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Protein

S-adenosylmethionine synthase 4

Gene

METK4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.By similarity

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarity, Mg2+By similarity, Co2+By similarityNote: Binds 2 divalent ions per subunit. The metal ions interact primarily with the substrate (By similarity). Can utilize magnesium, manganese or cobalt (in vitro) (By similarity).By similarity
  • K+By similarityNote: Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate (By similarity).By similarity

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase 4 (METK4), S-adenosylmethionine synthase (At4g01850), S-adenosylmethionine synthase 2 (SAM2), S-adenosylmethionine synthase (AXX17_At2g33610), S-adenosylmethionine synthase 3 (METK3), S-adenosylmethionine synthase (AXX17_At3g18350), S-adenosylmethionine synthase (AXX17_At1g01660), S-adenosylmethionine synthase 1 (SAM1), S-adenosylmethionine synthase (AXX17_At4g02310)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumBy similarity1
Binding sitei15ATPBy similarity1
Metal bindingi43PotassiumBy similarity1
Binding sitei56MethionineBy similarity1
Binding sitei99MethionineBy similarity1
Binding sitei246ATPBy similarity1
Binding sitei246Methionine; shared with neighboring subunitBy similarity1
Binding sitei269ATP; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei273ATP; shared with neighboring subunitBy similarity1
Binding sitei277ATP; shared with neighboring subunitBy similarity1
Binding sitei277MethionineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi167 – 169ATPBy similarity3
Nucleotide bindingi235 – 238ATPBy similarity4
Nucleotide bindingi252 – 253ATPBy similarity2

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • methionine adenosyltransferase activity Source: TAIR

GO - Biological processi

  • lignin biosynthetic process Source: TAIR
  • methionine metabolic process Source: TAIR
  • one-carbon metabolic process Source: UniProtKB-KW
  • response to cold Source: TAIR
  • S-adenosylmethionine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Cobalt, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT3G17390-MONOMER.
ReactomeiR-ATH-156581. Methylation.
R-ATH-1614635. Sulfur amino acid metabolism.
R-ATH-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.
UniPathwayiUPA00315; UER00080.

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthase 4 (EC:2.5.1.6By similarity)
Short name:
AdoMet synthase 4
Alternative name(s):
Methionine adenosyltransferase 4
Short name:
MAT 4
Gene namesi
Name:METK4
Synonyms:MTO3, SAMS3
Ordered Locus Names:At3g17390
ORF Names:MGD8.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G17390.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • cytosol Source: GO_Central
  • membrane Source: TAIR
  • nucleolus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003630041 – 393S-adenosylmethionine synthase 4Add BLAST393

Proteomic databases

PaxDbiQ9LUT2.
PRIDEiQ9LUT2.

PTM databases

iPTMnetiQ9LUT2.
SwissPalmiQ9LUT2.

Expressioni

Tissue specificityi

Trichomes.1 Publication

Inductioni

Induced by cold.1 Publication

Gene expression databases

GenevisibleiQ9LUT2. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi6336. 8 interactors.
IntActiQ9LUT2. 2 interactors.
MINTiMINT-8064905.
STRINGi3702.AT3G17390.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LUT2.
SMRiQ9LUT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AdoMet synthase family.Curated

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
HOGENOMiHOG000245710.
InParanoidiQ9LUT2.
KOiK00789.
OMAiSVQHDEC.
OrthoDBiEOG09360BP4.
PhylomeDBiQ9LUT2.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LUT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFLFTSES VNEGHPDKLC DQISDAILDA CLEQDPESKV ACETCTKTNM
60 70 80 90 100
VMVFGEITTK ANVDYEQIVR KTCREIGFVS ADVGLDADNC KVLVNIEQQS
110 120 130 140 150
PDIAQGVHGH LTKKPEEVGA GDQGHMFGYA TDETPELMPL THVLATKLGA
160 170 180 190 200
KLTEVRKNGT CPWLRPDGKT QVTIEYINES GAMVPVRVHT VLISTQHDET
210 220 230 240 250
VTNDEIAADL KEHVIKPVIP EKYLDEKTIF HLNPSGRFVI GGPHGDAGLT
260 270 280 290 300
GRKIIIDTYG GWGAHGGGAF SGKDPTKVDR SGAYIVRQAA KSIVASGLAR
310 320 330 340 350
RVIVQVSYAI GVPEPLSVFV DSYGTGKIPD KEILEIVKES FDFRPGMISI
360 370 380 390
NLDLKRGGNG RFLKTAAYGH FGRDDADFTW EVVKPLKSNK VQA
Length:393
Mass (Da):42,796
Last modified:October 1, 2000 - v1
Checksum:i70034EC2B5E81867
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198D → G in BAF01944 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022216 Genomic DNA. Translation: BAB02743.1.
CP002686 Genomic DNA. Translation: AEE75948.1.
AY037214 mRNA. Translation: AAK59799.1.
AY120708 mRNA. Translation: AAM53266.1.
BT000712 mRNA. Translation: AAN31855.1.
BT002665 mRNA. Translation: AAO11581.1.
AK230129 mRNA. Translation: BAF01944.1.
AY087184 mRNA. Translation: AAM64740.1.
RefSeqiNP_188365.1. NM_112618.4.
UniGeneiAt.5781.
At.70071.

Genome annotation databases

EnsemblPlantsiAT3G17390.1; AT3G17390.1; AT3G17390.
GeneIDi821003.
GrameneiAT3G17390.1; AT3G17390.1; AT3G17390.
KEGGiath:AT3G17390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022216 Genomic DNA. Translation: BAB02743.1.
CP002686 Genomic DNA. Translation: AEE75948.1.
AY037214 mRNA. Translation: AAK59799.1.
AY120708 mRNA. Translation: AAM53266.1.
BT000712 mRNA. Translation: AAN31855.1.
BT002665 mRNA. Translation: AAO11581.1.
AK230129 mRNA. Translation: BAF01944.1.
AY087184 mRNA. Translation: AAM64740.1.
RefSeqiNP_188365.1. NM_112618.4.
UniGeneiAt.5781.
At.70071.

3D structure databases

ProteinModelPortaliQ9LUT2.
SMRiQ9LUT2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6336. 8 interactors.
IntActiQ9LUT2. 2 interactors.
MINTiMINT-8064905.
STRINGi3702.AT3G17390.1.

PTM databases

iPTMnetiQ9LUT2.
SwissPalmiQ9LUT2.

Proteomic databases

PaxDbiQ9LUT2.
PRIDEiQ9LUT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G17390.1; AT3G17390.1; AT3G17390.
GeneIDi821003.
GrameneiAT3G17390.1; AT3G17390.1; AT3G17390.
KEGGiath:AT3G17390.

Organism-specific databases

TAIRiAT3G17390.

Phylogenomic databases

eggNOGiKOG1506. Eukaryota.
COG0192. LUCA.
HOGENOMiHOG000245710.
InParanoidiQ9LUT2.
KOiK00789.
OMAiSVQHDEC.
OrthoDBiEOG09360BP4.
PhylomeDBiQ9LUT2.

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080.
BioCyciARA:AT3G17390-MONOMER.
ReactomeiR-ATH-156581. Methylation.
R-ATH-1614635. Sulfur amino acid metabolism.
R-ATH-2408508. Metabolism of ingested SeMet, Sec, MeSec into H2Se.

Miscellaneous databases

PROiQ9LUT2.

Gene expression databases

GenevisibleiQ9LUT2. AT.

Family and domain databases

HAMAPiMF_00086. S_AdoMet_synth1. 1 hit.
InterProiIPR022631. ADOMET_SYNTHASE_CS.
IPR022630. S-AdoMet_synt_C.
IPR022629. S-AdoMet_synt_central.
IPR022628. S-AdoMet_synt_N.
IPR002133. S-AdoMet_synthetase.
IPR022636. S-AdoMet_synthetase_sfam.
[Graphical view]
PANTHERiPTHR11964. PTHR11964. 1 hit.
PfamiPF02773. S-AdoMet_synt_C. 1 hit.
PF02772. S-AdoMet_synt_M. 1 hit.
PF00438. S-AdoMet_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000497. MAT. 1 hit.
SUPFAMiSSF55973. SSF55973. 3 hits.
TIGRFAMsiTIGR01034. metK. 1 hit.
PROSITEiPS00376. ADOMET_SYNTHASE_1. 1 hit.
PS00377. ADOMET_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETK4_ARATH
AccessioniPrimary (citable) accession number: Q9LUT2
Secondary accession number(s): Q0WLR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.