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Q9LUQ5

- UBC25_ARATH

UniProt

Q9LUQ5 - UBC25_ARATH

Protein

Probable ubiquitin-conjugating enzyme E2 25

Gene

UBC25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei418 – 4181Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G15355-MONOMER.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ubiquitin-conjugating enzyme E2 25 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 25
    Gene namesi
    Name:UBC25
    Ordered Locus Names:At3g15355
    ORF Names:MJK13.1
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G15355.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 609609Probable ubiquitin-conjugating enzyme E2 25PRO_0000345190Add
    BLAST

    Proteomic databases

    PaxDbiQ9LUQ5.
    PRIDEiQ9LUQ5.

    Expressioni

    Tissue specificityi

    Expressed in seeds, pistils, siliques, hypocotyls and leaves.1 Publication

    Gene expression databases

    GenevestigatoriQ9LUQ5.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G15355.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9LUQ5.
    SMRiQ9LUQ5. Positions 303-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi577 – 5826Poly-Glu

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000237682.
    InParanoidiQ9LUQ5.
    KOiK10581.
    OMAiHIRERDR.
    PhylomeDBiQ9LUQ5.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9LUQ5-1 [UniParc]FASTAAdd to Basket

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    MEPNVVEIAT PPAASCSRIR TPTKAETPEV IDVEEYDLQN GGVPNGNNVD    50
    YKNKGKAIDF DSMSYGDYGE EDEYAVGSPG DDYGYPESSP LSNSLLDPES 100
    LIYEDDENYS EQYDFEMEAE PDNYSMYQDL FDGKDIPTGV EVSMDWFPNS 150
    ADKESASSSK SSHANNGNNS SKKATKASGI HSQFSSDMET PVAQPWNALP 200
    HKAEGVIPNS AYALPQNSKA FQPPYAVHYS ALKTAFSNYL QPQTPDTVLG 250
    EAPAPAAGSS GLLPPNTPGF KSNAARFKEE PPILPPDDSR VKRNMEDYLG 300
    LYLFFKRFDI VEDFSDHHYA SKGTTSKQHS KDWAKRIQDE WRILEKDLPE 350
    MIFVRAYESR MDLLRAVIIG AQGTPYHDGL FFFDIFFPDT YPSTPPIVHY 400
    HSGGLRINPN LYNCGKVCLS LLGTWSGNQR EKWIPNTSTM LQVLVSIQGL 450
    ILNQKPYFNE PGYERSAGSA HGESTSKAYS ENTFILSLKT MVYTMRRPPK 500
    YFEDFAYGHF FSCAHDVLKA CNAYRNGATP GYLVKGAPDV EENSAGMSSL 550
    KFRTDVATFV ETVLLKEFIL LGVLGLEPEE EEKTPETIIV AESSKCTRSR 600
    SKRDRVSSS 609
    Length:609
    Mass (Da):67,769
    Last modified:October 1, 2000 - v1
    Checksum:i94A2C2E839DED309
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB022218, AP000413 Genomic DNA. Translation: BAB02364.1.
    AC024081 Genomic DNA. Translation: AAF35401.1.
    CP002686 Genomic DNA. Translation: AEE75657.1.
    AF361806 mRNA. Translation: AAK32819.1.
    AY059155 mRNA. Translation: AAL15380.1.
    DQ027038 mRNA. Translation: AAY44864.1.
    RefSeqiNP_188154.1. NM_112402.2.
    UniGeneiAt.27040.
    At.67741.

    Genome annotation databases

    EnsemblPlantsiAT3G15355.1; AT3G15355.1; AT3G15355.
    GeneIDi820772.
    KEGGiath:AT3G15355.

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB022218 , AP000413 Genomic DNA. Translation: BAB02364.1 .
    AC024081 Genomic DNA. Translation: AAF35401.1 .
    CP002686 Genomic DNA. Translation: AEE75657.1 .
    AF361806 mRNA. Translation: AAK32819.1 .
    AY059155 mRNA. Translation: AAL15380.1 .
    DQ027038 mRNA. Translation: AAY44864.1 .
    RefSeqi NP_188154.1. NM_112402.2.
    UniGenei At.27040.
    At.67741.

    3D structure databases

    ProteinModelPortali Q9LUQ5.
    SMRi Q9LUQ5. Positions 303-563.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G15355.1-P.

    Proteomic databases

    PaxDbi Q9LUQ5.
    PRIDEi Q9LUQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G15355.1 ; AT3G15355.1 ; AT3G15355 .
    GeneIDi 820772.
    KEGGi ath:AT3G15355.

    Organism-specific databases

    TAIRi AT3G15355.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000237682.
    InParanoidi Q9LUQ5.
    KOi K10581.
    OMAi HIRERDR.
    PhylomeDBi Q9LUQ5.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT3G15355-MONOMER.

    Gene expression databases

    Genevestigatori Q9LUQ5.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 351-609, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiUBC25_ARATH
    AccessioniPrimary (citable) accession number: Q9LUQ5
    Secondary accession number(s): Q4TYY6, Q9M7X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3