Q9LUS8 (P2C40_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 40
Short name=AtPP2C40
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C homolog 1

Gene names

Ordered Locus Names:	At3g16560
ORF Names:	MDC8.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Probable protein phosphatase 2C 40		PRO_0000301257
Regions
	Domain	139 – 452	314	PP2C-like
	Compositional bias	80 – 139	60	Ser-rich
	Compositional bias	465 – 468	4	Poly-Arg
Sites
	Metal binding	180	1	Manganese 1 By similarity
	Metal binding	180	1	Manganese 2 By similarity
	Metal binding	181	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	408	1	Manganese 2 By similarity
	Metal binding	471	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	372	1	G → E in AAM64473. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9LUS8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 613ED80B06B8C844	FASTA	493	53,614
	10 20 30 40 50 60 MQEGTDPYGE IEISFGYQCN NKKIGIPEDK IADGREVLGG FRLQKTSSFS CLSGAALSGN 70 80 90 100 110 120 PTLANTNICN GVIGSEILPS LDSPKSFRKV PSSPALSKLD ILSPSLHGSM VSLSCSSSTS 130 140 150 160 170 180 PSPPEPESCY LTSMSSPSSV NEGFLLSAME VQVAGGAAGE DRVQAVCSEE NGWLFCAIYD 190 200 210 220 230 240 GFNGRDAADF LACTLYESIV FHLQLLDRQM KQTKSDDDGE KLELLSNISN VDYSSTDLFR 250 260 270 280 290 300 QGVLDCLNRA LFQAETDFLR MVEQEMEERP DLVSVGSCVL VTLLVGKDLY VLNLGDSRAV 310 320 330 340 350 360 LATYNGNKKL QAVQLTEDHT VDNEVEEARL LSEHLDDPKI VIGGKIKGKL KVTRALGVGY 370 380 390 400 410 420 LKKEKLNDAL MGILRVRNLL SPPYVSVEPS MRVHKITESD HFVIVASDGL FDFFSNEEAI 430 440 450 460 470 480 GLVHSFVSSN PSGDPAKFLL ERLVAKAAAR AGFTLEELTN VPAGRRRRYH DDVTIMVITL 490 GTDQRTSKAS TFV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022217 Genomic DNA. Translation: BAB02747.1. CP002686 Genomic DNA. Translation: AEE75836.1. AY136458 mRNA. Translation: AAM97123.1. BT008864 mRNA. Translation: AAP68303.1. AY086406 mRNA. Translation: AAM64473.1.
IPI	IPI00530450.
RefSeq	NP_566554.1. NM_112529.2.
UniGene	At.38878.
3D structure databases
ProteinModelPortal	Q9LUS8.
SMR	Q9LUS8. Positions 131-482.
ModBase	Search...
Proteomic databases
PRIDE	Q9LUS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G16560.1; AT3G16560.1; AT3G16560.
GeneID	820905.
KEGG	ath:AT3G16560.
Organism-specific databases
TAIR	At3g16560.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000240776.
InParanoid	Q9LUS8.
OMA	ICNGVIG.
PhylomeDB	Q9LUS8.
ProtClustDB	CLSN2688452.
Gene expression databases
Genevestigator	Q9LUS8.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C40_ARATH

Accession

Primary (citable) accession number: Q9LUS8
Secondary accession number(s): Q8LCU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program