ID   P2C40_ARATH             Reviewed;         493 AA.
AC   Q9LUS8; Q8LCU0;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 131.
DE   RecName: Full=Probable protein phosphatase 2C 40;
DE            Short=AtPP2C40;
DE            EC=3.1.3.16;
DE   AltName: Full=Protein phosphatase 2C homolog 1;
GN   OrderedLocusNames=At3g16560; ORFNames=MDC8.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit. {ECO:0000250};
CC   -!- INTERACTION:
CC       Q9LUS8; Q17TI5: BRX; NbExp=3; IntAct=EBI-25519200, EBI-4426649;
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
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DR   EMBL; AB022217; BAB02747.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75836.1; -; Genomic_DNA.
DR   EMBL; CP002686; ANM63918.1; -; Genomic_DNA.
DR   EMBL; CP002686; ANM63920.1; -; Genomic_DNA.
DR   EMBL; AY136458; AAM97123.1; -; mRNA.
DR   EMBL; BT008864; AAP68303.1; -; mRNA.
DR   EMBL; AY086406; AAM64473.1; -; mRNA.
DR   RefSeq; NP_001325978.1; NM_001338238.1.
DR   RefSeq; NP_001325980.1; NM_001338240.1.
DR   RefSeq; NP_566554.1; NM_112529.3.
DR   AlphaFoldDB; Q9LUS8; -.
DR   SMR; Q9LUS8; -.
DR   BioGRID; 6239; 1.
DR   IntAct; Q9LUS8; 2.
DR   STRING; 3702.Q9LUS8; -.
DR   iPTMnet; Q9LUS8; -.
DR   PaxDb; 3702-AT3G16560-1; -.
DR   ProteomicsDB; 248798; -.
DR   EnsemblPlants; AT3G16560.1; AT3G16560.1; AT3G16560.
DR   EnsemblPlants; AT3G16560.2; AT3G16560.2; AT3G16560.
DR   EnsemblPlants; AT3G16560.4; AT3G16560.4; AT3G16560.
DR   GeneID; 820905; -.
DR   Gramene; AT3G16560.1; AT3G16560.1; AT3G16560.
DR   Gramene; AT3G16560.2; AT3G16560.2; AT3G16560.
DR   Gramene; AT3G16560.4; AT3G16560.4; AT3G16560.
DR   KEGG; ath:AT3G16560; -.
DR   Araport; AT3G16560; -.
DR   TAIR; AT3G16560; -.
DR   eggNOG; KOG0700; Eukaryota.
DR   HOGENOM; CLU_013173_12_3_1; -.
DR   InParanoid; Q9LUS8; -.
DR   OMA; GIDNCHG; -.
DR   OrthoDB; 999128at2759; -.
DR   PhylomeDB; Q9LUS8; -.
DR   PRO; PR:Q9LUS8; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LUS8; baseline and differential.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   CDD; cd00143; PP2Cc; 1.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR015655; PP2C.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   PANTHER; PTHR13832:SF550; PROTEIN PHOSPHATASE 1L; 1.
DR   PANTHER; PTHR13832; PROTEIN PHOSPHATASE 2C; 1.
DR   Pfam; PF00481; PP2C; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q9LUS8; AT.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW   Reference proteome.
FT   CHAIN           1..493
FT                   /note="Probable protein phosphatase 2C 40"
FT                   /id="PRO_0000301257"
FT   DOMAIN          145..480
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   BINDING         180
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         180
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         181
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         408
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         471
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        372
FT                   /note="G -> E (in Ref. 4; AAM64473)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   493 AA;  53614 MW;  613ED80B06B8C844 CRC64;
     MQEGTDPYGE IEISFGYQCN NKKIGIPEDK IADGREVLGG FRLQKTSSFS CLSGAALSGN
     PTLANTNICN GVIGSEILPS LDSPKSFRKV PSSPALSKLD ILSPSLHGSM VSLSCSSSTS
     PSPPEPESCY LTSMSSPSSV NEGFLLSAME VQVAGGAAGE DRVQAVCSEE NGWLFCAIYD
     GFNGRDAADF LACTLYESIV FHLQLLDRQM KQTKSDDDGE KLELLSNISN VDYSSTDLFR
     QGVLDCLNRA LFQAETDFLR MVEQEMEERP DLVSVGSCVL VTLLVGKDLY VLNLGDSRAV
     LATYNGNKKL QAVQLTEDHT VDNEVEEARL LSEHLDDPKI VIGGKIKGKL KVTRALGVGY
     LKKEKLNDAL MGILRVRNLL SPPYVSVEPS MRVHKITESD HFVIVASDGL FDFFSNEEAI
     GLVHSFVSSN PSGDPAKFLL ERLVAKAAAR AGFTLEELTN VPAGRRRRYH DDVTIMVITL
     GTDQRTSKAS TFV
//