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Q9LUT2

- METK4_ARATH

UniProt

Q9LUT2 - METK4_ARATH

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Protein

S-adenosylmethionine synthase 4

Gene

METK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme (By similarity).By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent ions per subunit. Magnesium or cobalt.By similarity
  • K(+)By similarityNote: Binds 1 potassium ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171MagnesiumBy similarity
Metal bindingi43 – 431PotassiumBy similarity
Binding sitei147 – 1471ATPSequence Analysis
Metal bindingi271 – 2711PotassiumBy similarity
Metal bindingi279 – 2791MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi119 – 1246ATPSequence Analysis
Nucleotide bindingi267 – 2748ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methionine adenosyltransferase activity Source: TAIR

GO - Biological processi

  1. lignin biosynthetic process Source: TAIR
  2. methionine metabolic process Source: TAIR
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to cold Source: TAIR
  5. S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT3G17390-MONOMER.
ReactomeiREACT_185282. Sulfur amino acid metabolism.
REACT_256117. Methylation.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase 4 (EC:2.5.1.6)
Short name:
AdoMet synthase 4
Alternative name(s):
Methionine adenosyltransferase 4
Short name:
MAT 4
Gene namesi
Name:METK4
Synonyms:MTO3, SAMS3
Ordered Locus Names:At3g17390
ORF Names:MGD8.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G17390.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cell wall Source: TAIR
  2. cytoplasm Source: UniProtKB-KW
  3. membrane Source: TAIR
  4. nucleolus Source: TAIR
  5. plasma membrane Source: TAIR
  6. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393S-adenosylmethionine synthase 4PRO_0000363004Add
BLAST

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ9LUT2.

Expressioni

Tissue specificityi

Trichomes.1 Publication

Inductioni

Induced by cold.1 Publication

Gene expression databases

ExpressionAtlasiQ9LUT2. baseline and differential.
GenevestigatoriQ9LUT2.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi6336. 3 interactions.
IntActiQ9LUT2. 2 interactions.
MINTiMINT-8064905.
STRINGi3702.AT3G17390.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9LUT2.
SMRiQ9LUT2. Positions 3-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000245710.
InParanoidiQ9LUT2.
KOiK00789.
OMAiDETEESM.
PhylomeDBiQ9LUT2.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LUT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM
60 70 80 90 100
VMVFGEITTK ANVDYEQIVR KTCREIGFVS ADVGLDADNC KVLVNIEQQS
110 120 130 140 150
PDIAQGVHGH LTKKPEEVGA GDQGHMFGYA TDETPELMPL THVLATKLGA
160 170 180 190 200
KLTEVRKNGT CPWLRPDGKT QVTIEYINES GAMVPVRVHT VLISTQHDET
210 220 230 240 250
VTNDEIAADL KEHVIKPVIP EKYLDEKTIF HLNPSGRFVI GGPHGDAGLT
260 270 280 290 300
GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSIVASGLAR
310 320 330 340 350
RVIVQVSYAI GVPEPLSVFV DSYGTGKIPD KEILEIVKES FDFRPGMISI
360 370 380 390
NLDLKRGGNG RFLKTAAYGH FGRDDADFTW EVVKPLKSNK VQA
Length:393
Mass (Da):42,796
Last modified:October 1, 2000 - v1
Checksum:i70034EC2B5E81867
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981D → G in BAF01944. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022216 Genomic DNA. Translation: BAB02743.1.
CP002686 Genomic DNA. Translation: AEE75948.1.
AY037214 mRNA. Translation: AAK59799.1.
AY120708 mRNA. Translation: AAM53266.1.
BT000712 mRNA. Translation: AAN31855.1.
BT002665 mRNA. Translation: AAO11581.1.
AK230129 mRNA. Translation: BAF01944.1.
AY087184 mRNA. Translation: AAM64740.1.
RefSeqiNP_188365.1. NM_112618.3.
UniGeneiAt.5781.
At.70071.

Genome annotation databases

EnsemblPlantsiAT3G17390.1; AT3G17390.1; AT3G17390.
GeneIDi821003.
KEGGiath:AT3G17390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022216 Genomic DNA. Translation: BAB02743.1 .
CP002686 Genomic DNA. Translation: AEE75948.1 .
AY037214 mRNA. Translation: AAK59799.1 .
AY120708 mRNA. Translation: AAM53266.1 .
BT000712 mRNA. Translation: AAN31855.1 .
BT002665 mRNA. Translation: AAO11581.1 .
AK230129 mRNA. Translation: BAF01944.1 .
AY087184 mRNA. Translation: AAM64740.1 .
RefSeqi NP_188365.1. NM_112618.3.
UniGenei At.5781.
At.70071.

3D structure databases

ProteinModelPortali Q9LUT2.
SMRi Q9LUT2. Positions 3-387.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 6336. 3 interactions.
IntActi Q9LUT2. 2 interactions.
MINTi MINT-8064905.
STRINGi 3702.AT3G17390.1-P.

Proteomic databases

PRIDEi Q9LUT2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G17390.1 ; AT3G17390.1 ; AT3G17390 .
GeneIDi 821003.
KEGGi ath:AT3G17390.

Organism-specific databases

TAIRi AT3G17390.

Phylogenomic databases

HOGENOMi HOG000245710.
InParanoidi Q9LUT2.
KOi K00789.
OMAi DETEESM.
PhylomeDBi Q9LUT2.

Enzyme and pathway databases

UniPathwayi UPA00315 ; UER00080 .
BioCyci ARA:AT3G17390-MONOMER.
Reactomei REACT_185282. Sulfur amino acid metabolism.
REACT_256117. Methylation.

Gene expression databases

ExpressionAtlasi Q9LUT2. baseline and differential.
Genevestigatori Q9LUT2.

Family and domain databases

HAMAPi MF_00086. S_AdoMet_synth1.
InterProi IPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view ]
PANTHERi PTHR11964. PTHR11964. 1 hit.
Pfami PF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000497. MAT. 1 hit.
SUPFAMi SSF55973. SSF55973. 3 hits.
TIGRFAMsi TIGR01034. metK. 1 hit.
PROSITEi PS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "A proteomic study of the Arabidopsis nuclear matrix."
    Calikowski T.T., Meulia T., Meier I.
    J. Cell. Biochem. 90:361-378(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification."
    Wienkoop S., Zoeller D., Ebert B., Simon-Rosin U., Fisahn J., Glinski M., Weckwerth W.
    Phytochemistry 65:1641-1649(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
  8. "Proteome analysis of cold stress response in Arabidopsis thaliana using DIGE-technology."
    Amme S., Matros A., Schlesier B., Mock H.-P.
    J. Exp. Bot. 57:1537-1546(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY COLD, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMETK4_ARATH
AccessioniPrimary (citable) accession number: Q9LUT2
Secondary accession number(s): Q0WLR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3