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Protein

1-phosphatidylinositol-3-phosphate 5-kinase FAB1B

Gene

FAB1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate (By similarity). Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.By similarity3 Publications

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate.

Cofactori

Mg2+By similarity, Mn2+By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri39 – 105FYVE-typePROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

  • 1-phosphatidylinositol-3-phosphate 5-kinase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • endomembrane system organization Source: TAIR
  • pollen development Source: TAIR
  • retrograde transport, endosome to Golgi Source: GO_Central
  • stomatal closure Source: TAIR
  • vacuole organization Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G14270-MONOMER
ReactomeiR-ATH-1660514 Synthesis of PIPs at the Golgi membrane
R-ATH-1660516 Synthesis of PIPs at the early endosome membrane
R-ATH-1660517 Synthesis of PIPs at the late endosome membrane

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol-3-phosphate 5-kinase FAB1B (EC:2.7.1.150)
Short name:
Phosphatidylinositol 3-phosphate 5-kinase
Alternative name(s):
FYVE finger-containing phosphoinositide kinase
PIKfyve
Phosphatidylinositol 3-phosphate 5-kinase type III
Short name:
PIPkin-III
Short name:
Type III PIP kinase
Protein FORMS APLOID AND BINUCLEATE CELLS 1B
Gene namesi
Name:FAB1B
Ordered Locus Names:At3g14270
ORF Names:MLN21.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G14270
TAIRilocus:2091050 AT3G14270

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Leaf-curling phenotype. Root growth inhibition, root gravitropic response, and hyposensitivity to exogenous auxin. Fab1a and fab1b double mutant displays an abnormal vacuolar phenotype late in pollen development leading to inviable pollen (PubMed:19846542).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004218711 – 17911-phosphatidylinositol-3-phosphate 5-kinase FAB1BAdd BLAST1791

Proteomic databases

PaxDbiQ9LUM0
PRIDEiQ9LUM0

PTM databases

iPTMnetiQ9LUM0

Expressioni

Tissue specificityi

Ubiquitous with highest expression levels in the root hair zone, pollen, and stamens.1 Publication

Gene expression databases

ExpressionAtlasiQ9LUM0 baseline and differential
GenevisibleiQ9LUM0 AT

Interactioni

Subunit structurei

Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G14270.1

Structurei

3D structure databases

ProteinModelPortaliQ9LUM0
SMRiQ9LUM0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1433 – 1758PIPKPROSITE-ProRule annotationAdd BLAST326

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1077 – 1111Sequence analysisAdd BLAST35

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri39 – 105FYVE-typePROSITE-ProRule annotationAdd BLAST67

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0230 Eukaryota
COG0459 LUCA
COG5253 LUCA
HOGENOMiHOG000241144
InParanoidiQ9LUM0
KOiK00921
OMAiWKKICHH
OrthoDBiEOG0936003B
PhylomeDBiQ9LUM0

Family and domain databases

Gene3Di3.30.40.10, 1 hit
3.30.800.10, 1 hit
3.50.7.10, 1 hit
InterProiView protein in InterPro
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR002498 PInositol-4-P-5-kinase_core
IPR027484 PInositol-4-P-5-kinase_N
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR11353 PTHR11353, 1 hit
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PF01363 FYVE, 1 hit
PF01504 PIP5K, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00330 PIPKc, 1 hit
SUPFAMiSSF52029 SSF52029, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51455 PIPK, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LUM0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTRDSNNRT FSEIVGLIKS WLPWRSEPAT VSRDFWMPDQ SCRVCYECDC
60 70 80 90 100
QFTLINRRHH CRHCGRVFCG KCTANSIPFA PSDLRTPRED WERIRVCNYC
110 120 130 140 150
FRQWEQGDGG PHVSNITELS TSPSETSLLS SKTSTTANSS SFALGSMPGL
160 170 180 190 200
IGLNQRVHHG SDVSLHGVSS METSVTKQGK ETSRRSSFIA TDVEDPSRFA
210 220 230 240 250
LNSIRSDDEY DEYGAYQTDI ETSHSPRANE YYGPMEYNGM GIDDVPCKHL
260 270 280 290 300
GGETADQKSL SGSPLIHQCL ESLIREGSEQ FQKKSEHDGR DECEASSPAD
310 320 330 340 350
ISDDQVVEPV DFENNGLLWV PPEPENEEDE RESALFDEED NEGDASGEWG
360 370 380 390 400
YLRPSTSFGS GEYRGEDRTT EEHKKAMKNV VDGHFRALLA QLLQVENISV
410 420 430 440 450
SDEEGKESWL EIITSLSWEA ANLLKPDMSK SGGMDPGGYV KVKCLASGFR
460 470 480 490 500
HDSMVVKGVV CKKNVVNRRM STKIEKARLL ILGGGLEYQR VSNQLSSFDT
510 520 530 540 550
LLQQEKDHLK MAVAKIHAER PNILLVEKSV SRFAQEYLLA KDISLVLNIK
560 570 580 590 600
RPLLDRIARC TGAQIIPSVD HLSSQKLGYC ENFRVDRYPE EHGSTGQVGK
610 620 630 640 650
KVVKTLMYFE HCPKPLGFTI LLRGANEDEL KKVKHVVQYG VFAAYHLALE
660 670 680 690 700
TSFLADEGAS PELPLNSPIT VALPDKSTSI ERSISTVPGF TVSTYEKSPT
710 720 730 740 750
MLSCAEPQRA NSVPVSELLS TTTNLSIQKD IPPIPYGSGW QAREINPSFV
760 770 780 790 800
FSRHNISLNL PDRVIESRNS DLSGRSVPVD TPADKSNPIV VADETTNNSL
810 820 830 840 850
HLSGQGFVRK SSQIGTSIMV ENQDNGSELT IAQQQNNEKP KETQSQKEEF
860 870 880 890 900
PPSPSDHQSI LVSLSSRSVW KGTVCERSHL FRIKYYGSFD KPLGRFLRDH
910 920 930 940 950
LFDQSYRCRS CEMPSEAHVH CYTHRQGSLT ISVKKLQDYL LPGEKEGKIW
960 970 980 990 1000
MWHRCLRCPR LNGFPPATLR VVMSDAAWGL SFGKFLELSF SNHAAASRVA
1010 1020 1030 1040 1050
CCGHSLHRDC LRFYGFGNMV ACFRYATIDV HSVYLPPSIL SFNYENQDWI
1060 1070 1080 1090 1100
QRETDEVIER AELLFSEVLN AISQIAEKGF RRRIGELEEV LQKEKAEFEE
1110 1120 1130 1140 1150
NMQKILHREV NEGQPLVDIL ELYRIHRQLL FQSYMWDHRL INASTLHKLE
1160 1170 1180 1190 1200
NSDDTKREEN EKPPLAKSQT LPEMNAGTNS LLTGSEVNLN PDGDSTGDTG
1210 1220 1230 1240 1250
SLNNVQKEAD TNSDLYQEKD DGGEVSPSKT LPDTSYPLEN KVDVRRTQSD
1260 1270 1280 1290 1300
GQIVMKNLSA TLDAAWIGER QTSVEIPTNN KVSLPPSTMS NSSTFPPISE
1310 1320 1330 1340 1350
GLMPIDLPEQ QNEFKVAYPV SPALPSKNYE NSEDSVSWLS VPFLNFYRSI
1360 1370 1380 1390 1400
NKNFLLSSQK LDTFGEHSPI YISSFREAEL QGGPRLLLPV GLNDIVVPVY
1410 1420 1430 1440 1450
DDEPTSMIAY ALMSPEYQRQ TSAEGESLVS YPSELNIPRP VDDTIFDPSR
1460 1470 1480 1490 1500
SNGSVDESIL SISSSRSTSL LDPLSYTKAL HARVSYGEDG TLGKVKYTVT
1510 1520 1530 1540 1550
CYYAKRFEAL RGICLPSELE YIRSLSRCKK WGAQGGKSNV FFAKTLDDRF
1560 1570 1580 1590 1600
IIKQVTKTEL ESFIKFAPAY FKYLSESIST KSPTCLAKIL GIYQVATKQL
1610 1620 1630 1640 1650
KSGKETKMDV LIMENLLFGR TVKRLYDLKG SSRARYNPDS SGSNKVLLDQ
1660 1670 1680 1690 1700
NLIEAMPTSP IFVGNKAKRL LERAVWNDTA FLALGDVMDY SLLVGVDEEK
1710 1720 1730 1740 1750
NELVLGIIDF LRQYTWDKHL ESWVKFTGIL GGPKNEAPTV ISPKQYKRRF
1760 1770 1780 1790
RKAMTTYFLM VPDQWSPPNV VANNSKSDQP EETSQAGTQA E
Length:1,791
Mass (Da):200,831
Last modified:October 1, 2000 - v1
Checksum:i859835D68E56039F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022220 Genomic DNA Translation: BAB01033.1
CP002686 Genomic DNA Translation: AEE75496.1
CP002686 Genomic DNA Translation: ANM65157.1
AK228922 mRNA Translation: BAF00811.1
RefSeqiNP_001319548.1, NM_001338116.1
NP_188044.1, NM_112285.4
UniGeneiAt.23226
At.71114

Genome annotation databases

EnsemblPlantsiAT3G14270.1; AT3G14270.1; AT3G14270
AT3G14270.2; AT3G14270.2; AT3G14270
GeneIDi820647
GrameneiAT3G14270.1; AT3G14270.1; AT3G14270
AT3G14270.2; AT3G14270.2; AT3G14270
KEGGiath:AT3G14270

Similar proteinsi

Entry informationi

Entry nameiFAB1B_ARATH
AccessioniPrimary (citable) accession number: Q9LUM0
Secondary accession number(s): Q0WPY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health