S-adenosylmethionine synthetase 4 - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot Q9LUT2 (METK4_ARATH)

Last modified February 9, 2010. Version 67. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 4
      Short name=AdoMet synthetase 4
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 4
      Short name=MAT 4

Gene names

Name:	METK4
Synonyms:	MTO3, SAMS3
Ordered Locus Names:	At3g17390
ORF Names:	MGD8.23

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H₂O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Ref.5.
Tissue specificity	Trichomes. Ref.6
Induction	Induced by cold. Ref.7
Sequence similarities	Belongs to the AdoMet synthetase family.

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Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from mutant phenotype. Source: TAIR methionine metabolic process Inferred from mutant phenotype. Source: TAIR one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to cold Ref.7 Inferred from expression pattern. Source: TAIR
Cellular component	cell wall Inferred from direct assay. Source: TAIR cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 393

393

S-adenosylmethionine synthetase 4

PRO_0000363004

Regions

Nucleotide binding

119 – 124

ATP Potential

Nucleotide binding

267 – 274

ATP Potential

Sites

Metal binding

Magnesium By similarity

Metal binding

Potassium By similarity

Metal binding

271

Potassium By similarity

Metal binding

279

Magnesium By similarity

Binding site

147

ATP Potential

Experimental info

Sequence conflict

198

D → G in BAF01944. Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9LUT2-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 70034EC2B5E81867
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FASTA

393

42,796

        10         20         30         40         50         60 
MESFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM VMVFGEITTK 

        70         80         90        100        110        120 
ANVDYEQIVR KTCREIGFVS ADVGLDADNC KVLVNIEQQS PDIAQGVHGH LTKKPEEVGA 

       130        140        150        160        170        180 
GDQGHMFGYA TDETPELMPL THVLATKLGA KLTEVRKNGT CPWLRPDGKT QVTIEYINES 

       190        200        210        220        230        240 
GAMVPVRVHT VLISTQHDET VTNDEIAADL KEHVIKPVIP EKYLDEKTIF HLNPSGRFVI 

       250        260        270        280        290        300 
GGPHGDAGLT GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSIVASGLAR 

       310        320        330        340        350        360 
RVIVQVSYAI GVPEPLSVFV DSYGTGKIPD KEILEIVKES FDFRPGMISI NLDLKRGGNG 

       370        380        390 
RFLKTAAYGH FGRDDADFTW EVVKPLKSNK VQA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"A proteomic study of the Arabidopsis nuclear matrix." Calikowski T.T., Meulia T., Meier I. J. Cell. Biochem. 90:361-378(2003) [PubMed: 14505352] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[6]	"Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification." Wienkoop S., Zoeller D., Ebert B., Simon-Rosin U., Fisahn J., Glinski M., Weckwerth W. Phytochemistry 65:1641-1649(2004) [PubMed: 15276459] [Abstract] Cited for: TISSUE SPECIFICITY, IDENTIFICATION BY MASS-SPECTROMETRY.
[7]	"Proteome analysis of cold stress response in Arabidopsis thaliana using DIGE-technology." Amme S., Matros A., Schlesier B., Mock H.-P. J. Exp. Bot. 57:1537-1546(2006) [PubMed: 16574749] [Abstract] Cited for: INDUCTION BY COLD, IDENTIFICATION BY MASS-SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022216 Genomic DNA. Translation: BAB02743.1. AY037214 mRNA. Translation: AAK59799.1. AY120708 mRNA. Translation: AAM53266.1. BT000712 mRNA. Translation: AAN31855.1. BT002665 mRNA. Translation: AAO11581.1. AK230129 mRNA. Translation: BAF01944.1. AY087184 mRNA. Translation: AAM64740.1.
IPI	IPI00536966.
RefSeq	NP_188365.1.
UniGene	At.5781 At.70071 Rra.10437 Rra.550 Rsa.21802
3D structure databases
HSSP	HSSP built from PDB template 1QM4 based on UniProtKB P13444.
SMR	Q9LUT2. Positions 3-387.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9LUT2.
Proteomic databases
PRIDE	Q9LUT2.
Genome annotation databases
GeneID	821003.
GenomeReviews	Gene locus AT3G17390 in contig BA000014_GR.
KEGG	ath:AT3G17390.
NMPDR	fig\|3702.1.peg.13929.
Organism-specific databases
TAIR	At3g17390.
Phylogenomic databases
eggNOG	KOG1506.
HOGENOM	HBG443662.
InParanoid	Q9LUT2.
OMA	VPRKLKY.
PhylomeDB	Q9LUT2.
Gene expression databases
ArrayExpress	Q9LUT2.
Genevestigator	Q9LUT2.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

METK4_ARATH

Accession

Primary (citable) accession number: Q9LUT2
Secondary accession number(s): Q0WLR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents