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Q9LUI3 (TSO1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CRC domain-containing protein TSO1
Gene names
Name:TSO1
Ordered Locus Names:At3g22780
ORF Names:MWI23.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length695 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable floral-specific cell division component, required for proper organ formation in flowers. Regulates the floral meristem cell division and the inflorescence meristem organization. Plays a role in development of both male and female reproductive tissues. Ref.1 Ref.2 Ref.6 Ref.7

Subcellular location

Nucleus Ref.1 Ref.2.

Tissue specificity

Ubiquitous but expressed mostly in flowers with highest levels in developing ovules and microspores. Ref.1 Ref.2 Ref.8

Developmental stage

Expressed at the first stage of pollen development in uninucleate microspores and bicellular pollen but not in tricellular and mature pollen. Ref.8

Domain

The cysteine-rich domain CRC binds zinc in vitro.

Disruption phenotype

Despite a wild-type flower phenotype, the null mutant presents defects in pollen, carpel and ovule development, including a failure in megasporogenesis. Small siliques. Ref.7 Ref.8 Ref.9

Sequence similarities

Belongs to the lin-54 family.

Contains 1 CRC domain.

Sequence caution

The sequence AAK76693.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 695695CRC domain-containing protein TSO1
PRO_0000418166

Regions

Domain399 – 524126CRC
Motif269 – 28315Nuclear localization signal Potential

Experimental info

Mutagenesis4031C → Y: Abnormalities in floral meristems and sepals including increased cell size, decreased cell number, disorganized layer structure, increased DNA content per cell, and partially formed cell walls. Resulting phenotype is callus-like tissues in place of floral organs. Ref.1
Mutagenesis5011C → Y: Abnormalities in floral meristems and sepals including increased cell size, decreased cell number, disorganized layer structure, increased DNA content per cell, and partially formed cell walls. Resulting phenotype is callus-like tissues in place of floral organs. Ref.2
Sequence conflict1301M → L in AAF27433. Ref.1
Sequence conflict1301M → L in AAF69124. Ref.2
Sequence conflict3751V → M in AAK76693. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9LUI3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 34BBA0E450F6BCE1

FASTA69576,277
        10         20         30         40         50         60 
MDKSQKNPTS QIGTSTPKSK FEDSPVFNYI SNLSPIESVK SISTAQTFSS LSFTSPPPVF 

        70         80         90        100        110        120 
TSPHVISHRE SRFFRCHNSV DRSKHLESLD GSAVKGEVVV PLVEDLNKEA SLEDEEETSV 

       130        140        150        160        170        180 
ETSSELPQIM KFDSQTSEHS DSPCTEDVVI EASSDPPRGD NGSSSEDVTM GLQNMLVVRE 

       190        200        210        220        230        240 
GNDTPGCGRL ISDATELLVF RSPNDSEAFR CLVDKISSSE RRFCAGVKST KRPDINKDIP 

       250        260        270        280        290        300 
ANGSSNENQP LAVLPTNESV FNLHRGGMRR RCLDFEMPGK RKKDIVDDQQ SVCDNNVAGE 

       310        320        330        340        350        360 
SSSSCVVPGI GLHLNAVAMS AKDSNISVIH GYSISGEIQK SFSGSTTPIQ SQDTVQETSD 

       370        380        390        400        410        420 
QAENEPVEEV PKALVFPELN LGSLKKKMRK SEQAGEGESC KRCNCKKSKC LKLYCECFAA 

       430        440        450        460        470        480 
GVYCIEPCSC IDCFNKPIHE ETVLATRKQI ESRNPLAFAP KVIRNADSIM EASDDASKTP 

       490        500        510        520        530        540 
ASARHKRGCN CKKSNCMKKY CECYQGGVGC SMNCRCEGCT NVFGRKDGSL LVIMESKLEE 

       550        560        570        580        590        600 
NQETYEKRIA KIQHNVEVSK EVEQNPSSDQ PSTPLPPYRH LVVHQPFLSK NRLPPTQFFL 

       610        620        630        640        650        660 
GTGSSSFRKP NSDLAQSQNE KKPLETVTED KTEIMPEILL NSPIANIKAI SPNSKRVSPP 

       670        680        690 
QPGSSESGSI LRRRGNGRKL ILRSIPAFPS LNPNQ 

« Hide

References

« Hide 'large scale' references
[1]"Regulation of meristem organization and cell division by TSO1, an Arabidopsis gene with cysteine-rich repeats."
Song J.Y., Leung T., Ehler L.K., Wang C., Liu Z.
Development 127:2207-2217(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-403, SUBCELLULAR LOCATION.
Strain: cv. Landsberg erecta.
Tissue: Flower.
[2]"TSO1 is a novel protein that modulates cytokinesis and cell expansion in Arabidopsis."
Hauser B.A., He J.Q., Park S.O., Gasser C.S.
Development 127:2219-2226(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-501, SUBCELLULAR LOCATION.
Strain: cv. Landsberg erecta.
[3]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 160-695.
Strain: cv. Columbia.
[6]"TSO1 functions in cell division during Arabidopsis flower development."
Liu Z., Running M.P., Meyerowitz E.M.
Development 124:665-672(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Arabidopsis TSO1 regulates directional processes in cells during floral organogenesis."
Hauser B.A., Villanueva J.M., Gasser C.S.
Genetics 150:411-423(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]"The conserved cysteine-rich domain of a tesmin/TSO1-like protein binds zinc in vitro and TSO1 is required for both male and female fertility in Arabidopsis thaliana."
Andersen S.U., Algreen-Petersen R.G., Hoedl M., Jurkiewicz A., Cvitanich C., Braunschweig U., Schauser L., Oh S.A., Twell D., Jensen E.O.
J. Exp. Bot. 58:3657-3670(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, ZINC-BINDING, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[9]"Recessive antimorphic alleles overcome functionally redundant loci to reveal TSO1 function in Arabidopsis flowers and meristems."
Sijacic P., Wang W., Liu Z.
PLoS Genet. 7:E1002352-E1002352(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF206324 mRNA. Translation: AAF27433.1.
AF204059 mRNA. Translation: AAF69124.1.
AB022223 Genomic DNA. Translation: BAB01253.1.
CP002686 Genomic DNA. Translation: AEE76676.1.
AY046019 mRNA. Translation: AAK76693.1. Different initiation.
AY142642 mRNA. Translation: AAN13100.1.
RefSeqNP_566718.2. NM_113177.3.
UniGeneAt.24953.
At.68193.

3D structure databases

ProteinModelPortalQ9LUI3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G22780.1-P.

Proteomic databases

PRIDEQ9LUI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G22780.1; AT3G22780.1; AT3G22780.
GeneID821849.
KEGGath:AT3G22780.

Organism-specific databases

TAIRAT3G22780.

Phylogenomic databases

eggNOGNOG258818.
HOGENOMHOG000084455.
InParanoidQ9LUI3.
OMALVIMESK.
PhylomeDBQ9LUI3.
ProtClustDBCLSN2918188.

Gene expression databases

GenevestigatorQ9LUI3.

Family and domain databases

InterProIPR005172. CRC.
[Graphical view]
PfamPF03638. TCR. 2 hits.
[Graphical view]
PROSITEPS51634. CRC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTSO1_ARATH
AccessionPrimary (citable) accession number: Q9LUI3
Secondary accession number(s): Q8H1Q0, Q94AI2, Q9LE32
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names