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Protein

Putative pectinesterase/pectinesterase inhibitor 26

Gene

PME26

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (At3g10720), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase 4 (PME4), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (At3g14310), Pectinesterase/pectinesterase inhibitor 18 (PME18), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Pectinesterase 2 (PME2), Probable pectinesterase 29 (PME29), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase 8 (PME8), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Putative pectinesterase 11 (PME11), Pectinesterase PPME1 (PPME1), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase, Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Probable pectinesterase 68 (PME68), Pectinesterase, Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Probable pectinesterase/pectinesterase inhibitor 6 (PME6)
  2. Pectate lyase (T26I12.20), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 6 (At2g02720), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase (At3g07010), Pectate lyase, Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (At5g04310), Pectate lyase (At4g13710), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Pectate lyase (At3g01270), Putative pectate lyase 11 (At3g27400), Pectate lyase (At5g04310), Pectate lyase (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei735 – 7351Substrate; for pectinesterase activityBy similarity
Binding sitei765 – 7651Substrate; for pectinesterase activityBy similarity
Sitei787 – 7871Transition state stabilizerBy similarity
Active sitei788 – 7881Proton donor; for pectinesterase activityPROSITE-ProRule annotation
Active sitei809 – 8091Nucleophile; for pectinesterase activityPROSITE-ProRule annotation
Binding sitei872 – 8721Substrate; for pectinesterase activityBy similarity
Binding sitei874 – 8741Substrate; for pectinesterase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT3G14300-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pectinesterase/pectinesterase inhibitor 26
Alternative name(s):
AtPMEpcrC
Including the following 2 domains:
Pectinesterase inhibitor 26
Alternative name(s):
Pectin methylesterase inhibitor 26
Pectinesterase 26 (EC:3.1.1.11)
Short name:
PE 26
Alternative name(s):
Pectin methylesterase 26
Short name:
AtPME26
Gene namesi
Name:PME26
Synonyms:ARATH26
Ordered Locus Names:At3g14300
ORF Names:MLN21.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G14300.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei33 – 5321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell periphery Source: GO_Central
  • cell wall Source: InterPro
  • integral component of membrane Source: UniProtKB-KW
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 968968Putative pectinesterase/pectinesterase inhibitor 26PRO_0000370189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence analysis
Glycosylationi158 – 1581N-linked (GlcNAc...)Sequence analysis
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence analysis
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence analysis
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence analysis
Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence analysis
Glycosylationi541 – 5411N-linked (GlcNAc...)Sequence analysis
Glycosylationi559 – 5591N-linked (GlcNAc...)Sequence analysis
Glycosylationi603 – 6031N-linked (GlcNAc...)Sequence analysis
Glycosylationi737 – 7371N-linked (GlcNAc...)Sequence analysis
Disulfide bondi802 ↔ 822By similarity
Glycosylationi863 – 8631N-linked (GlcNAc...)Sequence analysis
Glycosylationi900 – 9001N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9LUL8.
PRIDEiQ9LUL8.

Expressioni

Tissue specificityi

Expressed in flowers.1 Publication

Gene expression databases

GenevisibleiQ9LUL8. AT.

Structurei

3D structure databases

ProteinModelPortaliQ9LUL8.
SMRiQ9LUL8. Positions 271-395, 660-958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 230160Pectinesterase inhibitor 26 AAdd
BLAST
Regioni265 – 430166Pectinesterase inhibitor 26 BAdd
BLAST
Regioni453 – 614162Pectinesterase inhibitor 26 CAdd
BLAST
Regioni660 – 954295Pectinesterase 26Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4677. LUCA.
InParanoidiQ9LUL8.
PhylomeDBiQ9LUL8.

Family and domain databases

Gene3Di1.20.140.40. 3 hits.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 3 hits.
[Graphical view]
SMARTiSM00856. PMEI. 3 hits.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 3 hits.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 3 hits.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9LUL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTVKSINKG YGKVDETQDL ALKRKTRKRL YQIGISVAVL VAIIISSTVT
60 70 80 90 100
IAIHSRKGNS PHPTPSSVPE LTPAASLKTV CSVTNYPVSC FSSISKLPLS
110 120 130 140 150
NTTDPEVIFR LSLQVVIDEL NSIVELPKKL AEETDDEGLK SALSVCEHLL
160 170 180 190 200
DLAIDRVNET VSAMEVVDGK KILNAATIDD LLTWLSAAVT YHGTCLDALD
210 220 230 240 250
EISHTNSAIP LKLKSGMVNS TEFTSNSLAI VAKILSTISD FGIPIHGRRL
260 270 280 290 300
LNSSPHATPI SVPKLTPAAS LRNVCSVTRY PASCVSSISK LPSSNTTDPE
310 320 330 340 350
ALFRLSLQVV INELNSIAGL PKKLAEETDD ERLKSSLSVC GDVFNDAIDI
360 370 380 390 400
VNDTISTMEE VGDGKKILKS STIDEIQTWL SAAVTDHDTC LDALDELSQN
410 420 430 440 450
KTEYANSPIS LKLKSAMVNS RKFTSNSLAI IAKFPIHERH GVQSPRLRKS
460 470 480 490 500
PHPTPSSVLR TVCNVTNYPA SCISSISKLP LSKTTTDPKV LFRLSLQVTF
510 520 530 540 550
DELNSIVGLP KKLAEETNDE GLKSALSVCA DVFDLAVDSV NDTISSLDEV
560 570 580 590 600
ISGGKKNLNS STIGDLITWL SSAVTDIGTC GDTLDEDNYN SPIPQKLKSA
610 620 630 640 650
MVNSTEFTSN SLAIVAQVLK KPSKSRIPVQ GRRLLNSNSF PNWVRPGVRR
660 670 680 690 700
LLQAKNLTPH VTVAADGSGD VRTVNEAVWR VPKKGKTMFV IYVKAGTYVE
710 720 730 740 750
NVLMKKDKWN VFIYGDGRDK TIISGSTNMV DGVRTFNTST FATEGKGFMM
760 770 780 790 800
KDMGIINTAG PEKHQAVAFR SDSDRSVYYR CSFDGYQDTL YTHSNRQYYR
810 820 830 840 850
NCDVTGTVDF IFGAGTVVFQ GCSIRPRQPL PNQFNTITAE GTQEANQNTG
860 870 880 890 900
ISIHQCTISP NGNVTATTYL GRPWKLFSKT VIMQSVIGSF VNPAGWIAWN
910 920 930 940 950
STYDPPPRTI FYREYKNSGP GSDLSKRVKW AGYKPISSDD EAARFTVKYF
960
LRGDDNWIPK AVMGMPPL
Length:968
Mass (Da):105,628
Last modified:October 1, 2000 - v1
Checksum:i96DB0EAFAFA43898
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022220 Genomic DNA. Translation: BAB01036.1.
CP002686 Genomic DNA. Translation: AEE75499.1.
RefSeqiNP_188047.1. NM_112288.1.
UniGeneiAt.53309.

Genome annotation databases

EnsemblPlantsiAT3G14300.1; AT3G14300.1; AT3G14300.
GeneIDi820650.
GrameneiAT3G14300.1; AT3G14300.1; AT3G14300.
KEGGiath:AT3G14300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022220 Genomic DNA. Translation: BAB01036.1.
CP002686 Genomic DNA. Translation: AEE75499.1.
RefSeqiNP_188047.1. NM_112288.1.
UniGeneiAt.53309.

3D structure databases

ProteinModelPortaliQ9LUL8.
SMRiQ9LUL8. Positions 271-395, 660-958.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiQ9LUL8.
PRIDEiQ9LUL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14300.1; AT3G14300.1; AT3G14300.
GeneIDi820650.
GrameneiAT3G14300.1; AT3G14300.1; AT3G14300.
KEGGiath:AT3G14300.

Organism-specific databases

TAIRiAT3G14300.

Phylogenomic databases

eggNOGiCOG4677. LUCA.
InParanoidiQ9LUL8.
PhylomeDBiQ9LUL8.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT3G14300-MONOMER.

Miscellaneous databases

PROiQ9LUL8.

Gene expression databases

GenevisibleiQ9LUL8. AT.

Family and domain databases

Gene3Di1.20.140.40. 3 hits.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 3 hits.
[Graphical view]
SMARTiSM00856. PMEI. 3 hits.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 3 hits.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 3 hits.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
    Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
    DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Characterization of the pectin methylesterase-like gene AtPME3: a new member of a gene family comprising at least 12 genes in Arabidopsis thaliana."
    Micheli F., Holliger C., Goldberg R., Richard L.
    Gene 220:13-20(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Pectin methylesterases: sequence-structural features and phylogenetic relationships."
    Markovic O., Janecek S.
    Carbohydr. Res. 339:2281-2295(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPME26_ARATH
AccessioniPrimary (citable) accession number: Q9LUL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI regions may act as autoinhibitory domains and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.