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Q9LUQ5 (UBC25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ubiquitin-conjugating enzyme E2 25

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 25
Gene names
Name:UBC25
Ordered Locus Names:At3g15355
ORF Names:MJK13.1
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Tissue specificity

Expressed in seeds, pistils, siliques, hypocotyls and leaves. Ref.6

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 609609Probable ubiquitin-conjugating enzyme E2 25
PRO_0000345190

Regions

Compositional bias577 – 5826Poly-Glu

Sites

Active site4181Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9LUQ5 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 94A2C2E839DED309

FASTA60967,769
        10         20         30         40         50         60 
MEPNVVEIAT PPAASCSRIR TPTKAETPEV IDVEEYDLQN GGVPNGNNVD YKNKGKAIDF 

        70         80         90        100        110        120 
DSMSYGDYGE EDEYAVGSPG DDYGYPESSP LSNSLLDPES LIYEDDENYS EQYDFEMEAE 

       130        140        150        160        170        180 
PDNYSMYQDL FDGKDIPTGV EVSMDWFPNS ADKESASSSK SSHANNGNNS SKKATKASGI 

       190        200        210        220        230        240 
HSQFSSDMET PVAQPWNALP HKAEGVIPNS AYALPQNSKA FQPPYAVHYS ALKTAFSNYL 

       250        260        270        280        290        300 
QPQTPDTVLG EAPAPAAGSS GLLPPNTPGF KSNAARFKEE PPILPPDDSR VKRNMEDYLG 

       310        320        330        340        350        360 
LYLFFKRFDI VEDFSDHHYA SKGTTSKQHS KDWAKRIQDE WRILEKDLPE MIFVRAYESR 

       370        380        390        400        410        420 
MDLLRAVIIG AQGTPYHDGL FFFDIFFPDT YPSTPPIVHY HSGGLRINPN LYNCGKVCLS 

       430        440        450        460        470        480 
LLGTWSGNQR EKWIPNTSTM LQVLVSIQGL ILNQKPYFNE PGYERSAGSA HGESTSKAYS 

       490        500        510        520        530        540 
ENTFILSLKT MVYTMRRPPK YFEDFAYGHF FSCAHDVLKA CNAYRNGATP GYLVKGAPDV 

       550        560        570        580        590        600 
EENSAGMSSL KFRTDVATFV ETVLLKEFIL LGVLGLEPEE EEKTPETIIV AESSKCTRSR 


SKRDRVSSS 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 351-609, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB022218, AP000413 Genomic DNA. Translation: BAB02364.1.
AC024081 Genomic DNA. Translation: AAF35401.1.
CP002686 Genomic DNA. Translation: AEE75657.1.
AF361806 mRNA. Translation: AAK32819.1.
AY059155 mRNA. Translation: AAL15380.1.
DQ027038 mRNA. Translation: AAY44864.1.
RefSeqNP_188154.1. NM_112402.2.
UniGeneAt.27040.
At.67741.

3D structure databases

ProteinModelPortalQ9LUQ5.
SMRQ9LUQ5. Positions 303-563.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G15355.1-P.

Proteomic databases

PaxDbQ9LUQ5.
PRIDEQ9LUQ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G15355.1; AT3G15355.1; AT3G15355.
GeneID820772.
KEGGath:AT3G15355.

Organism-specific databases

TAIRAT3G15355.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000237682.
InParanoidQ9LUQ5.
KOK10581.
OMAHIRERDR.
PhylomeDBQ9LUQ5.

Enzyme and pathway databases

BioCycARA:AT3G15355-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorQ9LUQ5.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC25_ARATH
AccessionPrimary (citable) accession number: Q9LUQ5
Secondary accession number(s): Q4TYY6, Q9M7X2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names