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Protein

S-adenosylmethionine synthase 4

Gene

METK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate (By similarity). Can utilize magnesium, manganese or cobalt (in vitro) (By similarity).By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate (By similarity).By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase 4 (METK4), S-adenosylmethionine synthase (At4g01850), S-adenosylmethionine synthase 2 (SAM2), S-adenosylmethionine synthase (AXX17_At2g33610), S-adenosylmethionine synthase 3 (METK3), S-adenosylmethionine synthase (AXX17_At3g18350), S-adenosylmethionine synthase (AXX17_At1g01660), S-adenosylmethionine synthase 1 (SAM1), S-adenosylmethionine synthase (AXX17_At4g02310)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumBy similarity1
Binding sitei15ATPBy similarity1
Metal bindingi43PotassiumBy similarity1
Binding sitei56MethionineBy similarity1
Binding sitei99MethionineBy similarity1
Binding sitei246ATPBy similarity1
Binding sitei246Methionine; shared with neighboring subunitBy similarity1
Binding sitei269ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei273ATP; shared with neighboring subunitBy similarity1
Binding sitei277ATP; shared with neighboring subunitBy similarity1
Binding sitei277MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 169ATPBy similarity3
Nucleotide bindingi235 – 238ATPBy similarity4
Nucleotide bindingi252 – 253ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • methionine adenosyltransferase activity Source: TAIR
  • mRNA binding Source: TAIR

GO - Biological processi

  • lignin biosynthetic process Source: TAIR
  • methionine metabolic process Source: TAIR
  • one-carbon metabolic process Source: UniProtKB-KW
  • response to cold Source: TAIR
  • S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processOne-carbon metabolism
LigandATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT3G17390-MONOMER
ReactomeiR-ATH-156581 Methylation
R-ATH-1614635 Sulfur amino acid metabolism
R-ATH-2408508 Metabolism of ingested SeMet, Sec, MeSec into H2Se
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase 4 (EC:2.5.1.6By similarity)
Short name:
AdoMet synthase 4
Alternative name(s):
Methionine adenosyltransferase 4
Short name:
MAT 4
Gene namesi
Name:METK4
Synonyms:MTO3, SAMS3
Ordered Locus Names:At3g17390
ORF Names:MGD8.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G17390
TAIRilocus:2089070 AT3G17390

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003630041 – 393S-adenosylmethionine synthase 4Add BLAST393

Proteomic databases

PaxDbiQ9LUT2
PRIDEiQ9LUT2

PTM databases

iPTMnetiQ9LUT2
SwissPalmiQ9LUT2

Expressioni

Tissue specificityi

Detected in trichomes (at the protein level).1 Publication

Inductioni

Induced by cold.1 Publication

Gene expression databases

ExpressionAtlasiQ9LUT2 baseline and differential
GenevisibleiQ9LUT2 AT

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi6336, 8 interactors
IntActiQ9LUT2, 2 interactors
MINTiQ9LUT2
STRINGi3702.AT3G17390.1

Structurei

3D structure databases

ProteinModelPortaliQ9LUT2
SMRiQ9LUT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506 Eukaryota
COG0192 LUCA
HOGENOMiHOG000245710
InParanoidiQ9LUT2
KOiK00789
OMAiPGHFLFT
OrthoDBiEOG09360BP4
PhylomeDBiQ9LUT2

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LUT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM
60 70 80 90 100
VMVFGEITTK ANVDYEQIVR KTCREIGFVS ADVGLDADNC KVLVNIEQQS
110 120 130 140 150
PDIAQGVHGH LTKKPEEVGA GDQGHMFGYA TDETPELMPL THVLATKLGA
160 170 180 190 200
KLTEVRKNGT CPWLRPDGKT QVTIEYINES GAMVPVRVHT VLISTQHDET
210 220 230 240 250
VTNDEIAADL KEHVIKPVIP EKYLDEKTIF HLNPSGRFVI GGPHGDAGLT
260 270 280 290 300
GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSIVASGLAR
310 320 330 340 350
RVIVQVSYAI GVPEPLSVFV DSYGTGKIPD KEILEIVKES FDFRPGMISI
360 370 380 390
NLDLKRGGNG RFLKTAAYGH FGRDDADFTW EVVKPLKSNK VQA
Length:393
Mass (Da):42,796
Last modified:October 1, 2000 - v1
Checksum:i70034EC2B5E81867
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198D → G in BAF01944 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022216 Genomic DNA Translation: BAB02743.1
CP002686 Genomic DNA Translation: AEE75948.1
AY037214 mRNA Translation: AAK59799.1
AY120708 mRNA Translation: AAM53266.1
BT000712 mRNA Translation: AAN31855.1
BT002665 mRNA Translation: AAO11581.1
AK230129 mRNA Translation: BAF01944.1
AY087184 mRNA Translation: AAM64740.1
RefSeqiNP_188365.1, NM_112618.4
UniGeneiAt.5781
At.70071

Genome annotation databases

EnsemblPlantsiAT3G17390.1; AT3G17390.1; AT3G17390
GeneIDi821003
GrameneiAT3G17390.1; AT3G17390.1; AT3G17390
KEGGiath:AT3G17390

Similar proteinsi

Entry informationi

Entry nameiMETK4_ARATH
AccessioniPrimary (citable) accession number: Q9LUT2
Secondary accession number(s): Q0WLR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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