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Protein

1-phosphatidylinositol-3-phosphate 5-kinase FAB1B

Gene

FAB1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate (By similarity). Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.By similarity3 Publications

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate.

Cofactori

Mg2+By similarity, Mn2+By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri39 – 10567FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • 1-phosphatidylinositol-3-phosphate 5-kinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • endomembrane system organization Source: TAIR
  • phosphatidylinositol phosphorylation Source: GOC
  • pollen development Source: TAIR
  • stomatal closure Source: TAIR
  • vacuole organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G14270-MONOMER.
ReactomeiR-ATH-1660514. Synthesis of PIPs at the Golgi membrane.
R-ATH-1660516. Synthesis of PIPs at the early endosome membrane.
R-ATH-1660517. Synthesis of PIPs at the late endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol-3-phosphate 5-kinase FAB1B (EC:2.7.1.150)
Short name:
Phosphatidylinositol 3-phosphate 5-kinase
Alternative name(s):
FYVE finger-containing phosphoinositide kinase
PIKfyve
Phosphatidylinositol 3-phosphate 5-kinase type III
Short name:
PIPkin-III
Short name:
Type III PIP kinase
Protein FORMS APLOID AND BINUCLEATE CELLS 1B
Gene namesi
Name:FAB1B
Ordered Locus Names:At3g14270
ORF Names:MLN21.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G14270.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: TAIR
  • endosome membrane Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Leaf-curling phenotype. Root growth inhibition, root gravitropic response, and hyposensitivity to exogenous auxin. Fab1a and fab1b double mutant displays an abnormal vacuolar phenotype late in pollen development leading to inviable pollen (PubMed:19846542).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179117911-phosphatidylinositol-3-phosphate 5-kinase FAB1BPRO_0000421871Add
BLAST

Proteomic databases

PaxDbiQ9LUM0.
PRIDEiQ9LUM0.

PTM databases

iPTMnetiQ9LUM0.

Expressioni

Tissue specificityi

Ubiquitous with highest expression levels in the root hair zone, pollen, and stamens.1 Publication

Gene expression databases

GenevisibleiQ9LUM0. AT.

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G14270.1.

Structurei

3D structure databases

ProteinModelPortaliQ9LUM0.
SMRiQ9LUM0. Positions 36-107, 392-665, 1494-1759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1433 – 1758326PIPKPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1077 – 111135Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 PIPK domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri39 – 10567FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0230. Eukaryota.
COG0459. LUCA.
COG5253. LUCA.
HOGENOMiHOG000241144.
InParanoidiQ9LUM0.
KOiK00921.
OMAiRDECEAS.
PhylomeDBiQ9LUM0.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
PF01363. FYVE. 1 hit.
PF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00330. PIPKc. 1 hit.
[Graphical view]
SUPFAMiSSF52029. SSF52029. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51455. PIPK. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LUM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTRDSNNRT FSEIVGLIKS WLPWRSEPAT VSRDFWMPDQ SCRVCYECDC
60 70 80 90 100
QFTLINRRHH CRHCGRVFCG KCTANSIPFA PSDLRTPRED WERIRVCNYC
110 120 130 140 150
FRQWEQGDGG PHVSNITELS TSPSETSLLS SKTSTTANSS SFALGSMPGL
160 170 180 190 200
IGLNQRVHHG SDVSLHGVSS METSVTKQGK ETSRRSSFIA TDVEDPSRFA
210 220 230 240 250
LNSIRSDDEY DEYGAYQTDI ETSHSPRANE YYGPMEYNGM GIDDVPCKHL
260 270 280 290 300
GGETADQKSL SGSPLIHQCL ESLIREGSEQ FQKKSEHDGR DECEASSPAD
310 320 330 340 350
ISDDQVVEPV DFENNGLLWV PPEPENEEDE RESALFDEED NEGDASGEWG
360 370 380 390 400
YLRPSTSFGS GEYRGEDRTT EEHKKAMKNV VDGHFRALLA QLLQVENISV
410 420 430 440 450
SDEEGKESWL EIITSLSWEA ANLLKPDMSK SGGMDPGGYV KVKCLASGFR
460 470 480 490 500
HDSMVVKGVV CKKNVVNRRM STKIEKARLL ILGGGLEYQR VSNQLSSFDT
510 520 530 540 550
LLQQEKDHLK MAVAKIHAER PNILLVEKSV SRFAQEYLLA KDISLVLNIK
560 570 580 590 600
RPLLDRIARC TGAQIIPSVD HLSSQKLGYC ENFRVDRYPE EHGSTGQVGK
610 620 630 640 650
KVVKTLMYFE HCPKPLGFTI LLRGANEDEL KKVKHVVQYG VFAAYHLALE
660 670 680 690 700
TSFLADEGAS PELPLNSPIT VALPDKSTSI ERSISTVPGF TVSTYEKSPT
710 720 730 740 750
MLSCAEPQRA NSVPVSELLS TTTNLSIQKD IPPIPYGSGW QAREINPSFV
760 770 780 790 800
FSRHNISLNL PDRVIESRNS DLSGRSVPVD TPADKSNPIV VADETTNNSL
810 820 830 840 850
HLSGQGFVRK SSQIGTSIMV ENQDNGSELT IAQQQNNEKP KETQSQKEEF
860 870 880 890 900
PPSPSDHQSI LVSLSSRSVW KGTVCERSHL FRIKYYGSFD KPLGRFLRDH
910 920 930 940 950
LFDQSYRCRS CEMPSEAHVH CYTHRQGSLT ISVKKLQDYL LPGEKEGKIW
960 970 980 990 1000
MWHRCLRCPR LNGFPPATLR VVMSDAAWGL SFGKFLELSF SNHAAASRVA
1010 1020 1030 1040 1050
CCGHSLHRDC LRFYGFGNMV ACFRYATIDV HSVYLPPSIL SFNYENQDWI
1060 1070 1080 1090 1100
QRETDEVIER AELLFSEVLN AISQIAEKGF RRRIGELEEV LQKEKAEFEE
1110 1120 1130 1140 1150
NMQKILHREV NEGQPLVDIL ELYRIHRQLL FQSYMWDHRL INASTLHKLE
1160 1170 1180 1190 1200
NSDDTKREEN EKPPLAKSQT LPEMNAGTNS LLTGSEVNLN PDGDSTGDTG
1210 1220 1230 1240 1250
SLNNVQKEAD TNSDLYQEKD DGGEVSPSKT LPDTSYPLEN KVDVRRTQSD
1260 1270 1280 1290 1300
GQIVMKNLSA TLDAAWIGER QTSVEIPTNN KVSLPPSTMS NSSTFPPISE
1310 1320 1330 1340 1350
GLMPIDLPEQ QNEFKVAYPV SPALPSKNYE NSEDSVSWLS VPFLNFYRSI
1360 1370 1380 1390 1400
NKNFLLSSQK LDTFGEHSPI YISSFREAEL QGGPRLLLPV GLNDIVVPVY
1410 1420 1430 1440 1450
DDEPTSMIAY ALMSPEYQRQ TSAEGESLVS YPSELNIPRP VDDTIFDPSR
1460 1470 1480 1490 1500
SNGSVDESIL SISSSRSTSL LDPLSYTKAL HARVSYGEDG TLGKVKYTVT
1510 1520 1530 1540 1550
CYYAKRFEAL RGICLPSELE YIRSLSRCKK WGAQGGKSNV FFAKTLDDRF
1560 1570 1580 1590 1600
IIKQVTKTEL ESFIKFAPAY FKYLSESIST KSPTCLAKIL GIYQVATKQL
1610 1620 1630 1640 1650
KSGKETKMDV LIMENLLFGR TVKRLYDLKG SSRARYNPDS SGSNKVLLDQ
1660 1670 1680 1690 1700
NLIEAMPTSP IFVGNKAKRL LERAVWNDTA FLALGDVMDY SLLVGVDEEK
1710 1720 1730 1740 1750
NELVLGIIDF LRQYTWDKHL ESWVKFTGIL GGPKNEAPTV ISPKQYKRRF
1760 1770 1780 1790
RKAMTTYFLM VPDQWSPPNV VANNSKSDQP EETSQAGTQA E
Length:1,791
Mass (Da):200,831
Last modified:October 1, 2000 - v1
Checksum:i859835D68E56039F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022220 Genomic DNA. Translation: BAB01033.1.
CP002686 Genomic DNA. Translation: AEE75496.1.
AK228922 mRNA. Translation: BAF00811.1.
RefSeqiNP_188044.1. NM_112285.3.
UniGeneiAt.23226.
At.71114.

Genome annotation databases

EnsemblPlantsiAT3G14270.1; AT3G14270.1; AT3G14270.
GeneIDi820647.
GrameneiAT3G14270.1; AT3G14270.1; AT3G14270.
KEGGiath:AT3G14270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022220 Genomic DNA. Translation: BAB01033.1.
CP002686 Genomic DNA. Translation: AEE75496.1.
AK228922 mRNA. Translation: BAF00811.1.
RefSeqiNP_188044.1. NM_112285.3.
UniGeneiAt.23226.
At.71114.

3D structure databases

ProteinModelPortaliQ9LUM0.
SMRiQ9LUM0. Positions 36-107, 392-665, 1494-1759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G14270.1.

PTM databases

iPTMnetiQ9LUM0.

Proteomic databases

PaxDbiQ9LUM0.
PRIDEiQ9LUM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14270.1; AT3G14270.1; AT3G14270.
GeneIDi820647.
GrameneiAT3G14270.1; AT3G14270.1; AT3G14270.
KEGGiath:AT3G14270.

Organism-specific databases

TAIRiAT3G14270.

Phylogenomic databases

eggNOGiKOG0230. Eukaryota.
COG0459. LUCA.
COG5253. LUCA.
HOGENOMiHOG000241144.
InParanoidiQ9LUM0.
KOiK00921.
OMAiRDECEAS.
PhylomeDBiQ9LUM0.

Enzyme and pathway databases

BioCyciARA:AT3G14270-MONOMER.
ReactomeiR-ATH-1660514. Synthesis of PIPs at the Golgi membrane.
R-ATH-1660516. Synthesis of PIPs at the early endosome membrane.
R-ATH-1660517. Synthesis of PIPs at the late endosome membrane.

Miscellaneous databases

PROiQ9LUM0.

Gene expression databases

GenevisibleiQ9LUM0. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
3.30.800.10. 1 hit.
3.30.810.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027483. PInositol-4-P-5-kinase_C.
IPR002498. PInositol-4-P-5-kinase_core.
IPR027484. PInositol-4-P-5-kinase_N.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
PF01363. FYVE. 1 hit.
PF01504. PIP5K. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00330. PIPKc. 1 hit.
[Graphical view]
SUPFAMiSSF52029. SSF52029. 1 hit.
SSF57903. SSF57903. 1 hit.
PROSITEiPS51455. PIPK. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-845.
    Strain: cv. Columbia.
  4. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
    Mueller-Roeber B., Pical C.
    Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, REVIEW.
  5. "Arabidopsis FAB1/PIKfyve proteins are essential for development of viable pollen."
    Whitley P., Hinz S., Doughty J.
    Plant Physiol. 151:1812-1822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  6. "Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis."
    Hirano T., Matsuzawa T., Takegawa K., Sato M.H.
    Plant Physiol. 155:797-807(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
  7. "Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters."
    Hirano T., Sato M.H.
    Plant Signal. Behav. 6:583-585(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiFAB1B_ARATH
AccessioniPrimary (citable) accession number: Q9LUM0
Secondary accession number(s): Q0WPY8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.