linoleate 9S-lipoxygenase 5, chloroplastic (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase 5
Short name:
AtLOX5
Gene namesi
Name:LOX5
Ordered Locus Names:At3g22400
ORF Names:MCB17.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G22400.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Increment in the number of lateral roots, and moderate increase in the length of the primary root.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 886886Linoleate 9S-lipoxygenase 5, chloroplasticPRO_0000380594Add
BLAST

Proteomic databases

PaxDbiQ9LUW0.
PRIDEiQ9LUW0.

PTM databases

iPTMnetiQ9LUW0.

Expressioni

Tissue specificityi

Expressed in roots.1 Publication

Developmental stagei

First observed in lateral root primordia (LRP), from the first pericycle divisions. Disappears before root emergence.1 Publication

Gene expression databases

GenevisibleiQ9LUW0. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G22400.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LUW0.
SMRiQ9LUW0. Positions 34-886.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 180146PLATPROSITE-ProRule annotationAdd
BLAST
Domaini183 – 886704LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiQ9LUW0.
KOiK15718.
OMAiTISRRPM.
OrthoDBiEOG093601GX.
PhylomeDBiQ9LUW0.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LUW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIHTDIAEIL CVKPKTTKKT KTMEEDVKKT TTMKIEGEVV VMKKNLLDFK
60 70 80 90 100
DVMASLLDRV NELLGRRVSL HLISSHQPDP ANEKRGRLGK AAHLEKWVTK
110 120 130 140 150
IKTSVTAEET AFGVTFDWDE SMGPPAAFVI KNHHHSQFYL KSLTLRGFPD
160 170 180 190 200
GEGGATAIHF ICNSWIYPNH RYRSDRVFFS NKAYLPSETP ELIKELREEE
210 220 230 240 250
LKNLRGNEKG GEFKEWDRVY DYAYYNDLGA PDKGPDSVRP VLGGSPELPY
260 270 280 290 300
PRRGKTGRKS TKSDPKSESR LALLNLNIYV PRDERFSHVK FSDFLAYALK
310 320 330 340 350
SVTQVLVPEI ASVCDKTINE FDSFEDVFHL YDGSIKLANG HTISKLRDVI
360 370 380 390 400
PWEMFRELVR NDGERFLKYP LPDILKESRS AWRTDEEFAR EMLAGLNPVV
410 420 430 440 450
ISRLQEFPPK SCLDSAKYGN QHSSIRTEHI ESNMNGLNVQ EALEQNKLYI
460 470 480 490 500
LDHHDALMPY LTRINSTNTK TYATRTLLLL QADGTLKPLA IELSLPHAQG
510 520 530 540 550
ESYGSVSKVF TPAEKGVEGS VWQLAKAYAA VNDSGYHQLI SHWLQTHAVI
560 570 580 590 600
EPFIIASNRQ LSVVHPIHKL LHPHFRDTMN INALARHVLI NSDGVLERTV
610 620 630 640 650
FPSRYAMEMS SSIYKNWVFT EQALPKDLLK RGVAVEDPNS DNGVKLLIED
660 670 680 690 700
YPFAVDGLEI WSAIKTWVTE YCTFYYNNDK TVQTDTEIQS WWTELRTKGH
710 720 730 740 750
GDKRHESWWP SMQTRDDLIE TCTIIIWIAS ALHAAVNFGQ YPYAGFLPNR
760 770 780 790 800
PTVSRRFMPE PGTDEYAELE EDADVAFLKT ITPQLQTLLG ISIIEILSMH
810 820 830 840 850
STDEIYLGQR DSPNWTADDE PLEAFKRFGK ELELIENNII RRNNDKRFKN
860 870 880
RTGPVNIPYT LLYPNTTDYT REGGITGKGI PNSVSI
Length:886
Mass (Da):101,060
Last modified:July 28, 2009 - v2
Checksum:i0FF118853811E202
GO

Sequence cautioni

The sequence BAB01777 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022215 Genomic DNA. Translation: BAB01777.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76630.1.
AJ302043 mRNA. Translation: CAC19365.1.
RefSeqiNP_188879.2. NM_113137.3.
UniGeneiAt.37889.

Genome annotation databases

EnsemblPlantsiAT3G22400.1; AT3G22400.1; AT3G22400.
GeneIDi821808.
GrameneiAT3G22400.1; AT3G22400.1; AT3G22400.
KEGGiath:AT3G22400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022215 Genomic DNA. Translation: BAB01777.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76630.1.
AJ302043 mRNA. Translation: CAC19365.1.
RefSeqiNP_188879.2. NM_113137.3.
UniGeneiAt.37889.

3D structure databases

ProteinModelPortaliQ9LUW0.
SMRiQ9LUW0. Positions 34-886.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G22400.1.

PTM databases

iPTMnetiQ9LUW0.

Proteomic databases

PaxDbiQ9LUW0.
PRIDEiQ9LUW0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G22400.1; AT3G22400.1; AT3G22400.
GeneIDi821808.
GrameneiAT3G22400.1; AT3G22400.1; AT3G22400.
KEGGiath:AT3G22400.

Organism-specific databases

TAIRiAT3G22400.

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiQ9LUW0.
KOiK15718.
OMAiTISRRPM.
OrthoDBiEOG093601GX.
PhylomeDBiQ9LUW0.

Enzyme and pathway databases

UniPathwayiUPA00382.
BRENDAi1.13.11.58. 399.
ReactomeiR-ATH-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-ATH-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-ATH-2142700. Synthesis of Lipoxins (LX).
R-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-ATH-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.

Miscellaneous databases

PROiQ9LUW0.

Gene expression databases

GenevisibleiQ9LUW0. AT.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOX5_ARATH
AccessioniPrimary (citable) accession number: Q9LUW0
Secondary accession number(s): Q9FNX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 7, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.